• 대한화장품학회:학술대회논문집
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• 대한화장품학회 2003년도 IFSCC Conference Proceeding Book II
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• pp.545-547
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• 2003

A tyrosinase inhibitor, Lonjapoin, was isolated from the ethanolic extracts of Flos Lonicera by activity-guided fractionation. Lonjapoin ($IC_{50}$/; 2.07 $\mu\textrm{g}$/mL) was found to be potentially as effective inhibitor of production of melamin. Flos Lonicera (Jinyinhua) is the dry flower buds of Lonicera japonica. Thunb. It is used as an antibacterial and antiphlogistic agent in the treatment of abscess, laryngeal catarrh, erysipelas, dysentery, cold and fever. In our continuing search for natural source as cosmetic ingredients from Chinese medicinal herbs and found the extracts of Flos Lonicera showed the significant tyrosinase inhibition activity. In this study the active constituent of Lonicera japonica. obtained with the process of tyrosinase activity assay and column chromatography.

• Natural Product Sciences
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• 제16권1호
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• pp.32-38
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• 2010

From the polar fractions of a 70% EtOH extract of the flower buds of Lonicera japonica (Caprifoliaceae), ten constituents were isolated and identified as iridoid glycosides 7-dehydrologanin (7-ketologanin, 2), secologanin dimethyl acetal (3), (E)-aldosecologanin (centauroside, 5), dimethyl secologanoside (6), secoxyloganin (7) and epivogeloside (8). Other identified constituents were 1-O-$\beta$-D-glucopyranosyl-(2S,3S,4R,8E/Z)-2-[(2R)-2-hydroxy(docosanoyl, tricosanoyl, tetracosanoyl, pentacosanoyl)amino]-8-octadecene-1,3,4-triol (1), uracil (4), D-mannitol (9), and sucrose (10). Among them, 1, 2, 4, and 10 were isolated for the first time from this plant.

• Natural Product Sciences
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• 제4권4호
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• pp.263-267
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• 1998

From the dried flower-buds of Syzygium aromaticum Merr. et Perry (Myrtaceae), seven compounds, i.e., eugenol (1), oleanolic acid (2), kaempferol 7-O-metylether (3), 3,3',4-tri-O-methylellagic acid (4), maslinic acid (5), ${\beta}-sitosterol-3-O-glucoside$ (6), and isorhamnetin 3-O-glucoside (7) were isolated. Compound 1 showed cyclooxygenase-2 (COX-2) inhibitory activity.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.260.1-260.1
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• 2003

Magnoliae Flos(‘shin-j’). the flower buds of Magnolia kobus, is acrid to taste with a ‘warm’ property. It is a ‘wind-cold’ discutient and nasal decongestant and is principally used in the treatment of nasal congestion with headache, sinusitis and allergic rhinitis. By screening inhibitory activities on the melanin polymer biosysthesis in B-16 mouse melanoma cell lines, methylene chloride extract of Magnoliae Flos was found to have inhibitory effect on melanin polymer biosynthesis. (omitted)

• 식물분류학회지
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• 제52권1호
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• pp.35-44
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• 2022

Clematis pseudotubulosa (Ranunculaceae), a new species from Gyeonggi-do in the Republic of Korea, is described and illustrated. The new species is morphologically similar to C. takedana but differs in its leaves, which are always ternate with shallowly lobed leaflets (vs. 1-pinnate with deeply lobed three to five leaflets), and its oblong-shaped flower buds (vs. ovoid), larger flowers (vs. smaller flowers), and pantoporate pollen (vs. tricolpate). A detailed species description, geographical distribution, and an identification key for all species of Clematis that occur in Korea are also provided.

• 원예과학기술지
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• 제17권3호
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• pp.333-336
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• 1999

사과 품종 '후지'와 '조나골드'의 2년생 가지상에 발생한 신초에 대하여 5월 초순부터 7월 중순까지 약 12일 간격으로 기부에 5엽을 남기고 각각 절단전정을 실시한 결과, 절단한 가지상에 2차 생장이 이루어졌으며 절단전정의 시기와 관계없이 '후지'가 '조나골드'에 비하여 더 왕성한 2차 생장을 나타내었다. 그러나 각 품종에서의 2차 생장은 절단전정의 시기에 따라 다르게 나타나 '후지'의 경우 5월 초순이나 7월에 실시하였을 때보다 5월 중순 또는 6월에 실시하였을 때 더 왕성하였으며 '조나골드'의 경우에는 5월 중순까지의 전정에서는 2차 생장이 전혀 일어나지 않았으며 5월 하순 이후의 전정에 의해 2차 생장을 나타내었다. 하계 절단전정에 의하여 형성 된 정화아로부터 이듬해에 개화와 착과도 이루어졌다. 대체적으로 '조나골드'에 비하여 '후지'가 절단전정에 의한 정화아 형성률이 높은 경향 이었으며 '후지'는 5월 하순, '조나골드'는 6월 중순에 절단전정을 실시하였을 때 가장 높은 정화아 형성률을 나타내었다. 형성된 단과지로부터의 개화는 두 품종 모두 비슷하였으나 착과율은 '조나골드'보다 '후지'에서 다소 높게 나타났으며 각 품종에서의 착과율은 하계 절단전정의 시기에 따라 달라지지 않는 경향이었다. 따라서 하계절단전정은 품종에 따라 그 생장 특성에 맞게 시기를 달리하여야 효과적임이 구명되었다.

• 한국산림과학회지
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• 제104권2호
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• pp.332-335
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• 2015

자낭균인 Taphrina wiesneri는 한국의 공원과 도로 주변에 주로 식재되는 왕벚나무에 빗자루병을 발생시킨다. 이 병원균은 생태적 특성이 잘 알려져 있지 않아 방제법 등을 개발하는데 어려움이 있다. 이번 연구에서는 빗자루 병원균의 월동부위를 조사하기 위해 빗자루 병징을 보이는 왕벚나무의 건전가지와 이병가지, 그리고 병징이 나타나지 않은 건전한 왕벚나무 가지에서 종 특이적 primer (TwITSF와 TwITSR)를 사용하여 월동부위를 조사하였다. 그 결과 빗자루병에 감염된 왕벚나무의 이병가지 뿐만 아니라 건전가지에서도 종 특이적인 PCR 증폭산물이 관찰되었으며, 그 이외의 건전한 왕벚나무와 감염목 주변의 다른 식물 종 샘플에서는 PCR 증폭산물이 관찰되지 않았다.

• 한국식품과학회지
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• 제9권1호
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• pp.24-30
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• 1977

인삼의 부위별(部位別) saponin조성(組成)에 대하여 검토하였으며 아울러 시판인삼차(市販人蔘茶)을 분석하여 saponin의 함량(含量) 및 분포형태를 검토하므로써 이들 제품의 품질을 추정하고자 하였다. (1) 백삼에는 타부위(他部位)보다 $2.6{\sim}6.6$배의 Ra(O)를 함유하였다. (2) 미삼(尾蔘), 피삼, 뇌두에는 $Rb_1$, $Rb_2$, Rc가 많았고 total saponin의 약 50%에 달하였다. (3) Rbc와 Rg(f)의 비(比)는 인삼잎이 0.64 : 1, 백삼이 2 : 1, 뇌두가 3 : 1, 인삼꽃이 3.2 : 1, 피삼이 5.8 : 1, 미삼이 7 : 1 정도였고 백삼은 미삼에 비하여 Rg(f)의 상대함량(相對含量)이 약 3배정도 많았다. (4) 13종(種)의 시판(市販)인삼차를 분석한 결과 panaxadiol계(系) saponin과 panaxatriol계(系) saponin의 비(比)는 $0.8{\sim}8\;:\;1$로 많은 차이가 있었다.

• 동의생리병리학회지
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• 제19권2호
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• pp.481-489
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• 2005

Lonicerae Flos has antibacterial effects against Staphylococcus aureus, streptococci, pneumococci, Bacillus dysenterii, Salmonella typhi, and paratyphoid. It is an antiviral agent. The herb has a cytoprotective effect against $CCl_{4}-induced$ hepatic injury. It has antilipemic action, interfering with lipid absorption from the gut. Nowadays this herb is used mainly in the treatment of upper respiratory infections, such as tonsillitis and acute laryngitis. It is also used in the treatment of skin suppurations, such as carbuncles, and to treat viral conjunctivitis, influenza, pneumonia, and mastitis. Lonicerae Flos is dried flower buds of Lonicera japonica, L. hypoglauca, L. confusa, or L. dasystyla. But, for the most part, we use whole plant of Lonicera japonica, as a flower bud of it. And, little is known of the original copy of effects of whole plant, except for the 'Bon-Cho-Gang-Mok', which is written the effects of flower of Lonicera japonica are equal to effects of leaves and branch of it. The present study was conducted to evaluate the effect of flower and whole plant of Lonicera japonica on the regulatory mechanism of cytokines, inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) for the immunological activities in Raw 264.7 cells. In Raw 264.7 cells stimulated with lipopolysaccharide (LPS) to mimic inflammation, flower and whole plant of Lonicera japonica water extracts inhibited nitric oxide production in a dose-dependent manner and abrogated iNOS and COX-2. Flower and whole plant of Lonicera japonica water extract did not affect on cell viability. To investigate the mechanism by which flower and whole plant of Lonicera japonica water extract inhibits iNOS and COX-2 gene expression, we examined the on phosphorylation of inhibitor ${\kappa}B{\alpha}$ and assessed production of $TNF-{\alpha}$, $interleukin-1{\beta}$ $(IL-1{\beta})$ and interleukin-6 (IL-6). Results provided evidence that flower and whole plant of Lonicera japonica inhibited the production of $IL-1{\beta}$, IL-6 and activated the phosphorylation of inhibitor ${\kappa}B{\alpha}$ in Raw 264.7 cells activated with LPS. These findings suggest that flower and whole plant of Lonicera japonica can produce anti-inflammatory effect, which may play a role in adjunctive therapy in Gram-negative bacterial infections, respectively.

• 동의생리병리학회지
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• 제22권6호
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• pp.1509-1517
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• 2008

The flowers and buds of Lonicera Flower (LF), are used in Korean herbal medicine for latent-heat-clearing, antipyretic, detoxicant and anti-inflammatory ailments. This plant is used worldwide for the treatment of many types of inflammatory disease including respiratory infections, diabetes mellitus, rheumatoid arthritis and play an important role in immune reaction. These pharmaceutical effects of LF looks like to be related to its antioxidant capacity and phytochemicals containing in LF. In this study, the antioxidant activity of extract from LF was studied in vitro methods by measuring the antioxidant activity by TEAC, measuring the scavenging effects on reactive oxygen species (ROS) [superoxide anion, hydroxyl radical] and on reactive nitrogen species (RNS) [nitric oxide and peroxynitrite] as well as measuring the inhibitory effect on $Cu^{2+}$ induced human LDL oxidation and the inhibitory effect on collagen induced platelet aggregation. The LF extracts were found to have a potent scavenging activity, as well as an inhibitory effect on LDL oxidation and on platelet aggregation. In conclusion, the LF extracts have anti-oxidative and anti-atherosclerotic effects in vitro system, which can be used for developing pharmaceutical drug against oxidative stress and atherosclerosis.