• Nuclear Engineering and Technology
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• v.55 no.2
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• pp.655-668
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• 2023

In radiography, an antiscatter grid is a well-known device for eliminating unexpected x-ray scatter. We investigate a new stationary grid artifact suppression method based on a nonsubsampled contourlet transform (NSCT) incorporated with Gaussian band-pass filtering. The proposed method has an advantage that extracts the Moiré components while minimizing the loss of image information and apply the prior information of Moiré component positions in multi-decomposition sub-band images. We implemented the proposed algorithm and performed a simulation and an experiment to demonstrate its viability. We did this experiment using an x-ray tube (M-113T, Varian, focal spot size: 0.1 mm), a flat-panel detector (ROSE-M Sensor, Aspenstate, pixel dimension: 3032 × 3800 pixels, pixel size: 0.076 mm), and carbon graphite-interspaced grids (JPI Healthcare, 18 cm × 24 cm, line density: 103 LP/inch and 150 LP/inch, ratio: 5:1, focal distance: 65 cm). Our results indicate that the proposed method successfully suppressed grid artifacts by reducing them without either reducing the spatial resolution or causing negative side effects. Consequently, we anticipate that the proposed method can improve image acquisition in a stationary grid x-ray system as well as in extended x-ray imaging.

• Journal of the Korean Society for Nondestructive Testing
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• v.22 no.6
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• pp.637-642
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• 2002

An arc-shaped line array slit has been used for the laser generation of focused Lamb waves. The spatially expanded Nd:YAG pulse laser was illuminated through the arc-shaped line array slit on the surface of a sample plate to generate the Lamb waves of the same pattern as the slit. Then the generated Lamb waves were focused at the focal point of which distance from the slit position is dependent on the curvature of slit arc. The proposed method showed better spatial resolution than the conventional linear array slit in the detection of laser machined linear defect and drill machined circular defect on aluminum plates of 2mm thickness. Using the focused waves, we could detect the linear defect and the circular defect with the improvement of spatial resolution. The method can also be combined with the scanning mechanism to get an image just like by the scanning acoustic microscope(SAM).

• Journal of Microbiology and Biotechnology
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• v.22 no.12
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• pp.1629-1635
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• 2012

Previously, we demonstrated that the erythropoietin receptor (EpoR) is present on fibroblasts, where it regulates focal contact. Here, we assessed whether this action of EpoR is involved in the reduced cell adhesion observed in colonocytes exposed to Clostridium difficile toxin A. EpoR was present and functionally active in cells of the human colonic epithelial cell line HT29 and epithelial cells of human colon tissues. Toxin A significantly decreased activating phosphorylations of EpoR and its downstream signaling molecules JAK-2 (Janus kinase 2) and STAT5 (signal transducer and activator of transcription 5). In vitro kinase assays confirmed that toxin A inhibited JAK 2 kinase activity. Pharmacological inhibition of JAK2 (with AG490) abrogated activating phosphorylations of EpoR and also decreased focal contacts in association with inactivation of paxillin, an essential focal adhesion molecule. In addition, AG490 treatment significantly decreased expression of occludin (a tight junction molecule) and tight junction levels. Taken together, these data suggest that inhibition of JAK2 by toxin A in colonocytes causes inactivation of EpoR, thereby enhancing the inhibition of focal contact formation and loss of tight junctions known to be associated with the enzymatic activity of toxin A.

• International Journal of Oral Biology
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• v.37 no.4
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• pp.189-195
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• 2012

Resistance to the induction of apoptosis is a possible mechanism by which tumor cells can survive anti-neoplastic treatments. Melanoma is notoriously resistant to anti-neoplastic therapy. Previous studies have demonstrated focal adhesion kinase (FAK) overexpression in melanoma cell lines. Given its probable role in mediating resistance to apoptosis, many researchers have sought to determine whether the downregulation of FAK in melanoma cells would confer a greater sensitivity to anti-neoplastic agents. Genistein is a known inhibitor of protein-tyrosine kinase (PTK), which may attenuate the growth of cancer cells by inhibiting the PTK-mediated signaling pathway. This present study was undertaken to investigate the effect of genistein on the expression of FAK and cell cycle related proteins in the G361 melanoma cell line. Genistein was found to have a preferential cytotoxic effect on G361 melanoma cells over HaCaT normal keratinocytes. Genistein decreased the expression of 125 kDa phosphotyrosine kinase and the FAK protein in particular. Genistein treatment did not affect the expression of p53 in G361 cells in which p21 is upregulated. The expression of cyclin B and cdc2 was downregulated by genistein treatment. Taken together, our data indicate that genistein induces the decreased proliferation of G361 melanoma cells via the inhibition of FAK expression and regulation of cell cycle genes. This suggests that the use of genistein may be a viable approach to future melanoma treatments.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.22 no.6
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• pp.1259-1265
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• 1997

We investigate depth measurement error of a range finder based on triangulation method. Geometric analysis resulted in intuitive understanding of the error sensitivity. We show that the depth error is propostional to the distance between the object andthe camera. The measurement value has the highest accuracy when the line connecting the focal point of the camera and the object is perpendicular to the line joining the object and the light source of herange finder. Also we analyze the error using a perturbation method and verify that the results are identical through an experiment.

• 제어로봇시스템학회:학술대회논문집
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• 2002.10a
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• pp.100.5-100
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• 2002

A core technology for implementation of Augmented Reality is to develop a merging algorithm between interesting 3-D objects and real images. In this paper, we present a 3-D object recognition method to decide viewing direction toward the object from camera. This process is the starting point to merge with real image and 3-D objects. Perspective projection between a camera and 3-dimentional objects defines a plane in 3-D space that is from a line in an image and the focal point of the camera. If no errors with perfect 3-D models were introduced in during image feature extraction, then model lines in 3-D space projecting onto this line in the image would exactly lie in this plane. This observa...

• Journal of the Optical Society of Korea
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• v.18 no.5
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• pp.611-615
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• 2014

We report high-speed pulse train generation from a relatively low-speed 10-GHz mode-locked laser by means of line-by-line spectral coding. To increase the pulse repetition rate multiplication (RRM) factor, we combine coding schemes for both spectral intensity and phase by placing a simple mask at the coder focal plane. The resulting RRM factor, determined by multiplying the RRM factors of the individual coding schemes, rises as high as 16. To verify the generated pulses, the optical spectra and autocorrelation traces are examined.

• The Transactions of the Korean Institute of Electrical Engineers D
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• v.50 no.4
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• pp.161-167
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• 2001

Camera calibration is very important problem in 3D measurement using vision system. In this paper is proposed the simple method for camera calibration. It is designed that uses the principle of vanishing points and the concept of corresponding points extracted from the parallel line pairs. Conventional methods are necessary for 4 reference points in one frame. But we proposed has need for only 2 reference points to estimate vanishing points. It has to calculate camera parameters, focal length, camera attitude and position. Our experiment shows the validity and the usability from the result that absolute error of attitude and position is in $10^{-2}$.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.24
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• pp.10797-10801
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• 2015

Background: To explore the molecular mechanisms of metastatic osteosarcoma (OS) by using the microarray expression profiles of metastatic and non-metastatic OS samples. Materials and Methods: The gene expression profile GSE37552 was downloaded from Gene Expression Omnibus database, including 2 human metastatic OS cell line models and 2 two non-metastatic OS cell line models. The differentially expressed genes (DEGs) were identified by Multtest package in R language. In addition, functional enrichment analysis of the DEGs was performed by WebGestalt, and the protein-protein interaction (PPI) networks were constructed by Hitpredict, then the signal pathways of the genes involved in the networks were performed by Kyoto Encyclopaedia of Genes and Genomes (KEGG) automatic annotation server (KAAS). Results: A total of 237 genes were classified as DEGs in metastatic OS. The most significant up- and down-regulated genes were A2M (alpha-2-macroglobulin) and BCAN (brevican). The DEGs were significantly related to the response to hormone stimulus, and the PPI network of A2M contained IL1B (interleukin), LRP1 (low-density lipoprotein receptor-related protein 1) and PDGF (platelet-derived growth factor). Furthermore, the MAPK signaling pathway and focal adhesion were significantly enriched. Conclusions: A2M and its interactive proteins, such as IL1B, LRP1 and PDGF may be candidate target molecules to monitor, diagnose and treat metastatic OS. The response to hormone stimulus, MAPK signaling pathway and focal adhesion may play important roles in metastatic OS.

• Journal of the Korean Society for Precision Engineering
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• v.27 no.3
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• pp.18-26
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• 2010

Diffraction gratings with precise spatial periods of 2 ${\mu}m$ and 5 ${\mu}m$ have been fabricated by using a femtosecond laser which does not have limits on materials of micromachining and small thermal effects due to high peak power. Diffraction angle and diffraction efficiency of those were measured. Simulation results of diffraction angle and diffraction efficiency of the diffraction grating calculated with the parameters such as line width, depth, and spatial period of the fabricated gratings were compared with experimental results measured with a He-Ne laser. Besides these, Fresnel Zone Plates (FZPs) with focal distances of 50 mm and 25 mm were fabricated and focal distances of fabricated FZP were measured. Those experimental results for diffraction gratings and FZPs match well with experimental results.