• 제목/요약/키워드: freeze-concentration

검색결과 326건 처리시간 0.023초

수직온도구배냉각법으로 크롬과 인듐이 함께 도핑된 반절연 갈륨비소 단결정의 성장 및 특성평가 (Growth and characterization of semi-insulating GaAs co-doped with Cr and In by vertical gradient freeze technique)

  • Young Ju Park;Suk-Ki Min;Kee Dae Shim;Mann J. Park
    • 한국결정성장학회지
    • /
    • 제4권1호
    • /
    • pp.83-91
    • /
    • 1994
  • 직경 2인치 갈륨비소 단결정을 위한 수직온도구배냉각 결정성장치를 제작하여 크롬과 인듐이 함께 도핑된 반절연 갈륨비소 단결정을 성장하였다. 함께 도핑된 결정에 대해서,불순물의 편석계수는 크롬 또는 인듐만을 도핑한 결정에서 편석계수와 비교할 때 변하지 않고 같은 값을 갖는다. 크롬과 인듐의 농도는 결정의 종자부분 부터 꼬리부분까지 각각 $2{\Times}10^{16}~3{\Times}10^{17} cm^{-3}$$2{\Times}10^{19}~3{\Times}10^{20} cm^{-3}$의 범위내에서 분포되었다. 평균 전위밀도는 결정의 전 영역에 걸쳐 약 $8000cm^{-2}$ 미만으로 관측되었다. 또한 인듐농도가 증가함에 따라 전위 밀도가 $1000m^{-2}$ 미만으로 감소하는 격자강화의 효과도 관찰할 수 있었다. 갈륨비소의 결정성장 방향에 따라 캐리어의 농도는 $10^{16}$에서 $10^8cm^{-3}$로 감소한 반면 비저항 값은 $10^{-2}$에서 $10^8$ Ohm-cm로 급격하게 증가하였다. 반절연의 특성은 크롬농도 약 $6{\Times}10^{16}cm^{-3}$의 농도이상에서 얻을 수 있었다. 성장된 단결정 내의 깊은 준위는 두 개의 전자덫인 $E_C-0.81eV, E_C-0.35eV$와 두 개의 정공덫인$E_V+0.89eV, E_V+0.65eV$이다.

  • PDF

재수화용액의 염농도에 따른 동결건초 도토리 묵의 재수화 특성 (Effects of Salt Concentration on the Rehydration Characteristics of Freeze Dried Mook)

  • 윤광섭;황정섭;정헌식;양경미
    • 한국식품저장유통학회지
    • /
    • 제8권3호
    • /
    • pp.313-319
    • /
    • 2001
  • 도토리 묵을 인스턴트화 하기 위하여 생묵을 동결건조하여 재수화 시간, 재수화 용액의 염농도(0, 1, 2%) 및 온도(20, 70, 80, 9$0^{\circ}C$)에 따른 재수화 특성과 품질특성을 비교하였다. 동결건조묵의 최적 재수화 시간은 15분으로, Cooling 시간은 3분으로 각각 결정되었다. 재수화 효율은 재수화 용액의 염농도가 1%에서 가장 우수하였으며 재수화 온도가 높을수록 좋았다. 수분흡수속도상수도 재수화 온도가 증가할수록 놀았으며 1%의 염농도에서의 재수화가 온도변화에 따라 가장 놀은 활성화에너지를 가졌다. 동결건조묵의 재수화 후 표면색도와 재수화성 등을 고려해 볼 때 1% 염농도에서의 재수화가 묵의 품질에 우수한 것으로 평가되었다.

  • PDF

크랜베리 분말을 첨가한 파운드케이크의 품질특성 (Quality Characteristics of Pound Cake with Cranberry Powder)

  • 이성윤;정현철;유승석
    • 한국식생활문화학회지
    • /
    • 제30권6호
    • /
    • pp.750-756
    • /
    • 2015
  • This study was investigated cranberry powder substituted for flour in pound cake recipes with the amounts of 0, 4, 7, 10, and 13%. Cranberry powder have of 6.47% of moisture content, 3.38% of crude protein, 4.92% of crude fat and 0.43% of crude ash. No significant difference in weight was found from increased cranberry powder concentration. Volume and specific loaf volume have decreased as the cranberry powder content increased. No significant difference in moisture and brix was found from increased cranberry powder concentration. pH have decreased as the cranberry powder content increased. 'L' and 'b' decreased with an increase in the cranberry powder concentration, but 'a' increased. The texture measurement result showed that the hardness and chewiness of cake have decreased as the cranberry powder content increased. springiness, cohesiveness and adhesiveness showed no significant difference with the increasing amount of caked cranberry. Overall preference scores showed a high overall acceptability for the cake made with 7% cranberry powder.

Effect of Ecklonia cava Powder on Color and Texture of Sugar-Snap Cookies

  • Park, Myeong Ju;Lee, Jun Ho
    • 산업식품공학
    • /
    • 제15권1호
    • /
    • pp.85-89
    • /
    • 2011
  • Freeze-dried Ecklonia cava powder was incorporated into cookie dough at 5 levels (0%, 1.5%, 3%, 4.5%, and 6%, w/w) by replacing equivalent amount of wheat flour of the cookie dough. After aging and sheeting, cookies were baked at $185^{\circ}C$ for 14 min in a convection oven. The baked cookies were cooled to room temperature for 30 min and packed in airtight bags prior to all measurements. Lightness $(L^*)$ decreased significantly as the E. cava powder content increased (p<0.05) and a decreasing trend in both redness ($a^*$-value) and yellowness ($b^*$-value) was observed. On the other hand, firmness increased significantly with an increase in E. cava powder content (p<0.05). Increases in E. cava powder concentration up to 6% in the cookie formulation significantly increased the intensities of all sensory attributes such as color, flavor, taste, and firmness (p<0.05). Correlation analysis indicated that the E. cava concentration correlated significantly with most of the properties except for $a^*$-value (p<0.01, 0.05, or 0.001). Properties such as firmness and sensory color and firmness correlated positively while $L^*$- and $b^*$-value correlated negatively with E. cava concentration. Sensory color correlated negatively with $L^*$- and $b^*$-value. Sensory firmness correlated positively with mechanically measured firmness.

양막과 콜라겐을 이용한 생체 적합 드레싱 소재 개발 및 백서 창상치유 실험 (DEVELOPMENT OF BIOCOMPATIBLE DRESSING MATERIAL MADE OF COLLAGEN AND AMNIOTIC MEMBRANE AND WOUND HEALING EXPERIMENT IN RAT)

  • 안강민;이지호;이의룡;이종호;이종원;김성포;양은경;김기호
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
    • /
    • 제32권3호
    • /
    • pp.189-199
    • /
    • 2006
  • Purpose of study: Partial thickness skin graft is the golden standard regimen for full-thickness skin defect caused by burn or trauma. However, in case of extensive burns of more than 50% of total body surface area, the donor site is not sufficient to cover all defects. As a second choice, allograft, xenograft and synthetic materials have been used to treat skin defect. Among them the amniotic membrane(AM) was used as a biological dressing for centuries because of its potential for wound healing. In this study, quantification of EGF in AM and effect of AM-collagen complex on full thickness skin defects was examined. Materials & Methods: The concentration of EGF in fresh, deep frozen and freeze-dried AM was evaluated by ELISA. EGF-R immunostaining was performed in freeze-dried AM. SD rats weighing 250${\sim}$300g was used for wound healing experiment. Three full thickness skin defects(28mm diameter) were made on dorsal surface of SD rat. The control group was covered by Vaselin gauze and AM-collagen complex and $Terudermis^{(R)}$. was grafted in two other defects. Healing area, Cinamon's score were evaluated before biopsy. Grafted sites were retrieved at 3 days, 1 week, 2 weeks and 4 weeks after operation. H & E and Factor VIII immunohistochemical stain was performed to evaluate the microscopic adhesion and structural integrity and microvessel formation. Results: 1. EGF concentration of fresh, deep frozen and freeze-dried AM showed similar level and EGF-R was stained in epithelial layer of freeze-dried AM. 2. At 4 weeks after grafting, the healing area of AM-collagen and Terudermis group was 99.29${\pm}$0.71% and 99.19${\pm}$0.77 of original size. However, that of control group was 24.88${\pm}$2.90. 3. The Cinamon's score of AM-Collagen and $Terudermis^{(R)}$. group at 4 weeks was 15.6${\pm}$1.26 and 14.6${\pm}$3.13 and that of control group was 3.7${\pm}$0.95. Significant difference was observed among control and experimental groups(p<0.05). 4. Histologic examination revealed that AM protected leukocyte infiltration and epithelial migration was nearly completed at 4 weeks. $Terudermis^{(R)}$. group showed mild neutrophil infiltration until 2 weeks and completion of epithelization at 4 weeks. Control group showed massive leukocyte infiltration until 4 weeks. 5. Microvessels were increased sharply at 1 week and control group at 1 and 4 week showed significant differences with $Terudermis^{(R)}$. group of same interval(p<0.05) but no differences were found with AM group(p<0.05). Conclusion: EGF and EGF-R were well preserved in freeze-dried AM. AM attached to collagen acted as excellent biologic dressing which had similar effect with $Terudermis^{(R)}$. AM showed anti-inflammatory action and healing was completed at 4 weeks after full-thickness skin defect.

Takahashi 구름모형에서의 얼음입자 충돌효율 개선 (Implementation of Improved Ice Particle Collision Efficiency in Takahashi Cloud Model)

  • 이한아;염성수
    • 대기
    • /
    • 제22권1호
    • /
    • pp.73-85
    • /
    • 2012
  • The collision efficiency data for collision between graupel or hail particles and cloud drops that take into account the differences of particle density are applied to the Takahashi cloud model. The original setting assumes that graupel or hail collision efficiency is the same as that of the cloud drops of the same volume. The Takahashi cloud model is run with the new collision efficiency data and the results are compared with those with the original. As an initial condition, a thermodynamic profile that can initiate strong convection is provided. Three different CCN concentration values and therefore three initial cloud drop spectra are prescribed that represent maritime (CCN concentration = 300 $cm^{-3}$), continental (1000 $cm^{-3}$) and extreme continental (5000 $cm^{-3}$) air masses to examine the aerosol effects on cloud and precipitation development. Increase of CCN concentration causes cloud drop sizes to decrease and cloud drop concentrations to increase. However, the concentration of ice particles decreases with the increase of CCN concentration because small drops are difficult to freeze. These general trends are well captured by both model runs (one with the new collision efficiency data and the other with the original) but there are significant differences: with the new data, the development of cloud and raindrop formation are delayed by (1) decrease of ice collision efficiency, (2) decrease of latent heat from riming process and (3) decrease of ice crystals generated by ice multiplication. These results indicate that the model run with the original collision efficiency data overestimates precipitation rates.

농축 및 건조방법에 따른 생강 추출액 분말의 품질변화 (Quality of Ginger Powder as Affected by Concentration and Dehydration Methods of Ginger Extracts)

  • 정문철;정승원;이영춘
    • 한국식품과학회지
    • /
    • 제31권6호
    • /
    • pp.1589-1595
    • /
    • 1999
  • 생강 추출액의 저장안정성을 제공하기 위하여 적정농축 및 건조방법을 선정코자 감압증류 및 RO(역삼투압)에 의한 농축과 냉동 및 분무건조방법별로 분말화한 다음 관능특성을 비롯한 이화학적인 품질특성을 비교하여 보았다. 농축 및 건조방법별 분말제품을 제조한 결과 갈변도는 감압증류법이 RO보다. 분무건조가 냉동건조보다 갈변이 덜 진행된 상태인 반면에 당 함량이나 유리아미노산은 농축방법의 영향을 인식할 수 없었으나 건조방법에 있어서는 냉동건조시 대체로 이들 성분의 손실율을 적게할 수 있었다. 효소적 액화추출액 분말은 생강 추출액 분말보다 단백질과 조회분함량이 낮은 반면 조지방 함량은 약간 높게 나타났으며, 농축 및 건조 중 갈변도와 유리 아미노산의 변화율을 생강 추출액 분말보다 억제하면서 용해도를 증가시킬 수 있는 효과가 있었다. 또한 생강분말에 대한 기호도 검사결과, 농축방법에서는 생강 추출액 및 효소적 액화추출액에서 RO농축한 제품이 우수한 결과를 보였으며 건조방법에서는 냉동건조가 우수한 결과를 나타내었다. 특히 효소적 액화 추출액의 경우에는 RO농축한 후 냉동건조하면 종합 기호도에 있어서 생강추출액 분말에 대한 기호도 특성과 거의 차이가 없는 것으로 나타났다.

  • PDF

Protective Effects of the Fermented Laminaria japonica Extract on Oxidative Damage in LLC-PK1 Cells

  • Park, Min-Jung;Han, Ji-Sook
    • Preventive Nutrition and Food Science
    • /
    • 제18권4호
    • /
    • pp.227-233
    • /
    • 2013
  • This study investigated the protective effect of the butanol (BuOH) fraction from fermented Laminaria japonica extract (BFLJ) on AAPH-induced oxidative stress in porcine kidney epithelial cells (LLC-PK1 cells). L. japonica was fermented by Aspergillus oryzae at $35{\pm}1^{\circ}C$ for 72 h. Freeze-dried fermented L. japonica was extracted with distilled water, and the extracted solution was mixed with ethanol and then centrifuged. The supernatant was subjected to sequential fractionation with various solvents. The BuOH fraction was used in this study because it possessed the strongest antioxidant activity among the various solvent fractions. The BuOH fraction of fermented L. japonica had a protective effect against the AAPH-induced LLC-PK1 cells damage and increased cell viability while reducing lipid peroxidation formation and increased activities of antioxidant enzymes such as superoxide dismutase and glutathione peroxidase. The inhibitory effect of BFLJ on lipid peroxidation formation had a higher value of $0.11{\pm}0.01nmol$ MDA at $100{\mu}g/mL$ concentration in comparison with intact BuOH fraction showing $0.22{\pm}0.08nmol$ MDA at the same concentration. Furthermore, BFLJ treatment increased glutathione concentration. GSH concentration in the cell treated with BFLJ of $100{\mu}g/mL$ was $1.80pmol/L{\times}10^5cells$. These results indicate that BFLJ protects the LLC-PK1 cells against AAPH-induced cell damage by inhibiting lipid peroxidation formation and increasing antioxidant enzyme activities and glutathione concentration.

The Cryoprotective Effect on Frozen-thawed Boar Semen of Egg Yolk Low Density Lipoproteins

  • Hu, Jian-hong;Li, Qing-Wang;Li, Gang;Chen, Xiao-Yu;Hai-Yang, Hai-Yang;Zhang, Shu-Shan;Wang, Li-Qiang
    • Asian-Australasian Journal of Animal Sciences
    • /
    • 제19권4호
    • /
    • pp.486-494
    • /
    • 2006
  • In order to protect the spermatozoa against cold shock, hen egg yolk is widely used as a cryoprotective agent in semen freezing extenders for domestic animals. The protective action of yolk is largely presumed to be due to low density lipoproteins (LDL). The effects of LDL on sperm quality of bull and northern pike (Esox lucius) after freezing-thawing have been reported, but no study has been made to evaluate the effect of LDL on boar sperm motility and other characteristics. The experiment was carried out to investigate the effect of LDL on the freezing of boar sperm in 0.25 ml straws. The aim was to evaluate the quality of boar spermatozoa cryopreserved in the presence of LDL. Motility of semen cryopreserved in LDL was analyzed and compared to semen cryopreserved with Tris-citric acid-glucose (TCG) and Tris-citric acid-fructose (TCF), two basic freezing extenders containing egg yolk. Similarly, acrosome and plasma membrane integrity were also evaluated and compared to semen cryopreserved with TCG and TCF. Analysis of sperm quality after freeze-thaw showed that the motility, acrosome and plasma membrane integrity were improved with LDL in the extender, as compared to the TCG and TCF. The highest post-thaw integrity of acrosome and plasma membrane and motility were obtained with 9% LDL (w/v). Consequently, the optimum LDL concentration in the extender was 9%. It is also suggested that the concentration of LDL addition is important for the effect on boar sperm protection during freezing and thawing. The percentage of motile spermatozoa was significantly higher after freezing in 9% LDL than in TCG and TCF 54.4% versus 30.4% and 30.1% (p<0.05), respectively. The integrity of acrosome and plasma membrane were also significantly higher at 70.3% and 50.5% respectively with semen frozen in 9% LDL extender compared to TCG at 37.8% and 30.3% and TCF at 36.4% and 29.9%, respectively (p<0.05),. In conclusion, we propose that extender containing LDL extracted from hen egg yolk could be used as a cryoprotective media with a better efficiency than TCG and TCF. LDL improved boar semen quality, allowing better spermatozoa motility, acrosome and plasma membrane integrity after the freeze-thaw process. Furthermore, we found out that the extender with 9% LDL concentration significantly enhanced motility, acrosome and plasma membrane integrity of boar sperm after freezing and thawing.

생약재 추출물로부터 주름 개선 소재의 발굴 및 이를 이용한 화장품의 안정성 시험 (Screening of Anti-wrinkle Resource from Herbal Medicinal Extracts and Stability Test of Its Cosmetic Products)

  • 조은아;조은혜;최선주;박근형;김소영;정윤주;구창섭;하병집;장동일;채희정
    • 한국약용작물학회지
    • /
    • 제19권2호
    • /
    • pp.126-135
    • /
    • 2011
  • Various herbal medicinal extracts were examined for the development of cosmetic products with anti-oxidative and anti-wrinkle activity. First, total polyphenol contents and DPPH radical scavenging activities of herbal medicinal extracts were measured. Most herbal samples, except for DW extracts of Portulaca oleracea, Caesalpinia sappan, Taraxacum platycarpum, Carthamus tinctorius, and 70% EtOH extracts of Taraxacum platycarpum and Carthamus tinctorius, showed DPPH radical scavenging activity over 80% at a concentration of $400{\mu}g/m{\ell}$. SOD-like antioxidant activity of DW extracts of Syzygium aromaticum, DW extracts of Eriobtrya japonica and 70% EtOH extracts of Sophora japonica was measured as 40%, 35% and 80%, respectively at a dry matter concentration of $50{\mu}g/m{\ell}$. In elastase inhibition assay, DW extracts of Lycium chinense ($50{\mu}g/m{\ell}$) and 70% EtOH extracts of Areca catechu ($50{\mu}g/m{\ell}$) showed 50% and 40% of inhibition, respectively. At a concentration of $1.250{\mu}g/m{\ell}$, DW extracts of Lycium chinense and 70% EtOH extracts of Areca catechu showed 10% and 30% of collagenase inhibition, respectively. Skin and lotion samples were prepared using the two herbal extracts of high anti-wrinkle activity: Lycium chinense extract and Areca catechu extract. The storage stability of skin and lotion containing each of the selected herbal extracts was evaluated. pH and viscosity were used as stability indicators for the stability test under different storage temperatures and freeze-thaw cycle conditions. The skin and lotion containing each of DW extract of Lycium chinense and 70% EtOH extract of Areca catechu was showed high pH and viscosity stability. The skin and lotion containing DW extracts of Lycium chinense showed relatively higher stability than the skin and lotion containing 70% EtOH extract of Areca catechu, at cycle chamber and freeze-thaw conditions. In summary, these results indicated that cosmetics containing DW extract of Lycium chinense were relatively stable, and this herbal extract could be used as a stable functional cosmetic material.