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The Cryoprotective Effect on Frozen-thawed Boar Semen of Egg Yolk Low Density Lipoproteins

  • Hu, Jian-hong (College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry) ;
  • Li, Qing-Wang (College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry) ;
  • Li, Gang (College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry) ;
  • Chen, Xiao-Yu (College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry) ;
  • Hai-Yang, Hai-Yang (College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry) ;
  • Zhang, Shu-Shan (College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry) ;
  • Wang, Li-Qiang (College of Animal Science and Technology, Northwest Sci-Tech University of Agriculture and Forestry)
  • Received : 2005.09.15
  • Accepted : 2005.11.18
  • Published : 2006.04.01

Abstract

In order to protect the spermatozoa against cold shock, hen egg yolk is widely used as a cryoprotective agent in semen freezing extenders for domestic animals. The protective action of yolk is largely presumed to be due to low density lipoproteins (LDL). The effects of LDL on sperm quality of bull and northern pike (Esox lucius) after freezing-thawing have been reported, but no study has been made to evaluate the effect of LDL on boar sperm motility and other characteristics. The experiment was carried out to investigate the effect of LDL on the freezing of boar sperm in 0.25 ml straws. The aim was to evaluate the quality of boar spermatozoa cryopreserved in the presence of LDL. Motility of semen cryopreserved in LDL was analyzed and compared to semen cryopreserved with Tris-citric acid-glucose (TCG) and Tris-citric acid-fructose (TCF), two basic freezing extenders containing egg yolk. Similarly, acrosome and plasma membrane integrity were also evaluated and compared to semen cryopreserved with TCG and TCF. Analysis of sperm quality after freeze-thaw showed that the motility, acrosome and plasma membrane integrity were improved with LDL in the extender, as compared to the TCG and TCF. The highest post-thaw integrity of acrosome and plasma membrane and motility were obtained with 9% LDL (w/v). Consequently, the optimum LDL concentration in the extender was 9%. It is also suggested that the concentration of LDL addition is important for the effect on boar sperm protection during freezing and thawing. The percentage of motile spermatozoa was significantly higher after freezing in 9% LDL than in TCG and TCF 54.4% versus 30.4% and 30.1% (p<0.05), respectively. The integrity of acrosome and plasma membrane were also significantly higher at 70.3% and 50.5% respectively with semen frozen in 9% LDL extender compared to TCG at 37.8% and 30.3% and TCF at 36.4% and 29.9%, respectively (p<0.05),. In conclusion, we propose that extender containing LDL extracted from hen egg yolk could be used as a cryoprotective media with a better efficiency than TCG and TCF. LDL improved boar semen quality, allowing better spermatozoa motility, acrosome and plasma membrane integrity after the freeze-thaw process. Furthermore, we found out that the extender with 9% LDL concentration significantly enhanced motility, acrosome and plasma membrane integrity of boar sperm after freezing and thawing.

Keywords

References

  1. Andersson, M., T. Hellman, B. G. Holmstrom and L. Jokinen. 1992. Computerized and subjective assessments of post- thaw motility of semen from Finnish Ayrshire Al bulls in relation to nonreturn rates. Acta. Vet. Scand. 33(l):89-93
  2. Bogart, R. and D. Mayer. 1950. The effects of egg yolk on the various physical and chemical factors detrimental to spermatozoa viability. J. Anim. Sci. 9:143 https://doi.org/10.2527/jas1950.92143x
  3. Check, J. H., B. Shanis, A. Bollendorf, H. Adelson and E. Breen. 1989. Semen characteristics and infertility in ageing. Arch. Androl. 23:275-277 https://doi.org/10.3109/01485018908986851
  4. Cheon, Y. M., H. K. Kim, C. B. Yang, Y. J. Yi and C. S. Park.2002. Effect of season influencing semen characteristics, frozenthawed sperm viability and testosterone concentration in Duroc boars. Asian-Aust. J. Anim. Sci. 15(4):500-503 https://doi.org/10.5713/ajas.2002.500
  5. Clarke, R. N. and L. A. Johnson. 1987. Effect of liquid storage and cryopreservation of boar spermatozoa on acrosomal integrity and the penetration of zona-free hamster ova in vitro. Gamete. Res. 16:193-204 https://doi.org/10.1002/mrd.1120160302
  6. Comizzoli, P., R. Mauget and P. Mermillod. 2001. Assessment of in vitro fertility or deer spermatozoa by heterologous IVF with zona-free bovine oocytes. Theriogenol. 56:261-274 https://doi.org/10.1016/S0093-691X(01)00561-1
  7. Cook, W. H. and W. G. Martin. 1969. Egg lipoproteins. In: (Ed. E. Tria and A. M. Scanu). Structural and functional aspects of lipoproteins in living systems. New York: Academic Press. 579-615
  8. Correa, J. R. and P. M. Zavos. 1994. The Hypoosmotic swelling test: it's employment as an assay to evaluate the functional integrity of the frozen-thawed bovine sperm membrane. Theriogenol. 42:351-360 https://doi.org/10.1016/0093-691X(94)90280-1
  9. Cotirtens, J. L. and J. M. Rety. 2001. Numerical simulation for freezing and thawing mammalian spermatozoa Evaluation of cell injuries at different depth in bags or straws during all steps of the technique. Genet. Sel. Evol. 33(Suppl l):85-104
  10. Demianowicz, W. and J. Strezek. 1996. The effect of lipoprotein fraction of egg yolk on some of the biological properties of boar spermatozoa during storage of the semen in liquid state. Reprod. Dom. Anim. 31:279-280 https://doi.org/10.1111/j.1439-0531.1995.tb00051.x
  11. Evans, R. C., D. H. Bauer, S. L. Bandemer, S. B. Vaghelf and C. J. Flegal. 1973. Structure of egg yolk very low density lipoprotein. Polydispersity of the very low density lipoprotein and the role of lipovitellenin in the structure. Arch. Biochem. Biophys.154:493-500 https://doi.org/10.1016/0003-9861(73)90001-5
  12. Fazeli, A., W. J. Hage, F. P. Cheng, W. F. Voorhout, A. Marks, M. M. Bevers and B. Colenbrander. 1997. Acrosome-intact boar spermatozoa initiate binding to the homologous zona pellucida in vitro. Biol. Reprod. 56:430-438 https://doi.org/10.1095/biolreprod56.2.430
  13. Garcia-Lopez, Z., M. Ollero, T. Muino-Blanco and J. A. Cebrian- Perez. 1996. A dextran swim-up procedure for separation of highly motile and viable ram spermatozoa from semen plasma. Theriogenol. 46:141-151 https://doi.org/10.1016/0093-691X(96)00149-5
  14. Gillmore, J. A., J. Liu, A. T. Peter and J. K. Critser. 1998. Determination of plasma membrane characteristics of boar spermatozoa and their relevance to cryopreservation. Biol. Reprod. 58:28-36 https://doi.org/10.1095/biolreprod58.1.28
  15. Graham, J. K. and R. H. Foote. 1987. Effect of several lipids fatty acyl chain length and degree of unsaturation on the motility of bull spermatozoa after cold shock and freezing. Cryobiol. 24:42-52 https://doi.org/10.1016/0011-2240(87)90005-8
  16. Hammadeh, M. E., T. Georg, P. Rosenbaum and W. Shmidt. 2001. Association between freezing agent and acrosome damage of human spermatozoa from subnormal and normal semen. Andrologia. 33:331-336 https://doi.org/10.1046/j.1439-0272.2001.00462.x
  17. Hossain, A. M., B. Rizk, S. Barik, C. Huff, I. H. Thorneycroft. 1998. Time course of hypo-osmotic swellings of human spermatozoa: evidence of ordered transition between swelling subtypes. Hum. Reprod.13:1578-1583 https://doi.org/10.1093/humrep/13.6.1578
  18. Jeyendran, R. S., Van, Der, Ven, H. H., M. Perez-Pelaez, B. G. Grabo and L. J. D. Zaneveld. 1984. Development of an assay to assess the functional integrity of the human sperm membrane and its relationship to other semen characteristics. J. Reprod. Fertil. 70:219-225 https://doi.org/10.1530/jrf.0.0700219
  19. Kumar, A., J. Singh, A. S. Nanda and G. R. Pangaonkar. 2004. Quality and fertility of post thaw sephadex filtered bull semen. Asian-Aust. J. Anim. Sci. 17(6):755-759 https://doi.org/10.5713/ajas.2004.755
  20. Lamia Amirata, Daniel Tainturiera, Laetitia Jeanneaua, Chantal Thorina, Olivier Gerard, Jean Luc Courtensc and Marc Antond. 2004. Bull semen in vitro fertility after cryopreservation using egg yolk LDL: a comparison with optidyl, a commercial egg yolk extender. Theriogenol. 61:895-907 https://doi.org/10.1016/S0093-691X(03)00259-0
  21. Larson, J. L. and D. J. Miller. 1999. Simple histochemical stain for acrosomes on sperm from several species. Mol. Reprod. Dev. 52:445-449 https://doi.org/10.1002/(SICI)1098-2795(199904)52:4<445::AID-MRD14>3.0.CO;2-6
  22. Mladenovic, I., S. Micic and O. Genbacev. 1995. Hypo-osmotic swelling test for quality control of spermatozoa prepared for assisted reproduction. Arch. Androl. 34:163-169 https://doi.org/10.3109/01485019508987845
  23. Moussa, M., V. Martinet, A. Trimeche, D. Tainturier and M. Anton. 2002. Low density lipoproteins extracted from hen egg yolk by an easy method: cryoprotective effect on frozen-thawed bull semen. Theriogenol. 57:1695-1706 https://doi.org/10.1016/S0093-691X(02)00682-9
  24. Obando, H., T. Tamayo and C. Alvaro. 1984. Evaluation of some factors affecting swine spermatozoa during freezing. In: Proceedings of the Tenth International Congresson Animal Reproduction AI. Urbana. 2:193
  25. Pace, M. M. and E. F. Graham. 1974. Components in egg yolk which protect bovine spermatozoa during freezing. J. Anim. Sci. 39:1144-1149 https://doi.org/10.2527/jas1974.3961144x
  26. Polge, C., S. Salamon and I. Wilmut. 1970. Fertilizing capacity of frozen boar semen following surgical insemination. Vet. Rec. 87:424-428 https://doi.org/10.1136/vr.87.15.424
  27. Revell, S. G. and R. A. Mrode. 1994. An osmotic resistance test for bovine semen. Anim. Reprod. Sci. 36:77-86 https://doi.org/10.1016/0378-4320(94)90055-8
  28. Rodriguez-Martinez, H., B. Larsson, B. R. Zhang and L. Soderquist. 1997. In vitro assessment of viability and fertilizing capacity of bull spermatozoa. J. Reprod. Dev. 43:1- 11 https://doi.org/10.1262/jrd.97-435j1
  29. Rota, A., N. Penzo, L. Vincenti and R. Mantovani. 2000. Hypoosmotic swelling (HOS) as a screening assay for testing in vitro fertility of bovine spermatozoa. Theriogenol. 53:1415- 1420 https://doi.org/10.1016/S0093-691X(00)00284-3
  30. Roth, T. L., R. B. Weiss, J. L. Buff, D. E. Wildt and L. M. Bush. 1998. Heterologous in vitro fertilization and sperm capacitation in an endangered African antelope the scimitarhorned oryx (Oryx dammah). Biol. Reprod. 58:475-482 https://doi.org/10.1095/biolreprod58.2.475
  31. Stalhammar, E. M., L. Janson and J. Philipsson. 1994. The impact of sperm motility on non return rate in preselected dairy bulls. Reprod. Nutr. Dev. 34(l):37-45 https://doi.org/10.1051/rnd:19940104
  32. Trimeche, A., M. Anton, B. Renard, G. Gandemer and D. Tainturier. 1996. Quail egg yolk: A novel cryoprotectant for the freeze preservation of Poitou jackass sperm. Cryobiol. 34:385- 393 https://doi.org/10.1006/cryo.1997.2009
  33. Tsutsui, T. 1988. Functional properties of heat-treated egg yolk low density lipoprotein. J. Food Sci. 53:1103-1106 https://doi.org/10.1111/j.1365-2621.1988.tb13539.x
  34. Vazquez, J. M., E. Martinez, P. Garcia-Artigac and J. Roca. 1997. Hypoosmotlc swelling of boar spermatozoa compared to other methods for analyzing the sperm membrane. Theriogenol. 47:913-922 https://doi.org/10.1016/S0093-691X(97)00046-0
  35. Vazquez, J. M., E. Martinez, J. Roca, P. Coy and L. M. Pastor. 1993. Acrosome reaction of boar spermatozoa in homologous in vitro fertilization. Mol. Reprod. Dev. 36:84-88 https://doi.org/10.1002/mrd.1080360112
  36. Visser, D. and S. Salamon. 1974. Effect of composition of trisbased diluent on stirvival of boar spermatozoa following deepfreezing. Aust. J. Biol. Sci. 27:485-497 https://doi.org/10.1071/BI9740485
  37. Watson, P. F. and C. A. Martin. 1975. The influence of some fractions of egg yolk on the survival of ram spermatozoa at 5$^{\circ}C$. Aust. J. Biol. Sci. 28:145-152 https://doi.org/10.1071/BI9750145
  38. Yanagimachi, R. 1994. Mammalian fertilization. In: (Ed. E. Knobil and J. D. Neil). The physiology of reproduction. New York: Raven Press. 189-317
  39. Yi, Y. J., H. J. Ko, S. H. Lee, C. B. Yang, D. S. Son, H. K. Kim and C. S. Park. 2004a. In vitro fertilization and development of pig oocytes inseminated with boar sperm by different sperm washing media after thawing of the frozen straws. Asian-Aust. J. Anim. Sci. 17(2):164-167 https://doi.org/10.5713/ajas.2004.164
  40. Yi, Y. J., S. H. Lee and C. S. Park. 2004b. Comparison of semen characteristics, frozen-thawed sperm viability, testosterone concentration and embryo development between Yorkshire boar A and B. Asian-Aust. J. Anim. Sci. 17(5):612-616 https://doi.org/10.5713/ajas.2004.612
  41. Zhou, J. B., K. Z. Yue, M. J. Luo, Z. L. Chang, H. Liang, Z. Y. Wang and J. H. Tan. 2004. Effect of extenders and temperatures on sperm viability and fertilizing capacity of Harbin white boar semen during long-term liquid storage. Asian-Aust. J. Anim. Sci. 17(11):1501-1508 https://doi.org/10.5713/ajas.2004.1501

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