• 한국동물위생학회지
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• 제35권3호
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• pp.223-230
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• 2012

This study was carried out to investigate the heavy metal and microbiological hazards on by-products (liver, omasum, small intestines) of cattle. From April to October in 2011, one hundred and twenty samples were equally collected from slaughterhouse and meat by-product markets in Incheon city. The total bacteria counts and E. coli count were applied to assess the microbiological quality. Food borne bacteria including Salmonella spp., Listeria monocytogenes, Staphylococcus aureus, Clostridium perfringens and E. coli O157:H7 were also determined. The results were obtained as follows: The undesirable grade (more than $10^5\;CFU/cm^2$) was detected in the by-product from 18.3% (slaughterhouse) and 23.3% (by-product markets). The frequency of generic E. coli (more than $10^2\;CFU/cm^2$) from the slaughterhouse was 20.0%, whereas that of the meat by-product markets was 26.7%. Of the samples from slaughterhouse, 3 (5.0%), 5 (8.3%), and 12 (20.0%) samples were contaminated with Salmonella spp, S. aureus, and C. perfringens, respectively. S. aureus and C. perfringens were also detected in 6 (10.0%) and 25 (41.7%) samples in the meat by-product markets, respectively. Nine of 11 S. aureus isolates harbored toxin gene. However, the cpe gene of C. perfringens was not detected among the 37 isolates. The detection rate was higher in August than in February, April and June. The levels of Cd and Pb in all the samples tended to be low (<0.2 mg/kg). This preliminary data could be used for legislation on the regulation and control of microorganism and heavy metal in by-products of cattle.

• 약학회지
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• 제52권2호
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• pp.137-146
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• 2008

Even though driving under the influence of drug (DUID) is a worldwide problem, we, Korea has no regulation system yet except for alcohol, and there are little cases reported related to DUID. In order to investigate the type of abused drugs for drivers in Korea, we tried to analyze controlled and non-controlled drugs in alcohol-positive blood samples. 275 whole bloods, which were positive for alcohol on the roadside test, were collected from the police for two months ($Nov.{\sim}Dec.$ 2006). The analytical strategy was constituted of three steps: First, alcohol in blood samples were confirmed and quantified by gas chromatography. Second, controlled drugs were screened by $Evidence_{investigator}\;^{TM}$ (Randox, U.K.) as preliminary test. It was based on immunoassay by biochip array analyzer. Nine groups of drug abuse were screened: amphetamines, methamphetamines, cannabis, cocaine, opiates, barbiturates, methadone, benzodiazepines I (oxazepam) & II (lorazepam). Finally, confirmation of these drugs was performed by GC-MS. Blood samples were extracted by solid-phase extraction by $RapidTrace^{TM}$ (Zymark, U.S.A.). After trimethylsilyl (TMS) derivatization, eluates were analyzed to GC-MS. Total 49 drugs were investigated in this study including controlled drugs, antidepressants, 1st generation antihistamines, dextromethorphan, nalbuphine, ketamine, etc. For rapid detection, we developed the automated identification system. It was made up a new software, "DrugMan", modified Chemstation data analysis menu and newly developed macro modules. A series of peak selection, identification and reporting of the results were performed automatically by this system. Concentrations of alcohol in 275 blood samples were ranged from 0.011 to 0.249% (average, 0.119%). Among 149 blood samples, just six samples (4.0%) were showed positive results to the immunoassay: one methamphetamine and five benzodiazepines group I. By GC-MS confirmation, only benzodiazepines were detected and methamphetamine was not detected from immunoassay positive blood sample. Besides these drugs, 5 chlorpheniramines, dextromethorphan, diazepam, doxylamine, ibuprofen, lidocaine and topiramate were also detected in whole bloods by GC-MS. Conclusively, the frequency of drug abuse for Korean drivers was relatively low. There was none case which illegal drug was detected. However these results were limited to alcohol positive blood samples, so it is necessary to analyze more samples including alcohol negative blood.

• International Journal of Oral Biology
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• 제31권4호
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• pp.127-133
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• 2006

[ $H_2O_2$ ], a member of reactive oxygen species (ROS), is known to be involved in the mediation of physiological functions in a variety of cell types. However, little has been known about the physiological role of $H_2O_2$ in exocrine cells. Therefore, in the present study, the effect of $H_2O_2$ on cholecystokinin (CCK)-evoked $Ca^{2+}$ mobilization and amylase release was investigated in rat pancreatic acinar cells. Stimulation of the acinar cells with sulfated octapeptide form of CCK (CCK-8S) induced biphasic increase in amylase release. Addition of $30\;{\mu}M\;H_2O_2$ enhanced amylase release caused by 10 pM CCK-8S, but inhibited the amylase release induced by CCK-8S at concentrations higher than 100 pM. An ROS scavenger, $10\;{\mu}M$ Mn(III)tetrakis(4-benzoic acid)porphyrin chloride, increased amylase release caused by CCK-8S at concentrations higher than 100 pM, although lower concentrations of CCK-8S-induced amylase release was not affected. To examine whether the effect of $H_2O_2$ on CCK-8S-induced amylase release was exerted via modulation of intracellular $Ca^{2+}$ signaling, we measured the changes in intracellular $Ca^{2+}$ concentration $([Ca^{2+}]_i)$ in fura-2 loaded acinar cells. Although $30\;{\mu}M\;H_2O_2$ did not induce any increase in $[Ca^{2+}]_i$ by itself, it increased the frequency and amplitude of $[Ca^{2+}]_i$ oscillations caused by 10 pM CCK-8S. However, $30\;{\mu}M\;H_2O_2$ had little effect on 1 nM CCK-8S-induced increase in $[Ca^{2+}]_i$. ROS scavenger, 1 mM N-acetylcysteine, did not affect $[Ca^{2+}]_i$ changes induced by 10 pM or 1 nM CCK-8S. Therefore, it was concluded that $30\;{\mu}M\;H_2O_2$ enhanced low concentration of CCK-8S-induced amylase release probably by increasing $[Ca^{2+}]_i$ oscillations while it inhibited high concentration of CCK-8S-induced amylase release.

• 조명전기설비학회논문지
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• 제21권6호
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• pp.117-125
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• 2007

연료전지 발전시스템에서는 DC-DC 승압용 컨버터와 DC-AC 인버터가 필요하다. 그러므로 본 논문에서는 연료전지의 전압을 380[VDC]로 승압하기 위한 절연형 DC-DC 컨버터와 단상 220[VAC]로 변환하기 위한 LC필터를 가진 PWM 인버터로 구성된 전력변환장치를 제안하였다. 특히 제안한 연료전지 시스템용 고효율 DC-DC 컨버터는 위상천이 PWM 제어법을 이용하여 부분 공진에 의한 ZVS를 실현하였으며, 일정 스위칭 주파수화 및 스위치의 스위칭 손실, 피크 전압과 전류를 저감시켰다. 그리고, 정류회로에 2개의 인덕터를 첨가하여 리플성분이 저감된 직류전압과 전류를 부하측에 안정하게 공급할 수 있었다. 또한, 넓은 출력 전압 조정에도 효율을 92[%]정도 얻을 수 있다. 이상과 같이 결과는 시뮬레이션과 실험을 통하여 그 타당성을 확인하였다.

• 한국컴퓨터정보학회논문지
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• 제25권2호
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• pp.197-203
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• 2020

본 연구의 목적은 항공사 승무원 취업준비생들의 항공사 객실승무원 이미지메이킹 규정변화에 대한 인식을 알아보고, 규정변화인식, 직업선택동기 및 진로결정 간의 영향관계를 규명하는데 있다. 이 연구에서는 광주, 전남 지역 항공서비스전공 여대생들을 대상으로 설문조사를 실시하였으며, 2019년 11월 18일부터 12월 13일까지 진행하였다. 총 203부의 설문지를 실증분석에 이용하였다. 수집된 자료는 'SPSS statistics 21.0'을 이용하여 빈도분석, 요인분석, 신뢰도분석, 회귀분석을 하였다. 연구결과 항공서비스전공 학생들은 객실승무원 이미지메이킹 규정변화에 대해 긍정적으로 인식하고 있으며, 규정변화에 대한 인식은 직업선택동기와 진로결정에 유의한 영향을 미치고, 직업선택동기는 진로결정에 유의한 영향을 준다고 나타났다. 결론적으로 항공사에는 채용할 인재를 이해할 수 있는 정보를, 취업준비생에게는 취업을 위한 정보를 제공한다는 시사점을 갖는다. 그러나 이 연구의 표본대상의 지역적 한계, 성별적 한계와 표본 크기의 한계점을 갖는다.

• Molecules and Cells
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• 제39권6호
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• pp.468-476
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• 2016

PLZF-expressing invariant natural killer T cells and CD4 T cells are unique subsets of innate T cells. Both are selected via thymocyte-thymocyte interaction, and they contribute to the generation of activated/memory-like CD4 and CD8 T cells in the thymus via the production of IL-4. Here, we investigated whether $PLZF^+$ innate T cells also affect the development and function of $Foxp3^+$ regulatory CD4 T cells. Flow cytometry analysis of the thymus and spleen from both CIITA transgenic C57BL/6 and wild-type BALB/c mice, which have abundant $PLZF^+$ CD4 T cells and invariant natural killer T cells, respectively, revealed that $Foxp3^+$ T cells in these mice exhibited a $CD103^+$ activated/memorylike phenotype. The frequency of $CD103^+$ regulatory T cells was considerably decreased in $PLZF^+$ cell-deficient $CIITA^{Tg}Plzf^{lu/lu}$ and $BALB/c.CD1d^{-/-}$ mice as well as in an IL-4-deficient background, such as in $CIITA^{Tg}IL-4^{-/-}$ and $BALB/c.IL-4^{-/-}$ mice, indicating that the acquisition of an activated/ memory-like phenotype was dependent on $PLZF^+$ innate T cells and IL-4. Using fetal thymic organ culture, we further demonstrated that IL-4 in concert with TGF-${\beta}$ enhanced the acquisition of the activated/memory-like phenotype of regulatory T cells. In functional aspects, the activated/ memory-like phenotype of Treg cells was directly related to their suppressive function; regulatory T cells of $CIITA^{Tg}PIV^{-/-}$ mice more efficiently suppressed ovalbumin-induced allergic airway inflammation compared with their counterparts from wild-type mice. All of these findings suggest that $PLZF^+$ innate T cells also augmented the generation of activated/memory-like regulation via IL-4 production.

• 지역사회간호학회지
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• 제11권2호
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• pp.538-555
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• 2000

The purpose of this study, in which 573 students from 2 high schools in Kang Nung city participated was to identify the attitude and experience of sexual harassment. This information will provide useful data, and promote a more systematic sexual harassment education program. The subjects of this study were 278 male students and 295 female students. The data was collected from September 25 to October 6. 2000. The data was analyzed using the statistical Computer package. SPSS to manipulate the data along with frequency, percentage, and mean t-test. The results from this study were summarized as follows. 1. The results from this comparison between two groups were significant in their attitude on sexual harassment(t=-2.26. p=0.024). Female students had higher scores than male students. 2. As a cause of sexual harassment. 'A man's misjudgement regarding a women as sexual objects(n=130: 22.7%)'. 'Patriarchal system of unequal distribution of power and status between the sexes(n=105: 18.3%)'. 'The effect of sexual stimulatives. (pornography. pornovideo. TV program ect.)(n=89: 15.5%)'. 3. As a countermeasure for prevention, 'Punishment, law and regulation of the sexual harasser(n = 151; 26.4%)', 'A preventive education of sexual harassment in adolescence (n = 125: 21.8%)'. 'Reformation of gender inequality and sex discrimination in the socio-structure(n=76; 13.3%)'. 4. Out of 573 students who participated in this study, 209(36.5%) students reported experiencing an incident of sexual harassment. For 278 males who participated in this study, 88(31.7%) had experienced an incident of sexual harassment. For the 295 females who participated in this study, 121(41.0%) had experienced an incident of sexual harassment. The type of sexual harassment is reported physical sexual harassment(n = 248: 40.5%), verbal sexual harassment(n = 226; 36.7%), and visual sexual harassment(n=139: 22.6%). 5. Their age in which they had experienced harassment was usually 'after 16 years of age(n=122: 58.4%)'. The age of harassers were mostly teenagers(n=112; 53.6%). The relationship to the with sexual harasser was a stranger (n=85; 40.7%), or a friend(n=78: 37.3%). After the incident of sexual harassment occurred, some students informed friends (n=114: 54.5%), 63(30.1%) told no one. Male students' feelings after the experienced harassment ranged from: 'be interesting', 'be pleasant'. Female students' feelings after the experienced harassment ranged from; 'be surprised', 'get a shock', 'fear', 'shame'.

• 한국전자파학회논문지
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• 제23권7호
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• pp.805-814
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• 2012

무선기기의 간헐적인 신호 또는 높고 낮은 진폭의 주기적인 신호 전송은 오디오 주파수에 간섭 신호를 발생시켜 근접 거리에 설치된 음향 기기에 잡음을 발생시킨다. 최근 이와 관련하여 Global system for Mobile Communication(GSM) 폰과 유사하게 간헐적인 신호 전송을 하는 Long Term Evolution(LTE) 폰에 의한 오디오 잡음현상이 보고되었다. 현재 국내 KN20 규격에서는 잡음에 대한 내성 능력을 확보하기 위하여 음향 및 TV 방송수신기의 내성 시험 기준 신호로 변조 주파수 1 kHz Amplitude Modulation(AM) 80 %와 Code Division Multiple Access(CDMA) 신호를 이용하고 있다. 본 논문에서는 LTE 폰의 출시에 따라 기존의 내성 기준 신호 AM과 CDMA를 GSM과 LTE 폰이 발생시키는 오디오 잡음의 수준과 비교한 후, 국내 음향 기기에 대한 내성 기준 신호의 개선을 제안한다. CDMA 신호는 음향 기기에 간섭을 거의 일으키지 않는 것으로 여겨지므로 시험 항목에서 삭제해도 무방할 것으로 판단된다. 반면, LTE는 많은 사용자가 이용하고 있으며, 음향 기기 등에 무시할 수 없는 간섭을 발생시킬 것으로 분석되므로 CDMA 신호를 대신하여 음향 기기 및 TV 방송 수신기의 내성 시험을 위한 시험 기준 신호로 규정하는 것이 바람직할 것으로 사료된다.

• 한국통신학회논문지
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• 제32권1A호
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• pp.83-90
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• 2007

본 논문에서는 ISO/IEC 18000-6C 표준안을 만족하는 UHF대역 RFID 수신단(리더)의 지터(Jitter)처리와 글리치제거 알고리듬 및 설계방안을 제안하고 이를 이용한 리더를 구현하여 실제 TI(Texas instrument) Gen2 태그의 응답을 분석하였다. ISO/IEC 18000-6C표준안은 Reader에서 Tag로 데이터 전송 시 +/-1%의 오차와 Tag에서 Reader로 데이터 전송 시 최대 +/-22%의 오차를 허용하도록 정의하고 있다. 이러한 허용오차범위 내의 데이터에 대해 본 논문에서 제시한 회로는 기존의 PLL(DPLL, ADPLL)을 이용한 방식이 아닌 최대허용치(tolerance)와 허용치누적을 이용하여 일정치의 오차범위를 허용하며 디코딩 하도록 설계하였다. 또한 글리치와 지터제거 알고리듬의 기본원리를 동일하게 구성하여 글리치제거와 지터제거를 따로 구분하지 않고 하나의 기능으로 동작하게 한다. 주 클럭은 19.2MHz로 설정하였으며 LF는 국내 전파법에 맞도록 40kHz로 설정하였다 시뮬레이션결과 15%이하의 위상지터를 가진 입력데이터에 대해 판독에러율은 0이었으며 $15%{\sim}22%$ 위상지터를 가진 입력데이터에 대해서 는 0.000589였다. 그러나 동적LF생성회로를 사용한 결과 $15%{\sim}22%$ 위상변화를 가진 입력데이터에 대해 판독에러율은 0이었으며 표준안에 정의된 최대 +/-22%오차 범위내의 지터 발생에 대해서 판독에러율은 0이었다.

• 정보관리연구
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• 제40권1호
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• pp.69-86
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• 2009

최근 정보통신 분야의 가장 큰 패러다임은 유비쿼터스 컴퓨팅(ubiquitous computing)이다. 이는 유비쿼터스 사회(ubiquitous society)를 실현하는 기반 기술로서 사회, 경제, 문화 등 모든 분야에 긍정적인 영향력을 미치게 될 것이나, 유비쿼터스 사회로의 진입은 개인 프라이버시를 침해할 수 있다는 우려의 목소리도 함께 나타나고 있다. 따라서 이를 보호하기 위한 법적, 기술적 규제 방안의 존재는 향후 RFID 기술 및 산업의 확산에 큰 장애요인이 될 것이며, 문제 해결을 위해 기술적인 방법으로만 접근한다면 기업 측면에서는 막대한 비용과 기술력을 투자해야만 할 것이다. 이에 본 연구에서는 한필구(2006)가 개발한 "RFID 활용단계별 개인정보보호 영향평가(안)"의 8개의 RFID 활용단계, 단계별 과정, 점검사항 그리고 85개의 평가항목들을 RFID 개인정보 영향평가를 측정할 수 있는 지표로 선정하여, 해당전문가를 대상으로 설문조사를 통한 객관적 자료를 활용해 RFID 개인정보 영향평가지수를 개발하고자 한다. 이는 RFID 관련 분야 기업에는 타당성과 신뢰성 있는 자료를 제공할 수 있을 것이며, RFID 개인정보에 관한 정책 수립에도 활용할 수 있을 것이다. 또한 RFID 활용에 있어서 개인정보에 대한 진단 도구로서 하나의 기준 및 유비쿼터스 사회의 기반 구축에 기여할 것으로 기대한다.