• Title/Summary/Keyword: food science and technology

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Inhibitory Effect of Ecklonia cava Extracts against Lipase Activity and Stability Effect of Temperature and pH on Their Activity (감태 추출물이 지니는 Lipase 저해활성의 열 및 pH 안정성)

  • Jung, Ji-Yeon;Kim, Koth-Bong-Woo-Ri;Lee, Chung-Jo;Kwak, Ji-Hee;Kim, Min-Ji;Kim, Dong-Hyun;SunWoo, Chan;Kim, Tae-Wan;Ahn, Dong-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.40 no.7
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    • pp.969-974
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    • 2011
  • This study was performed to investigate the inhibitory activity of Ecklonia cava (EC) against lipase and the stability of this activity under various heat and pH conditions. As a result, EC ethanol extract showed lipase inhibitory activity of 59, 34 and 19% at concentrations of 5, 2.5 and 1 mg/mL, whereas the water extract showed low inhibitory activity at all concentrations compared to that of the ethanol extracts. In a heat and pH stability test, the inhibitory activity of the EC ethanol extract increased with heat treatment at $121^{\circ}C$ for 15 min compared with the control and was stable in the pH range of 2~10. Therefore, the EC ethanol extract could be useful as a natural anti-obesity agent.

Effect of Immersion Solution Composition and Duration on the Re-moisturization of Dried Flying Fish Cypselurus agoo Roe (날치(Cypselurus agoo) 건조 어란의 수분함량 복원을 위한 침지액의 효과)

  • Seul-Ki Park;Yeon-Ju Sim;Jeong-Bin Jo;Kyung-Jin Cho;Du-Min Jo;So-Yeon Noh;Ye-Bin Jang;Mi-Ru Song;Young-Mog Kim
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.56 no.3
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    • pp.347-351
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    • 2023
  • Flying fish Cypselurus agoo roe is a popular food ingredient due to its abundant nutrition, unique texture, and flavor. It contains high levels of polyunsaturated fatty acids, protein, and minerals such as calcium, phosphorus, potassium, and vitamins (A, B1, B2, B6, and niacin), making it a highly utilized food ingredient. Additionally, it is widely used as a raw material for food and cooking in several countries. After harvesting, flying fish roe is dried and transported to processing plants, where it performed a re-moisturizing process. However, the conventional re-moisturizing process is time-consuming and results in some loss of quality. Thus, this study investigates the conditions required for the re-moisturization of flying fish roe including the composition of the immersion solution, immersion time, and conditions that could minimize damage to the roe. The results suggest a reasonable composition of immersion solution to minimize damage to flying fish roe and the most appropriate immersion time for re-moisturization. These results may provide valuable insights for the industry to enhance the quality of re-moisturized flying fish roe, leading to an increase in its market value and demand.

Development of Detection Method for Cyclomaltodextrinase Family Genes using Degenerate PCR Primers

  • Oh, Su-Won;Jang, Myoung-Uoon;Jeong, Chang-Ku;Yuk, Jeong-Bin;Park, Jung-Mi;Park, Kwan-Hwa;Kim, Tae-Jip
    • Food Science and Biotechnology
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    • v.15 no.6
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    • pp.967-974
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    • 2006
  • Cyclomaltodextrinases (CDases), maitogenic amylases, and neopullulanases share highly conserved primary structures and similar characteristics, and are thus classified into the same family. BLAST search has showed that a variety of bacterial strains harbor putative CDase family genes with several well-conserved motif amino acid sequences. In this study, four degenerate polymerase chain reaction (PCR) primer sets were designed for the detection of CDase genes, on the basis of their highly conserved amino acid blocks (WYQIFP, DGWRLD, LGSHDT, and KCMVW). The PCR detection conditions were optimized and the detection specificity of each for the primer sets was tested against the genomic DNAs isolated from 23 different Bacillus-associated species. Consequently, all tested primer sets evidenced successful amplification of specific PCR products in length, which share 55-98% amino acid sequence identity with known and putative CDases. The primers developed herein, therefore, can be applied for the easy and efficient detection and isolation of CDase family genes for the modification of functional food carbohydrates.

Validation and measurement uncertainty of HPLC-UV method for quercetin quantification in various foods

  • Seo, Eunbin;Lim, Suji;Yun, Choong-In;Shin, Jae-Wook;Kim, Young-Jun
    • Korean Journal of Food Science and Technology
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    • v.53 no.6
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    • pp.682-687
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    • 2021
  • The purpose of this study was to validate a high-performance liquid chromatography (HPLC) method for the quantitative analysis of quercetin in various foods. The method was based on HPLC-UV (360 nm). The method was validated using candy, beverage, and sausage which were tested for specificity, linearity, limit of detection (LOD), limit of quantification (LOQ), precision, and accuracy, and the measurement uncertainty was assessed. Matrix-matched calibration was also applied. The calibration curves (0.5-50 mg/L) showed good linearity (r2≥0.9998). LOD and LOQ ranged from 0.15 to 0.31 mg/kg and from 0.44 to 0.93 mg/kg, respectively. The average accuracy and precision at 0.5, 2.5, and 10 mg/kg ranged from 84.3 to 102.0% and 0.7 to 3.0 relative standard deviation (RSD%), respectively. This study confirmed the applicability of the proposed method by applying it to commercial products, such as teas and beverages. Thus, the proposed analytical method is suitable for quantifying quercetin in various foods.

Extending Raw Pacific Oyster Crassostrea gigas Shelf-life by Addition of the Natural Food Additives (천연 추출물 첨가에 의한 생굴(Crassostrea gigas)의 식품학적 품질 유지)

  • DoKyung Oh;Do-Ha Lee;Du-Min Jo;Kyung-Jin Cho;Seul-Ki Park;Yeon-Ju Sim;Jeong-Bin Jo;Jae-Ho Woon;Young-Mog Kim
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.56 no.6
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    • pp.810-817
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    • 2023
  • Oysters are a highly consumed seafood throughout Korea, but they have a short shelf life because they support rapid microbial growth due to their of high moisture content and fragile muscle tissue. We examined natural food additives including lactic acid bacteria fermentation powder, rosemary extract, and lemon juice for their ability to preserve raw oyster Crassostrea gigas quality. Samples were stored at 4℃, and microbiological and physicochemical analyses were conducted. Among the natural additives tested, lemon juice was the most effective. Lemon juice was thus applied at different concentrations (50-300 ppm) to quantitatively assess its effect on total viable cell count, pH, glycogen, soluble protein, and turbidity. 200 ppm was confirmed to be optimal, and is projected to extend shelf life by 2 days compared to the control group.

Characterization and Comparative Evaluation of Milk Protein Variants from Pakistani Dairy Breeds

  • Yasmin, Iqra;Iqbal, Rabia;Liaqat, Atif;Khan, Wahab Ali;Nadeem, Muhamad;Iqbal, Aamir;Chughtai, Muhammad Farhan Jahangir;Rehman, Syed Junaid Ur;Tehseen, Saima;Mehmood, Tariq;Ahsan, Samreen;Tanweer, Saira;Naz, Saima;Khaliq, Adnan
    • Food Science of Animal Resources
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    • v.40 no.5
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    • pp.689-698
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    • 2020
  • The aim of study was to scrutinize the physicochemical and protein profile of milk obtained from local Pakistani breeds of milch animals such as Nilli-Ravi buffalo, Sahiwal cow, Kajli sheep, Beetal goat and Brela camel. Physicochemical analysis unveiled maximum number of total solids and protein found in sheep and minimum in camel. Buffalo milk contains the highest level of fat (7.45%) while camel milk contains minimum (1.94%). Ash was found maximum in buffalo (0.81%) and sheep (0.80%) while minimum in cow's milk (0.71%). Casein and whey proteins were separated by subjecting milk to isoelectric pH and then analyzed through sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). The results showed heterogeneity among these species. Different fractions including αS1, αS2, κ-casein, β-casein and β-lactoglobulen (β-Lg) were identified and quantitatively compared in all milk samples. Additionally, this electrophoretic method after examining the number and strength of different protein bands (αS1, αS2, β-CN, α-LAC, BSA, and β-Lg, etc.), was helpful to understand the properties of milk for different processing purposes and could be successfully applied in dairy industry. Results revealed that camel milk was best suitable for producing allergen free milk protein products. Furthermore, based on the variability of milk proteins, it is suggested to clarify the phylogenetic relationships between different cattle breeds and to gather the necessary data to preserve the genetic fund and biodiversity of the local breeds. Thus, the study of milk protein from different breed and species has a wide range of scope in producing diverse protein based dairy products like cheese.