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Comparison between Bacterial Culture Method and Multiplex PCR for Identification of Fusobacterium nucleatum and Actinobacillus actinomycetemcomitans from the Dental Plaques (치면세균막내의 Fusobacterium nucleatum과 Actinobacillus actinomycetemcomitans의 동정을 위한 세균배양법 및 Multiplex PCR법의 비교)

  • Kim, Hwa-Sook;Lim, Sun-A
    • Journal of dental hygiene science
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    • v.9 no.2
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    • pp.249-255
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    • 2009
  • This study was carried out for the purpose of comparing bacterial culture method, single PCR, and multiplex PCR for identification of F. nucleatum and A. actinomycetemcomitans in subgingival plaque of adult periodontitis. Targeting 20 patients with adult periodontitis, the subgingival plaque was collected in teeth, respectively, for #16, #36, #44. A bacillus was cultivated by painting it over the solid selective media of F. nucleatum and A. actinomycetemcomitans. Bacterial species were detected in 0 tooth with 12 pieces, respectively. Through single PCR and multiplex PCR, the positive reaction was indicated in 43 teeth with 45 pieces, respectively, as for F. nucleatum, and in 1 tooth with 4 pieces, respectively, as for A. actinomycetemcomitans. In the comparative analysis between bacterial identification methods. F. nucleatum showed the more statistically significant difference(p=0.0(0) in comparison between single PCR and multiplex PCR. Even A. actinomycetemcomitans was indicated significantly(p=0.067) in a case that is based on 0.1 in significant level in the comparison between single PCR and multiplex PCR. In conclusion, as a result of comparing the bacterial identification methods, the detection frequency was indicated to be higher in PCR than in bacterial culture method. Single PCR and multiplex PCR showed the mutually similar detection frequency. Accordingly, given thinking of economic efficiency, quickness, and reduction in labor force, it is thought to be more efficient method to use single PCR as the bacterial identification method.

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The Critical Pigment Volume Concentration Concept for Paper Coatings: II. Later-Bound Clay; Ground Calcium Carbonate, and Clay- carbonate Pigment Coatings

  • Lee, Do-Ik
    • Journal of Korea Technical Association of The Pulp and Paper Industry
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    • v.34 no.5
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    • pp.18-38
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    • 2002
  • A previous study on the model coatings based on latex-bound plastic pigment coatings (1) has been extended to latex-bound No. 1 clay, ultra-fine ground calcium carbonate (UFGCC), and clay-carbonate pigment mixture coatings, which are being widely used in the paper industry. The latex binder used was a good film-forming, monodisperse S/B latex or 0.15$\mu\textrm{m}$. No. 1 clay was representative of plate-like pigment particles, whereas UFGCC was of somewhat rounded rhombohedral pigment particlel. Both of them had negatively skewed triangular particle size distributions having the mean particle suet of 0.7${\mu}{\textrm}{m}$ and 0.6$\mu\textrm{m}$, respectively. Their packing volumes were found to be 62.5% and 657%, respectively. while their critical pigment volume concentrations (CPVC's) were determined to be 52.7% and 50.5% ( average of 45% caused by the incompatibility and 55.9% extrapolated) by coating porosity, respectively. Each pigment/latex coating system has shown its unique relationship between coating properties and pigment concentrations, especially above its CPVC. Notably, the clay/latex coating system hat shown higher coating porosity than the UFGCC/latex system at high pigment concentrations above their respective CPVC's. It was also found that their coating porosity and gloss were inter-related to each other above the CPVC's, as predicted by the theory. More interestingly, the blends of these two pigments have shown unique rheological and coating properties which may explain why such pigment blends are widely used in the industry. These findings have suggested that the unique structure of clay coatings and the unique high-shear rheology of ground calcium carbonate coatings can be judiciously combined to achieve superior coatings. Importantly, the low-shear viscosity of the blends was indicative of their unique packing and coating structure, whereas their high-shear rheology was represented by a common mixing rule, i.e., a viscosity-averaging. Transmission and scanning electron and atomic force microscopes were used to probe the state of pigment / latex dispersions, coating surfaces, freeze fractured coating cross-sections, and coating surface topography. These microscopic studies complemented the above observations. In addition, the ratio, R, of CPVC/(Pigment Packing Volume) has been proposed as a measure of the binder efficiency for a given pigment or pigment mixtures or as a measure of binder-pigment interactions. Also, a mathematical model has been proposed to estimate the packing volumes of clay and ground calcium carbonate pigments with their respective particle size distributions. As well known in the particle packing, the narrower the particle size distributions, the lower the packing volumes and the greater the coating porosity, regardless of particle shapes.

Effect of Dietary Supplementation with Dried Powder of Pumpkin on Quality Characteristics of Pork (호박분말 급여가 돈육의 품질 특성에 미치는 영향)

  • Jin, Sang-Keun;Yang, Han-Sul
    • Journal of agriculture & life science
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    • v.46 no.2
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    • pp.91-99
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    • 2012
  • This study was carried out to compare the quality properties of pork loin from pigs fed different levels of pumpkin (Cucurbita spp.) powder. Twenty female pigs (Landrace${\times}$Yorkshire${\times}$Duroc) were fed either a control (commercial diet, based on corn and soybean meal) or the control diet supplemented with 2, 5 and 7% of pumpkin powder for 30 days. Dietary supplementation with pumpkin powder resulted in significantly higher moisture and fat contents in pork loin compared to the control (p<0.05). Dietary supplementation with pumpkin powder led to increased redness, pH and decreased shear force value and cholesterol levels in pork loin (p<0.05). In fatty acid composition, dietary supplementation with 2% or 5% pumpkin powder increased the palmitoleic acid (C16:1) in pork loin. These data suggest that supplementing pig diets with pumpkin powder can produce pork loin with low cholesterol levels and can enhance quality properties because pork loin fed a pumpkin powder-supplemented diet had better moisture, fat contents and tenderness.

Is Civic Service the Real Antipode of Volunteer Work? - Focusing on AmeriCorps and Senior Corps in the United States (시민서비스는 자원봉사의 대척점에 있는가? - 미국의 AmeriCorps와 Senior Corps를 중심으로)

  • Ji, Eun-Jeong
    • Korean Journal of Social Welfare Studies
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    • v.45 no.2
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    • pp.31-63
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    • 2014
  • Recently interest in civic engagement and civic service is increasing. However, in Korea, few studies have focused on civic service. This might be on account of general awareness that civic service is fundamentally different from the basic spirit of voluntary work, while interest in paid volunteer work is on the rise. Thus, it is necessary to examine whether civic service is the real antipode of volunteer work. Under this kind of critical viewpoint, this study aims to analyze civic service based on the attributes of voluntary work and civic service. The major findings are as follows. Firstly, contrary to common belief, civic service has not been established to go beyond the principle of voluntary and unpaid characteristics of volunteer work. Rather, some voluntary work has broken out of principle of voluntary activities. Secondly, civic service and volunteer work might be characterized as different not due to spontaneity and unpaid service but the structural characteristics of the goal, continuity and formality. Furthermore, the reason for the soft landing of civic service in the United States is not because they have supported the reimbursement of expenses and the provision of stipends. Rather, it is because their long-term activities have promoted real community development for the purpose of finding solutions to social problems, and they have derived a sense of pride and satisfaction from social recognition and rewards for their contributions.

The Analysis on the Export and Import Performance in the Korea Content Industry of the Korea-USA FTA (한·미 FTA의 콘텐츠산업 수출입 효과 분석)

  • Jung, Sang-chul;Ko, Jeong-Min
    • Review of Culture and Economy
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    • v.21 no.2
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    • pp.29-51
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    • 2018
  • The Korea-USA FTA was activated in 2012 and evaluated highly on market-opening among FTA agreements which the Korea government has signed. This paper empirically examines the impact of the Korea-USA FTA on the Korea content industry trade performance with USA. Using ANOVA analysis, the study tested primarily whether there are difference in content exports and imports in a korea-US trade between before and after of Korea-USA FTA in 2012, the year of the entry into force of Korea-USA FTA. Using content industry exports and import data over the period 2007-2015, we find that Korea content export to US after 2012 is significantly different from one before 2012, and there is no difference in import. Based on a regression analysis, we also tested the impact size of Korea content export performance in Korea-USA FTA. The result is that coefficient of FTA dummy variable is not significant, meaning that even though there are some difference in korea content export to US between before and after of Korea-USA FTA, the Korea-USA FTA agreement itself is not a key factor which increase the Korea content export volume to USA market.

A positive interpretation of Louis Lavelle for the Presence of Evil and Pain, and its Limitations - Focusing on evil and suffering - (루이 라벨의 '악의 현존과 고통'에 대한 긍정적인 해석 그리고 그 한계 - 『악과 고통들』을 중심으로 -)

  • Lee, Myoung-gon
    • Journal of Korean Philosophical Society
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    • v.146
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    • pp.163-192
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    • 2018
  • In the philosophy of Louis Labelle, the subject of "good and evil", which is rarely discussed in modern philosophy, emerges as a very important concept. In his reasoning, the notion of evil starts from a passive stipulation of the Scholastic philosophy of "the lack of being", but he defined the evil, which actually exists in the world with more positive sense as a willing force destroying "life and values". In his reasoning that defines "the presence of evil" as "le scandale du monde", all humans have the possibility of evil as an attitude of will, and the presence of evil in the world and in human society is inevitable. On the other hand, because the outcome of evil appears as physical and mental "pain", the human's attitude toward pain, which attempts to deny suffering, induces a moral will to overcome this evil. The moral anguish, which is the "internal suffering" that we have in front of the presence of evil, makes us conscious of becoming a "moral being", and people become self-satisfied here. Although painful, self-satisfaction and happiness about becoming a moral being is the only occasion and motive for man to overcome this by opposing the presence of evil. In other words, Lavelle's thoughts for good and evil are based on "moral psychologism", and the "coherence of psychological horizons and ethical horizons" between rejection of suffering and moral agony enables "ethical optimism" that man constantly overcomes evil and produces good. This is clearly an advanced modern application of scholasticism on the concept of evil.

Effect of visual marbling levels in pork loins on meat quality and Thai consumer acceptance and purchase intent

  • Noidad, Sawankamol;Limsupavanich, Rutcharin;Suwonsichon, Suntaree;Chaosap, Chanporn
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.12
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    • pp.1923-1932
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    • 2019
  • Objective: We investigated visual marbling level (VML) influence on pork loin physicochemical traits, consumer palatability responses, VML liking, purchase intent, and their relationships. Methods: For each of five slaughtering dates, at 24-h postmortem, nine paired Duroc castrated male boneless Longissimus dorsi (LD) muscles were categorized into low (LM, score 1 to 2, n = 3), medium (MM, score 3 to 4, n = 3), and high (HM, score 5 to 6, n = 3) VML. Meat physicochemical quality traits and consumer responses (n = 389) on palatability and VML liking, and purchase intent were evaluated. The experiment was in randomized complete block design. Analysis of variance, Duncan's multiple mean comparisons, and correlation coefficients were determined. Results: VML correspond to crude fat (r = 0.91, p<0.01), but both were reversely related to moisture content (r = -0.75 and -0.91, p<0.01, respectively). As VML increased, ash (p<0.05) and protein (p = 0.072) decreased, pH and $b^{\star}$ increased (p<0.05), but drip, cooking (p<0.05) and thawing (p = 0.088) losses decreased. Among treatments, muscle fiber diameter, sarcomere length, total and insoluble collagen contents, $L^{\star}$, and $a^{\star}$ did not differ (p>0.05). Compared to the others, HM had lower collagen solubility percentage (p<0.05), but similar (p>0.05) Warner-Bratzler shear force (WBSF). No differences (p>0.05) were found in juiciness, overall flavor, oiliness, and overall acceptability, but HM was more tender (p<0.05) than the others. Based on VML, consumers preferred MM to HM (p<0.05), while LM was similar to MM and HM (p>0.05). Corresponding to VML preference (r = 0.45, p<0.01), consumers (83%) would (p<0.01) definitely and probably buy MM, over LM (74%), and HM (68%), respectively. Conclusion: Increasing VML in pork LD altered its chemical composition, slightly increased pH, and improved water holding capacity, thereby improving its tenderness acceptability. Marbling might reduce chewing resistance, as lower collagen solubility in HM did not impact tenderness acceptability and WBSF. While HM was rated as most tender, consumers visually preferred and would purchase MM.

Analysis of related words for each private security service through collection of unstructured data

  • Park, Su-Hyeon;Cho, Cheol-Kyu
    • Journal of the Korea Society of Computer and Information
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    • v.25 no.6
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    • pp.219-224
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    • 2020
  • The purpose of this study is mainly to provide theoretical basis of private security industry by analyzing the perception and flow of private security from the press-released materials according to periodic classification and duties through 'Big Kinds', a website of analyzing news big data. The research method has been changed to structured data to allow an analysis of various scattered unstructured data, and the keywords trend and related words by duties of private security were analyzed in growth period of private security. The perception of private security based on the results of the study was exposed a lot by the media through various crimes, accidents and incidents, and the issues related permanent position. Also, it tended to be perceived as a simple security guard, not recognized as the area of private security, and judging from the high correlation between private security and police, it was recognized not only as a role to assist the police force, but also as a common agent in charge of the public peace. Therefore, it should objectively judge the perception of private security, and through this, it is believed that it should be a foundation for recognizing private security as a main agent responsible for the safety of the nation and maintaining social orders.

Convergence Comparison of Linear Oscillating Electric Machines (리니어 오실레이팅 전기기기의 비교 연구)

  • Jeong, Sung-In;Eom, Sang In
    • Journal of the Korea Convergence Society
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    • v.12 no.12
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    • pp.273-280
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    • 2021
  • This paper presents the results of study of linear oscillating electric machine; Cartesian, cylindrical type with permanent magnet, flux reversal, cylindrical reluctance, and transverse flux type. The focus of the work is the suggestion of the characteristics and design process of propose topology, respectively. First of all, there are five types of the proposed to this study on the basis of the existing literatures; Cartesian type, cylindrical type, flux reversal type, cylindrical reluctance type, and transverse flux type. All topology is achieved using equivalent magnetic circuit considering leakage elements as initial modeling. Cartesian type is investigated by number of phases and number of pole pairs using optimal process. A cylindrical type is described by number of phases and displacement of stroke. The flux reversal type is proposed based on the symmetrical and non symmetrical stator cores of the surface mounted PMs mover, and non slanted PMs and slanted PMs of the flux concentrating PMs mover. A cylindrical reluctance type is studied by the shape of mover teeth in geometric aspect to reduce force ripple and increase magnetic flux. A transverse flux type is considered by dividing the transverse flux electric excited and the transverse flux permanent magnet excited. It is significant that the study gives a design rules and features of linear oscillating electric machine.

Biomechanical Analysis of Walking and Running after a Surgically Repaired Achilles Tendon Rupture (아킬레스건 파열 수술 후 걷기 및 달리기 운동역학적 분석)

  • Heo, Jeong;Park, Sang-Kyoon
    • Korean Journal of Applied Biomechanics
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    • v.31 no.4
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    • pp.241-248
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    • 2021
  • Objective: The purpose of this study was to investigate the difference in muscle strength, kinematics, and kinetics between injured and non-injured sides of the leg after Achilles Tendon Rupture surgery during walking and running. Method: The subjects (n=11; age = 30.63 ± 5.69 yrs; height = 172.00 ± 4.47 cm; mass = 77.00 ± 11.34 kg; time lapse from surgery = 29.81 ± 10.27 months) who experienced Achilles Tendon Rupture (ATR) surgery participated in this study. The walking and running trials were collected using infrared cameras (Oqus 300, Qualisys, Sweden, 100 Hz) on instrumented treadmill (Bertec, U.S.A., 1,000 Hz) and analyzed by using QTM (Qualisys Track Manager Ver. 2.15; Qualisys, U.S.A). The measured data were processed using Visual 3D (C-motion Inc., U.S.A.). The cutoff frequencies were set as 6 Hz and 12 Hz for walking and running kinematics respectively, while 100 Hz was used for force plate data. Results: In ATR group, muscle strength there were no difference between affected and unaffected sides (p> .05). In kinematic analysis, subjects showed greater ROM of knee joint flexion-extension in affected side compared to that of unaffected side during walking while smaller ROM of ankle dorsi-plantar and peak knee flexion were observed during running (p< .05). In kinetic analysis, subjects showed lower knee extension moment (running at 2.2 m/s) and positive ankle plantar-flexion power (running at 2.2 m/s, 3.3 m/s) in affected side compared to that of unaffected side (p< .05). This lower positive ankle joint power during a propulsive phase of running is related to slower ankle joint velocity in affected side of the subjects (p< .05). Conclusion: This study aimed to investigate the functional evaluation of the individuals after Achilles tendon rupture surgery through biomechanical analysis during walking and running trials. Based on the findings, greater reduction in dynamic joint function (i.e. lower positive ankle joint power) was found in the affected side of the leg compared to the unaffected side during running while there were no meaningful differences in ankle muscle strength and walking biomechanics. Therefore, before returning to daily life and sports activities, biomechanical analysis using more dynamic movements such as running and jumping trials followed by current clinical evaluations would be helpful in preventing Achilles tendon re-rupture or secondary injury.