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Detection of Overwintering Sites Inhabited by Cherry Witches' Broom Pathogen Taphrina wiesneri with Species-specific PCR in Korea

PCR을 이용한 벚나무 빗자루병균(Taphrina wiesneri)의 월동부위 검출

  • Son, Su-Yeon (Division of Forest Insect Pest and Diseases, Korea Forest Research Institute) ;
  • Lee, Sun Keun (Division of Forest Insect Pest and Diseases, Korea Forest Research Institute) ;
  • Seo, Sang-Tae (Division of Forest Insect Pest and Diseases, Korea Forest Research Institute)
  • 손수연 (국립산림과학원 산림병해충연구과) ;
  • 이선근 (국립산림과학원 산림병해충연구과) ;
  • 서상태 (국립산림과학원 산림병해충연구과)
  • Received : 2014.10.15
  • Accepted : 2014.12.09
  • Published : 2015.06.30

Abstract

Taphrina wiesneri, a pathogen of cherry witches' broom, is highly pathogenic to Prunus yedoensis Matsumura which are widely planted in parks and streets in South Korea. In order to control the disease, it is crucial to know the life cycle of the fungus. We attempted to detect the fungus tentatively overwintering in shoots and branches of cherry trees both having witches' broom and healthy before flowering and leafing in spring using PCR with species-specific primer set (TwITSF and TwITSR). Genomic DNAs were extracted from the symptomatic and the asymptomatic shoots or branches. Results indicated that T. wiesneri is present in leaf buds and inner bark not only in symptomatic branches but also in the asymptomatic branches in diseased trees. However, the fungus was not detected in flower buds of the symptomatic trees and any samples of healthy trees.

자낭균인 Taphrina wiesneri는 한국의 공원과 도로 주변에 주로 식재되는 왕벚나무에 빗자루병을 발생시킨다. 이 병원균은 생태적 특성이 잘 알려져 있지 않아 방제법 등을 개발하는데 어려움이 있다. 이번 연구에서는 빗자루 병원균의 월동부위를 조사하기 위해 빗자루 병징을 보이는 왕벚나무의 건전가지와 이병가지, 그리고 병징이 나타나지 않은 건전한 왕벚나무 가지에서 종 특이적 primer (TwITSF와 TwITSR)를 사용하여 월동부위를 조사하였다. 그 결과 빗자루병에 감염된 왕벚나무의 이병가지 뿐만 아니라 건전가지에서도 종 특이적인 PCR 증폭산물이 관찰되었으며, 그 이외의 건전한 왕벚나무와 감염목 주변의 다른 식물 종 샘플에서는 PCR 증폭산물이 관찰되지 않았다.

Keywords

References

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