• Title/Summary/Keyword: free-lipid

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Effects of Jwagyuyeum and Woogyuyeum on Free Radical Generating Enzyme Activities and Lipid Peroxidation in Rat's Kidney (흰쥐의 신(腎)에서 과산화지질(過酸化脂質) 생성(生成) 및 Oxygen Free radical 생성계(生成系) 효소(酵素) 활성(活性)에 미치는 좌귀음(左歸飮)과 우귀음(右歸飮)의 영향(影響))

  • Yoon, Cheol-Ho;Jeong, Ji-Cheon;Shin, Uk-Seob
    • The Journal of Internal Korean Medicine
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    • v.17 no.1
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    • pp.265-277
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    • 1996
  • Jwagyuyeum and Woogyuyeum, being known to reinforce Kidney-yin and -yang, were tested for the effects of on free radical generating enzyme and lipid peroxidation in rat's kidney. In vitro, levels of lipid peroxide in tissues of brain were proportionally decreased to concentration of extracts prepared from Jwagyuyeum and Woogyuyeum. They were much more decreased, when lipid peroxidation was induced with ferrous iron (Fe+2), In vivo, after both herbs were administered to the rat, levels of lipid peroxide in kidney were decreased only in Jwagyuyeum. The enzyme activities and the ratio of type conversion of xanthine oxidase were decreased only in Jwagyuyeum. But, We can't see special changes in Woogyuyeum. The enzyme activities of aldehyde oxidase was lowered in both herbs, especially Jwagyuyeum was much more done. These results suggest that Jwagyuyeum and Woogyuyeum decrease the activities of free radical generating enzymes such as xanthine oxidase and aldehyde oxidase which form lipid peroxide.

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Studies on the Lipid Components of Fresh Ginseng, Red Ginseng and White Ginseng (수삼(水蔘), 홍삼(紅蔘) 및 백삼(白蔘)의 지방질성분(脂肪質成分)에 관한 연구(硏究))

  • Choi, Kang-Ju;Kim, Dong-Hoon
    • Korean Journal of Pharmacognosy
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    • v.16 no.3
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    • pp.141-150
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    • 1985
  • Lipid and fatty acid compositions of free lipids and bound lipids from fresh ginseng, red ginseng and white ginseng were studied by means of silicic acid column chromatography, thin-layer chromatography and gas-liquid chromatography. Free lipid and bound lipid contents in those three samples were 1.21 to 1.45% and 0.32 to 0.45%. Neutral lipid fractions in free lipids from the samples were 76.6 to 79.7%, while glycolipid and phospholipid fractions were 11.6 to 14.7% and 8.5 to 8.7%, respectively. The major lipids were triglycerides, sterol esters and hydrocarbons, diglycerides and free sterols in neutral lipids, sterol glucoside, monogalactosyl diglyceride, esterified steryl glycoside, digalactosyl diglyceride in glycolipids and phosphatidyl ethanolamine, phosphatidyl glycerol, phosphatidyl choline and phosphatidyl inositol in phospholipids. Fourteen kinds of even numbered and four kinds of odd numbered fatty acids were identified in the four lipid fractions (TL, NL, GL and PL) by GLC, and the main fatty acids were linoleic acid, palmitic acid, oleic acid and linolenic acid.

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Free Radical Scavenging Compounds of Polygoni Multiflori Ramulus

  • Li-Xun;Kim, Mi-Hee;Ko, Eun-Kyung;Jun, Jung-Yang;Oh, Myung-Hun;Shin, Hwa-Woo;Kim, Youn-Chul
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.381.2-381.2
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    • 2002
  • There is now increasing evidence that free radicals and active oxygen species are involved in a variety of pathological events. often associated with ageing. Free radical-mediated cell damage and free radical attack on polyunsaturated fatty acids result in the formation of lipid radicals. These lipid radicals react readily with molecular oxygen to produce peroxy radicals responsible for initiating lipid peroxidation. The peroxidation of cellular membrane lipid can lead to cell necrosis and considered to be implicated in a number of pathophysiological conditions as well as in the toxicity of many xenobiotics. (omitted)

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Studies on the Lipid Components of Various Ginsengs ll. Lipid and Fatty Acid Compositions of the Bound Lipids (각국 인삼의 지방질성분에 관한 연구 제2보, 결합 지방질중의 지방질 및 지방산 조성)

  • Choe, Gang-Ju;Kim, Man-Uk;Kim, Dong-Hun
    • Journal of Ginseng Research
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    • v.9 no.2
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    • pp.204-212
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    • 1985
  • Lipid and fatty acid compositions of the bound lipids in Panax quinquefolium (Korea, Japan and China), Panax quinquefolium (America, Canada) and Panax notoginseng (China) were studied by means of silicic acid column chromatography, thin-layer chromatography and gas-liquid chromatography. The could lipid contents in various ginsengs were 0.29 to 0.48%, in which neutral lipid fractions were 63.6 to 67.3%, glycolipid fractions 21.9 to 25.7% and phospholipid fractions 7.7 to 12.4%. The content compositions of neutral lipid fractions were lower and those of glycolipid and phospholipid fractions were higher in the bound lipids than in the free lipids from the various ginseng. The major components were fatty acids, diglycerides and free sterols in neutral lipid fractions, monogalactosyl diglyceride, sterol glucoside and esterified steryl g1ycoside in glycolipid fractions and phosphatidyl glycerol, phosphatidyl ethanolamine and phosphatidic acid in phosphoipid fractions. Seventeen fatty acids were analyzed in the four bound lipid fractions from the various ginsengs and main fatty acids were linoleic acid, palmitic acid and oleic acid. Total saturated fatty acid and palmitic acid contents were higher and total unsaturated fatty acid and linoleic acid contents lower in the total bound lipids than in the total free lipids from the various ginsengs.

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Free radical scavenging phenolic compounds of the leaves of Juglans sinensis

  • Kim, Mi-Hee;Ko, Eun-Kyung;Jun, Jung-Yang;Li-Xun;Oh, Myung-Hun;An, Nyeon-Hyoung;Kim, Youn-Chul
    • Proceedings of the PSK Conference
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    • 2002.10a
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    • pp.381.3-382
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    • 2002
  • Free radical-mediated cell damage and free radical attack on polyunsaturated fatty acids result in the formation of lipid radicals. These lipid radicals react readily with molecular oxygen to produce peroxy radicals responsible for initiating lipid peroxidation. The peroxidation of cellular membrane lipid can lead to cell necrosis and considered to be implicated in a number of pathophysiological conditions as well as in the toxicity of many xenobiotics. DPPH is known to abstract labile hydrogen and the ability to scavenge the DPPH radical is related to the inhibition of lipid peroxidation. (omitted)

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Thermal Acclimative Changes in the Different Lipid Fractions Composition of Fat Body of Eri-Silkworm, Philosamia Ricini (Ward.)

  • Singh, G.B.;Singh, M.K.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.4 no.1
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    • pp.13-17
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    • 2002
  • Present communication deals with quantitative determination of total lipid, triglycerides, total free fatty acids, phospholipids and total cholesterol in the fat body tissue of the silkworm adapted to low and high temperatures. At the end of spinning process is characterized by a marked cellular reorganization of the different lipid fraction of the fat body irrespective of thermal acclimation. Accordingly, the per cent composition of triglycerides of the total lipid is increased accompanied by a corresponding decrease in free fatty acids, phospholipids and cholesterol.

Changes in Eating Quality and Lipid Components of Tongil Rice Variety in Storage (통일미(統一米)의 저장에 따른 기호특성(嗜好特性) 및 지질성분(脂質成分)의 변화)

  • Hwangbo, Jeong-Sook;Lee, Su-Rae
    • Korean Journal of Food Science and Technology
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    • v.8 no.2
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    • pp.74-79
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    • 1976
  • Two rice varieties in Korea, an Indica type 'Tongil' and a Japonica type 'Jinheung', were stored at $5^{\circ}C$ and $30^{\circ}C$ for six months and the changes in organoleptic quality and lipid components were investigated to obtain the following results. In storage, both varieties brought about increases of hardness, cohesiveness and gumminess, and a decrease of adhesiveness among textural parameters of cooked rice. Storage at $5^{\circ}C$ showed no change in organoleptic quality but storage at $30^{\circ}C$ caused the occurrence of off-flavor in both varieties and a marked decrease of stickiness in Jinheung. Rice samples before storage contained 5-times more free lipids than bound lipids and free lipid content of Tongil was $70{\sim}80%$ of Jinheung. In storage, bound lipids tended to increase slowly while free lipids increased in both varieties at $5^{\circ}C$ and tended to increase in Tongil and to decrease in Jinheung at $30^{\circ}C$. In free lipid fractions of rice samples before storage were found 9 components including triglycerides, free fatty acids and sterol esters and in bound lipid fractions were found 8 components including free fatty acids and sterol esters, as the major components. In storage, changes in lipid components were more remarkable in free lipids than in bound lipids and in Jinheung than in Tongil. It was, therefore, concluded that Tongil variety had a better storage stability than Jinheung variety and the deterioration of rice quality occurred mainly in free lipid fractions during storage.

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Enhanced Occlusiveness of Nanostructured Lipid Carrier (NLC)-based Carbogel as a Skin Moisturizing Vehicle

  • Choi, Woo-Sik;Cho, Hye-In;Lee, Hyun-Young;Lee, Seo-Hyun;Choi, Young-Wook
    • Journal of Pharmaceutical Investigation
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    • v.40 no.6
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    • pp.373-378
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    • 2010
  • In order to develop a topical preparation which has a high occlusive property with skin moisturization, nano-structured lipid carrier (NLC) systems along with solid lipid nanoparticle (SLN) were designed. Various NLC dispersions were successfully formulated with Compritol 888 ATO as a solid lipid, Labrafil M 1944 CS as an oil, and Tween 80 as a surfactant. The increase of oil content (5 to 50%) led to the decrease in the occlusion factor in the order of SLN > NLC-5 > NLC-15 = NLC-30 > NLC-50. Particle size of lipid particulates was in the range of 100 to 160 nm. NLC-based carbogels were prepared by the employment of humectants such as urea, glycerin, and Tinocare GL to carbomer gel. NLC-30 gel formulations containing 4 or 8 % of lipid particles showed improved occlusive effect in vitro, compared to NLC-free gel base. Even though NLC-free gel base revealed comparable occlusion effect by itself, the occlusion factor of 4 % NLC-30 gel was about 2-fold higher than that of NLC-free gel base.

Studies on the Lipid of Aquatic Products(Part 5) -Comparison of flesh Lipid Composition of Some Fresh Water Fishes Mandarin Fish Korean Perch Cornet Fist and Cat Fish- (수산물의 지질에 관한 연구(제5보) -쏘가리, 꺽지, 누치 및 메기의 근육지질 조성의 비교-)

  • 하봉석;강동수
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.19 no.4
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    • pp.291-300
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    • 1990
  • The lipid compositions including fatty acid sterol lipid class and the lipid component of the total lipid extracted from the flesh of some fresh water fishes I. e. mandarin cornet cat fish and Korean perch were compared. The levels of total lipid of flesh tissues from the fresh water fishes were high(7.4%) in cat fish but low(1.4-2.2%) in mandarin fish Korean perch and cornet fish. On the contrary the content of unsaponifiable matters found in total lipid was low(2.6%) in cat fish but high(6.0%-6.5%) in mandarin fish Korean perch and cornet fish. Total lipids were mainly composed of triglyceride(74.6-86.5%) as major component in each sample and the other lipid components of total lipid e. g. polar lipid free fatty acids and free sterol were the minor components, The major fatty acids in total lipid of each sample were{{{{ {C }_{16 { }:_{ }0 } }}}}(19.6-29.2%) {{{{ {C }_{16 { }:_{ }1 } }}}}(17.3-30.7%) and {{{{ {C }_{18 { }:_{ }1 } }}}}(16.8-29.2%) and additionally it chiefly consisted of {{{{ {C }_{14 { }:_{ }0 } }}}} and {{{{ {C }_{18 { }:_{ }2 } }}}} Particularly the contents of polyenoic acids in total lipid of cat fish were higher than those of the other fish samples. The level of cholesterol in total lipid was low (8.3mg/g) in cat fish but were high(36.9-59.9mg/g) in mandarin fish Korean perch and cornet fish. The contents of fractionated neutral lipid(NL) were higher than those of polar lipid(PL) in each sample. Particularly phospholipid content in PL was low(6.0%) in cat fish but were high(23.1-36.3%) in mandarin fish Korean perch and cornet fish. Neutral lipids were mainly consisted of triglyceride(84.5-93.4%) as amjor component in each sample and the other lipid components of neutral lipid e, g, free fatty acids and free wterol were exhibited as a minor components, The fatty acid compositon of neutral lipids was very resembled to total lipids. The phospholipid in mandarin cornet and cat-fish were mainly composed of phosphatidyl serine(23.1-49.8%) and phosphatidyl choline(20.8-45.3%) The relatively higher amounts of phosphatidyl serine were observed in mandarine cornet and cat-fist than in Korean perch, But phosphatidyl ethanolamine(42.3%) and phosphatidyl choline (49.9%) were the main phospholipid in Korean perch. The extraordinary high content of phos-phatidyl ethanolamine compared to other fishes was characteristics in phospholipid composition of Korean perch. The major fatty acids in phospholipid of each sample were {{{{ {C }_{16 { }:_{ }0 } }}}} (38.3-46.5%) {{{{ {C}_{18 { }:_{ }0 } }}}}(14.2-21.7) and C16:1(11.6-13.8%) and additionally it chiefly consisted of C18:2, C18:0 and C17:0 The major fatty acids in glycolipid of each sample were C16:0(28.8-40.1%) C18:1(5.4-29.9%) C18:0(5.1-28.9%) and C16:1(8.2-20.1%) and additio-nally it chiefly consisted of C14:0 and C20:1.

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Studies on the Production of Lipid by Microorganism (균체 지질 생산에 관한 연구)

  • Kim, Il -Young;Chung, Dong-Hyo
    • Korean Journal of Food Science and Technology
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    • v.17 no.2
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    • pp.81-88
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    • 1985
  • A potential lipid producing strain of Penicillium sp. was isolated from natural source. Penicillium sp. was cultivated in an appropriate medium containing 6% of glucose as a corbon source, ammonium nitrate as a nitrogen source, C:N ratio 200, pH 4.0 for a period of 17 days at $35^{\circ}C$. Under the condition, the lipid content was 64.2% of dry cell weight. The total lipid produced was 13.7g/100g of glucose consumed. The proportion of nonpolar and polar lipid fractions was 92.2% and 7.8%, respectively. The nonpolar lipid compositions of lipid produced under optimum condition were 5.3% of free fatty acids, 6.8% of free sterols, 9.3% of partial glycerides and 72.0% of triglycerides. The major fatty acids of total lipid were 20.1% of palmitic acid, 21.6% of linoleic acid and 53.3% of oleic acid.

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