• BMB Reports
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• 제29권6호
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• pp.519-524
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• 1996

Peptidylprolyl cis-trans isomerase (PPlase) catalyzes the cis-trans isomerization of prolyl peptide and facilitates the folding of cellular proteins and peptides. PPlase consists of two distinct immunophilins, each specifically binding to the immunosupressive drug cyclosporin A (CsA) or FK506, respectively. A PPlase was isolated and partially purified from porcine spleen. The molecular weight of porcine spleen PPlase was determined to be ~14,000 on the basis of SDS-PAGE. The purified enzyme was strongly inhibited by FK506, but not by CsA. The inhibition constant and the true concentration of enzyme preparations were determined by active site titration using the tight binding inhibitor FK506: $K_{i}=18.7$ nM and $E_{t}=172$ nM. The equilibrium ratio of conformer. [cis]/[trans], of prolyl peptide substrates (N-Suc-Ala-Xaa-Pro-Phe-p-NA) in anhydrous trifluoroethanol/LiCl solvent system varied from 0.24 to 0.85 depending on the nature of Xaa. Overall. in this solvent-salt system, the populations of the cis conformer of substrates in equilibrium are higher than in an aqueous solution so that the substantial error caused by high background absorption can be reduced. The reactivities of porcine spleen PPlase are shown to be highly sensitive to changes in the structure of substrates. Thus, $k_{cat}/K_m$ value for the most reactive substrate (Xaa Leu) is $4.007+10^{6}M^{1}s^{1}$ and, is 2,636 fold higher than that for the least reactive peptide substrate tested, Xaa=Glu.

• BMB Reports
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• 제55권10호
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• pp.488-493
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• 2022

The specific pair of heat shock protein 70 (Hsp70) and Hsp40 constitutes an essential molecular chaperone system involved in numerous cellular processes, including the proper folding/refolding and transport of proteins. Hsp40 family members are characterized by the presence of a conserved J-domain (JD) that functions as a co-chaperone of Hsp70. Tumorous imaginal disc 1 (Tid1) is a tumor suppressor protein belonging to the DNAJA3 subfamily of Hsp40 and functions as a co-chaperone of the mitochondrial Hsp70, mortalin. In this work, we performed nuclear magnetic resonance spectroscopy to determine the solution structure of JD and its interaction with the glycine/phenylalanine-rich region (GF-motif) of human Tid1. Notably, Tid1-JD, whose conformation was consistent with that of the DNAJB1 JD, appeared to stably interact with its subsequent GF-motif region. Collectively with our sequence analysis, the present results demonstrate that the functional and regulatory mode of Tid1 resembles that of the DNAJB1 subfamily members rather than DNAJA1 or DNAJA2 subfamily proteins. Therefore, it is suggested that an allosteric interaction between mortalin and Tid1 is involved in the mitochondrial Hsp70/Hsp40 chaperone system.

• 헤리티지:역사와 과학
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• 제55권2호
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• pp.146-165
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• 2022

이 연구는 2021년 경북 유형문화재 <상주 전식 조복 및 공예품 일괄>로 지정된 유물 중 전식(全湜, 1563~1642)의 조복 구성물인 3점(의·상·대대)의 유물을 17~18세기 5건(신경유, 권우, 화산군 이연, 밀창군 이직, 이익정)의 출토 조복 구성물과 문헌자료를 비교하면서 유물의 조형적 특징과 제도와의 차이점을 밝히고자 함에 목적을 두었다. 전식의 조복 구성물 3점은 색상을 확인할 수 있는 최고(最古)의 조복 유물이라는 점에서 사료적 가치가 크다. 붉은 색 능직물로 제작한 상의(上衣)는 아쉽게도 가선이 소실된 상태이지만 흔적이 남아 있는 부분에 대해 기록이나 초상화 등의 자료에 근거하여 흑색 가선[黑緣]을 추측할 수 있었다. 그리고 흑선과 바탕 옷감 사이에 끼우는 흰 장식선은 아직 사용되지 않았음을 확인하였다. 하상(下裳)은 전3폭 후4폭 중 전3폭과 후1폭만 남은 상태이다. 그러나 후4폭의 형태를 유추할 수는 있었다. 주름 흔적으로 볼 때 허리 부분에만 주름을 잡았음을 알 수 있었다. 18세기의 밀창군, 이익정 묘 조복 상은 허리말기에 간격을 두고 연결되어 있으나 신경유, 권우, 화산군 이연 묘 조복 상 등 17세기 조복 상은 전상 아래로 후상이 겹쳐 연결되어 있는 것으로 볼 때 전식의 조복 상 역시 전상 아래에 5cm 전후로 후상을 겹쳐 연결하였을 것으로 추정할 수 있었다. 화산군 이연(李渷, 1647~1702) 이후 흑선과 바탕과의 사이에 백색 장식선을 끼운 뒤 허리부분에서 밑단까지 주름을 잡은 형태였다. 대대는(大帶)는 '경(冂)' 모양인데 소색(素色)의 주(紬)에 녹색 문사(紋紗) 선을 둘러 긴 띠를 만든 후 모서리를 접어 요(腰)와 신(紳)을 만들었다. 18세기 밀창군과 이익정, 정휘량의 조복본 초상화에서 볼 수 있는 대대의 색상과 일치하였다. 18세기 초 화산군 이연 묘 대대 이후는 요와 신 부분을 따로 재단하여 이은 형태였으나 전식의 대대를 포함한 17세기 신경유 묘, 권우 묘의 대대는 긴 띠를 접어 요와 신을 만들었음을 알 수 있었다. 그리고 허리에 매기 위해 양 끝에 가는 끈을 달았다. 전식의 조복 유물은 색상이나 형태면에서 17세기 조복제도를 구체적으로 반영한 것으로 평가할 수 있으며 『국조오례의 서례』·『경국대전』조복제도의간략하거나부정확한기록을보완해주는사료로서의중요한가치를지니고있다고하겠다.

• 펄프종이기술
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• 제47권4호
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• pp.115-126
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• 2015

In this study, we investigated influence of ground calcium carbonate (GCC) modified by washless multilayering of polyelectrolytes on paper quality. Three layers of polyelectrolytes (cationic starch/anionic polyacrylamide/cationic starch) were formed on the surface of GCC using laboratory inline washless polyelectrolytes multilayering system, which was called inline LbL GCC. Base papers were prepared with untreated GCC or inline LbL GCC using a laboratory handsheet former. These handsheets were coated with rod coater, and then printed by black ink. Properties of base paper and fold crack of coated paper were evaluated. Base paper with inline LbL GCC showed much higher mechanical strength in terms of tensile index, strain, internal bond strength, and folding endurance. The fold crack of coated paper with inline LbL GCC occurred more frequently compared to coated paper with untreated GCC. This might be due to highly improved internal bond strength of base paper, which resulted in smaller delamination that played a role of stress dissipation. It would be recommended to design a proper coating layer in order to prevent fold crack.

• Asian-Australasian Journal of Animal Sciences
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• 제33권6호
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• pp.1023-1033
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• 2020

Objective: The efficiency of the knock-in process is very important to successful gene editing in domestic animals. Recently, it was reported that transient loosening of the nucleosomal folding of transcriptionally inactive chromatin might have the potential to enhance homologous recombination efficiency. The objective of this study was to determine whether histone deacetylases (HDAC) inhibitor and RAD51 recombinase (RAD51) expression were associated with increased knock-in efficiency on the β-casein (bCSN2) gene locus in mammary alveolar-large T antigen (MAC-T) cells using the transcription activator-like effector nucleases (TALEN) system. Methods: MAC-T cells were treated with HDAC inhibitors, valproic acid, trichostatin A, or sodium butyrate for 24 h, then transfected with a knock-in vector, RAD51 expression vector and TALEN to target the bCSN2 gene. After 3 days of transfection, the knock-in efficiency was confirmed by polymerase chain reaction and DNA sequencing of the target site. Results: The level of HDAC 2 protein in MAC-T cells was decreased by treatment with HDAC inhibitors. The knock-in efficiency in MAC-T cells treated with HDAC inhibitors was higher than in cells not treated with inhibitors. However, the length of the homologous arm of the knock-in vector made no difference in the knock-in efficiency. Furthermore, DNA sequencing confirmed that the precision of the knock-in was more efficient in MAC-T cells treated with sodium butyrate. Conclusion: These results indicate that chromatin modification by HDAC inhibition and RAD51 expression enhanced the homologous recombination efficiency on the bCSN2 gene locus in MAC-T cells.

• 복식
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• 제30권
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• pp.295-306
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• 1996

A large quantity of exhumed clothing found for recent 20 years has becom-e the material for making a concrete clarification of the structure of clothing in the Chosun dynasty. Exhumed clothing means the clothing found in the coffin when the grave of the ancestor buried several hundred several hundred years ago is removed to another pla-ce and it is almost comprised of ordinary clothes of the grave-owner not mourning clothes as well as clothing and ornaments in the period between the 15th cen-tury and th 19th century. This research is intended to inquire into the kind of chimas in the 16th century before the Imjin Japancese Invasion of Korea during 1592-1598. The after effect of the Imjin Japanese In-vasion was generally reflected in clothing them and the structure of clothing in the Chosun dynasty began to overshadew its di-chotomy with the Imjin Japanese I vasion of Korea as the point. Accordingly this research is concerned with the study of the chima system of the early Chosun dynasty which shows that there is a common point of the identical form though clothing was exhumed in different graves. Therefore it was possible to grasp women's clothing that was fashionable at that time The object for research is the women in the upper class of the 16th century whose representative characteristics are depicted as follows: 1. Women in the upper class wore the splendid clothing similar to the ordinary clothing of court ladies. 2. The division of ordinary chima and ceremonial chima appeared in the concrete. 3. Ceremonial chima had its extra width and length and exhibited its exaggerated silhouette. in particular it had a various forms of over-pleats made at its center front which play a role in supplementing discomfort in walking. This associate bustle and crinolin styles through which the modern design of clothing can be glimpsed. 4. The opposite phenomenon was shown that has been perceived up to now such as folding at the right plain seam the treatment of the edge-and the like. 5. The elaboration of manual needlework shows the culmination of women's workmanship.

• 복식
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• 제58권8호
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• pp.91-105
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• 2008

Spatial multiplication phenomenon, expressed through de-constructive trend since the late of 20C, have got changed existing fixed and dis-variable space to smooth, fluid, and changeable space. Ex-form and fluidity of new spatial paradigm have been focused through lots of scientific theories studied between digital thought and indeterminacy and rankless nature phenomena. In 21C fashion, indeterminate and irregular form and space have been crested continuously, which could not explain simply according as the fluidity theory Different from the space crested for multi-function and multipurpose, this is revealed the characteristics of chaosmos that communizes the order and the disorder, deconstruction and creation. Ex-formal space of fashion have shown the relationship of de-centerizing, de-territorial, and do-structural phenomenon among different fashion elements. This paper intends to understand the concept of ex-form, and study expressive manners of ex-formal space of 21C fashion, and conclusion as follows. 1) Overlap changes single space of fashion to multi-layered space through the repeat system of pleats, origami, and folding. 2) Ex-gravity expressed in deviation of the gravity acting vertical direction, for example, twisting, curve, winding, portion. 3) Morphing is shown the change process from single fixed form to different complex form. 4) Blurring is expressed in re-combination and re-arrangement among elements of fashion. 5) Blob shows hybrid fashion space through the liberal compounding and separation of a lot of different elements.

• 한국ITS학회 논문지
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• 제6권3호
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• pp.95-100
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• 2007

본 연구에서는 70 kV 임펄스를 사용하는 고출력 시스템에 적합한 두 번 접은 TEM 혼 안테나를 제안하였다. 제시된 구조는 TEM 혼 안테나를 두 번 접음으로서 길이를 50 % 이상 줄일 수 있는 장점을 가진다. 제안된 안테나는 배열된 구조가 되고, 이를 급전하기 위해 체비셰브 다단 변환기를 이용한 스트립 구조의 전력분배기를 사용하였다. 전력분배기는 4개의 TEM 혼 안테나에 동일한 전력과 위상을 공급하며, 광대역($150\;MHz\;{\sim}\;768\;MHz$, VSWR<2.0)으로 동작한다. 제안된 안테나는 70 kV 임펄스에서 정전파괴현상이 발생하지 않도록 금속과 금속과의 간격은 25 mm 이상을 이격하여 설계하였다 실제로 제작 된, 두 번 접힌 TEM 안테나의 크기는 $1730\;{\times}\;1600\;{\times}\;300$ (mm3)이고, 반사손실 10 dB 이하를 기준으로 동작 주파수는 $152\;MHz\;{\sim}\;750\;MHz$, $400\;MHz\;{\sim}\;750\;MHz$에서 안테나 이득은 $6.77\;dBi\;{\sim}\;10.70\;dBi$ 이다.

• 한국의류산업학회지
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• 제17권3호
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• pp.341-350
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• 2015

The purpose of this study was to suggest a silver blouse design by applying modularization. The precedent study of the researcher was used to analyze the design characteristics that were displayed in domestic and foreign silver blouses. This study was taken as the follow-up research as the module components and range of the silver blouse were set up and it was based on to suggest a silver blouse design with application of modularization. The intention was to base on the module system used in various fields to apply the modularization method on the module design research of silver blouse and as a result, it was derived into 6 types. The traditional design reflected on the characteristic of how the silver generation prioritizes practicality and in order to emphasize this, it will be more effective to apply the fastener and folding method for the development of the design. The contemporary design reflected on the characteristic of how the silver generation prioritizes aesthetics and in order to emphasize this, it will be more effective to apply the module composition method where integration can be made according to the desire of the wearer for the development of the design. The trendy design reflected on how the characteristic of the silver generation wants to look younger and the derived modularization method can be applied freely for expression. Since the range, structure, and formation where modularization can be applied are general, it is implied that there is a need to take sustainable development has to be conducted as the tool that can create different types of design.

• 한국펄프종이공학회:학술대회논문집
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• 한국펄프종이공학회 1999년도 Pre-symposium of the 10th ISWPC Recent Advances in Paper Science and Technology
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• pp.386-389
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• 1999

Amphoteric polymers have brought to papermakers' attention gradually. In this paper, a series of amphoteric starches with different cationic and anionic degree of substitution (DS) are prepared. Better product has been selected and used under different condition. As retention and drainage aids, amphoteric starches were used in shorter fiber length, weak strength and poor drainage bleached AQ-Soda wheat straw pulp. In neutral and alkaline papermaking system, when amphoteric starches contain 0.024-0.026 anionic degree of substitution (DS), it is shown that the higher the cationic degree of substitution (DS) in amphoteric starches, the better the filler retention. The filler retention is improved 12.5%, 30.3%, and 35.1% and 32.5% respectively by adding 1% amphoteric starch LS-L2 -1 (0.034), LS-L2-2 (0.040), LS-L2-3 (0.047) and LS-L2-4 (0.052). But the strength of handsheets if affected a little. By adding 2% Al2 (SO4)3 and 1.5% LS-L2-3 at pH 7.5, filler retention can be improved from 38% to 80%, and breaking length of sheet only decreases 3.2%. As dry strengthening aids, amphoteric starches were used in eucalyptus APMP. Amphoteric starches are used in eucalyptus APMP. The amphoteric starch LS-L2-2 is better dry strength aid. Adding 1% LS-L2-2 can improve breaking length 24.5%, burst index 42.9%, tear index 38.8%, folding endurance and density of the handsheets.