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http://dx.doi.org/10.4051/ibc.2012.4.2.0005

Eastern Staining: A Simple Recombinant Protein Detection Technology Using a Small Peptide Tag and Its Counter Partner Which is a Fluorescent Compound  

Lee, Jae-Jung (Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research)
Kim, Jun-Young (Department of Chemistry, MedChem Program of Life Sciences Institute, National University of Singapore)
Zhai, Duanting (Department of Chemistry, MedChem Program of Life Sciences Institute, National University of Singapore)
Yun, Seong-Wook (Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research)
Chang, Young-Tae (Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research)
Publication Information
Interdisciplinary Bio Central / v.4, no.2, 2012 , pp. 5.1-5.9 More about this Journal
Abstract
Small peptide tags such as c-myc, HA, or FLAG tag have facilitated efficient Western-blotting of proteins of interest especially when specific antibodies for the proteins are not available. However, the conventional Western-blotting requires the multi-steps process taking at least several hours up to two days. With examples of various applications, here we show a convenient and time-saving method for protein detection which employs a fluorescent chemical BDED and its binding peptide RC-tag. And we propose "Estern staining", as a standard term for protein detection method using fluorescent chemicals and their binding small peptide tags. Eastern staining may substitutes for the time-consuming "immuno-staining" in many versatile applications.
Keywords
Western blot; Estern staining; fluorescence dye BDED; RC-tag; peptide chemical tags;
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