• 한국작물학회지
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• 제60권1호
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• pp.1-7
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• 2015

본 연구는 파종시기와 질소시비 수준에 대한 옥수수의 광화학적 반응을 분석하여 광생리적 특성을 해석하였다. 1. 5월 22일 파종시 질소 함량이 증가함에 따라 생육후기까지 엽록소 형광량 또한 증가하여 질소 배량처리에서 광이용효율이 더 높은 것으로 확인되었다. 2. 생육초기의 형광량이 가장 높았으며, 이후 모든 처리구에서 엽록소 형광량이 50% 가량 감소하여 전자전달을 위한 광화학 반응이 크게 감소했음을 알 수 있었다. 3. 질소 반량구에서 활성화된 RC (RC/ABS)가 감소하며 13% 이상의 에너지가 손실되었으며, 결국 전자전달시 에너지 전환효율(PI, DF 등)이 감소해 광이용효율이 낮았다. 4. 배량 처리구는 생육후기로 갈수록 전자전달 효율(ET2o/CS) 및 단면당 활성화된 RC(RC/CS) 등 광이용 효율이 대조구 대비 크게 증가하여 광합성기구 사이의 전자전달이 잘 이루어지는 것으로 확인되었다.

• Food Science and Biotechnology
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• 제15권4호
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• pp.534-542
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• 2006

The effects of ethanol fractions of three different rice grain extracts, Jakwangchalbyeo, Hwasunchalbyeo, and Ilpumbyeo, on apoptotic cell death in the rat hepatoma H4IIE cell line were investigated using the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] cell viability assay. One hundred mg/mL Jakwangchalbyeo extract significantly reduced cell viability to 69.5, 57.2, and 46.1% within 24, 48, and 72 hr, respectively. Fluorescence-activated cell sorting (FACS) analyses were also performed to characterize the cell death pattern caused by treatment with the rice grain extracts. Apoptotic cell death was clearly observed with time after treatment with the Jakwangchalbyeo extract. In Western blotting analysis, degradation of the 116 kDa poly-ADP-ribose polymerase (PARP) molecule was observed with concomitant formation of an 89 kDa product 24, 48, and 72 hr after treating cells with the Jakwangchalbyeo extract. This indicates that an apoptotic process caused cell death in these cells. In conclusion, red-pericarp Jakwangchalbyeo extract induced apoptotic cell death in H4IIE cells to a larger extent than the other rice extracts.

• Tuberculosis and Respiratory Diseases
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• 제77권3호
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• pp.116-123
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• 2014

Background: Mesenchymal stem cells (MSCs) obtained from bone marrow or adipose tissue can successfully repair emphysematous animal lungs, which is a characteristic of chronic obstructive pulmonary disease. Here, we describe the cellular distribution of MSCs that were intravenously injected into mice with elastase-induced emphysema. The distributions were also compared to the distributions in control mice without emphysema. Methods: We used fluorescence optical imaging with quantum dots (QDs) to track intravenously injected MSCs. In addition, we used a human Alu sequence-based real-time polymerase chain reaction method to assess the lungs, liver, kidney, and spleen in mice with elastase-induced emphysema and control mice at 1, 4, 24, 72, and 168 hours after MSCs injection. Results: The injected MSCs were detected with QD fluorescence at 1- and 4-hour postinjection, and the human Alu sequence was detected at 1-, 4- and 24-hour postinjection in control mice (lungs only). Injected MSCs remained more in mice with elastase-induced emphysema at 1, 4, and 24 hours after MSCs injection than the control lungs without emphysema. Conclusion: In conclusion, our results show that injected MSCs were observed at 1 and 4 hours post injection and more MSCs remain in lungs with emphysema.

• 펄프종이기술
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• 제48권3호
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• pp.5-13
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• 2016

This study investigated the effects of different factors on the migration of a fluorescent whitening agent (FWA) from paper treated with FWAs to non-fluorescent papers. FWA migration experiments were carried out in vertical and friction contacts between the papers dyed with FWAs and non-fluorescent papers. During the experiments, we identified the effects of the addition and types of FWAs, contact time, temperature, and relative humidity (RH) on FWA migration. The fluorescence indices of the non-fluorescent papers were measured before and after the migration experiments, and the Student's t test, a statistical tool, was utilized to compare results from different migration experiments. In vertical contact experiments, FWA migration to non-fluorescent paper was observed at $30^{\circ}C$ and 70% RH; this was attributed to the high moisture content of the paper. FWA migration did not occur significantly at $23^{\circ}C$ and 50% RH. In the friction contact experiments, FWA migrations were identified at both temperature conditions and RH percentages. The addition and types of FWAs did not increase the fluorescence index of non-fluorescent papers. Therefore, it was concluded that the moisture content of paper and the friction contact affected FWA migration from the papers containing internal and surface FWAs.

• 한국콘크리트학회:학술대회논문집
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• 한국콘크리트학회 2003년도 가을 학술발표회 논문집
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• pp.198-201
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• 2003

The R.C Building will be superannuated as time passes. This program is generated by propagation of cracks. In order to manage such cracks, time and efforts, expense, etc. are required. In this study, glass sensors were embedding in a model beam and column and leakage of fluorescence and adhesive material was investigated. Further, currents in glass pipe were observed to find the leakage of liquid in glass pipes. Progressive cracks generated by cause the fracture of glass pipes. Therefore, the liquid become to flow and electric current stops, and the cracked part of the member can be found easily. Moreover, the adhesive delays progressive cracking system that responds in air, and the life of a structure can be made to extend. The purpose of this research is to develop of low price sensors that can perform of self-diagnosis in addition to ability of concrete repair concrete to damage.

• Corrosion Science and Technology
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• 제4권1호
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• pp.29-32
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• 2005

A various structural materials are used in construction projects such as a stone, concrete, steel materials. Between of them, concrete are used widely. The compressive strength of concrete is high, and its maintenance and management is comparatively easy. The R.C Building will be superannuated as time passes. This program is generated by propagation of cracks. In order to manage such cracks, time and efforts, expense, etc. are required. In this study, glass sensors were embedding in a model beam and column and leakage of fluorescence and adhesive material was investigated. Further, currents in glass pipe were observed to find the leakage of liquid in glass pipes. Progressive cracks generated by cause the fracture of glass pipes. Therefore, the liquid become to flow and electric current stops, and the cracked part of the member can be found easily. Moreover, the adhesive delays progressive cracking system that responds in air, and the life of a structure can be made to extend. The purpose of this research is to develop of low price sensors that can perform of self-diagnosis in addition to ability of concrete repair concrete to damage.

• Molecules and Cells
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• 제38권1호
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• pp.81-88
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• 2015

Flowering time (or heading date) is controlled by intrinsic genetic programs in response to environmental cues, such as photoperiod and temperature. Rice, a facultative short-day (SD) plant, flowers early in SD and late in long-day (LD) conditions. Casein kinases (CKs) generally act as positive regulators in many signaling pathways in plants. In rice, Heading date 6 (Hd6) and Hd16 encode $CK2{\alpha}$ and CKI, respectively, and mainly function to delay flowering time. Additionally, the major LD-dependent floral repressors Hd2/Oryza sativa Pseudo-Response Regulator 37 (OsPRR37;hereafter PRR37) and Ghd7 also confer strong photoperiod sensitivity. In floral induction, Hd16 acts upstream of Ghd7 and CKI interacts with and phosphorylates Ghd7. In addition, Hd6 and Hd16 also act upstream of Hd2. However, whether CKI and $CK2{\alpha}$ directly regulate the function of PRR37 remains unclear. Here, we use in vitro pull-down and in vivo bimolecular fluorescence complementation assays to show that CKI and $CK2{\alpha}$ interact with PRR37. We further use in vitro kinase assays to show that CKI and $CK2{\alpha}$ phosphorylate different regions of PRR37. Our results indicate that direct posttranslational modification of PRR37 mediates the genetic interactions between these two protein kinases and PRR37. The significance of CK-mediated phosphorylation for PRR37 and Ghd7 function is discussed.

• Interdisciplinary Bio Central
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• 제4권2호
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• pp.5.1-5.9
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• 2012

Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Western-blotting of proteins of interest especially when specific antibodies for the proteins are not available. However, the conventional Western-blotting requires the multi-steps process taking at least several hours up to two days. With examples of various applications, here we show a convenient and time-saving method for protein detection which employs a fluorescent chemical BDED and its binding peptide RC-tag. And we propose "Estern staining", as a standard term for protein detection method using fluorescent chemicals and their binding small peptide tags. Eastern staining may substitutes for the time-consuming "immuno-staining" in many versatile applications.

• KSBB Journal
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• 제22권4호
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• pp.260-264
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• 2007

본 실험에서는 마이크로어레이 형태로 펩타이드와의 공유결합에 의한 고정화를 시키기 위해 유리 칩의 표면을 아민기에서 thiol기로 개질하였다. 펩타이드의 lysine기와 thiol기와의 공유결합반응에는 12시간 정도의 반응시간이 필요하였고 실온보다는 35$^{\circ}C$가 유리함을 확인하였다. Trypsin-FITC와의 반응을 통해 trypsin 결합부위를 가진 target 펩타이드가 control 펩타이드보다 더 높은 형광 신호를 나타냄을 확인하였고, 이를 통해 target 펩타이드를 마이크로어레이 상에서 식별할 수 있었다. 이 trypsin-FITC와의 결합 친화도 차이를 별도의 QCM 실험을 통해서도 확인하였다. 또한 작은 부피의 spot과 높은 농도의 펩타이드 용액이 더욱 높은 표면형광신호를 생성함을 확인하였다. 본 실험을 통해 펩타이드 마이크로어레이 칩 개발을 위한 기초 조건을 확립하였다.

• Environmental Engineering Research
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• 제17권4호
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• pp.205-210
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• 2012

Hydroperoxyl radical/superoxide anion radical ($HO_2{\cdot}/O_2^-{\cdot}$, $pK_a$=4.8) as an intermediate is of considerable importance in oxidation processes. Hence, the method of detecting $HO_2{\cdot}/O_2^-{\cdot}$ with high sensitivity is necessary to be developed. To achieve this objective, this study newly employed terephthalate (TA) as a probe for the measurement of $HO_2{\cdot}/O_2^-{\cdot}$ in the kinetic method presented in our previous study. This method was based on the hydroxylation of TA to produce mainly hydroxyterephthalic acid or hydroxyterephthalate (OHTA), which was analyzed by fluorescence detection (${\lambda}_{ex}$=315nm, ${\lambda}_{ex}$=425nm). The life-time of $HO_2{\cdot}/O_2^-{\cdot}$ and its concentration formed from the photolysis technique of $H_2O_2$ were reported in this study. At range of pH 2-10, the life-time of $HO_2{\cdot}/O_2^-{\cdot}$ was 51-422 sec. In particular, an increase in the life-time with pH was observed. The sensitivities of the kinetic method by using TA were always higher with 1.7-2.5 times at pH 8.0 than those by using benzoic acid. From these results, this study can contribute to understanding the basic functions of $HO_2{\cdot}/O_2^-{\cdot}$ in oxidation processes.