Browse > Article
http://dx.doi.org/10.7732/kjpr.2018.31.3.202

Anti-inflammatory Effects of Abeliophyllum distichum Flower Extract and Associated MAPKs and NF-κB Pathway in Raw264.7 Cells  

Lee, Jin-Wook (Department of Biomedical Science, Jungwon University)
Kang, Yoon-Joong (Department of Biomedical Science, Jungwon University)
Publication Information
Korean Journal of Plant Resources / v.31, no.3, 2018 , pp. 202-210 More about this Journal
Abstract
Abeliophyllum distichum is a medicinal plant used in regional traditional medicine to relieve pain in inflammatory processes. In this study, anti-inflammatory effects of Abeliophyllum distichum flower (ADF) extract were examined. Furthermore, possible molecular mechanisms of the anti-inflammatory effects were dissected. The anti-inflammatory activity was investigated by inhibition of lipopolysaccharide (LPS) induced pro-inflammatory cytokine production in murine macrophage-like cell line Raw264.7 cells. The measurement of the induced pro-inflammatory cytokine levels were carried out by ELISA. The phosphorylation of ERK1/2, JNK, and MAPK, and the nuclear expression of nuclear factor NF-${\kappa}B$ p65 were investigated by Western blot analysis. The extract of ADF significantly decreased the production of pro-inflammatory cytokines. In addition, the extract suppressed the phosphorylation of ERK1/2, JNK, and p38 MAPK, and the nuclear translocation of NF-${\kappa}B$ p65 in activated cells. Our findings provide evidence for the popular use of Abeliophylli distichum in inflammation around Goesan region and also suggest that the flower extract has potential therapeutic benefits against various inflammatory diseases.
Keywords
Abeliophyllum distichum; Anti-inflammatory; Flower extract; NF-${\kappa}B$;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Aderem, A. and R.J. Ulevitch. 2000. Toll-like receptors in the induction of the innate immune response. Nature 406: 782-787.   DOI
2 Amalinei, C., I.-D. Caruntu and R.A. Balan. 2007. Biology of metalloproteinases. Rom J Morphol Embryol. 48:323-334.
3 Axtelle, T. and J. Pribble. 2001. IC14, a CD14 specific monoclonal antibody, is a potential treatment for patients with severe sepsis. J Endotoxin Res. 7:310-314.   DOI
4 Bendtzen, K. 1988. Special Issue: Second Meeting on Infection Immunity Elsinore, Denmark, 17-21 June 1988Interleukin 1, interleukin 6 and tumor necrosis factor in infection, inflammation and immunity. Immunol Lett. 19:183-192.   DOI
5 Bhattacharyya, A., S. Pathak, S. Datta, S. Chattopadhyay, J. Basu and M. Kundu. 2002. Mitogen-activated protein kinases and NF-kappaB regulate H. pylori-mediated IL-8 release from macrophages. Biochem J. 366:376-382.
6 Binetruy, B., T. Smeal and M. Karin. 1991. Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain. Nature 351:122-127.   DOI
7 Bosca, L., M. Zeini, P.G. Traves and S. Hortelano. 2005. Nitric oxide and cell viability in inflammatory cells: a role for NO in macrophage function and fate. Toxicology 208:249-258.   DOI
8 Festuccia, C., D. Giunciuglio, F. Guerra, I. Villanova, A. Angelucci, P. Manduca, A. Teti, A. Albini and M. Bologna. 1998. Osteoblasts modulate secretion of urokinase-type plasminogen activator (uPA) and matrix metalloproteinase-9 (MMP-9) in human prostate cancer cells promoting migration and matrigel invasion. Oncol Res. 11:17-31.
9 Coussens, L.M., B. Fingleton and L.M. Matrisian. 2002. Matrix metalloproteinase inhibitors and cancer-trials and tribulations. Science 295:2387-2392.   DOI
10 Farabegoli, F., A. Papi and M. Orlandi. 2011. (-)-Epigallocatechin-3-gallate down-regulates EGFR, MMP-2, MMP-9 and EMMPRIN and inhibits the invasion of MCF-7 tamoxifen-resistant cells. Biosci Rep. 31:99-108.   DOI
11 Fujiwara, N. and K. Kobayashi. 2005. Macrophages in inflammation. Curr Drug Targets Inflamm Allergy. 4:281-286.   DOI
12 Ghosh, S. and M.S. Hayden. 2008. New regulators of $NF-{\kappa}B$ in inflammation. Nat Rev Immunol. 8:837-848.   DOI
13 Grivennikov, S.I., F.R. Greten and M. Karin. 2010. Immunity, inflammation, and cancer. Cell 140:883-899.   DOI
14 Hoshino, K., O. Takeuchi, T. Kawai, H. Sanjo, T. Ogawa, Y. Takeda, K. Takeda and S. Akira. 1999. Cutting edge: Toll-like receptor 4 (TLR4)-deficient mice are hyporesponsive to lipopolysaccharide: evidence for TLR4 as the Lps gene product. J Immunol. 162:3749-3752.
15 Guo, H.-F. and M.-H. Wang. 2017. Anti-inflammatory and anti-cancer effect of Stachys affinis tubers. Korean J. Plant Res. 30:679-685.
16 Herlaar, E. and Z. Brown. 1999. p38 MAPK signalling cascades in inflammatory disease. Mol Med Today. 5:439-447.   DOI
17 Hibi, M., K. Nakajima and T. Hirano. 1996. IL-6 cytokine family and signal transduction: a model of the cytokine system. J Mol Med. 74:1-12.   DOI
18 Iwalewa, E., L. McGaw, V. Naidoo and J. Eloff. 2007. Inflammation: the foundation of diseases and disorders. A review of phytomedicines of South African origin used to treat pain and inflammatory conditions. Afri J Biotech. 6(25):2868-2885.   DOI
19 Hung, W.-C., W.-L. Tseng, J. Shiea and H.-C. Chang. 2010. Skp2 overexpression increases the expression of MMP-2 and MMP-9 and invasion of lung cancer cells. Cancer Lett. 288:156-161.   DOI
20 Ide, N. and B.H. Lau. 2001. Garlic compounds minimize intracellular oxidative stress and inhibit nuclear $factor-{\kappa}B$ activation. J Nutr. 131:1020S-1026S.   DOI
21 Johnson, G.L. and R. Lapadat. 2002. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science 298:1911-1912.   DOI
22 Lazarov, S., M. Balutsov and E. Ianev. 1999. The role of bacterial endotoxins, receptors and cytokines in the pathogenesis of septic (endotoxin) shock. Vutr Boles. 32:33-40.
23 Libby, P., P.M. Ridker and A. Maseri. 2002. Inflammation and atherosclerosis. Circulation 105:1135-1143.   DOI
24 Lee, E.-J., S.-Y. Kim, J.-W. Hyun, S.-W. Min, D.-H. Kim and H.-S. Kim. 2010. Glycitein inhibits glioma cell invasion through down-regulation of MMP-3 and MMP-9 gene expression. Chem Biol Interact. 185:18-24.   DOI
25 Lee, T.H., H.B. Kwak, H.-H. Kim, Z.H. Lee, D.K. Chung, N.-I. Baek and J. Kim. 2007. Methanol extracts of Stewartia koreana inhibit cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) gene expression by blocking NF-kappaB transactivation in LPS-activated RAW 264.7 cells. Mol Cells. 23:398-404.
26 Li, Q. and I.M. Verma 2002. $NF-{\kappa}B$ regulation in the immune system. Nat Rev Immunol. 2:725-734.   DOI
27 Nalla, A.K., B. Gorantla, C.S. Gondi, S.S. Lakka and J.S. Rao. 2010. Targeting MMP-9, uPAR, and cathepsin B inhibits invasion, migration and activates apoptosis in prostate cancer cells. Cancer Gene Ther. 17:599-613.   DOI
28 Ljung, T., S. Lundberg, M. Varsanyi, C. Johansson, P.T. Schmidt, M. Herulf, J.O. Lundberg and P.M. Hellstrom. 2006. Rectal nitric oxide as biomarker in the treatment of inflammatory bowel disease: responders versus nonresponders. World J Gastroenterol. 12:3386.   DOI
29 Majdalawieh, A. and H.-S. Ro. 2010. Regulation of IkappaBalpha function and NF-kappaB signaling: AEBP1 is a novel proinflammatory mediator in macrophages. Mediators Inflamm. 2010:823821.
30 Nair, V.D., T. Yuen, C.W. Olanow and S.C. Sealfon. 2004. Early single cell bifurcation of pro-and antiapoptotic states during oxidative stress. J Biol Chem. 279:27494-27501.   DOI
31 Natarajan, K., S.K. Manna, M.M. Chaturvedi and B.B. Aggarwal. 1998. Protein tyrosine kinase inhibitors block tumor necrosis factor-induced activation of nuclear $factor-{\kappa}B$, degradation of $I{\kappa}B{\alpha}$, nuclear translocation of p65, and subsequent gene expression. Arch Biochem Biophys. 352:59-70.   DOI
32 Ryan, G.B. and G. Majno. 1977. Acute inflammation. A review. Am J Pathol. 86:183.
33 Opal, S.M. and V.A. DePalo. 2000. Anti-inflammatory cytokines. Chest 117:1162-1172.   DOI
34 Park, G.H., J.H. Park, H.J. Eo, H.M. Song, M.H. Lee, J.R. Lee and J.B. Jeong. 2014. Anti-inflammatory effect of the extracts from Abeliophyllum distichum Nakai in LPS-stimulated RAW264. 7 cells. Korean J. Plant Res. 27:209-214.   DOI
35 Park, J.-H., B.-G. Lee, G.-I. Byun and D.-W. Kim. 2010. Antioxidant activities and inhibitory effect on oxidative DNA damage of Nelumbinis semen extracts. Korean J. Herbol. 25:55-59.
36 Pasparakis, M., L. Alexopoulou, V. Episkopou and G. Kollias. 1996. Immune and inflammatory responses in TNF alpha-deficient mice: a critical requirement for TNF alpha in the formation of primary B cell follicles, follicular dendritic cell networks and germinal centers, and in the maturation of the humoral immune response. J Exp Med. 184:1397-1411.   DOI
37 Ramos-DeSimone, N., E. Hahn-Dantona, J. Sipley, H. Nagase, D.L. French and J.P. Quigley. 1999. Activation of matrix metalloproteinase-9 (MMP-9) via a converging plasmin/stromelysin-1 cascade enhances tumor cell invasion. J Biol Chem. 274:13066-13076.   DOI
38 Sil, H., T. Sen and A. Chatterjee. 2011. Fibronectin‐Integrin (${\alpha}5{\beta}1$) Modulates migration and invasion of murine melanoma cell line B16F10 by involving MMP-9. Oncol Res. 19: 335-348.   DOI
39 Vallabhapurapu, S. and M. Karin. 2009. Regulation and function of $NF-{\kappa}B$ transcription factors in the immune system. Annul Rev Immunol. 27:693-733.   DOI
40 Visse, R. and H. Nagase. 2003. Matrix metalloproteinases and tissue inhibitors of metalloproteinases structure, function, and biochemistry. Circ Res. 92:827-839.   DOI
41 Xin, W., L. Wen-bo, L. Yan, Z. Jian and C. Ting-mei. 2013. Effect of osteoprotegerin in combination with interleukin-6 on inhibition of osteoclast differentiation. Chin J Traumatol. 16:277-280.