• Biomolecules & Therapeutics
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• 제31권3호
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• pp.350-358
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• 2023

Hand-foot-and-mouth disease (HFMD) is a viral infectious disease that occurs in children under 5 years of age. Its main causes are coxsackievirus (CV) and enterovirus (EV). Since there are no efficient therapeutics for HFMD, vaccines are effective in preventing the disease. To develop broad coverage against CV and EV, the development of a bivalent vaccine form is needed. The Mongolian gerbil is an efficient and suitable animal model of EV71 C4a and CVA16 infection used to investigate vaccine efficacy following direct immunization. In this study, Mongolian gerbils were immunized with a bivalent inactivated EV71 C4a and inactivated CVA16 vaccine to test their effectiveness against viral infection. Bivalent vaccine immunization resulted in increased Ag-specific IgG antibody production; specifically, EV71 C4a-specific IgG was increased with medium and high doses and CVA16-specific IgG was increased with all doses of immunization. When gene expression of T cell-biased cytokines was analysed, Th1, Th2, and Th17 responses were found to be highly activated in the high-dose immunization group. Moreover, bivalent vaccine immunization mitigated paralytic signs and increased the survival rate following lethal viral challenges. When the viral RNA content was determined from various organs, all three doses of bivalent vaccine immunization were found to significantly decrease viral amplification. Upon histologic examination, EV71 C4a and CVA16 induced tissue damage to the heart and muscle. However, bivalent vaccine immunization alleviated this in a dose-dependent manner. These results suggest that the bivalent inactivated EV71 C4a/CVA16 vaccine could be a safe and effective candidate HFMD vaccine.

• 과학기술학연구
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• 제18권2호
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• pp.307-342
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• 2018

이 글은 지난 2010년-2011년 사이에 한국사회를 충격으로 몰아넣었던 구제역의 확산과정에서 질병진단방식을 둘러싼 논쟁에 대해서 논의하게 될 것이다. 당시 구제역 방역과 질병진단 방식을 둘러싸고 다양한 사회집단들 사이에서 치열한 논쟁이 있었으며 그 중심에는 휴대용 항체/항원 간이진단키트가 논쟁의 대상이 되었다. 이 항체/항원 간이진단키트의 효용성으로 부터 사용권한까지 논쟁은 구제역 확산기간 동안 지속되었으며 특히 중앙정부를 중심으로 하는 방역당국과 지방정부와 시민단체들은 이 간이진단키트의 사용권과 병성감정권한을 둘러싸고 논쟁을 벌이게 된다. 이 논문은 항체/항원 간이진단키트를 둘러싼 의미부여방식과 기술적인 판단에 대한 각기 다른 해석방식을 설명하기 위해 "경계물(boundary objects)" 개념을 사용하여 설명할 것이다. 특히 경계물의 구성방식과 각기 다른 사회집단이 이 경계물을 포함하거나 배제하는 과정을 경계만들기(boundary making)로 해석하면서 경계물과 경계만들기 과정의 상호작용과 협상과정을 추적하게 될 것이다. 이를 통해 의학기술적 대상물인 간이진단키트가 경계물로 구성될 뿐 아니라 이를 둘러싼 사회집단의 경계만들기는 상호작용을 통해 구성되는 공구성의 과정이라는 것을 보이게 될 것이다.

• 한국동물위생학회지
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• 제44권1호
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• pp.15-19
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• 2021

Senecavirus A (SVA), previously known as Seneca Valley virus, can cause vesicular disease and neonatal losses in pigs that is clinically indistinguishable from foot-and-mouth disease virus (FMDV). After the first case report in Canada in 2007, it had been restrictively identified in North America including United States. But, since 2015, SVA emerged outside North America in Brazil, and also in several the Asian countries including China, Thailand, and Vietnam. Considering the SVA occurrence in neighboring countries, there has been a high risk that Korea can be introduced at any time. In particular, it is very important in terms of differential diagnosis in the suspected case of vesicular diseases in countries where FMD is occurring. So far, several different molecular detection methods for SVV have been published but not validated as the reference method, yet. In this study, seven different molecular methods for detecting SVA were evaluated. Among them, the method by Flowler et al, (2017) targeted to 3D gene region with the highest sensitivity and no cross reaction with other vesicular disease agents including FMDV, VSV and SVD, was selected and applied further to antigen surveillance of SVA. A total of 245 samples of 157 pigs from 61 farms submitted for animal disease diagnose nationwide during 2018 were tested all negative. In 2018, no sign of SVA occurrence have been confirmed in Korea, but the results of the surveillance for SVA needs to be continued and accumulated at a high risk of SVA in neighboring countries.

• 대한수의학회지
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• 제49권3호
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• pp.221-229
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• 2009

Foot-and-mouth disease (FMD) is the most contagious disease of mammals. The use of inactivated vaccine can be chosen to prevent or control FMD. However, vaccination against one serotype of FMDV doses not cross-protect against other serotypes and may not protect fully against some strains of the same serotype. Appropriate selection of vaccine strain is an important element in the control of FMD. The immunity of vaccine antigens should be matched against newly circulating viruses. The phylogenetic analysis of serotype Asia1 reported from China, Mongolia, North Korea and Russia since 2005 shows that they are all classified into genetic group V, but the strain, Asia1/Shamir (ISR/89) which have been used as a vaccine strain in Korea, is clustered into different genetic group. So, in this study the serological relationship between the isolate (Asia1/MOG/05; MOG) and the Shamir strain was determined by ELISA and virus neutralization test. Even though the matching value of the virus (MOG) against the vaccinated sera in target animals was not so high, the vaccinated animals elicited antibodies enough for protection after vaccinated once or twice. Conclusively, we suggest that the vaccine containing Asia1/Shamir antigen could protect the genetic group V strains circulating in East Asia currently if vaccinated twice or the more.

• 한국식품영양학회지
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• 제19권2호
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• pp.126-142
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• 2006

A survey was conducted to investigate the relationship between knowledge of perceived food safety and actual food purchase practices among parents of children under the age of seven (N=309) in 2005. The seventeen following perceived food safety items were included in the survey (bovine spongiform encephalopathy, foot and mouth disease, pathogenic avian influenza, swine fever, allergens, GMO, irradiated food, food poisoning bacteria, endocrine disrupter, antibiotics, food additives, carcinogen, natural toxin, agrichemical residues, place of origin, cultivation methods, and heavy metal) Most parents were highly concerned about investigating all food safety topics. Up to 90.0% of the participants recognized the importance of food safety. The public concern has caused a considerable decline in the demand for food afterreceiving news about food safety through mass media, Also, the majority of subjects required correct food safety information.

• 축산식품과학과 산업
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• 제8권1호
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• pp.60-61
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• 2019

• 대한수의사회지
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• 제33권5호
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• pp.258-277
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• 1997

우리나라와 축산물(돼지고기) 등의 무역이 활발한 동북아의 국가인 대만은 1930년 구제역의 최종 발생보고 이후 약 47년간 비발생 상태를 유지해 왔었다. 1997년 3월 20일 오전 11:00경 대만에 구제역이 발생하였다는 대만정부의 공식발표에 따라 우리나라에서는 동일자로 대만산 우제류 동물 및 그 생산물의 수입금지를 전면적으로 실시함과 동시에 `97. 2. 17. 이후 도축되어 우리나라에 수입된 돼지고기에 대하여 전량 반송조치토록 수입자에게 통보하는 등 구제역의 국내유입 차단을 위한 정부차원의 여러가지 조치 등을 지속적으로 취하고 있다. 이에 필자들은 미국의 농무성 동식물검역청(USDA, APHIS)에서 구제역 발생 및 구제역바이러스의 전파위험도를 분석한 자료 (Foot-and-Mouth Disease : Soures of Outbreaks and Hazard Categorization of Modes of Virus Transmission, December. 1994, USDA : APHIS : VS, Centers for Epidemiology and Animal Health)를 소개함으로써 구제역바이러스의 국내 유입방지 대책을 위한 기초자료로 제시하고자 한다.

• Journal of Veterinary Science
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• 제24권1호
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• pp.15.1-15.13
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• 2023

Background: Inactivated vaccines are limited in preventing foot-and-mouth disease (FMD) due to safety problems. Recombinant virus-like particles (VLPs) are an excellent candidate for a novel vaccine for preventing FMD, given that VLPs have similar immunogenicity as natural viruses and are replication- and infection-incompetent. Objectives: The 3C protease and P1 polyprotein of type O FMD virus (FDMV) was expressed in yeast Hansenula polymorpha to generate self-resembling VLPs, and the potential of recombinant VLPs as an FMD vaccine was evaluated. Methods: BALB/c mice were immunized with recombinant purified VLPs using CpG oligodeoxynucleotide and aluminum hydroxide gel as an adjuvant. Cytokines and lymphocytes from serum and spleen were analyzed by enzyme-linked immunosorbent assay, enzyme-linked immunospot assay, and flow cytometry. Results: The VLPs of FMD were purified successfully from yeast protein with a diameter of approximately 25 nm. The immunization of mice showed that animals produced high levels of FMDV antibodies and a higher level of antibodies for a longer time. In addition, higher levels of interferon-γ and CD4⁺ T cells were observed in mice immunized with VLPs. Conclusions: The expression of VLPs of FMD in H. polymorpha provides a novel strategy for the generation of the FMDV vaccine.

• Clinical and Experimental Vaccine Research
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• 제13권2호
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• pp.132-145
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• 2024

Purpose: Enterovirus 71, a pathogen that causes hand-foot and mouth disease (HFMD) is currently regarded as an increasing neurotropic virus in Asia and can cause severe complications in pediatric patients with blister-like sores or rashes on the hand, feet, and mouth. Notwithstanding the significant burden of the disease, no authorized vaccine is available. Previously identified attenuated and inactivated vaccines are worthless over time owing to changes in the viral genome. Materials and Methods: A novel vaccine construct using B-cell derived T-cell epitopes from the virulent polyprotein found the induction of possible immune response. In order to boost the immune system, a beta-defensin 1 preproprotein adjuvant with EAAAK linker was added at the N-terminal end of the vaccine sequence. Results: The immunogenicity of the designed, refined, and verified prospective three-dimensional-structure of the multi-epitope vaccine was found to be quite high, exhibiting non-allergenic and antigenic properties. The vaccine candidates bound to toll-like receptor 3 in a molecular docking analysis, and the efficacy of the potential vaccine to generate a strong immune response was assessed through in silico immunological simulation. Conclusion: Computational analysis has shown that the proposed multi-epitope vaccine is possibly safe for use in humans and can elicit an immune response.

• Journal of Yeungnam Medical Science
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• 제30권1호
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• pp.58-61
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• 2013

Enterovirus commonly causes neurologic diseases (aseptic meningitis, encephalitis, etc.), hand-foot-mouth disease, herpangina, and acute hemorrhagic conjunctivitis. However, it rarely causes pneumonia in immunocompetent adults. In Korea, no case has been reported about pneumonia caused by enterovirus in healthy adults. We can cite the case of a 20-year-old woman who presented severe community-acquired pneumonia caused by enterovirus. The diagnosis was based on reverse transcriptase polymerase chain reaction (RT-PCR) of a respiratory specimen.