• Journal of Bio-Environment Control
• /
• v.33 no.2
• /
• pp.88-98
• /
• 2024

This study provides basic data on the growth and production of seedlings produced in plant factories with artificial lighting by comparing seedling quality, growth and fruit characteristics, and production after transplanting cucumber seedlings according to environmental differences between plant factories with artificial lighting and conventional nurseries in greenhouse. The control group consisted of greenhouse seedlings (GH) grown in the conventional nursery before transplanting. Plant factory to greenhouse seedlings (PG) were grown for 9 days in a plant factory with artificial lighting and for 13 days in an conventional nursery. Plant factory seedlings (PF) were grown in a plant factory with artificial lighting for 22 days until planting. In terms of seedling quality, PFs had the highest relative growth rate and compactness and the best root zone development. After transplanting PFs tended to grow faster, the first harvest date was 2 days earlier than that of GHs, and the growing season ended 1 day earlier. The female flower flowering rate of the PFs was high, and the fruit set rate was of PF the lowest. The production per unit area was highest for PFs at 10.23kg Performance index on the absorption basis, the most sensitive chlorophyll fluorescence parameter, was highest at 4.14 for PFs at 4 weeks after transplantation. By comparing the maximum quantum yield of primary PS II photochemistry and dissipated energy flux per PS II reaction center electron at 4 weeks after transplantation, PFs tended to be the least stressed. PFs had the best seedling quality, growth, and production after planting, and fruit quality was consistent with that of greenhouse seedlings. Therefore, plant factory seedlings can be used in the field.

• Clinical and Experimental Reproductive Medicine
• /
• v.36 no.1
• /
• pp.45-53
• /
• 2009

Objectives: To determinate the optimal culture condition to maintain lifespan in human sperm, we evaluated the effect of different temperature on sperm motility and viability up to 5 days in normal specimens. Methods: Ejaculated semen samples with normal semen parameters were gently washed in HEPES buffered Tyrod's-Albumin-Lactate-Pyruvate (HTALP) media. Each 5 ml of HTALP + 0.3% albumin with $1{\times}10^6$ sperm/ml was incubated for 5 days in $37^{\circ}C$, $22^{\circ}C$, and $4^{\circ}C$. The sperm motility and kinematics were analyzed using computer assisted sperm analysis (CASA), membrane integrity was assessed by hypoosmotic swelling test (HOST), and capacitation status was evaluated by chlorotetracycline (CTC) fluorescence pattern. Each parameter was measured on day 1, 3, and 5, respectively. Results: The motility, viability and live/uncapacitated pattern were demonstrated significantly in temperature- and time-dependent difference (p<0.05). While the sperm cultured for 1 day in each temperature was not significantly different, the sperm cell kept in $22^{\circ}C$ after 3 days were preserved sperm motility, viability, membrane integrity, and F pattern better than in other culture temperatures. Conclusions: HTALP can be used a basic medium for culture and longevity preservation, and sperm cell kept at $22^{\circ}C$ is beneficial for assisted reproductive techniques.

• Journal of Plant Biology
• /
• v.38 no.4
• /
• pp.373-380
• /
• 1995

Photoinhibition of photosystem (PS) n was induced in primary leaves of 25 day-old peppers grown $100\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1},\;at\;25^{\circ}C$. The modulation of PSII functionality in vivo was induced by varying both irradiance ($0-3000\;{\mu}molm^{-2}{\cdot}s^{-1}$) and duration (0-70 min) of light treatment. The functionality of PSII was investigated in terms of photochemical efficiency of PSII (Fv/Fm) and quantum yield of $O_2$ evolution, and expressed as a function of photon exposure [$mol\;photons{\cdot}m^{-2}$, the product of irradiance and duration of light treatment (Bell and Rose, 1981)]. Contrary to the linear decline of Fv/Fm ratio showing 50% decreases by absorption of $10\;mol\;photons{\cdot}m^{-2$, quantum yield of $O_2$ evolution decreased biphasically with increasing photon exposure, showing 50% decreases by absorption of $5.5\;mol\;photons{\cdot}m^{-2}$. Treatment of low temperature at $15^{\circ}C$ for 30 min alone did not affect the functionality of PSII, but high temperature ($45^{\circ}C$) significantly inactivated PSII activity. However, when Jeaves of pepper were subjected to low or high temperature in the presence of light, PSII was substantially photoinactivated. These results suggest the presence of different photoinhibitory mechanisms at low and high temperature.rature.

• Korean Journal of Clinical Laboratory Science
• /
• v.50 no.4
• /
• pp.399-405
• /
• 2018

The platelet count in clinical laboratories is essential for the diagnosis and treatment of hemostasis abnormalities, and accurate platelet counting in the low count range is of prime importance for deciding if a platelet transfusion is needed and for monitoring after chemotherapy. Quality control is designed to reduce and correct any deficiencies in the internal analytical process of a clinical laboratory prior to the release of patient results. Fragmented erythrocytes are the major confusing factors for platelet counting because of their similar size to platelets. The authors found that the low range QC values were out of 2SD with a Sysmex automatic analyzer in internal quality control process. Thus far, there has been little discussion on the relationship between hemolysis and the platelet parameters. Therefore, this study focused on the performance of automated platelet counts, including the PLT-F, the PLT-I, and PLT-O methods at the low platelet range using the low level QC materials and compared the 5 platelet parameters with the hemolyzed samples. The results showed that the CV was the smallest with PLT-F and P-LCR increased from 18.4 to 31.9% in the hemolysis samples. These results indicate that a more accurate estimation of the platelet counts can be achieved using the PLT-F method than the PLT-I method at the low platelet range. The use of the PLT-F system improves the confidence of results in low platelets samples in a routine hematology laboratory. The results suggest that P-LCR is a new parameter in assessing samples when the specimen is suspected of hemolysis and deterioration. Nevertheless, further studies will be needed to establish the relationship with P-LCR and hemolysis using human blood specimens.