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Identification and structural analysis of novel laccase genes in Flammulina elastica genome (Flammulina elastica의 유전체 정보기반 신규 laccase 유전자 동정 및 구조 분석)

  • Yu, Hye-Won;Park, Young-Jin
    • Journal of Mushroom
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    • v.19 no.1
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    • pp.33-40
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    • 2021
  • The genome sequence of various Flammulina species has recently been reported, thereby revealing a diverse genetic repertoire. In this study, we identified laccase genes and analyzed their structural characteristics in Flammulina elastica (F. elastica) genome. Through genome analysis and bioinformatics approaches, three laccase genes (Fe-lac1, -lac2, and -lac3) were identified, ranging from 1,548 to 1,602 bp in length. These genes contained a copper ion-binding region with ten histidine residues and one cysteine residue and a disulfide bond-forming region with four cysteine residues. Full-length cDNA sequencing analysis revealed that laccase genes contain 12 to 16 introns and signal peptides between 17 and 22 bp in the N-terminus. Structural characterization of the laccase genes identified in this study should help in better understanding the biomass decomposition of F. elastica.

Effect of Accelerated Weathering on the Leaching of Copper from Preservative Treated Wood (기상열화가 방부처리재의 구리성분 용탈에 미치는 영향)

  • Lee, Myung-Jae;Lee, Dong-Heub;Kim, Gyu-Hyeok
    • Journal of the Korean Wood Science and Technology
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    • v.31 no.4
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    • pp.38-43
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    • 2003
  • This is the second in a series of reports on the evaluation of weathering durability of waterborne preservative treated wood by accelerated weathering. The leaching of copper from ACQ-, CCA-, and CuAz-treated samples during weathering was increased by UV irradiation, when compared between full weathering and water-only weathering. The FTIR spectra of the weathered ACQ-, CCA, CuAz-treated samples at 1731 cm-1, 1625 cm-1, 1510 cm-1, which are related to the fixation of copper, were different from those of unweathered controls. This result means that UV irradiation can weaken the chemical bond between lignin and copper and/or extraction of lignin-copper complex can be occured when lignin erode away by weathering.

Seismic performance of CFS shear wall systems filled with polystyrene lightweight concrete: Experimental investigation and design methodology

  • Mohammad Rezaeian Pakizeh;Hossein Parastesh;Iman Hajirasouliha;Farhang Farahbod
    • Steel and Composite Structures
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    • v.46 no.4
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    • pp.497-512
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    • 2023
  • Using light weight concrete as infill material in conventional cold-formed steel (CFS) shear wall systems can considerably increase their load bearing capacity, ductility, integrity and fire resistance. The compressive strength of the filler concrete is a key factor affecting the structural behaviour of the composite wall systems, and therefore, achieving maximum compressive strength in lightweight concrete while maintaining its lightweight properties is of significant importance. In this study a new type of optimum polystyrene lightweight concrete (OPLC) with high compressive strength is developed for infill material in composite CFS shear wall systems. To study the seismic behaviour of the OPLC-filled CFS shear wall systems, two full scale wall specimens are tested under cyclic loading condition. The effects of OPLC on load-bearing capacity, failure mode, ductility, energy dissipation capacity, and stiffness degradation of the walls are investigated. It is shown that the use of OPLC as infill in CFS shear walls can considerably improve their seismic performance by: (i) preventing the premature buckling of the stud members, and (ii) changing the dominant failure mode from brittle to ductile thanks to the bond-slip behaviour between OPLC and CFS studs. It is also shown that the design equations proposed by EC8 and ACI 318-14 standards overestimate the shear force capacity of OPLC-filled CFS shear wall systems by up to 80%. This shows it is necessary to propose methods with higher efficiency to predict the capacity of these systems for practical applications.

Investigating the load-displacement restorative force model for steel slag self-stressing concrete-filled circular steel tubular columns

  • Feng Yu;Bo Xu;Chi Yao;Alei Dong;Yuan Fang
    • Steel and Composite Structures
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    • v.49 no.6
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    • pp.615-631
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    • 2023
  • To investigate the seismic behavior of steel slag self-stressing concrete-filled circular steel tubular (SSSCFCST) columns, 14 specimens were designed, namely, 10 SSSCFCST columns and four ordinary steel slag (SS) concrete (SSC)-filled circular steel tubular (SSCFCST) columns. Comparative tests were conducted under low reversed cyclic loading considering various parameters, such as the axial compression ratio, diameter-thickness ratio, shear-span ratio, and expansion ratio of SSC. The failure process of the specimens was observed, and hysteretic and skeleton curves were obtained. Next, the influence of these parameters on the hysteretic behavior of the SSSCFCST columns was analyzed. The self stress of SS considerably increased the bearing capacity and ductility of the specimens. Results indicated that specimens with a shear-span ratio of 1.83 exhibited compression bending failure, whereas those with shear-span ratios of 0.91 or 1.37 exhibited drum-shaped cracking failure. However, shear-bond failure occurred in the nonloading direction. The stiffness of the falling section of the specimens decreased with increasing shear-span ratio. The hysteretic curves exhibited a weak pinch phenomenon, and their shapes evolved from a full shuttle shape to a bow shape during loading. The skeleton curves of the specimens were nearly complete, progressing through elastic, elastoplastic, and plastic stages. Based on the experimental study and considering the effects of the SSC expansion rate, shear-span ratio, diameter-thickness ratio, and axial compression ratio on the seismic behavior, a peak displacement coefficient of 0.91 was introduced through regression analysis. A simplified method for calculating load-displacement skeleton curves was proposed and loading and unloading rules for SSSCFCST columns were provided. The load-displacement restorative force model of the specimens was established. These findings can serve as a guide for further research and practical application of SSSCFCST columns.

Type Variations of 'Stepmother' and 'Sister' in the Novels of Park Kyong-Ni and Their Meanings -Focused on Jaegwiyeol, Eunha, Kimyakgukeue Ddaldeul, Nabiwa Unggungkwi (박경리 장편소설의 '계모'·'자매' 유형 변화와 그 의미 -『재귀열』, 『은하』, 『김약국의 딸들』, 『나비와 엉겅퀴』를 중심으로)

  • Cho, Yun-A
    • Journal of Popular Narrative
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    • v.26 no.4
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    • pp.145-181
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    • 2020
  • This study analyzes type variations of the 'stepmother' and 'sister' in the full-length novels of Park Kyong-Ni and attempted to point out their meanings. The pattern of "negative stepmother" that appeared in classical and new novels also appeared repeatedly in Park Kyong-Ni's full-length novels and this was because a change took place in later full-length novels. Novels analyzed with focus were Jaegwiyeol(1959), Eunha(1960), Kimyakgukeue Ddaldeul(1962), and Nabiwa Unggungkwi(1969). The stepmother that appears in Eunha is a type that appears often in the classic and new novels of Korea. While the stepmother newly gained the role and status of 'mother', she forms a competitive relationship with the daughter of the former wife while still refusing to be a member of the family and she puts the former wife's daughter in critical situations by committing misdeeds. However, the young stepmother in Nabiwa Unggungkwi actually becomes a victim to the malicious and morbid harassment of the former wife's daughter. This stepmother is a good-natured figure who shows a sense of guilt for failing to fulfill her responsibilities of upbringing and education and she eventually dies as a victim to a bomb during the war, leaving her young biological daughter behind. On one hand, the sisters in Jaegwiyeol and Kimyakgukeue Ddaldeul are not strongly bonded but when one is caught in a crisis, the other one claims to be of help. Unlike this, the sisters in Nabiwa Unggungkwi have a bond that cannot be broken. They are half-sisters that bind each other so severely that they hinder each other's growth and they eventually end up disintegrating. Through such analyses, it is shown that issues of human nature are dealt with more acutely by breaking the 'young stepmother' away from convention by placing her in the position of the victim to amplify the conflicting relationship between sisters, unlike in previous pieces. This study was significant in that it looked into how previously repetitive character type changes appeared in full-length novels in conditions that clearly display the writer's determination to leave behind a masterpiece.

Quantum Chemical Calculations of the Effect of Si-O Bond Length on X-ray Raman Scattering Features for MgSiO3 Perovskite (양자화학계산을 이용한 Si-O 결합길이가 MgSiO3 페로브스카이트의 X-선 Raman 산란 스펙트럼에 미치는 영향에 대한 연구)

  • Yi, Yoo Soo;Lee, Sung Keun
    • Journal of the Mineralogical Society of Korea
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    • v.27 no.1
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    • pp.1-15
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    • 2014
  • Probing the electronic structures of crystalline Mg-silicates at high pressure is essential for understanding the various macroscopic properties of mantle materials in Earth's interior. Quantum chemical calculations based on the density functional theory are used to explore the atomic configuration and electronic structures of Earth materials at high pressure. Here, we calculate the partial density of states (PDOS) and O K-edge energy-loss near-edge structure (ELNES) spectra for $MgSiO_3$ perovskite at 25 GPa and 120 GPa using the WIEN2k program based on the full-potential linearized projected augmented wave (FP-LPAW) method. The calculated PDOS and O K-edge ELNES spectra for $MgSiO_3$ Pv show significant pressure-induced changes in their characteristic spectral features and relative peak intensity. These changes in spectral features of $MgSiO_3$ Pv indicate that the pressure-induced changes in local atomic configuration around O atoms such as Si-O, O-O, and Mg-O length can induce the significant changes on the local electronic structures around O atoms. The result also indicates that the significant changes in O K-edge features can results from the topological densification at constant Si coordination number. This study can provide a unique opportunity to understand the atomistic origins of pressure-induced changes in local electronic structures of crystalline and amorphous $MgSiO_3$ at high pressure more systematically.

The Effect of Anchorage with Shear Reinforcement in Flat Plate System (플랫 플레이트 구조에서 전단보강체의 정착성능에 따른 전단보강효과)

  • Choi, Chang-Sik;Bae, Baek-Il;Choi, Yun-Cheul;Choi, Hyun-Ki
    • Journal of the Korea Concrete Institute
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    • v.24 no.6
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    • pp.667-675
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    • 2012
  • Flat plate are being used more in buildings requiring a high level of technical installations or in buildings needing changeable room arrangements during their life time such as office buildings. The main problem in flat plate is its weak resistance against a punching failure at its slab-column connections. Therefore, in this research, an experimental study on full-scale interior slab-column connection was performed. Three types of shear reinforcements were tested to prevent brittle punching shear failure that could lead to collapse of the structure. A series of four flat plate specimens including a specimen without shear reinforcement and three specimens with shear reinforcements were tested. The slabs were tested up to failure using monotonic vertical shear loading. The presences of the shear reinforcements substantially increased punching shear capacity and ductility of the interior slabcolumn connections. The test results showed that a slab that did not have enough bond length failed before shear reinforcement yielded due to anchorage slip. Also, FEM analyses were performed to study an effect of slab thickness and concrete compressive strength on the flat plate slab. The analytical study results were used to propose a method to calculate performance capacity of shear reinforcement in slab-column connection.

Infulence of Spacer and Degree of Esterification on Thermotropic Liquid Crystalline Properties of Amyloses Bearing Cholesteryl Group (스페이서와 에스터화도가 콜레스테릴 그룹을 지닌 아밀로오스들의 열방성 액정 특성에 미치는 영향)

  • Jeong, Seung-Yong;Ma, Yung-Dae
    • Polymer(Korea)
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    • v.31 no.4
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    • pp.356-367
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    • 2007
  • Three kinds of amylose derivatives such as: cholesteryloxycarbonated amyloses(CAMs) with degree of esterification(DE) ranging from 1.8 to 3, (6-cholesteryloxycarbonyl)pentanoated amyloses(PAMs) with DE ranging from 0.3 to 3, and fully cholesteryloxycarbonated PAMs(CPAMs) were synthesized, and their thermotropic liquid crystalline properties were investigated. CAMs with $DE{\geq}2.6$, PAM with DE=1.6 and all the CPAMs formed enantiotropic cholesteric phases, whereas PAM with $DE{\geq}2.2$ exhibited monotropic cholesteric phases. PAM with $DE{\geq}2.2$ and CPAMs with (6-cholesteryloxycarbonyl)pentanoyl DE (DS) more than 1.0 formed cholesteric phases with left-handed helical structures whose optical pitches (${\lambda}_{m'}s$) decrease with increasing temperature. However, the ${\lambda}_{m'}s$ of these samples decreased with increasing DS at the same temperature. On the other hand, CAMs, PAM with DE=1.6, and CPAM with DS=0.3 did not display reflection colors over the full cholesteric range, suggesting that the helicoidal twisting power of the cholesteryl group highly depends on the length of the spacer joining the cholesteryl group to the main chain and DS. The thermal stability and degree of order in the mesophase observed for the amylose derivatives highly depended on DE or DS. The results were discussed in terms of the difference ul the hydrogen bond, the internal plasticization, and the decoupling of the motion of side group with the main chain.

The Effect of Teamwork by Servant Leadership on Job Satisfaction (서번트 리더십에 따른 팀워크가 직무 만족에 미치는 영향)

  • Ko, Ki-Chul;Kim, Gyeong-Hwan;Cho, Yong-Bum
    • Culinary science and hospitality research
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    • v.16 no.5
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    • pp.149-163
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    • 2010
  • This study planned study model through documentary research and drew hypothesis to analyze how teamwork of servant leadership has an influence on job satisfaction aiming at full time employee under department head and contract worker of five star hotel in Busan. As a survey term, from April 5th, 2010 to April 23th, 2010, it distributed total 300 questionnaires, 60 questionnaires for each hotel, and then 242 questionnaires were used for empirical analysis. It analyzed data using SPSS WIN Version 12.0 statistics package program, and verified through frequency analysis, reliability analysis, factor analysis, regression analysis. As a result of verification for influence of servant leadership on interchange which is one of teamwork, it showed that community formation factor has a positive influence on interchange, and as a result of verification for influence on communication which is one of teamwork, I could find out that higher the level of bond of sympathy formation and community formation factor are, higher the communication becomes. As a result of verification for influence on interdependence which is one of teamwork, it showed that community formation, stewardship, vision, commitment to growth factor have a positive influence on interdependence. As a result of verification for influence of teamwork factor on job satisfaction, it showed that all factors have a positive influence. As a result of this study. I could find out that member' positive perception on leader in hotel organization can be a basis deriving job satisfaction though teamwork.

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Seismic Performance Analysis of RC Piers with Lap-spliced Reinforced Steel and Differentiated Aspect Ratios (주철근 겹침이음 및 형상비에 따른 철근콘크리트 교각의 내진거동 분석)

  • Cho, Chang-Beck;Shin, Ho-Jin;Kwahk, Im-Jong;Chung, Young-Soo
    • Journal of the Earthquake Engineering Society of Korea
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    • v.16 no.5
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    • pp.41-53
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    • 2012
  • The objective of this study is to evaluate the seismic capacity of RC piers with small aspect ratios. Test specimens were selected from the prototype piers among existing national roadway bridges which are expected to fail in shear and/or complex shear-flexural mode. Two groups of full scale RC pier models were constructed with aspect ratios of 2.25 and 2.67. Quasi-static tests have been implemented to investigate the failure behavior of the RC piers in terms of the lap-spliced longitudinal reinforcing steel and the aspect ratio. It is confirmed that regarding its shear-flexural behavior, the pier is very sensitive to the aspect ratio or details. In the case of a test pier with highly lap-spliced longitudinal bars, the bond failure of lap-splice steels was the dominant cause of failure before the occurrence of flexure or shear-flexural failure, despite a slight change in the aspect ratio. Finally, based on the test results and analysis, this paper proposes formulas for the yielding and ultimate displacements of circular reinforced concrete bridge piers without seismic details. These formulas will be useful for the investigation and upgrade of the seismic capacity of bridge piers without seismic details.