• Title/Summary/Keyword: functional effect

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Antimicrobial Effect of Lactic Acid Bacteria Isolated from Kimchi and Tarak on Helicobacter pylori (김치와 타락에서 분리한 젖산균의 Helicobacterpylori에 대한 항균 효과)

  • Lee, Young-Duck;Yoo, Hye-Lim;Hwang, Ji-Yeon;Han, Bok-Kyung;Choi, Hyuk-Joon;Park, Jong-Hyun
    • The Korean Journal of Food And Nutrition
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    • v.23 no.4
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    • pp.664-669
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    • 2010
  • Lactic acid bacteria from traditional Korean foods of Tarak and Kimchi was isolated and characterized against carcinogenic Helicobacter pylori. Five Tarak and 30 Kimchi, traditional lactic acid-fermented foods, were collected from Andong area and the markets in Seoul, respectively and 15 lactic acid bacteria were isolated. Among them, two isolates were selected from high growth-inhibitory activities on H. pylori. The isolates were identified as Streptococcus thermophilus LAB kw15 from Tarak and Leuconostoc mesenteroides LAB kw5 from Kimchi by the biochemical characteristics and 16S DNA sequencing. The culture solutions of the isolates adjusted to pH 7.0 showed H. pylori inhibition. The isolates grew well and H. pylori did not grow during the co-culture with those strains. Therefore, L. mesenteroides LAB kw5 and S. thermophilus LAB kw15 might be the candidates as the functional lactic acid bacteria for improving stomach health.

The Effect of Total Patellectomy in the Prosthetic Replacement of Proximal Tibia (경골 근위부 종양에서 인공 삽입물 사용시 슬개골 전적출술이 관절기능 회복에 미치는 영향)

  • Park, Il-Hyung;Kim, Jae-Do;Ihn, Joo-Chul;Chun, In-Ho
    • The Journal of the Korean bone and joint tumor society
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    • v.2 no.1
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    • pp.8-17
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    • 1996
  • The purpose of this study is a comparative evaluation of range motion, especially extension deficit between the group of total patellectomy and that of intact patella, after reconstruction of the patellar tendon in the prosthetic replacement of a proximal tibia. Between 1990 and 1994, 15 patients who had a primary malignancy on proximal tibia were operated on. All patients were evaluated clinically and radiographically. Two patients were excluded because one had a deep infection treated with arthrodesis of the knee and the other was a composite allograft. The mean follow-up of the 13 patients was 27 months(15-47), including 10 osteosarcomas, 1 chondrosarcoma, 1 malignant fibrous histiocytoma and 1 malignant giant cell tumor. Eleven patients had a resection of the proximal tibia and 2 had an extracapsular total knee resection with distal femur. Reconstruction of the defect was done in 8 cases with a custom-made Link Endo-Model Total Rotation Knee Joint Prosthesis, and in 5 with How Medica Modular Resection System (HMRS). We used two methods to reconstruct the ligamentum patellae. Fixation of the patellar tendon to the prosthesis only with suturing and/or stapling(group SS) was done in 7. Transposition of gastrocnemius muscle to enhance fixation and to cover the prosthesis(group TG) was done in 6. Regardless of fixation methods, total patellectomy was done in 5 either to lengthen the patellar tendon or to make primary skin closure easier or for both. In 8 cases, patella was left intact or resurfaced with polyethylene prosthesis. Active extension was measured while the patient was in a sitting position. There is no statistically meaningful difference in terms of extension deficit (Wilcoxon rank test, p=0.8800) between patellectomy group and intact patella group, and between group of fixation only with suturing and that of gastrocnemius transposition. Two cases of extension deficit over 30 degree were seen in group SS and in the group of intact patella. Conclusively, total patellectomy could be an option without increasing the risk of extension deficit when primary skin closure is difficult or patellar tendon is a little bit short to be fixed. There is no rating in the Enneking system of functional evaluation that this finding into consideration.

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Effects of Artemisia capillaris Extracts on Intestinal Microflora In vitro and In vivo (In vitro 및 In vivo에서 인진쑥 추출물이 장내미생물에 미치는 영향)

  • Oh, Mi-Hyun;Kim, Kwang-Yup
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.39 no.11
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    • pp.1587-1594
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    • 2010
  • This study was designed to investigate the effect of Artemisia capillaris extracts on the intestinal microflora. In agar diffusion method, the solvent fractions of Artemisia capillaris showed growth inhibition against the intestinal microflora. In particular, the chloroform fraction of Artemisia capillaris had strong antibacterial activity against Clostridium perfringens, Clostridium difficile, Eubacterium limosum, and Bacteroides fragilis, but did not show antibacterial activity against Bifidobacterium bifidum and Lactobacillus acidophilus. Most chloroform fraction of Artemisia capillaris inhibitory activities were not reduced by heat treatment or pH variation against C. perfringens, C. difficile, E. limosum, and B. fragilis. MICs of the chloroform fraction were 1.25 mg/mL against C. perfringens, E. limosum and B. fragilis and 2.5 mg/mL against C. difficile. MBCs of chloroform fraction were 5 mg/mL against C. perfringens, E. limosum and 2.5 mg/mL against C. difficile, B. fragilis. The ethyl acetate fraction of Artemisia capillaris showed $3.08{\pm}0.03$ mg/10 mg total polyphenol and $1.91{\pm}0.03$ mg/10 mg total flavonoid contents. In vivo tests were performed to investigate the influence of Artemisia capillaris extract on the intestinal microflora in rats. The results showed the possibilities of utilizing Artemisia capillaris extracts as a functional food component to control intestinal microflora.

A Study on the Effect of Neurofeedback Traing on the hearing impairments Student about Resistance Stress (청각장애 학생들의 일상적 스트레스 저항능력에 뉴로피드백 훈련이 미치는 영향 분석 연구)

  • Bak, Ki-Ja
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.9
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    • pp.3318-3324
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    • 2010
  • This study was to examine the effectiveness of neurofeedback training by observing the pre and post brainwave measurement results of about 16(Primary Student 6, middle school student10) student who have shown resistance stress and body stress, mental stress. The study was examined at S city M dong A hearing impairments student, from Mar. 2008 and to Feb. 2009. The methodology used in the study is the Coloring Analysis Program of the Brain Quotient Test. As the brain waves are adjusted by timeseries linear analysis, the brain function quotients can reflect the functional states of the brain. The training took place two times a week, for about 40 minutes per session. The result confirmed the differences of both resistance stress quotient and body stress, mental stress. The results of the analysis show that neurofeedback training plays positive role in changing the brain function. Therefore, the result of the study shows that there is possibility that Neuro Feedback technique might be positively affecting resistance stress of the hearing impairments student, relationship between neurofeedback training and stress among several categories.

Estimation of C Storage and Annual $CO_2$ Uptake by Street Trees in Gyeonggi-do (경기도 도시가로수의 탄소저장량과 연간 이산화탄소 흡수량 산정)

  • Park, Eun-Jin;Kang, Kyu-Yi
    • Korean Journal of Environment and Ecology
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    • v.24 no.5
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    • pp.591-600
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    • 2010
  • We estimated and compared C storages and annual $CO_2$ uptakes by 9 dominant tree species planted along the streets. DBH and age by tree species were measured in the sites selected considering the planting status and distributions of tree species, and biomass, C storage, growth rate, and annual $CO_2$ uptake were estimated for each species. As a result, L. tulipifera, M. glyptostroboides, P. occidentalis were classified into fast-growing group, P. serrulata, G. biloba, Z. serrata, S. japonica, A. palmatum showed intermediate growth rates, and P. densiflora was slow-growing. Average C storage per tree was 205kgC/tree and ranged from 518kgC/tree(L. tulipifera) to 41kgC/tree(P. densiflora). Average annual $CO_2$ uptake by urban street trees over their lifespan ranged from $7.6kgCO_2$/tree/y to $99.1kgCO_2$/tree/y and L. tulipifera was the greatest, followed by glyptostroboides and P. occidentalis, and P. densiflora was the lowest. Total annual $CO_2$ uptake by all street trees in Gyeonggi-do, estimated based on the annual $CO_2$ uptake by each species, was as small as approximately 0.67% of that by forest in Gyeonggi-do. However, urban trees are still important because forest area continues to decrease and urbanization occurs annually in Gyeonggi-do, and should be managed considering their multi-functional aspects, including mitigation of heat island effect and building energy saving(indirect $CO_2$ uptake).

Studies on Heat Sensitivity of Egg Albumen III. Effects of Egg Albumen Concentration and Addition of Sugars on Heat Sensitivity of Egg Albumen (난백의 열감수성에 관한 연구 III. 난백의 농도와 당류의 첨가가 난백의 열감수성에 미치는 영향)

  • 유익종;김기성;송계원
    • Korean Journal of Poultry Science
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    • v.16 no.1
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    • pp.23-28
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    • 1989
  • This study was undertaken to find out the effect of eeg albumen concentration and addition of sugars on the functional properties of egg albumen before and after heat treatment at $60^{\circ}C$ for 5 minutes. The turbidity was decreased until 8.3% protein concentration but increased as diluted and decreased again below 3.32% protein concentration before and after the heat treatment. The foaming power was peak at 8.3% protein concentration but decreased as diluted before and after the heat treatment. The foam stability was decreased as diluted before and after the heat treatment. The turbidity was not changed by addition of sucrose before the heat treatment and decreased after the heat treatment. The foaming power was decreased by addition of over 5% sucrose before the heat treatment and decreased by addition of over 2.5% sucrose after the heat treatment . The foam stability was increased by addition of over 5% sucrose before the heat treatment and increased by addition of sucrose after the heat treatment. The turbidity was increased by addition of glucose before the heat treatment and not changed after the heat treatment. The foaming power was decreased by the addition of glucose before the heat treatment and decreased by the addition of over 5% glucose after the heat treatment. The foam stability was decreased by the addition of glucose before and after the heat treatment.

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Studies on Heat Sensitivity of Egg Albumen II. Effects of pH and/or the Addition of Metal ions on Heat Sensitivity of Egg Albumen (난백의 숙감수성에 관한 연구 II. 금속염의 첨가와 pH가 난백의 열감수성에 미치는 영향)

  • 유익종;이성기;김영붕
    • Korean Journal of Poultry Science
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    • v.16 no.1
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    • pp.17-22
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    • 1989
  • In order to dull heat sensitivity of egg albumen, metal ions (aluminium, ferric, ferrous, copper) were added and functional properties or egg albumen were determined before and after heat treatment at $60^{\circ}C$ for 5 minutes. Effect of pH on heat sensitivity of aluminium salt added egg albumen was also determined. Addition of metal ions increased turbidity of egg albumen before and after the heat treatment. Changes of the turbidity were minimized by addition of aluminium salt. The foaming power was markedly increased by addition of ferric salt before the heat treatment and increased by addition of aluminium, ferric and copper salt after the heat treatment. Before the heat treatment the foam was stable by addition of ferric and ferrous salt but after the heat treatment it was stable by addition of aluminium and ferric salt. The turbidity and foaming property of the egg albumen with aluminium salt were not largely changed after the heat treatment at pH range 7 to 8.5. Over pH 9 the turbidity and foaming power were not decreased, but the foam stability was increased before and after the heat treatment. Salmonella typhimurium ATCC 14028 (10$^{6}$ cells/$m\ell$) inoculated in egg albumen at pH range 7 to 8.5 was destructed by the heat treatment.

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Regulation of SPIN90 by Cell Adhesion and ERK Activation

  • Kim Sung Hyun;Kim Dae Joong;Song Woo Keun
    • Proceedings of the Microbiological Society of Korea Conference
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    • 2004.05a
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    • pp.141-146
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    • 2004
  • SPIN90 was identified to farm molecular complex with $\betaPIX$, WASP and Nck. This complex shows that SPIN90 interacts with Nck in a manner dependent upon cell adhesion to extracellular matrix, but $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex was stable even in suspended cells. This suggests that SPIN90 serves as an adaptor molecule to recruit other proteins to Nck at focal adhesions. SPIN90 was phosphorylated by ERK1, which was, itself, activated by cell adhesion and platelet-derived growth factor. Such phosphorylation of SPIN90 likely promotes the interaction of the $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex and Nck. It thus appears that the interaction of the $SPIN90{\cdot}{\beta}PIX{\cdot}WASP$ complex with Nck is crucial for stable cell adhesion and can be dynamically modulated by SPIN90 phosphorylation that is dependent on cell adhesion and ERX activation. SPIN90 directly binds syndapin I, syndapin isoform II-1 and II-s via its PRD region in vitro, in vivo and also associates with endocytosis core components such as clathrin and dynamin. In neuron and fibroblast, SPIN90 colocalizes with syndapins as puntate form, consistent with a role for SPIN90 in clathrin-mediated endocytosis pathway. Overexpression of SPIN90 N-term inhibits receptor-mediated endocytosis. Interestingly, SPIN90 PRD, binding interface of syndapin, significantly blocks internalization of transferrin, demonstrating SPIN90 involvement in endocytosis in vivo by interacting syndapin. Depletion of endogenous SPIN90 by introducing $\alpha-SPIN90$ also blocks receptor-mediated endocytosis. Actin polymerization could generate farce facilitating the pinch-out event in endocytosis, detach newly formed endocytic vesicle from the plasma membrane or push out them via the cytosol on actin tails. Here we found that SPIN90 localizes to high actin turn over cortical area, actin-membrane interface and membrane ruffle in PDGF treated cells. Overexpression of SPIN90 has an effect on cortical actin rearrangement as filopodia induction and it is mediated by the Arp2/3 complex at cell periphery. Consistent with a role in actin organization, CFP-SPIN90 present in actin comet tail generated by PIP5 $kinase\gamma$ overexpression. Therefore this study suggests that SPIN90 is functional linker between endocytosis and actin cytoskeleton.

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Chemical Components and Physiological Activities of Neungee Mushroom (Sarcodon aspratus) (능이버섯(Sarcodon aspratus)의 화학성분과 생리활성)

  • Joo, Ok-Soo
    • Food Science and Preservation
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    • v.15 no.6
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    • pp.864-871
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    • 2008
  • The chemical components and physiological activities of Neungee mushroom (Sarcodon aspratus) were investigated to assess its nutritional and functional value. The moisture, total protein, crude fat and ash contents of Neungee mushroom were 85.73%, 1.78%, 1.87% and 1.27%, respectively. The alanine, linoleic acid, tartaric acid and glucose concentrations in Neungee mushrooms were 90.11, 39.09, 75.47 and 1,680 mg%, respectively. The radical and nitrate scavenging activities in Neungee mushroom extracts were 46.2% and 77.8% on $800{\mu}g/mL$ depending on the extract concentration. The lipid peroxidation inhibitory effect of Neungee mushroom extract ($1,500\;{\mu}g/mL$) was $2,347\;{\mu}mol$ MDA/g liver. We also observed that an extract concentration of $1,500\;{\mu}g/mL$ was more effective than the control at 7 d. The cytotoxicity of the Neungee mushroom extract ($100\;{\mu}g/mL$) for the A549 (lung carcinoma) cells was 96.0%.

A Case of Multiple Symmetric Lipomatosis (다발성대칭성지방종증의 1예)

  • Yoon, Su-Mi;Kim, Yeon-Soo;Kim, Jin-Ho;Lee, Dong-Suck;Choi, Jin-Won;Yang, Seok-Chul;Yoon, Ho-Joo;Shin, Dong-Ho;Kim, Tae-Wha;Choi, Yo-Won;Park, Sung-Soo
    • Tuberculosis and Respiratory Diseases
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    • v.47 no.1
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    • pp.97-102
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    • 1999
  • Multiple symmetric lipomatosis(Madelung's disease) seems to represent a relatively common disease in Europe but has not been described in the Korean literature. It is characterized by highly typical symmetric deposits of subcutaneous fat on the neck, arms, and upper part of the trunk. The localized increase of adipose tissue is hyperplastic in nature and probably results from a local defect in adrenergic stimulated lipolysis. Multiple symmetric lipomatosis is frequently, but inconstantly, associated with alcoholism, liver disease, glucose intolerance, and hyperuricemia, but their etiologic role remains to be established. Clinical importance is the apparent high incidence of malignant tumors of the upper airways requiring through ear, nose, and throat and internal examination of all patients. Another importance is possibility of airway obstruction due to mass effect. Dietary treatment and weight loss are of limited value. Surgical treatment gives only temporary relief and should be restricted to patients with functional impairment. We report a case of a 63-year-old man with multiple symetric lipomatosis and alcoholism.

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