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Effects of Ethylacetate Fraction of Plantago asiatica L. on Hypercholesterolemia Induced by High Cholesterol Diet in Rats (질경이(Plantago asiatica L.) 에틸아세테이트분획이 흰쥐의 고콜레스테롤혈증에 미치는 효과)

  • Lee Jae-Joon;Lee Jeong-Hwa;Jeong Chang-Ju;Choi Hyun-Sook;Lee Myung-Yul
    • Food Science and Preservation
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    • v.12 no.6
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    • pp.624-630
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    • 2005
  • Plantain was extracted with ethanol and fractionated systemically with n-hexane, chloroform, ethylacetate, n-butanol and water to study inhibitory effect on cholesterol synthesis in vitro. To screen the effect, inhibitory activities on 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase obtained from Saccharomyces cerevisiae were examined using the five fractions of Plantain. The HMG-CoA reductase activity was inhibited most by ehylacetate fraction among the fractions, although the all five fractions had the effect To see the hypocholesterolemic effect of the ethylacetate fraction of Plantanin (PAE) in vivo, male Sprague-Dawley rats were received 5 types of diets for 6 weeks: normal diet group (NOR), high cholesterol diet group($1\%$ cholesterol and $0.25\%$ sodium cholate, CON), normal diet and PAE 70 mg/kg administered group(S1), high cholesterol diet and PAE 140 mg/kg administrated group(S2), and high cholesterol diet and PAE 140 mg/kg administered group(S3). Body weight gains of the CON were significantly increased compared to those of S1, S2 and S3. Activities of serum AST and ALT were tended to be increased in CON compared with NOR and reduced by the PAE administration. Concentrations of serum total cholesterol, free cholesterol, LDL-cholesterol, triglyceride and the atherogenic index were tended to be decreased in the PAE administered groups compared with the CON. HDL-cholesterol and phospholipid concentrations were significantly decreased in the CON and markedly increased by the PAE administered groups. Taten together, it is suggested that the ethylacetate fraction of Plantanin exerts antiatherosclerotic effect by reducing serum cholesterol concentrations in rats fed high cholesterol diets.

Major Components of Teas Manufactured with Leaf and Flower of Korean Native Camellia japonica L. (국내 자생 동백나무의 잎과 꽃으로 만든 엽차와 화차의 주요성분)

  • Cha, Young-Ju;Lee, Jang-Won;Kim, Ju-Hee;Park, Min-Hee;Lee, Sook-Young
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.3
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    • pp.183-190
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    • 2004
  • The major compositions of leaf tea and flower tea were investigated to develope as a new functional tea using Korean native Camellia japonica L. Most of leaf teas, except flower tea, were considered as good materials with basic conditions for tea manufacture because water content was below 6%. Crude protein was the greatest component in roasted young leaf tea (RYLT), crude fats in roasted mature leaf tea (RMLT) and ashes in fermented young leaf tea (FYLT). Caffein were present as the highest amount (5.18%) in steamed mature leaf tea (SMLT), showing less amount than green tea. Catechin were contained as the highest amount in all kinds of teas, especially FYLT was the highest (9.57%). Tannin, which highly related with tea quality including astringent taste, color and perfume, were present as the highest amount in FYLT. Vitamin C was highly detected in the tea from flowers (22.7 mg/l00 g) rather than in the tea from leaves. The content of theanine were found in flower tea by 1,074 mg/l00 g, and had about twofold of FYLT and RYLT. Among free amino acids, glutamic acid and aspartic acid were higher detected in SMLT and RMLT while asparagine was present as higher amounts in RYLT and FYLT, expecting these components can improve tea taste. Nucleic acids and their derivatives including GMP, hypoxanthine and AMP were detected as the higher amounts by 7.86, 8.57, and $12.67\;{\mu}mol/g$, respectively, however IMP content was even reduced by all manufacturing processes. In all kinds of tea, sugars such as glucose, fructose, sucrose and maltose were detected, specially glucose and fructose were found as highest amount in RFT by 65.5 and 59.6 nmol/0.1 mg, respectively.

Biological Activity of Ixeris dentata Nakai juice Extracts (씀바귀(Ixeris dentata Nakai) 생즙 추출물의 생리활성)

  • 김명조;김주성;강원희;조미애;함승시;정동명
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.31 no.5
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    • pp.924-930
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    • 2002
  • Ixeris dentata extracts exllibited antimicrobial activity against some bacteria and fungi. Also EtOH extracts showed strong antioxidant activity and RC$_{50}$ value was 28 $\mu\textrm{g}$/mL. The inhibitory effect of Ixeris dentata on the mutagenicity in Salmonella and cytotoxicity on cancer cell were studied. Ixeris dentata extracts showed anti-mutagenic effects of 78.83 and 75.96% on B(a)P in S. typhimurium TA98 and Th100, respectively. These extracts showed 78.72% antimutagenicity on TA100 against MNNG. The Ixeris dentata extract with strong antimutagenic activities was further fractionated by hexane, ethyl acetate, butanol and water. Butanol fraction was found to be highest in antimutagenic activity against MNNG than the other fractions. Butanol fraction of Ixreis dentate revealed the highest cytotoxicity against AS49 human lung carcinoma cells in which cell growth was inhibited by 93.75% at 375 $\mu\textrm{g}$/mL. Hexane fraction of ixeris dentate exhibited 68.56% inhibition against MCF-7 human breast adenocarcinoma cells at 500 $\mu\textrm{g}$/mL. Hexane fraction of Ixeris dentata exhibited 84.91% inhibition against Hep 3B human hepatocellular carcinoma cells at 500 $\mu\textrm{g}$/mL. From these results, it is considered that Ixeris dentata has strong antimutagenic and anticancer effects in vitro. However, these extracts and fractions did not show any cytotoxic effect against 293 cells.

Comparison of the Dietary Factors between Normal and Osteopenia Groups by Bone Mineral Density in Korean Female College Students (골밀도 분류에 의한 정상군과 골감소증군 여대생의 식사관련 요인 비교)

  • Choi, Ji-Hee;Kim, Soon-Kyung
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.37 no.7
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    • pp.869-878
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    • 2008
  • The purpose of this study was to obtain baseline data on nutritional management of women's bone health. We examined the bone mineral density (BMD) by ultrasound bone densitometer, anthropometric measurement and dietary intake to assess the nutrient intakes. The subjects were 102 Korean female college students (normal=59, bone disease group [osteopenia+osteoporosis]=43) and the mean age was 21.4 yr. Mean T-score (BMD) were -0.42 and -1.52 in normal and bone disease groups, respectively. Anthropometric measurement showed that 59% of the subjects were normal weight and bone disease group had lower value than normal group in majority of anthropometric index. The average energy intake was 1539.7 kcal, which was 73% of Korean EER. The subjects had lower vitamin C, folic acid, Ca and K intake than Korean RI independent of BMD. Bone disease group (1:2.05) showed significantly lower Ca:P ratio than normal group (1:1.86). Normal group had more intake frequency of milk and milk product than bone disease group. In relation to anthropometric index with T-score, significant correlations have been found in weight, PM, BFM, MM, TBW, SLM, FFM WHR, BMI and SMM. In relation to dietary factors with T-score, significant correlations have been found relating to intake frequency of milk and milk product. Our results indicate that for nutrients, ratio and balance may be more influential than intake for bone health in young women.

Vegetation structure and environmental factors in Paksil-nup Wetland, Hapcheon (박실늪의 식생 구조와 환경 요인)

  • Kim, Cheol-Soo;Lee, Pal-Hong;Son, Sung-Gon;Oh, Kyung-Hwan
    • Journal of Wetlands Research
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    • v.2 no.1
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    • pp.31-40
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    • 2000
  • The flora, vegetation structure and physico-chemical characteristics of surface sediment were investigated in Paksil-nup wetland of Hapcheon-gun, Gyeongsangnam-do, Korea in 1990~1998. Ranges of the surface sediment characteristics such as pH, conductivity, organic matter, total nitrogen, available phosphorus, exchangeable K, exchangeable Ca, exchangeable Mg, and exchangeable Na were 4.36~4.34, $19.0{\sim}1260.0\;{\mu}mho\;cm^{-1}$, 0.01~6.35%, 0.001~0.14%, 0.01~0.31 mg/l00g, 1.01~13.98 ppm, 16.75~143.80 ppm, 0.93~14.85 ppm and 0.21~3.86 ppm, respectively. Percentages of the particle size such as sand, silt, and clay were 13.0~93.3%, 5.4~71.7%, and 0.5~37.5%, respectively. The flora of the study area was composed of 72 families, 182 genera, 223 species, 36 varieties and 2 form or total 261 kinds, and those were 45 kinds of vascular hydrophytes and 216 kinds of vascular hygrophytes. The life form of vascular hydrophytes was classified as 27 kinds (60%) of emergent plants, 8 kinds (18%) of submerged plants, 6 kinds (13%) of free-floating plants and 4 kinds (9%) of floating-leaved plants, respectively. The importance value of Salix nipponica was highest as 123.78 in the shrub and tree layers, and that of Trapa japonica was highest as 16.69 in the herb layer. The vegetation type was divided into two groups according to the association analysis. The vegetation of the littoral zone was classified into 7 associations according to the cluster analysis based on the coverage data. These results showed significant differences with those of stand ordination by correspondence analysis based on the species composition and by PCA based on the sediment properties. Factors affecting the distribution of the vascular hydrophytes and hygrophytes were the gradient of particle size, altitude, and water depth.

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A study on the pursuit of reality and elegance expressed in Jungchull's Sijo (정철 시조에 나타난 현실 지향과 풍류의 성격)

  • Jeon Jae-Gang
    • Sijohaknonchong
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    • v.21
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    • pp.207-239
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    • 2004
  • Jungchull(정철) was a very famous poet of Kasa(가사), Sijo(시조) and Hansi(한시) during the middle period of the Chosun kingdom. He has been studied by modern scholars mainly as a poet of Kasa(가사). These days, however, his Sijos are being studied as an important literary achievement. The purpose of this study is to analyze the pursuit of reality and elegance expressed in Jungchull's Sijo in order to understand his Sijos more deeply. In the process of this discussion, I will take a look at some of his Kasa(가사), Hansi(한시) and biography also, and I will compare his Sijo(시조) with that of his teacher's, friend's and senior's. Jungchull considered it important to become an official in the royal court because he was a Confucian scholar who believed in jugi-theory (주기론), in which all realistic phenomena is evaluated(Confucianism is very realistic in essence), and he had a happy life in the royal household until he was ten years old. I found that characteristics of his "realistic pursuit" are represented in his Sijo by two situations: the one is when he is apart from the King, and the other when he is together with the King. In all situations, though, he pursued an official position in the royal court. When he was apart from the king, his absolute resolution to follow the King was expressed in his not being able to sleep. In his sijo, he asks the wild duck to inform him of the King's welfare. He tries to flap his wings, to be the water or the moon in order to reach the royal household where the King was living. When he recognizes it's impossible, he expresses his perfect loyalty to the King in his Sijo by using literary paradox. According to this, he describes himself as not an able scholar, but also not a flattering scholar. In his sijo, he reminisces about working for the King in the royal household, when he had a good relationship with the King. But when he was an official in the province in a good relationship with the King, he expressed his intention of coming back to the royal household in order to be with the King again. From this we can ascertain that his pursuit of reality means to occupy an official position in the royal household, to serve the King and to have political power among of the officials. His elegance, expressed in his sijo, is deeply related to his absolute political pursuit of the King. He describes two kinds of elegance: nature related elegance and drinking alcohol related elegance. Two kinds of nature related elegance were delineated in his sijo, depending upon his political situation. When he had a good relationship with the King, occupying an official position, he delineated his gladness and free-heart in harmonizing with nature. But when he lost his official position. he expressed his purity using nature, and exclusion from nature. These kinds of nature related elegance were different from that of the usual Confucian scholar, for example, contemporaries Lee Hwang Next, two kinds of drinking alcohol related elegance were delineated in his sijo depending upon his political situation. More often he depicted excitement and composure of mind in his sijo by drinking alcohol in good situations than in efforts to overcome his agony in bad situations. The tradition of overcoming one's agony by drinking alcohol in bad situation was discovered by this writer in Jungchull's teacher's, and also his junior's sijo. Lastly in my next research paper future, I will look at the reason why certain various themes are represented in Jungchull's sijo, and what their the relationships are, to understand his literary works as a whole.

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Effect of Antioxidant, Antimutagenicity and Anticancer of Root Extract from Ixeris dentata Nakai (씀바귀 뿌리 추출물의 항산화성, 항돌연변이원성 및 항암활성 효과)

  • Kim, Myong-Jo;Kim, Ju-Sung;Jeong, Dong-Myong;Ham, Seung-Shi;Yu, Chang-Yeon
    • Korean Journal of Medicinal Crop Science
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    • v.10 no.3
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    • pp.222-229
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    • 2002
  • Ixeris dentata root were extracted with methanol and then fractionated with n-hexane, EtOAc and BuOH to get active fractions. and their antioxidant and antimicrobial activities in each fraction were determined. Ethyl acetate fraction of Ixeris dentata root showed strong antioxidant activities, but aqueous fraction did not show any activities. But in the antimicrobial test, aqueous fraction showed strong antimicrobial activities except to Escherichia coli. especially, aqueous fraction showed the strongest activities against Hypocrea nigricans. and butanol fraction showed the strongest activities against Cladosporium herbarum. This study was performed to determine the antimutagenic and cytotoxic effect of Ixeris dentata root methanol extract on Salmonella typhimurium TA98, TA100 and cancer cell lines using ames test and cytotoxicity assay, respectively. Cancer cell lines include human lung carcinoma(A549), human breast adenocarcinoma(MCF-7) and human hepatocellular carcinoma (Hep 3B). Futher fractionations with hexane, ethyl acetate, butanol and water from methanol extract of Ixeris dentata root were performed to obtain effective fraction, methanol extracts showed 79.94% inhibitory effect on the mutagenesis induced by N' -methyl- N' -nitro-N-nitrosoguanidine(MNNG) against TA100, while 89.99% inhibition was observed on the mutagenesis induced by 4-nitroquinoline-1-oxide(4NQO) against TA98. In the meanwhile, butanol fraction showed 89.92% and 71.01% inhibitory effect on the mutagenesis induced by benzo(a)pyrene(B(a)P) against TA98 and TA100, respectively. Ethyl acetate fraction showed the strongest effect against A549, MCF-7 and Hep3B at the same concentration compared to those of other fration.

Effect of Pyroligneous Liquor on Oxygen Radicals and Their Scavenger Enzymes in Liver of CD Rats (흰쥐 간조직의 활성산소 및 제거효소에 미치는 목초액의 영향)

  • Choi, Jin-Ho;Cho, Weon-Ki
    • Journal of Nutrition and Health
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    • v.40 no.2
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    • pp.111-117
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    • 2007
  • This study was designed to investigate the effects of pyroligneous liquor on oxygen radicals and their scavenger enzymes in the liver of Cri/Bgi CD rats (7 rats per group). Male rats were fed a basic diet prepared in our Lab., PL-0 (Control), PL-1, PL-25, PL-50 and PL-75 groups were Prepared to be 0%, 1%, 25%, 50% and 75%with distilled water using pyroligneous liquor (35% of Choa Co. Ltd.), and were administrated orally for 8 weeks. Superoxide radical contents in liver mitochondria and microsomes were significantly decreased to 12-14%, 11-15%, respectively, in these PL-25 and PL-50groups compared with the control group. Hydroxyl radical content in mitochondria and microsomes were markedly decreased to 12-20% and 17%, respectively, in these PL-25 and PL-50% groups compared with the control group. Hydrogen peroxide content in mitochondria and microsomes were significantly decreased about 15-12% and 22-20% in liver of PL-25 and PL-50 groups compared with the control group. Mn-SOD and Cu/Zn-SOD activities in liver of PL-25 and PL-50 groups were remarkably increased to 15-25%, 11-16%, respectively, compared with the control group. GPx activities in mitochondria and microsomes were significantly increased in the liver of PL-25 and PL-50 groups compared with the control group. CAT activities in mitochondria and cytosol were significantly increased to 12-14%, 15-27%, respectively, in the liver of PL-25 and PL-50 groups compared with the control group. These results suggest that long term administration orally of 25 and 50% pyroligneous liquor may effectively inhibit the formation of oxygen free radicals, and also scavenger enzyme activities significantly increase through the administration orally.

Development of the Holocene Sediments in Gamak Bay of the South Sea, Korea (남해 가막만의 현생퇴적층 발달특성)

  • Kim, So Ra;Lee, Gwang Soo;Choi, Dong Lim;Kim, Dae Choul;Lee, Tae Hee;Seo, Young Kyo
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.19 no.2
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    • pp.131-146
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    • 2014
  • High-resolution seismic profiles coupled with sediment sampling were analyzed to investigate the acoustic characters and distribution patterns of the late Holocene sediments in Gamak Bay of the South Sea, Korea. The mean grain size of surficial sediment lies around $6.3{\sim}9.7{\Phi}$. Sediments in the bay consist of silt and clay with progressive decrease toward the inner bay. The seismic sedimentary sequence overlying the acoustic basement can be divided into two sedimentary units (GB I and II) by a prominent mid-reflector (Maximum Flooding Surface; MFS). The acoustic basement occurs at the depth between 20 m and 40 m below the sea-level and deepens gradually southward. The GB I, mostly occupying the channel-fill, is characterized by reflection-free seismic facies. It can be formed as late Transgressive System Tract (TST), interpreted tidal environment deposits. MFS appears at the depth of about 15~28 m below the sea-level and is well defined by even and continuous reflectors on the seismic profile. The GB II overlying MFS is composed of acoustically transparent to semitransparent and parallel internal reflectors. GB II is interpreted as the Highstand System Tract (HST) probably deposited during the last 6,000 yrs when the sea level was close to the present level. Especially, it is though that the GB II was subdivided into two layers (GB II-a and II-b) by a HST-reflector and this was classified by wind, sea water flux, and tidal current.

The Behavior and an Attitude for Weight Control of High-School Students (고등학생의 체중조절에 대한 관련 행동 및 태도)

  • Choi, Jong-Cheol;Park, Young-Soo
    • The Journal of Korean Society for School & Community Health Education
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    • v.3
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    • pp.59-78
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    • 2002
  • The purpose of this study was based on students in high school to find out how interest eating and controlling weight on normal time by sex to let them get a better sense's of view on weight controlling so they can get healthy, and healthy school life and concentrate on studying, As a result of analyzing the data collected for the subject of high school students, the conclusions were as follows; First, out of 325 students, 44.6% were male students, and 179 were female students, Compared to the BMI, normal mass were 50.8%, which were 165 students, less weighted were 39.7%, which were 129 students, and over weighted were 9.5%, which were 31 student. Also average height for male students were 173.8 cm, for female students were 161.5 cm, average weight were 67.2 kg for male students, and for female students were 53.2kg. Using BMI analysing the results and the male students had an average of 22.2%, and the female students had an average of 20.3%, so male students were a little higher than the female students. Second, the interest rate for weight control were 82.2%, that's 267 students for, 'interested' and 7.8%, which were 58 student for 'not interested', so most students were interested, in controlling weight. Interest rate were 83.6% for male students, 81.0% were female students. BMI told that group of less weighted were 82.9%, group of normal were 79.4% and group of over weighted were 93.5%. The rate were all high not relating to BMI. Third, compared to the past, more people said 'normal'(41.3%), people who said 'a little fat'(36.3%) decreased, but they still think they are fat even though they are not, also when they are less weighted they still think they are fat. Fourth, for 'weight control, and food' both male and female said they were related, and for 'weight control and exercising' they also said they were related, but more male said that they were related, However for relations between' controlling weight and school's physical education class' the answers were usually disagree. Fifth, for the 'satisfaction of their present weight', both BMI and the students answered and this results were mostly same as the past result, so most students prefer to lose weight. Also, both male and female think that the reason they have this weight now is, because of 'the amount of exercises' and 'the amount of food they eat', so they find that it's related to each other. Sixth, for the experience on weight control, both male and female had experiences, and they answered 'exercising and food treatment' is the good way to control weight. Also for 'the reason they started to control there weight', both male and female answered, 'they thought there weight were not normal'. Seventh, 'Do you pick on food to control weight?' and 'Do you feel nervous before you eat?' and for last 'control of drinking water' the answer was all different, and both male and female answered negatively. Eighth, time wasted on exercising per day, for less than 30 minute were 81.5%, the form of exercises that students did were 'not much or walking on the way to school and way to home'. Usually for their free time, male students spend on exercising however female student did not. Also both female and male students showed that they like to exercise, but majority of female student disliked to exercise. To everyone's point of view 'like'(32.9%), 'like a lot'(20.9%), so everyone agrees. The knowledge information on controlling weight, they answered, usually found from commercials, newspaper or magazines and from parents or friend. From the past many high school students wanted to control their weight, so there should had been a good education on this, however there wasn't any of those education and still it doesn't exist. Also most school's education are arranged to entrance pressure so they have a lot of knowledge and informations to it, but for real they don't have any activity or actions on it. Through this research, we felt that, we should correct students with wrong understanding on controlling weight and wrong knowledge. Also we suggest to make an activity program for this.

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