Chemical Components and Physiological Activities of Neungee Mushroom (Sarcodon aspratus)

능이버섯(Sarcodon aspratus)의 화학성분과 생리활성

  • Joo, Ok-Soo (Department of Food Science, Jinju National University)
  • 주옥수 (진주산업대학교 식품과학과)
  • Published : 2008.12.31

Abstract

The chemical components and physiological activities of Neungee mushroom (Sarcodon aspratus) were investigated to assess its nutritional and functional value. The moisture, total protein, crude fat and ash contents of Neungee mushroom were 85.73%, 1.78%, 1.87% and 1.27%, respectively. The alanine, linoleic acid, tartaric acid and glucose concentrations in Neungee mushrooms were 90.11, 39.09, 75.47 and 1,680 mg%, respectively. The radical and nitrate scavenging activities in Neungee mushroom extracts were 46.2% and 77.8% on $800{\mu}g/mL$ depending on the extract concentration. The lipid peroxidation inhibitory effect of Neungee mushroom extract ($1,500\;{\mu}g/mL$) was $2,347\;{\mu}mol$ MDA/g liver. We also observed that an extract concentration of $1,500\;{\mu}g/mL$ was more effective than the control at 7 d. The cytotoxicity of the Neungee mushroom extract ($100\;{\mu}g/mL$) for the A549 (lung carcinoma) cells was 96.0%.

본 연구는 능이버섯의 식품영양학적 및 기능성을 평가하기 위하여 능이버섯의 화학성분과 추출물에 대한 생리활성을 확인하였다. 능이버섯은 수분 85.73%, 조단백질 1.78%, 조지질 1.87%, 회분 1.27%로 나타났으며, 아미노산 중 alanine(90.11 mg%), 지방산 중 linoleic acid(39.09 mg%), 유기산 중 tartaric acid(75.47 mg%) 및 유리당 중 glucose (1,680 mg%)의 함량들이 가장 높게 나타났다. 능이버섯 추출물에 대한 전자 소거능 및 아질산염 소거능은 농도가 증가함에 따라 활성이 증가하였고, $800\;{\mu}g/mL$ 처리 시 각각 46.2% 및 77.8%로 가장 높게 나타났다. 능이버섯 추출물은 역시 농도가 증가함에 따라 간 지질에 대한 과산화 억제 활성이 증가하였고, $1,500\;{\mu}g/mL$ 처리 시 $2,347\;{\mu}mol$ MDA/g liver로 가장 높게 나타났다. 능이버섯추출물을 첨가하여 linoleic acid에 대한 항산화 활성을 살펴본 결과, 저장 7일 후에 POV가 197.4 meq/kg이하로서 대조구에 비하여 약간의 항산화효과가 있었다. 암세포에 대한 능이버섯 메탄올 추출물의 세포독성에서, 폐암 세포주인 A549에 대한 증식 억제율은 능이버섯 메탄올 추출물 $100\;{\mu}g/mL$ 처리시 96%로 가장 높게 나타났다.

Keywords

References

  1. Yu, H.E., Cho, S.M., Seo, G.S., Lee, B.S., Lee, D.H. and Lee, J.S. (2006) Screening of bioactive compounds from mushroom Pholiota sp. Korean J. Mycol., 34, 15-21 https://doi.org/10.4489/KJM.2006.34.1.015
  2. Kim, M.C., Kim, J.S. and Heo, M.S. (2008) Antibacterial, antioxidant and autitumor activities of mushroom mycelium mixed culture extracts. Korean J. Biotechnol. Bioeng., 23, 158-163
  3. Lucas, E.H., Ringle, R.U., Clarke, D.A., Reilly, H.C., Stevens, J.A. and Stock, C.C. (1957) Tumor inhibitors in Boletus edulis and other Holobasidiomycetes. Antibiot. Chemotherapy, 7, 1-4
  4. Lee, J.W, Chung, C.H., Jeoung, H.J. and Lee, K.H. (1990) Anticomplementary and antitumor activities of the ikal. extract from the mycelia of Lentinus edodes IY-105. J. Appl. Microbiol. Biotechnol., 18, 571-577
  5. Lee, K.H., Kwon, H.J., Chun, S.S., Kim, J.H., Cho, Y.J. and Cha, W.S. (2006) Biological activities of extracts from Phelinus linteus. J. Korean Soc. Appl. Biol. Chem., 49, 298-303
  6. Ji, J.H., Kim, M.N., Choi, K.P., Chung, C.K. and Ham, S.S. (2000) Antimutagenic and cytotoxicity effects of Agaricus blazei Murill extracts. Korean J. Food Sci. Technol., 32, 1371-1378
  7. Sone, Y., Okuda, R., Wada, N., Kishia, E. and Misaki, A. (1985) Structures and antitumor activities of the polysaccharides isolated from fruiting body and the growing culture of mycelium of Ganoderma lucidium Agric. Biol. Chem., 49, 2641 https://doi.org/10.1271/bbb1961.49.2641
  8. Park, K.J., Oh, Y.J., Lee, S.Y., Kim, H.S. and Ha, H.C. (2007) Anti-diabetic effect of crude polysaccharides from Grifola frondosa in KK-A diabetic mouse and 3T3-L1 adipocyte. Korean J. Food Sci. Technol., 39, 330-335
  9. Chung, H.K. and Lee, J.W. (2001) Biological activities of substance extracted from the fruit body of Formitopsis rosea. Korean J. Food Sci. Technol., 33, 122-127
  10. Zhang, J., Wang, G., Li, H., Zhuang, C., Mizuno, T., Ito, H., Suzuki, C., Okamoto, H. and Li, J. (1994) Antitumor polysaccharides from a chinease mushroom "Yunhangmo", the fruiting body of Pleurotus citrinopileatus. Biosci. Biotechnol. Biochem., 58, 1195-1201 https://doi.org/10.1271/bbb.58.1195
  11. Hong, K.H., Kim, B.Y. and Kim, H.K. (2004) Studies on the biological activity of Pleurotus ferulea. J. Korean Soc. Food Sci. Nutr., 33, 791-796 https://doi.org/10.3746/jkfn.2004.33.5.791
  12. Park, M.H., Oh, K.Y. and Lee, B.W. (1998) Anticancer activity of Lentinus edodes and Pleurotus astreatus. Korean J. Food Sci. Technol., 30, 702-708
  13. Kim, H.K., Han, H.S., Lee, G.D. and Kim, K.H. (2005) Physiological activities of fresh Pleurotus eryngii extracts. J. Korean Soc. Food Sci. Nutr., 34, 439-445 https://doi.org/10.3746/jkfn.2005.34.4.439
  14. Fjui, T., Maeda, H., Suzuki, F. and Ishida, N. (1978) Isolation and characterization of a new antitumor polysaccharide, KS-2, extracted from culture mycelia of Lentinus edodes L. J. Antibiot, 31, 1079-1085 https://doi.org/10.7164/antibiotics.31.1079
  15. Lee, S.H., Kim, N.W. and Shin, S.R. (2003) Studies on the nutritional components of mushroom (Sarcodon aspratus). Korean J. Food Preserv., 10, 65-69
  16. Song, J.H., Lee, H.S., Hwang, J.K., Han, J.W., Ro, J.G., Keum, D.H. and Park, K.M. (2003) Physiological activity of Sarcodon aspratus extracts. Korean J. Food Sci. Ani. Resour., 23, 172-179
  17. Eun, J.S., Yang, J.H., Cho, D.Y. and Lee, T.K. (1988) Studies on higher fungi in Korea (I) : Activity of proteolytic enzyme from Sarcodon aspratus. J. Korean Pharm. Sci., 18, 125-131
  18. Jeong, O.J., Yoon, H.S. and Min, Y.K. (2001) Aroma characteristics of Neungee (Sarcodon aspratus). Korean J. Food Sci. Technol., 33, 307-312
  19. Kang, H.C., Yun, B.S., Yu, S.H. and Yoo, I.D. (2000) Chemical structures of the compounds isolated from the edible mushroom Sarcodon aspratus. J. Korean Soc. Agric. Chem. Biotechnol., 43, 298-302
  20. Kim, J.W., Moon, B.S., Park, Y.M., Yoo, N.H., Ryoo, I.J., Chinh, N.T., Yoo, I.D. and Kim, J.P. (2005) Structures and antioxidant activity of diketopiperazines isolated from the mushroom Sarcodon aspratus. J. Korean Soc. Agric. Biol. Chem., 48, 93-97
  21. Joo, H.K., Cho, H.K., Park, C.K., Cho, K.S., Choi, S.K. and Ma, S.J. (1989) Food Analysis. Yurim Munhwasa, Seoul, p.135-200
  22. Park, S.K., Seo, K.I., Choi, S.H., Moon, J.S. and Lee, Y.H. (2000) Quality assessment of commercial doenjang prepared by traditional method. J. Korean Soc. Food Sci. Nutr., 29, 211-217
  23. Cho, K.M., Lee, J.B., Kahng, G.G. and Seo, W.T. (2006) A study on the making of sweet persimmon (Diospyros kaki, T) wine. Korean J. Food Sci Technol., 38, 785-792
  24. Blois, M.S. (1958) Antioxidant determinations by the use of stable free radical. Nature, 181, 1199-1120 https://doi.org/10.1038/1811199a0
  25. Gray, J.I. and Dugan, L.R. (1975) Inhibition of N-nitrisiamine formation in model food systems. J. Food Sci., 40, 981-986 https://doi.org/10.1111/j.1365-2621.1975.tb02248.x
  26. Masugi, F. and Nakamura, T. (1997) Measurement of thiobarbituric acid value in liver homogenate solubilized with sodium dodecylsulphate andvariation of the values affected by vitamin E and drugs. Vitamin, 51, 21-29
  27. Kiharu, I., Miho, I. and Toshimi, H. (1990) Major antioxidative substances in leaves of astsumi-kabu. Agric. Biol. Chem., 54, 1053-1055 https://doi.org/10.1271/bbb1961.54.1053
  28. Skehan, P., Storeng, R., Monks, S.A., McMahan, J., Vistica, D., Warren, J.T., Bokesch, H., Kenny, S. and Boyd, M.R. (1990) New colorimetric cytoxicity assay for cancer-drug screening. J. Natl. Cancer Inst., 82, 1107-1112 https://doi.org/10.1093/jnci/82.13.1107
  29. Park, W.H. (1998) Studies on inorganic components of Korean mushrooms (1) - Inorganic components of Laccaria laccata, Laccaria amethystina, Laccria tortilis and Laccria vinaceoavellanea. Korean J. Mycol., 16, 242-246
  30. Seoh, J.H., Cho, S.Y. and Lee, S.W. (1974) Study on the tasty constituents and minerals in clavariaceae botrytis. J. Korean Soc. Food Sci. Nutr., 3, 17-21
  31. Hideo, K., Sugahara, T., Matsuzawa, M., Sumiyahsiki, K., Aoyagi, Y. and Hosogai, Y. (1986) Mineral contents in edible mushrooms. Nippon Shokuhin Kogyo Gakkaishi, 33, 250-255 https://doi.org/10.3136/nskkk1962.33.4_250
  32. Chung, D.S., Son, Y.K., Lee, Y.I., Yoon, I.W. and Kim, J.K. (1986) Studies on the chemical compoents, extracting and processing for an instant tea of Ganoderma lucidum. Agirc. Sci. Inst., 28, 140-145
  33. Lee, Y.S., Kim, J.B., Shin, S.R. and Kim, N.W. (2006) Analysis of nutritional components of Lepista nuda. Korean J. Food Preserv., 13, 375-381
  34. Lee, G.D., Chang, H.K. and Kim, H.K. (1997) Antioxidative and nitrite-scavenging activities of edible mushrooms. Korean J. Food Sci. Technol., 29, 432-436
  35. Park, S.S., Yu, K.H. and Min, T.J. (1998) Antioxidant activities of extracts from fruiting bodies of mushrooms. Korean J. Mycol., 26, 69-77
  36. Matsuzawa, T., Sano, M., Tomita, I., Saitoh, H. and Ikeda, T. (1997) Studies on antioxidants of Hypsizigus marmoreus. I. Effects of Hypsizigus marmoreus for antioxidants activities of mice plasma. Yakugaku Zasshi, 117, 623-628 https://doi.org/10.1248/yakushi1947.117.9_623
  37. Jung, I.C., Park, S., Park, K.S. and Ha, H.C. (1996) Antioxidative effect of fruit body and mycelial extracts of Pleurotus ostreatus. Korean J. Food Sci. Technol., 28, 464-469