Eastern Staining: A Simple Recombinant Protein Detection Technology Using a Small Peptide Tag and Its Counter Partner Which is a Fluorescent Compound |
Lee, Jae-Jung
(Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research)
Kim, Jun-Young (Department of Chemistry, MedChem Program of Life Sciences Institute, National University of Singapore) Zhai, Duanting (Department of Chemistry, MedChem Program of Life Sciences Institute, National University of Singapore) Yun, Seong-Wook (Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research) Chang, Young-Tae (Laboratory of Bioimaging Probe Development, Singapore Bioimaging Consortium, Agency for Science, Technology and Research) |
1 | Brizzard, B. (2008). Epitope tagging. BioTechniques 44, 693-695. DOI |
2 | Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol 60, 523-533. DOI |
3 | Evan, G.I., Lewis, G.K., Ramsay, G., and Bishop, J.M. (1985). Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product. Mol Cell Biol 5, 3610-3616. DOI |
4 | Einhauer, A., and Jungbauer, A. (2001). The FLAG peptide, a versatile fusion tag for the purification of recombinant proteins. J Biochem Biophys Meth 49, 455-465. DOI |
5 | Prescher, J.A., and Bertozzi, C.R. (2005). Chemistry in living systems. Nat Chem Biol 1, 13-21. DOI |
6 | Griffin, B.A., Adams, S.R., and Tsien, R.Y. (1998). Specific covalent labeling of recombinant protein molecules inside live cells. Science 281, 269-272. DOI |
7 | Girouard, S., Houle, M.H., Grandbois, A., Keillor, J.W., and Michnick, S.W. (2005). Synthesis and characterization of dimaleimide fluorogens designed for specific labeling of proteins. J Am Chem Soc 127, 559-566. DOI |
8 | Lee, J.J., Lee, S.C., Zhai, D., Ahn, Y.H., Yeo, H.Y., Tan, Y.L., and Chang, Y.T. (2011). Bodipy-diacrylate imaging probes for targeted proteins inside live cells. Chem Commun (Camb) 47, 4508-4510. DOI |
9 | Einarson, M.B. (2001). In Molecular Cloning: A Laboratory Manual, 3rd Edition (Cold Spring Harbor Kaboratory Press). |
10 | Hengen, P. (1995). Purification of His-Tag fusion proteins from Escherichia coli. Trends Biochem Sci 20, 285-286. DOI |
11 | Donovan, R.S., Robinson, C.W., and Glick, B.R. (1996). Review: optimizing inducer and culture conditions for expression of foreign proteins under the control of the lac promoter. J Ind Microbiol 16, 145-154. DOI |
12 | Krishnan, B., Szymanska, A., and Gierasch, L.M. (2007). Site-specific fluorescent labeling of poly-histidine sequences using a metal-chelating cysteine. Chem Biol Drug Des 69, 31-40. DOI |
13 | Chen, I., Howarth, M., Lin, W., and Ting, A.Y. (2005). Site-specific labeling of cell surface proteins with biophysical probes using biotin ligase. Nat Methods 2, 99-104. DOI |
14 | Zhao, C., Hellman, L.M., Zhan, X., Bowman, W.S., Whiteheart, S.W., and Fried, M.G. (2010). Hexahistidine-tag-specific optical probes for analyses of proteins and their interactions. Anal Biochem 399, 237-245. DOI |
15 | Los, G.V., Encell, L.P., McDougall, M.G., Hartzell, D.D., Karassina, N., Zimprich, C., Wood, M.G., Learish, R., Ohana, R.F., Urh, M., et al. (2008). HaloTag: a novel protein labeling technology for cell imaging and protein analysis. ACS Chem Biol 3, 373-382. DOI |
16 | Keppler, A., Gendreizig, S., Gronemeyer, T., Pick, H., Vogel, H., and Johnsson, K. (2003). A general method for the covalent labeling of fusion proteins with small molecules in vivo. Nat Biotechnol 21, 86-89. DOI |
17 | Fernandez-Suarez, M., Baruah, H., Martinez-Hernandez, L., Xie, K.T., Baskin, J.M., Bertozzi, C.R., and Ting, A.Y. (2007). Redirecting lipoic acid ligase for cell surface protein labeling with small-molecule probes. Nat Biotechnol 25, 1483-1487. DOI |