• The Korean Journal of Community Living Science
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• v.15 no.1
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• pp.67-76
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• 2004

In order to promote the value of the flowers as new agricultural products, we investigated the biological activities of rape, arrowroot, and rose extracts. Biological activities investigated included antioxidant activity and the effects on 3T3-L1 fibroblast cells. When each flower was extracted with methanol, the antioxidant index and electron donating activity of roses was the highest $(IC_{50}$ of rose extract was $17.6 \mu{g}/m\ell$). When 3T3-L1 fibroblast cells were treated with extracts made with hexane, ethyl acetate, and ether, the rape extracts had a cytotoxic effect on the cells. 12.2% of cells survived when treated with a 3mg/$m\ell$ ether extract while those treated with the same concentration of hexane and ethyl acetate had survival rates of 76.2% and 78.6% respectively. In contrast to rape, the ether extract of arrowroot and rose stimulated the growth of 3T3-L1 cells. The effect of rose extracts was much bigger than those of other extracts. Although every rose extract stimulated the growth of the 3T3-L 1 cells, the ether extract stimulated growth up to 168.6% compared to the control at the concentration of $0.3mg/m\ell$, and 148.3% at the concentration of $1mg/m\ell$. The toxicity on cells treated with $H_2 O_2$ of $450\mu{M}l$was decreased with the addition of rose extract. The survival rate after treatment with rose extract at the concentration of $100\mu{g}/m\ell$ was increased to 71% compared to the 32% survival rate of control. From these results, it can be concluded that the extracts of arrowroot and rose seem to stimulate cells, whereas the extract of rape has a cytotoxic effect. Biological activities of ether extract were the strongest compared to those of other extracts at the tested concentrations.

• Korean Journal of Medicinal Crop Science
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• v.19 no.4
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• pp.271-275
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• 2011

In this study, ginseng flower water extracts were analyzed to set up the ginsenoside content and quality optimization condition. The highest total ginsenoside content among the ginseng flower water extracts was 67.44mg/g which was extracted at $85^{\circ}C$ for 3 hours. In addition, the ginsenoside content decreased according to the increased extraction temperature and time. The highest total content of $Rb_2$ and Re was 37.42mg/g at $75^{\circ}C$ for 6 hours. Total content of $Rb_2$ and Re decreased according to the increased extraction temperature and time. The highest prosapogenin ($Rg_2$ + $Rg_3$ + $Rh_1$) content among the total of ginseng flower water extracts was 18.58mg/g which was extracted at $95^{\circ}C$ for 12 hours. The sweetness, absorbance were increased according to the increased extraction temperature and time. But pH was decreased according to the increased extraction time.

• Food Science and Preservation
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• v.18 no.5
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• pp.739-745
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• 2011

The major objective of this study was to investigate the antioxidant activities of methanolic extracts from different parts (flower, leaf stem, and root) of Chrysanthemum zawadskii by employing various in-vitro assay systems. The extraction yields from the flower, leaf stem, and root were 18.347, 12.93, and 11.33-----, respectively. The total polyphenol content was highest in the flower (17.16 mg/100 g) and lowest in the root (11.33 mg/100 g). The antioxidant activities were raised within creasing amounts of extracts, and the extracts from the flower showed the highest effect on the superoxide anion radical scavenging, metal chelating on ferrous ions and reducing power. In addition, the leaf stem also showed good antioxidant activity in various systems. These results suggest that the methanolic extracts from the flower and leaf stem possess excellent antioxidant activities and may thus serve as potential sources of natural antioxidants.

• Korean Journal of Medicinal Crop Science
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• v.23 no.3
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• pp.190-197
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• 2015

In this study, we tried to offer the possibility of White Hibiscus syriacus L. (WHS) flower extracts as a preventive and improving agent of osteoporosis that bone mass reduction is induced by an decrease of osteoblast involved in bone formation and increase of bone resorption by osteoclast activity. As a results, it was found to have antioxidant activity and contain a flavonoid contents ($47.74 mg/g) of the WHS flower. There was cytotoxicity at more than $250{\mu}g/m{\ell}$ concentration of WHS flower extract of RANKL-induced osteoclast in RAW264.7. There were no significant inhibited TRAP activity in the WHS leaf and stem. However, it was confirmed that it is significantly inhibited the differentiation activity of osteoclasts in 50 and $100{\mu}g/m{\ell}$ concentration of cells of stability levels of only WHS flower extracts (p < 0.01). The WHS flower prominently inhibited RANKL-induced osteoclast differentiation activity by decreased calcitonin receptor and TRAP mRNA (p < 0.01). These results indicate that of osteoclasts differentiation activity is inhibited by protection of oxidative stress due to the antioxidant activity of the WHS flower. Therefore, suggesting the WHS flower may be a presents the possibility as a preventive and therapeutic agents for osteoporosis.

• Current Research on Agriculture and Life Sciences
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• v.33 no.2
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• pp.49-56
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• 2015

The aim of this research was to investigate the anti-oxidantive activities of extracts from Chionanthus retusus leaves, fruits and flower. The content of phenolics were 20.8 mg/g in water extracts and 32.2 mg/g in 90% ethanol extracts from flower. The DPPH free radical scavenging activity of extracts from Chionanthus retusus was above 70% at phenolic concentration $100{\mu}g/mL$. The ABTS radical decolorization activities of water and ethanol extracts were both above 80% at $100{\mu}g/mL$ phenolic concentration, respectively. The antioxidant protection factor(PF) of water and 80% ethanol extracts from leaves was the highest as 2.27 PF and 1.70 PF at $50{\mu}g/mL$ phenolic concentration. The TBARs inhibition rate of the Chionanthus retusus extracts, was above 70% at $200{\mu}g/mL$ phenolic concentration. The anti-oxidantive activities of extracts from leaves were shown more active than BHT as a positive control except TBARs. These results confirmed that the extracts from Chionanthus retusus leaves, fruits and flower was shown the high anti-oxidant activity. The results can be expected isolated phenolic compounds from Chionanthus retusus to use as functional beauty food resource.

• Proceedings of the Plant Resources Society of Korea Conference
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• v.18 no.1
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• pp.41-51
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• 2005

We examined whether extracts from 14 local citrus spp. on Jeju Island (Korea) contained chemosensitizing activity that would increase the cytotoxic effect of vincristine(VCR) in drug-resistant cancer cells. We report that methanol extracts from fruits and flowers of some species had a chemosensitizing effect that reversed P-glycoprotein (Pgp)-mediated multidrug resistance (MDR). Using drug-sensitive AML-2/WT and drug-resistant AML-2/D100 in the absence of VCR in human acute myelogenous leukemia cells we found that fruit or flower extracts alone generally had low cytotoxicity $(IC_{50}>200\;{\mu}g/ml)$. In studies examining the effect of extracts on 120 ng/ml VCR cytotoxicity in drug-resistant AML-2/D100 cells, we found that immature fruit extracts had greater chemosensitizing activity than either extracts from mature fruit or flower. Of the 14 species examined, the immature fruit extract from Inchangkyool (Citrus ichangiensis) showed the hishest chemosensitizing index(CI) valus. Immature fruit extracts of Hongkyool(C. tachibana), Byungkyool(C. platymamma), Cheongkyool(C. nippokoreana) and Jinkyool (C. sunki) also strongly potentiated VCR cytotoxicity in AML-2/D100 cells. The chemosensitizing effect of peel extracts was 2-10-fold that of whole fruit extracts from Hongkyool (C. tachibana), Byungkyool (C. platymamma) and Inchangkyool (C. inchangiensis). The CI values for flower extracts were higher than those for mature fruit extracts, but lower than those for immature fruit extracts. These results indicate that immature citrus fruits contain compounds that do not exert their activity solely through cytotoxicity. In particular, Incahngkyool (C. inchangiensis), Byungkyool(C.platymamma), Cheongkyool(C. nippokoreana) and Hongkyool (C. tahibana) may be useful sources of chemosensitizing compounds.

• Parasites, Hosts and Diseases
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• v.54 no.3
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• pp.273-280
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• 2016

We evaluated the activity of methanolic extracts of Melaleuca cajuputi flowers against the filarial worm Brugia pahangi and its bacterial endosymbiont Wolbachia. Anti-Wolbachia activity was measured in worms and in Aedes albopictus Aa23 cells by PCR, electron microscopy, and other biological assays. In particular, microfilarial release, worm motility, and viability were determined. M. cajuputi flower extracts were found to significantly reduce Wolbachia endosymbionts in Aa23 cells, Wolbachia surface protein, and microfilarial release, as well as the viability and motility of adult worms. Anti-Wolbachia activity was further confirmed by observation of degraded and phagocytized Wolbachia in worms treated with the flower extracts. The data provided in vitro and in vivo evidence that M. cajuputi flower extracts inhibit Wolbachia, an activity that may be exploited as an alternative strategy to treat human lymphatic filariasis.

• Textile Coloration and Finishing
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• v.15 no.1
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• pp.1-7
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• 2003

To study the dyeability(ΔE value) and color depth of silk fabrics dyed with Impatiens balsamina extracts. its flower, leaf and stem was extracted by water. This paper investigated some experiments under the various dyeing conditions such as dyeing temperature, dyeing time, repetition of dyeing, dyeing pH with silk fabrics dyed with Impatiens balsamina extracts. The results obtained were as follows 1. It was found that $\lambda_{max}$ of silk fabrics dyed with Impatiens balsamina extract was 260nm and its flower, leaf , stem and different colored flower(white, pint violet) had same $\lambda_{max}$ at 260nm. 2. $\Delta{E}$ values were increased when dyeing temperature, repetition of dyeing and time was higher, value of munsell of silk fabrics dyed with Impatiens balsamina extract was Yellow-Red. But dyeing temperature, repetition of dyeing and time was higher, shifted to longer wavelenth side. 3. Silk fabric dyed with Impatiens balsamina extracts at acidic pH was colored reddish Yellow-Red, alkali bath colored yellowish Yellow-Red in silk fabrics dyed with Impatiens balsamina extract.

• The Korean Journal of Pesticide Science
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• v.14 no.4
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• pp.394-400
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• 2010

Insecticidal activities of methanol extracts of the different parts of Prunus mume were investigated against the larvae of Cnaphalocrocis medinalis (C. medinalis) by topical application. The mortalities of the larvae of C. medinalis were 34% at the concentration of 4,000 ppm, 36% of 8,000 ppm, 40% of 16,000 ppm and 58% of 32,000 ppm of the extracts from the fruits, and 38% of 4,000 ppm, 45% of 8,000 ppm, 58% of 16,000 ppm and 75% of 32,000 ppm from the stem + flower of Prunus mume (P. mume), respectively. The rates of pupation of C. medinalis treated with either fruit or stem+flower were gradually low according to the concentration of extracts increased. The weight of pupae of C. medinalis has no significantly different in between the control and the treated groups of the extracts from both of fruit and stem+flower. However, the differences of the pupal weight and the rate of pupation of C. medinalis treated with the extracts from the stem+flower and fruit of P. mume were significant as the level of 56% ($R^2$=0.56363, P=0.4364). Both of stem+flower and fruit extracts of P. mume showed good effects of anti-feeding against C. medinalis. The extracts of the stem+flower of P. mume could be advocated for developing as birational agent for the control of C. medinalis.

• FLOWER RESEARCH JOURNAL
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• v.17 no.3
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• pp.158-164
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• 2009

Present studies were conducted to investigate the effects of water and 80% ethanol solvent on antioxidant activities of flower extracts of Chrysanthemum frutescens, Coreopsis lanceolata, Matricaria recutica, and Hieracium pilosella. Extraction yields of water extracts ranged 27.15~40.25% and 80% ethanol extracts 24.92~42.84%, respectively. In all species polyphenol and flavonoid contents were higher with 80% ethanol extraction, especially in C. lanceolata. Same results were obtained with scavenging effects on DPPH and ABTS radicals and ferrous ion chelating effects. Scavenging activity of flower extracts on DPPH radicals was highest in H pilosella - higher than that of synthetic antioxidant DHT. Higher scavenging activity on ABTS radicals was observed with M. recutica. Generally Compositae flower extracts exhibited higher scavenging activity on ABTS radicals than DPPH. Scavenging activity on ABTS radicals of M. recutica and P pilosella were superior to those of ascorbic acid and BHT. Ferrous ion chelating effects were much higher with H. pilosella flower extracts by 80% ethanol. Chelating effects of 4 species were much lower than those of EDTA. In conclusion, to develop natural antioxidant from above 4 Compositae species, 80% ethanol is recommended for efficient solvent to obtain maximum antioxidant isolation and activity.