• Title/Summary/Keyword: freezing condition

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Flowering Characteristics and Optimal Harvest Time in Wasabia japonica Mastum (고추냉이의 개화(開花) 특성(特性) 및 채종적기(採種適期))

  • Lee, Sung-Woo;Park, Chang-Hwan;Kim, Suk-Dong;Choi, Keong-Gu
    • Korean Journal of Medicinal Crop Science
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    • v.6 no.3
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    • pp.227-231
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    • 1998
  • Flowering characteristics and optimal harvest time for seed of wasabi variety. Daruma, were investigated from Dec. 1995 to June 1996 in film house of Suwon. The number of peduncle was $10.2{\pm}2.29$ and most of the peduncle emerged between Jan. 20 and Feb. 10. Average peduncle length was $124{\pm}29.60cm$ and the peduncle grew most rapidly in the middle of march. Flowering started on Jan. 27 and lasted until June 4 with a peak at march 21 and flowering period was $99{\pm}21.32$ days. Flowers which bloomed later than the middle of March set seed but those bloomed earlier became sterile because days with minimum temperature below freezing occurred until the middle of March. Maturing period was $37.5{\pm}2.60$ days and total seed set was $43.2{\pm}8.77%$ in the natural culture condition. Optimal harvest time for seed was the last ten days of May and seed yield per $m^2$ was $79.0{\pm}19.94g%$(11,177 seeds).

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Propagation of asymbiotically germinated seedlings with liquid culture for endangered lady's slipper orchid (Cypripedium macranthos Sw.) (액체배양 방법을 이용한 멸종위기종 복주머니란 종자 무균발아 및 증식)

  • Lee, Joung Kwan;Huh, Yoon Sun;Park, Sang Im;Park, Jae Seong;Jeong, Mi Jin;Son, Sung Won;Suh, Gang Uk
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.04a
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    • pp.45-45
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    • 2018
  • We had already reported the successful germination for green pods of purple lady's slipper orchid (Cypripedium macranthos Sw.). The green pod methods is to take immature seeds in green capsules, sterilize the capsule, and take out the sterile seeds. This method, however, needs very critical time of harvest. The critical time of seed harvest changes depending upon the species, condition of the specimen, and climatic influence, and the right time lies between 5 and 12 weeks after fertilization. In this study, the mature seeds were collected after 120-130 days with hand-polination of lady's slipper orchids. Mature seeds are usually dormant and it has to be overcome, either with hormone or storing the seeds near freezing for two or three months to break dormancy. The seeds were first surface sterilized with 70% ethanol and then transferred 1% NaOCl for 10-15 minutes, followed by rinses 3 times with sterilized distilled water. The cypripedium seeds consists of an embryo within a seed coat known as a testa. The testa is water repellent and the seed has a large air space between the embryo and testa so the seed tends to float on water. We had resolved the problems with vacuum pump to soak water into the testa before sterilization. The seeds were placed on liquid or agar solidified germination media. Cultures were incubated at $24{\pm}1^{\circ}C$ in dark. The seeds were germinated in 6-8 weeks in liquid suspension culture (germination percentage over 18%); however, the seeds on agar solidified media took more than 5 months to germinate and the germination percentage less than 5%. The most effective media for liquid culture was 1/4 strength Murashige and Skoog (MS) medium with 50 ml/l coconut water ($4brix^{\circ}$) at pH 5.8.

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Developing an On-Line Monitoring System for a Forest Hydrological Environment - Development of Hardware - (산림수문환경(山林水文環境) 모니터링을 위(爲)한 원거리(遠距離) 자동관측(自動觀測)시스템의 개발(開發) - 하드웨어를 중심(中心)으로 -)

  • Lee, Heon Ho;Suk, Soo Il
    • Journal of Korean Society of Forest Science
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    • v.89 no.3
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    • pp.405-413
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    • 2000
  • This study was conducted to develop an on-line monitoring system for a forest hydrological environment and its meteorological condition, such as temperature, wind direction and speed, rainfall and water level on V-notch, electrical conductivity(EC), potential of hydrogen(PH) by the motor drive sensor unit and measurement with a single-chip microprocessor as controller. These results are summarized as follows ; 1. The monitoring system consists of a signal process unit, motor drive sensor unit, radio modem unit and power supply. 2. The motor drive sensor unit protects the sensor from swift current or freezing and can constantly maintain fixed water level during measurements. 3. This monitoring system can transfer the data by radio modem. Additionally, this system can monitor hydrological conditions in real time. 4. The hardware was made of several modules with an independent CPU. They can be mounted, removed, repaired and added to. Their function can be changed and expanded. 5. These are the result of an accuracy test, the values of temperature, EC and pH measured within an error range of ${\pm}0.2^{\circ}C$, ${\pm}1{\mu}S$ and ${\pm}0.1pH$ respectively. 6. This monitoring system proved to be able to measure various factors for a forest hydrological environment in various experimental stations.

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Evaluation of the Stability of Biodegradable Nanoparticle with Time via Particle Size Measurement (입자 크기 측정을 통한 생분해성 나노입자의 시간에 따른 분산 안정성 평가)

  • Cho, Kuk-Young;Yim, Jin-Heong;Park, Jung-Ki;Lee, Ki-Seok
    • Polymer(Korea)
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    • v.32 no.3
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    • pp.246-250
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    • 2008
  • Colloidal stability of the biodegradable nanoparticle was characterized by measuring the variation of particle size with time using photon correlation spectroscopy. Three kinds of polymers, namely, poly(D,L-lactide-co-glycolide)(PLGA), PLGA/poly(L-lactide) blends, and PLGA/poly(L-lactide)-g-poly(ethylene glycol) blends were used as matrix material for nanoparticle preparation. Nanoparticles were prepared with or without using poly(vinyl alcohol)(PVA) as suspension stabilizer to evaluate the condition of preparation. Nanoparticles from the blend of amphiphilic graft copolymer with short poly(ethylene glycol) chain and PLGA maintained suspension for 1 day when protein stock solution was introduced. This is somewhat improvement in colloidal stability against protein adsorption compared with that of nanoparticles without PEG moiety. Suspension stabilizer, PVA, had a significant effect on the colloidal stability against freezing and protein adsorption which led to coagulation of nanoparticles. It is important to consider effect of suspension stabilizer as well as materials used to prepare nanoparticle on the colloidal stability.

Effect of Powder Hardening Accelerator on the Physical Properties of Precast Concrete (분말형 경화촉진제를 혼입한 PC부재용 콘크리트의 기초특성에 관한 실험적 연구)

  • Jun, Woo-Chul;Seo, Hwi-Wan;Bae, Yeoun-Ki;Park, Hee-Gon;Min, Tae-Beom;Kwon, Yeong-Ho
    • Journal of the Korea Institute of Building Construction
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    • v.15 no.3
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    • pp.265-271
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    • 2015
  • This study is intended to produce a PC (Precast Concrete) member without a steam curing process in developing the high early strength concrete satisfying the condition of 10MPa in compressive strength at the age of 6 hours, and is intended to ensure economic feasibility by increasing the turnover rate of concrete form. Hence, high early strength cement with high $C_3S$ content and the hardening accelerator of powder type accelerating the hydration of $C_3S$ was used. And the properties of concrete were evaluated according to the hardening accelerator mixing ratio (0, 1.2, 1.6, 2.0). No big difference was found from the tests of both slump and air content. When 1.6 % or higher amounts of the hardening accelerator were mixed, the compressive strength of 10MPa was achieved at the age of 6 hours. From the test results of autogenous (drying) shrinkage and plastic shrinkage, it can be seen that there was a difference according to hydration reaction rate due to the addition of the hardening accelerator. However, it was shown that no problem arose with crack and durability. And it was shown that resistance to freezing-thawing, carbonation, and penetration were excellent.

Study on Adhesive Strength of Polymer Modified Cement Mortar for Maintenance in Concrete Structure (콘크리트 구조물 보수용 폴리머시멘트 모르타르의 부착강도 특성에 관한 연구)

  • Park, Sang-Soon;Kim, Jung-Heum
    • Journal of the Korea institute for structural maintenance and inspection
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    • v.14 no.5
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    • pp.128-135
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    • 2010
  • Polymer-modified cement mortar(PCM) has been widely used for strengthening of the concrete structures due to its excellent physical properties such as high strength and durability. Adhesive strength or behavior, on the other hands, between PCM and concrete is very important in strengthening the concrete member using PCM. Therefore the adhesive failure mechanism between PCM and concrete should be fully verified and understood. This study was performed to evaluate adhesive strength of PCM to the concrete by the direct pull-out test. In the direct pull-out tests, the adhesive strength under the various pre-treatment conditions such as immersion, thunder shower, freezing and thawing are evaluated. Also, the field direct pull-out test are performed to investigate the adhesive strength of mock-up test specimens. In the results of the test, the adhesive strength value by field test are lower than those of the standard curing condition. From these comparison and investigation, field test result was similar with the thunder shower test result. The results of the test was used to evaluate the korean industrial standard of polymer modified cement mortars for maintenance in concrete.

Effects of Freezing and Reheating on the Textural Characteristics of Mungbean Flour Gels and Mungbean Cake(Bindaedduk) (동결 및 재가열이 녹두가루겔 및 빈대떡의 조직 특성에 미치는 영향)

  • 고하영;우자원
    • Korean journal of food and cookery science
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    • v.15 no.4
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    • pp.353-357
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    • 1999
  • The textural characteristics of mungbean flour gels and mungbean cake(Bindaedduk) were investigated in steam and microwave reheating condition after 20$^{\circ}C$, 5$^{\circ}C$ and -18$^{\circ}C$ storage. The hardness of mungbean flour gels were 2.36 kg in microwave reheating and 3.59 kg in steam reheating after 6 days frozen storage, respectively and its gumminess were increased after reheating. The hardness of mungbean flour gels did not significantly change with the storage temperature. The textural characteristics of mungbean cake made with 2 parts of mungbean flour and 1/2∼1/8 parts of nonwaxy rice flour had the similar values in spite of the different compositions. Microwave reheated mungbean flour gels had the different hardness values of 4.13 kg in non package and 1.70 kg in polyethylene film wrap after 24 hours storage at 20$^{\circ}C$. In sensory evaluation mungbean flour gels showed the high scores in hardness and the unpleasant flavor after reheating but mungbean cake of different compositions showed the good sensory qualities.

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Estimating on the Erosion and Retreat Rates of Sea-cliff Slope Using the Datum-point in Pado-ri, the Western Coast of Korea (침식기준목을 이용한 파도리 해식애 사면의 침식·후퇴율 산정)

  • JANG, Dong-Ho;PARK, Ji-Hoon
    • Journal of The Geomorphological Association of Korea
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    • v.19 no.3
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    • pp.71-82
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    • 2012
  • This research was carried out to estimate annual erosion and retreat rates by using datum-point and to identify the characteristics and causes of seasonal variations of sea-cliff slope in Pado-ri, Taean-gun. In the result, the erosion and retreat rates of sea-cliff were increased from spring to summer. The rates were increased rapidly between August and October, caused by the effects of extreme weather events such as severe rainstorms and typhoons, etc. Since then, the erosion and retreat rates of sea-cliff were decreased gradually, but the rates were increased again in winter due to the storm surge and mechanical weathering resulting from the repeated freezing and thawing actions of bed rocks. The factors that affect erosion and retreat rates of sea-cliff include the number of days with antecedent participation and daily maximum wave height. In particular, it turned out that the erosion is accelerated by strong wave energy during storm surges and typhoons. The annual erosion and retreat rates of study area for the past two years(from May 2010 to May 2012) were approximately 44~60cm/yr in condition of differences in geomorphological and geological characteristics at each point. These erosion and retreat rates were found to be higher than results of previous researches. This is caused by coastal erosion forces strengthened by extreme weather events. The erosion and retreat process of sea-cliff in the study area is composed by denudation of onshore areas in addition to marine erosion(wave energy).

Cold Hardiness Change in Solenopsis japonica (Hymenoptera: Formicidae) by Rapid Cold Hardening (급속내한성 유기에 의한 일본열마디개미(Solenopsis japonica)의 내한성 변화)

  • Park, Youngjin;Vatanparast, Mohammad;Lee, Jieun
    • Korean journal of applied entomology
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    • v.60 no.2
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    • pp.193-199
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    • 2021
  • Solenopsis japonica, which is belonging to Formicidae in Hymenoptera, is a native ant species in Korea. However, it had not been studied for cold hardiness of S. japonica to understand on its overwintering mechanisms in field so far. Cold tolerance on developmental stages was measured at different cold temperature with various exposure times. Workers showed more survival at 5℃ and 10℃ compared with other stages and elevated cold tolerance when workers were exposed at 15℃ for more than 12h incubation as a rapid cold hardening (RCH) condition. RCH treatment not only increased survival of workers at cold temperatures, but also decreased supercooling point (SCP) and freezing point (FP). RCH group increased the survival rate by 44% at 10℃ compared with Non-RCH group. SCP and FP were depressed from -10.0 to -14.2℃ and from -11.3 to -15.3℃, respectively, after RCH treatment. Cold temperature increased expression level of cold- and stress-related genes such as glycerol kinase and heat shock protein. These results indicate unacclimated cold tolerance of S. japonica and its acclimation to low temperature by RCH.

On-site Conservation Treatment of the Beaker-shaped Pottery from Yori, Hyangnam, Hwaseong (화성 향남 요리 출토 심발형 토기 수습과 보존처리)

  • Kwon, Ohyoung;Ham, Chulhee;Lee, Sunmyoung
    • Journal of Conservation Science
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    • v.36 no.6
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    • pp.494-504
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    • 2020
  • Ten historic sites (denoted as A- J in this study) of a tomb were found during the construction of the east-west expressway in District 2 of Hyangnam, Hwaseong, which is implemented by the Gyeonggi-do headquarters of the Korea Land & Housing Corporation. Thetombswere first detected at siteH, and further investigations revealed various tombs from the Three Kingdoms period; artifacts such as gilt-bronze shoes and caps were excavated from wooden coffins in the tombs. The pottery examined in this study was the only pottery artifact excavated from the site. Its raw clay was soft and loose, reddish brown, and had quartz and feldspar particles of < 1 mm, which appeared to have been added as reinforcing agents. The firing temperature of the pottery was estimated to be under 800-870 ℃ as the mica remains and tridimite, which is the phase transition mineral of quartz, was not produced; a slight endothermic peak was also detected because of the hydration of sericite at 800 ℃. The condition of the artifact was severely weakened because of various factors, such as soil pressure from the stratum formed over the site and repeated freezing and thawing. The artifact could not be collected alone, and thus, surrounding soil that had attached to the artifact was also collected; the artifact was transported to the laboratory and conservation treatment was conducted in a safe and systematic manner.