• Journal of Applied Biological Chemistry
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• v.65 no.2
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• pp.107-111
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• 2022

Biological activities such as antioxidant, anticoagulant, and α-glucosidase inhibitory effects of 40% (v/v) ethanolic extract from black locust (Robinia pseudoacacia L.) flower were investigated. The polyphenol content of the black locust flower extract was 39.8±0.5 mg gallic acid equivalents/g. The flower extract represented antioxidant effects such as free radical, cationic radical, and nitrite scavenging abilities as well as reducing power. Also the flower extract inhibited α-glucosidase activity and common pathway in plasma coagulation system.

• Journal of Physiology & Pathology in Korean Medicine
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• v.29 no.4
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• pp.322-329
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• 2015

Cirsium japonicum var. ussuriense are often used in treatment of human diseases such as hemorrhage, blood congestion and inflammation in Korea. However, there was not report on the anti-obesity efficacy of water extracts from different organs of C. japonicum var. ussuriense. Here, we investigated the antioxidant effects of water extracts of flowers, leaves and roots from C. japonicum var. ussuriense. Total polyphenol amounts of the flower extract showed higher than those of leaf and root extract. Flower extract also showed the high antioxidant activities such as DPPH radical scavenging activity and reducing power. We also investigated the anti-obesity effects of water extracts of flowers, leaves and roots from C. japonicum var. ussuriense in 3T3-L1 cells and high fat diet-induced obesity mice. The mice were divided into four groups [high fat diet (HFD) control, HFD + leaf extract, HFD + flower extract and root extract] and administered with each extract (200 mg/kg) for 12 weeks. The flower and leaf extract significantly suppressed the levels of oil red O and triglyceride content. The flower and leaf extract also significantly reduced the triglyceride, total cholesterol and lower density lipoprotein levels of plasma as well as body and abdominal fat weight. Furthermore, oral glucose tolerance in the flower and leaf extract groups were significantly ameliorated in comparison to the high fat diet group. Therefore, these results indicate that the flower and leaf extract could ameliorate obesity and attenuate blood glucose level in high fat diet-induced obesity mice. We conclude that this study may provide positive insights into water extracts of flowers and leaves from C. japonicum var. ussuriense as a functional food ingredient for treatment of obesity.

• KSBB Journal
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• v.30 no.2
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• pp.77-81
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• 2015

The antioxidant and anti-inflammatory activities of extract and its fraction of Daucus carota var. sativa flower were studied in vitro. Extract and ethyl acetate fraction, butanol fraction of carrot flower showed radical scavenging effects on 1,1-diphenyl-2-picrylhydrazyl (DPPH). We also investigated the effect of extract and ethyl acetate fraction, butanol fraction of carrot flower on NO production in a lipopolysaccharide (LPS)-stimulated murine macrophage RAW 264.7 cells. Extract and its fraction of carrot flower significantly inhibited NO production and this inhibitory action was not due to the cytotoxicity. This study suggests that extract and ethyl acetate fraction, butanol fraction of Daucus carota var. sativa flower could contribute to the chemoprevention and therapy of oxidative stress and inflammation.

• Food Science and Biotechnology
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• v.17 no.1
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• pp.156-160
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• 2008

This study was conducted to evaluate the hypotensive properties of the extract of Chinese balloon flower (Platycodon grandiflorum)'s root. In the studies for calcium channel-blocking using Xenopus oocytes, the ethanol-extract ($26.2{\pm}5.2%$) showed higher activity than water-extract. Twenty female rats were fed 25, 35, and 45 mg/kg BW/day of the ethanol-extract for 14 days to observe the changes in blood pressures and heart pulses. Ethanol-extract decreased the systolic, diastolic, and mean blood pressures of the rats. Especially, the rats fed with 45 mg/kg BW/day of the ethanol-extract showed significant decreases in the blood pressures. These results suggested that a decrease in blood pressures was due to the extension of a blood vessel with calcium channel-blocking by ethanol-extract of Chinese balloon flower. Forty %-ethanol showed the highest efficiency for ethanol-extraction of Chinese balloon flower.

• Food Science and Preservation
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• v.6 no.1
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• pp.104-109
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• 1999

Ethyl acetate, methanol, water extract and their fractions from chestnut flower(Castancea crenata) were tested for antimicrobial activities. Yields of prebloomed chestnut flower extracts were 13.84, 12.90 and 1.82% in methanol, water and ethyl acetate, and those of the postbloomed were 13.12, 11.75 and 1.18%, respectively. Methanol extract from the chestnut flower was fractionated by solvents using hexane, chloroform, ethyl acetate, n-butanol and water, yields of those fractions were 0.16, 0.08, 1.94, 4.75 and 6.91% in the prebloomed, and were 0.90, 0.13, 1.40, 3.42 and 7.18% in the postbloomed. In the solvent extracts of water, ethyl acetate and methanol, methanol extract showed the most effective antimicrobial activity, antimicrobial activity of ethyl acetate fraction of methanol extract was stronger than others. Minimum inhibitory concentration of ethyl acetate fractions from the prebloomed showed 100, 140, 100 and 90ppm against Staphylococcus aureus, Streptococcus mutans, Echerichia coli and Salmonella typhimurium, in fractions from the postbloomed were 140, 140, 100 and 150ppm, respectively. Growth of all the strains was completely inhibited to 30 hours in a 150ppm concentration. E. coli sells treated with ethyl acetate fraction was collapsed severely.

• Journal of Life Science
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• v.27 no.9
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• pp.1059-1063
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• 2017

Angiogenesis is an essential step in tumoral growth and metastasis and is regulated by a balance between stimulators and inhibitors. Recently, antiangiogenic target therapy has shown promise as a new type of chemotherapy. Natural products have attracted widespread attention worldwide as a useful source of novel therapeutic compounds. The balloon-flower has long been used as a traditional medicinal material and food in Asia. In this study, we investigated whether extract of balloon-flower would inhibit in vitro angiogenesis and vascular-like network formation in human umbilical vein endothelial cells (HUVECs). The extract of Balloon-flower did not affect the viability of HUVECs. However, treatment with the Balloon-flower extract suppressed tube formation of HUVECs. In addition, after treatment with the Balloon-flower extract, cell migration decreased about 80%, and cell invasion was almost completely inhibited. Taken together, these results suggest that extract of Balloon-flower may have potential as an angiogenic inhibitor and that it could be developed as an anticancer agent.

• Food Science and Biotechnology
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• v.16 no.3
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• pp.409-414
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• 2007

Antimicrobial and antioxidative effects of 14 different herbal flower extracts on skin microorganisms and oxidation were tested in this research. Herbal flower extracts were prepared with 70% ethanol. Among the herbal flower extracts, roselle (Hibiscus sabdariffa L.) flower extract showed the highest antimicrobial activity against Staphylococcus epidermidis as determined by a paper disc method. The seventy % ethanol extract of roselle flower was fractionated by sequential hexane, chloroform, ethyl acetate, n-butanol, and water fractionation. The growth of S. epidermidis, Streptomyces collinus, Streptomyces coeruleoprunus, Salmonella enteritidis, Vibrio parahaemolyticus, and Malassezia pachydermatis was most efficiently inhibited by ethyl acetate fraction of roselle flower extract as determined by a paper disc method and growth inhibition curves. In addition, the ethyl acetate fraction, water fraction and butanol fraction showed free radical scavenging and DNA cleavage inhibition activities. These results demonstrate that roselle flowers hold antimicrobial and antioxidative activities against skin microorganisms and oxidants.

• Korean Journal of Plant Resources
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• v.25 no.5
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• pp.543-549
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• 2012

To gather the basic data for increasing the utilization of Isatis tinctoria, we examined the effects of both antioxidative enzyme activity and antimicrobial activity from the extract of Isatis tinctoria. Ascorbate Peroxidase activities reveal that there is an decrease in order; ethanol extract from its stem (1601.7 Unit/mg protein), methanol extract from its leaf (1133.7 Unit/mg protein) and distilled water extract from its leaf (524.3 Unit/mg protein). Catalase activities reveal that there is an decrease in order; ethanol extract from its flower petal (177.1 Unit/mg protein), methanol extract from its leaf (120.8 Unit/mg protein) and distilled water extract from its flower petal (55.4 Unit/mg protein). Peroxidase activities reveal that there is an decrease in order; ethanol extract from its flower petal (27.1 Unit/mg protein), methanol extract from its flower petal (14.6 Unit/mg protein) and distilled water extract from its stem (10.4 Unit/mg protein). Superoxide dismutase activities reveal that there is an increase in order; distilled water extract from its root (90.8%), methanol extract from its flower petal (80.1%) and ethanol extract from its root (75.5%). Its flower extract showed a antimicrobial activity only against Vibrio parahaemolyticus, its root extract had only against Staphylococcus aureus, and its stem extract had against Bacillus subtilis, Escherichia coli and Staphylococcus aureus, regardless of solvents. Especially, distilled water extract from its leaf showed a high antimicrobial activity against both Bacillus subtilis and Escherichia coli and inhibition diameters against those were 30.0 and 24.0 mm, respectively.

• Korean Journal of Plant Resources
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• v.31 no.3
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• pp.202-210
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• 2018

Abeliophyllum distichum is a medicinal plant used in regional traditional medicine to relieve pain in inflammatory processes. In this study, anti-inflammatory effects of Abeliophyllum distichum flower (ADF) extract were examined. Furthermore, possible molecular mechanisms of the anti-inflammatory effects were dissected. The anti-inflammatory activity was investigated by inhibition of lipopolysaccharide (LPS) induced pro-inflammatory cytokine production in murine macrophage-like cell line Raw264.7 cells. The measurement of the induced pro-inflammatory cytokine levels were carried out by ELISA. The phosphorylation of ERK1/2, JNK, and MAPK, and the nuclear expression of nuclear factor NF-${\kappa}B$ p65 were investigated by Western blot analysis. The extract of ADF significantly decreased the production of pro-inflammatory cytokines. In addition, the extract suppressed the phosphorylation of ERK1/2, JNK, and p38 MAPK, and the nuclear translocation of NF-${\kappa}B$ p65 in activated cells. Our findings provide evidence for the popular use of Abeliophylli distichum in inflammation around Goesan region and also suggest that the flower extract has potential therapeutic benefits against various inflammatory diseases.

• Korean journal of food and cookery science
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• v.27 no.1
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• pp.1-9
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• 2011

Anti oralmicrobial effects of Nulembo nucifera were determined against S.mutans, S.sobrinus S.sobrinus, F.nucleatum, and A.actinomycetemcomitans using paper disc method, minimum inhibitory concentrate (MICs). Extracts of lotus leaf showed the highest yield. MeOH extract is 21%, Chloroform fraction is 4.2%, ethylacetate fraction is 8.2%, water fraction is 19%. Different parts such as flower, leaf, seed and pod showed antimicrobial effects against S.mutans, with flower and seed extracts showing strong antimicrobial effect aganinst S.sobrinus KCCM11897. Leaf extract(1000pm concentration) showed over 50% inhibitory effect against S.mutans and S.sobrinus KCCM11897. Flower extract showed over 40% inhibitory effect against F.nucleatum and A.actinomycetemcomitans. MICs of flower extract against S.sobrinus KCCM11897,11898 and leaf extract against S.mutans, S.sobrinus KCCM11897 were $625\;{\mu}g/ml$, indicating Nulembo nucifera extract can exert antimicrobial activity even at low concentration. All extractes with heat at $120^{\circ}C$ had antimicrobial activity, which means that is a very stable substances. F.nucleatum and A.actinomycetemcomitans was stable against acid it had a trend that the more akali side was the lower acitivity.