• Proceedings of the Plant Resources Society of Korea Conference
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• 2019.04a
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• pp.43-43
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• 2019

Deparia pycnosora (Christ) M. Kato is a fern used as ornamental plant. In addition, it is called "Teol-go-sa-ri" in Korean name. The aim of this study was to develop a practical propagation method of D. pycnosora using tissue culture technique. Prothallus obtained from spore germination was the used as experiment materials. The prothalli (300 mg) used in all experiments were sub-cultured for 8-week intervals. The most suitable media for prothallus propagation were identified by culturing 300 mg of prothalli in $1/4{\times}$, $1/2{\times}$, $1{\times}$, $2{\times}$ MS medium and in Knop medium for 8 weeks. Also, the prothalli were cultured by chopping with a scalpel. In addition, sucrose, activated charcoal, and total nitrogen source were added in different concentrations based on the culture medium selected. Cultures were maintained at a temperature of $25{\pm}1^{\circ}C$, light intensity of $30{\times}1.0{\mu}mol-m-2{\cdot}s-1$, and a photoperiod of 16/8 h (light/dark) in in vitro. The results showed that optimum was achieved prothallus fresh weight and development in $1{\times}$ MS medium. When other components were added to the basic $1{\times}$ MS medium, prothallus propagation was maximized in $1{\times}$ MS medium supplemented with 2% sucrose, 0.2% activated charcoal, and 60 mM total nitrogen. To select a suitable soil mixture for sporophyte formation, 1.0 g of prothallus was blended with distilled water, spread on five combinations of different soil substrates (decomposed granite, horticultural substrates, peat moss, and perlite), and cultivated for 12 weeks. The sporophyte cultures were maintained at a temperature of $25{\pm}1^{\circ}C$, light intensity of $43{\pm}2.0{\mu}mol-m-2{\cdot}s-1$, humidity of $84{\pm}1.4%$, and a photoperiod of 16/8 h (light/dark). As a results, horticultural substrate alone, 2:1 (v:v) mixtures of horticultural substrate and perlite, and 2:1 mixtures of horticultural substrate and decomposed granite induced 208.0, 201.3 and 248.8 sporophytes per pot, respectively. Therefore, this result could provide a practical mass propagation method of D. pycnosora

• Journal of Animal Science and Technology
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• v.61 no.1
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• pp.1-9
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• 2019

Little is known about the effects of the plane of nutrition on growth performance and meat quality of grow-finish pigs under commercial production conditions. The present study was thus addressed to this virtually unanswered question. One hundred and two barrows and 102 gilts weighing approximately 24 kg were fed phase I and II grower diets with a high, medium, or low plane of nutrition (HP, MP, or LP) to approximately 43 and 70 kg, respectively, in 6 replicates (pens). Subsequently, the HP and MP groups were fed the HP and MP1 finisher diets, respectively, the LP group being fed a second MP (MP2) finisher diet (LP1 group). Moreover, 68 LP-grower-fed barrows and gilts were added to the feeding trial and fed the MP1 and LP finisher diets to approximately 95 kg and thereafter, respectively (LP2 group). All MP diets had the lysine:calorie ratios comparable to the RNC recommendations, with < 18% differences between those of the HP and LP diets. The finisher pigs were reared in 16 pens and slaughtered at approximately 115 kg. The gain:feed ratio, but not average daily gain (ADG), was greater for the HP group than for the MP and LP during the grower phase I whereas during the grower phase II, ADG was greater (p < 0.05) for the HP and LP groups vs. MP. During the finisher phase I, ADG was less for the LP (LP1 + LP2) group vs. HP and MP, with no difference between the HP and MP groups; the gain:feed ratio was less for the LP vs. MP group. Backfat thickness was greater for the LP vs. HP group. The water holding capacity of fresh longissimus dorsi muscle (LM) and the sensory juiciness score for cooked LM were greatest for the LP group, the sensory flavor and tenderness scores being greater for the LP group vs. MP. In conclusion, results suggest that compensatory growth occurred for the LP and MP groups during the grower phase II and finisher phase I, respectively, with fat deposition increased for the LP group and that meat quality could be improved by the use of LP.

• Journal of the Korea institute for structural maintenance and inspection
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• v.23 no.4
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• pp.113-119
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• 2019

Many researches on alternative materials as construction materials is continuing by recycling industrial byproducts due to shortage of sitereclamation and natural aggregates. In this paper, engineering properties in early-aged OPC (Ordinary Portland Cement) and GGBFS (Ground Granulated Blast Furnace Slag) concrete are evaluated with EOS aggregate replacement. The related experiments were carried out with 0.6 of water to binder ratio, three levels of EOS replacement ratios (0%, 30% and 50%) for fine aggregate, and two levels of cement replacement with GGBFS (0% and 40%). Several tests such as slump air content, and unit mass measurement are performed for fresh concrete, and compressive strength and diffusion coefficient referred to NT BUILD 492 method are measured for hardened concrete. Through the tests, it was evaluated that the compressive strength in concrete with EOS aggregate increased to 3 days and 7 days but slightly decreased at the age of 28 days. In the accelerated chloride penetration test, GGBFS concrete showed reduced diffusion coefficients by 60 - 67% compared with OPC concrete. The lowest chloride diffusion coefficient was evaluated in the 50% replacement with EOS aggregate, which showed an applicability of EOS aggregate to concrete production.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.55 no.3
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• pp.217-232
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• 2019

It was turned out by shrimp beam trawl monthly survey from March, 2015 to February, 2016 that different species composition and abundance of the fish assemblages in Sacheon Bay and coastal waters off Namhae, Korea were compared. As a result of monthly measured sea temperature and salinity of Sacheon Bay and coastal waters off Namhae, sea temperature of both areas was changed seasonally; however, differences in sea temperature occurred during certain periods depending on the region. Salinity was generally low in Sacheon Bay affected by fresh water, and both areas was low in summer and high in winter. A total of 73 species representing 37 families were collected in Sacheon Bay. The dominant fish species in terms of numbers and biomass were Liparis tanakae, $23,077inds./km^2$, $332.1kg/km^2$. A total of 91 fish species representing 49 families were collected in coastal waters off Namhae. The dominant fish species in terms of numbers were Leiognathus nuchalis, $139,683inds./km^2$ and biomass were Chelidonichthys spinosus, $1,078.6kg/km^2$. Analysis of dendrogram of the clustering showed that Sacheon Bay and coastal waters off Namhae were distinctive featured (global R = 0.691, p = 0.017). And except of summer season (July-October), there was a distinctive feature seasonally (global R = 0.844, p = 0.001). The fish species that appeared in common in both areas, where fish species caught in Sacheon Bay, an important inner bay,were smaller than those caught in coastal waters off Namhae appeared. It presented that Sacheon Bay plays a more important role in spawning and nursery ground for fisheries resource than coastal waters off Namhae, Korea.

• Research in Plant Disease
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• v.25 no.3
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• pp.136-142
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• 2019

Drought stress is considered as one of major abiotic stresses; it leads to reduce plant growth and crop productivity. In this study, we selected bacterial strains for alleviating drought stress in chili pepper plants. As drought-tolerant bacteria, 28 among 447 strains were pre-selected by in vitro assays including growth in drought condition with polyethylene glycol and plant growth-promoting traits including production of 1-aminocyclopropane-1-carboxylate deaminase, indole-3-acetic acid and exopolysaccharide. Sequentially, 7 among pre-selected 28 strains were screened based on relative water content (RWC); GLC02 and KJ40, among seven strains were finally selected by RWC and malondialdehyde (MDA) in planta trials under an artificial drought condition by polyethylene glycol solution. Two strains GLC02 and KJ40 reduced drought stress in a natural drought condition as well as an artificial condition. Strains GLC02 or KJ40 increased shoot fresh weight, chlorophyll and stomatal conductance while they decreased MDA in chili pepper plants under a natural drought condition. However, two strains did not show biocontrol activity against diseases caused by Phytophthora capsici and Xanthomonas campestris pv. vesicatoria in chili pepper plants. Taken together, strains GLC02 or KJ40 can be used as bio-fertilizer for alleviation of drought stress in chili pepper plants.

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.10
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• pp.1528-1539
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• 2019

Objective: To evaluate the effects on microbial diversity and biochemical parameters of gradually increasing temperatures, from $5^{\circ}C$ to $25^{\circ}C$ on corn silage which was previously fermented at ambient or low temperature. Methods: Whole-plant corn silage was fermented in vacuum bag mini-silos at either $10^{\circ}C$ or $20^{\circ}C$ for two months and stored at $5^{\circ}C$ for two months. The mini-silos were then subjected to additional incubation from $5^{\circ}C$ to $25^{\circ}C$ in $5^{\circ}C$ increments. Bacterial and fungal diversity was assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) profiling and biochemical analysis from mini-silos collected at each temperature. Results: A temperature of $10^{\circ}C$ during fermentation restricted silage fermentation compared to fermentation temperature of $20^{\circ}C$. As storage temperature increased from $5^{\circ}C$ to $25^{\circ}C$, little changes occurred in silages fermented at $20^{\circ}C$, in terms of most biochemical parameters as well as bacterial and fungal populations. However, a high number of enterobacteria and yeasts (4 to $5\;log_{10}$ colony forming unit/g fresh materials) were detected at $15^{\circ}C$ and above. PCR-DGGE profile showed that Candida humilis predominated the fungi flora. For silage fermented at $10^{\circ}C$, no significant changes were observed in most silage characteristics when temperature was increased from $5^{\circ}C$ to $20^{\circ}C$. However, above $20^{\circ}C$, silage fermentation resumed as observed from the significantly increased number of lactic acid bacteria colonies, acetic acid content, and the rapid decline in pH and water-soluble carbohydrates concentration. DGGE results showed that Lactobacillus buchneri started to dominate the bacterial flora as temperature increased from $20^{\circ}C$ to $25^{\circ}C$. Conclusion: Temperature during fermentation as well as temperature during storage modulates microorganism population development and fermentation patterns. Silage fermented at $20^{\circ}C$ indicated that these silages should have lower aerobic stability at opening because of better survival of yeasts and enterobacteria.

• Journal of Nutrition and Health
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• v.54 no.5
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• pp.448-458
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• 2021

Purpose: Mulberry (Morus alba L.) fruit is widely grown in Asia and consumed as fresh fruit, jam, and juices. The fruit has beneficial health effects, including anti-diabetic, anti-tumor, and anti-obesity properties. However, the mechanisms by which mulberry fruit juice powder (MJ) regulates inflammatory microRNAs (miRs) are not yet known. This study investigated the effect of mulberry fruit juice powder on the regulation of inflammation and miR-132/143 during 3T3-L1 adipocyte differentiation. Methods: The 3T3-L1 cells were induced to differentiate for 2 days and then treated with various concentrations of MJ for 7 days. Cytotoxicity was determined by evaluating cell viability using a water-soluble tetrazolium salt-8 assay kit. Intracellular lipid accumulation was evaluated by oil-red O staining. The levels of the expression of genes involved in adipogenesis and inflammation, and miR-132/143 were measured by quantitative real-time polymerase chain reactions. Results: MJ showed no cytotoxic effect on 3T3-L1 adipocytes at concentrations below 100 ng/mL. Intracellular lipid accumulation was reduced by MJ treatment at concentrations of 100 ng/mL. The messenger RNA (mRNA) levels of proliferator-activated receptor-γ, cytosine-cytosine-adenosine-adenosine-thymidine/enhancer-binding protein-α, and adipocyte protein 2, which are involved in adipogenesis, were suppressed by MJ. A reduction was also seen in mRNA levels of genes related to the inflammatory response, such as tumor necrosis factor-α, interleukin-6, monocyte chemoattractant protein-1, and inducible nitric oxide synthase. The expression of the inflammatory miR-132 and miR-143 was also decreased by MJ. Conclusion: These results suggest that MJ may suppress adipogenesis and inflammation through the regulation of miR-132/143 expression in 3T3-L1 adipocytes. Thus, MJ may be useful as a food agent that prevents obesity-associated inflammation.

• Journal of Plant Biotechnology
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• v.48 no.2
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• pp.77-85
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• 2021

Hydroponic cultivation, in which crops are grown without soil and are unaffected by the weather, has many advantages over conventional soil cultivation. The crop's growth can be further accelerated by using nutrient solution in place of water. This study investigated the growth and physicochemical properties of hydroponic barley sprouts under various nutrient solution and artificial light treatments. The shoot, root, and total plant length increased over time, with the fastest growth occurring in the nutrient solution and light-emitting diode (LED) treatments. Fresh and dry plant weights were higher in the fluorescent lamp treatment than in the LED treatment. Barley sprout powder color differed slightly by treatment, with the Hunters L value ranging from 50.79 to 53.77; Hunters a value from -6.70 to -4.42; and Hunters b value from 13.35 to 14.76. The Hunters L and Hunters b values were highest in the LED treatment, whereas the Hunters a value was relatively highest in the fluorescent lamp treatment. The total phenol content was higher in the control than in the nutrient solution treatment; however, the total flavonoid content showed the opposite pattern to that of total phenol content, being highest in plants that were grown in nutrient solution. The Trolox equivalent antioxidant capacity (TEAC) was higher in the control group than in the nutrient solution group. The ferric ion reducing antioxidant power (FRAP) was higher in the fluorescent treatment group than in the LED treatment group. The total amino acid composition ranged from 106.82 to 122.63 mg/g dry powder, with the essential amino acid composition ranging from 47.01 to 56.19 mg/g, and non-essential amino acid composition from 67.86 to 77.66 mg/g. The most frequently detected compositional amino acid was aspartic acid, followed by glutamic acid, alanine, leucine, and valine.

• Journal of Animal Science and Technology
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• v.63 no.1
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• pp.91-103
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• 2021

To improve the fermentation quality of silage and reduce the nutrients loss of raw materials during the ensiling process, silage additives are widely used. The effect of additives on silage is also affected by the species of crop. Therefore, this study was designed to explore the effects of formic acid (FA) and lactic acid bacterial inoculant on the quality of main summer crop silage. The experiment was consisted on split-plot design with three replications. The experiment used the main summer forage crops of proso millet ("Geumsilchal"), silage corn ("Gwangpyeongok"), and a sorghum-sudangrass hybrid ("Turbo-gold"). Treatments included silage with Lactic acid bacterial Inoculant (Lactobacillus plantarum [LP], 1.0 × 106 CFU/g fresh matter), with FA (98%, 5 mL/kg), and a control (C, without additive). All silages were stored for 60 days after preparation. All additives significantly increased the crude protein content and in vitro dry matter digestibility (IVDMD) of the silages and also reduced the content of ammonia nitrogen (NH3-N) and pH. Corn had the highest content of IVDMD, total digestible nutrients and relative feed value among silages. Compared with the control, irrespective of whether FA or LP was added, the water soluble carbohydrate (WSC) of three crops was largely preserved and the WSC content in the proso millet treated with FA was the highest. The treatment of LP significantly increased the lactic acid content of the all silage, while the use of FA significantly increased the content of acetic acid (p < 0.05). The highest count of lactic acid bacteria (LAB) was detected in the LP treatment of corn. In all FA treatment groups, the total microorganism and mold numbers were significantly lower than those of the control and LP groups (p < 0.05). In conclusion, both additives improved the fermentation quality and nutritional composition of the main summer forage crops. The application of FA effectively inhibited the fermentation of the three crops, whereas LAB promoted fermentation. So, both FA and LP can improve the quality of various species of silage.

• Journal of the Korea institute for structural maintenance and inspection
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• v.24 no.6
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• pp.180-188
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• 2020

To replace Japanese coal ash used in the domestic cement production and to recycle large quantities of domestic pond ash, it is essential to develop the technologies for quality control of cement by using the domestic pond ash. Thus, in this study, the feasibility of using the pond ash as a raw material for cement was investigated through chemical composition and microstructure analysis. As a result, most of the domestic pond ash contained slightly more Fe2O3, chloride, and unburned carbon contents than Japanese coal ash. In particular, the contents of chloride were considerably low in the pond ash that was transferred to fresh water or collected from surface of landfill area. However, since circulating fluidized bed boiler coal ash had relatively high SO3 contents causing durability problems of cement, it was not suitable for use as a raw material for cement. Thus, to replace Japanese coal ash with the domestic pond ash, it is necessary to introduce the adjustment of mixture proportion of cement raw materials and the process of removing chloride in the pond ash.