• 대한전기학회:학술대회논문집
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• 대한전기학회 1994년도 하계학술대회 논문집 C
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• pp.1512-1516
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• 1994

FLIC(Fluid in cell) method has been used for analyzing a cold gas flow field in an interrupter of a circuit treater since 1970s. In this paper FLIC method is applied to calculation of a cold gas flow and then combined with the simple are analysis method in order to investigate the flow field characteristics in a puffer type interrupter rated at 145kV 40kA. The results for a cold gas flow agree with the experimental measurement well. The other results such as pressure rise, arc temperature, post-arc current and so forth am also reasonable and show the possibility that the combined program can be applied to the design of UHV interrupters.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 1999년도 춘계 학술대회논문집
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• pp.85-89
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• 1999

Numerical Analysis has been done for the supersonic off-design jet flow due to the pressure difference between the jet and the ambient fluid. The difference of pressure generates an oblique shock or an expansion wave at the nozzle exit, The waves reflect repeatedly at the center axis and on the sonic surface in the shear layer, and the pressure difference is resolved across these waves interacted with the turbulence mixing layer. In this paper, the axi-symmetric Navier-Stokes equation has been used with two equation $k-{\varepsilon}$ turbulence closure model. The second order TVD scheme with flux limiters, based on the flux vector split by the smooth eigenvalue split, has been used to capture internal shocks and other discontinuities. The correction term for the compressible flow and the damping function are used in the turbulence model. Numerical calculations have been done to analyze the off-design jet flow due to the pressure difference. The variation of pressure along the flow axis is compared with an experimental result and other numerical result. The characteristics of the interaction between the shock cell and the turbulence mixing layer have been analyzed.

• Journal of Acupuncture Research
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• 제20권2호
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• pp.98-111
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• 2003

Objective : This study was designed to analyze the effects of bee venom and melittin on cell death in neuroblastoma cell line. Methods : MTT assay, morphologic method, DNA fragmentation, flow cytometry, immunocytochemistry analysis, RT-PCR and Western blot were performed. Results : The obtained results are summarized as follows: 1. The MTT assay demonstrated that neuroblastoma cell viability was significantly inhibitted dose-dependently by treatment with bee venom and melittin in comparison with control. 2. Cell culture demonstrated that control group proliferated highestly at he 5th day in comparison with the 4th day in bee venom and melittin group. And in bee venom and melitti group cell proliferation decreased 2.5 times than control group. 3. The morphologic study demonstrated that neuroblastoma cell showed apoptosis after treatment with bee venom and melittin for 6 hours using microscope. 4. The Flow cytometry demonstrated that apoptosis of neuroblastoma cell treated with bee venom and melittin was related with stop of cell cycle in stage of $G_0/G_1$. 5 .DNA fragmenation demonstrated that neuroblastoma cell treated with bee venom and melittin showed DNA ladder below 1 Kbp. 6. Immunocytochemistry assay demonstrated that Fos and MAPK which are related with cancer were down-regulated by treatment with bee venom and melittin. 7. RT-PCR analysis demonstrated that Fos and MAPK mRNA were transcripted. Fos was down-regulated form treatment with $5{\mu}g/ml$ bee venom and MAPK was down-regulated form $1{\mu}g/ml$ bee venom. 8. Western blot demonstrated that Fos was down-regulated from $1{\mu}g/ml$ bee venom whereas MAPK was expressed by $1{\mu}g/ml$ bee venom but down-regulated by $10{\mu}g/ml$ bee venom. Conclusions : We found that some cancer related genes ware down-regulated by treatment with bee venom and melittin. Further study is needed for investigating the anti-cancer effect of bee venom and melittin.

• Applied Microscopy
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• 제27권2호
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• pp.121-130
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• 1997

It has been demonstrated that majority of cells in the mammalian body such as myocytes and epithelial cells of skin and intestine respond to mechanical force or environmental factors and exhibit partial disruption of cell membrane, i. e., cell wounding, even in a physiological condition. Myocardial cells are rather apt to be wounded than other cells since they are definitely exposed to mechanical stress by contraction-relaxation and blood flow. However, the mechanism how myocardial cells protect themselves against cell wounding is not yet clarified. On this background, the present study was performed to elucidate whether albumin leakage is related to cell wounding and to assess whether diltiazem, a potent calcium channel blocker, is beneficial in isoproterenol-induced cell wounding in the heart. Hearts isolated from New Zealand White rabbits ($1.5\sim2.0kg$ body weight, n=20) were perfused with Tyrode solution by Langendorff technique. After stabilization of baseline hemodynamics, the hearts were subjected to bolus administration of isoproterenol and diltiazem as following order: $1.6{\mu}M$ isoproterenol at zero min (the beginning point): $16{\mu}M$ diltiazem at 20min; $1.6{\mu}M$ isoproterenol at 25min; $16{\mu}M$ isoproterenol at 45 min; $160{\mu}M$ diltiazem at 65 min; $16{\mu}M$ isoproterenol at 70 min. During all experiments, the left ventricular function was recorded, albumin leakage in the coronary effluents was analyzed by electrophoresis and Western blot, and myocardial cell membranes were examined by conventional transmission electron microscopy. Data were analyzed by t-test and linear regression test. Isoproterenol significantly increased the inotropic and chronotropic contractions, coronary flow, and frequency of arrhythmia, however, diltiazem did not influence on hemodynamics except decrease in the frequency of arrhythmia and a slight decrease in contractility. Isoproterenol also resulted partial disruption of myocardial cell membrane and inclose in albumin leakage, while diltiazem pretreatment showed number of electron-dense plaques in the cell membrane and a tendency of decrease in albumin leakage. These results indicate that albumin leakage may be an indirect index of cell wounding in the heart and diltiazem nay be beneficial to protect myocardial cells against isoproterenol-induced cell wounding. It is likely that diltiazem promotes resealing process of the cell membrane.

• 한국수소및신에너지학회논문집
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• 제30권6호
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• pp.523-530
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• 2019

For the low-Pt electrodes for polymer electrolyte fuel cells (PEMFCs), the optimization of ionomer content for anode catalyst layers was carried out. A commercial catalyst of 20 wt.% Pt/C was used instead of 50 wt.% Pt/C which is commonly used for PEMFCs. The ionomer content varies from 0.6 to 1.2 based on ionomer to carbon ratio (I/C) and the catalyst layer is formed over the electrolyte by the ultrasonic spray process. Evaluation of the prepared MEA in the unit cell showed that the optimal ionomer content of the air electrode was 0.8 on the I/C basis, while the hydrogen electrode was optimal at the relatively high ionomer content of 1.0. In addition, a large difference in cell performance was observed when the ionomer content of the hydrogen electrode was changed. Increasing the ionomer content from 0.6 to 1.0 by I/C in a hydrogen electrode with 0.05 mg/㎠ platinum loading resulted in more than double cell performance improvements on a 0.6 V. Through the analysis of various electrochemical properties in the single cell, it was assumed that the change in ionomer content of the hydrogen electrode affects the water flow between the hydrogen and air electrodes bounded by the membrane in the cell, which affects the overall performance of the cell. A more specific study will be carried out to understand the water flow mechanism in the future, and this study will show that the optimization process of hydrogen electrode can also be a very important cell design variable for the low-Pt and high-performance MEA.

• 대한방사성의약품학회지
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• 제8권2호
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• pp.53-61
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• 2022

This study aimed to increase the targeting ability against PSMA in cell therapy using metabolic glycoengineering and biorthogonal chemistry and to visualize cell trafficking using PET imaging. Cellular membranes of THP-1 cells were decorated with azide(-N₃) using Ac₄ManNAz by metabolic glycoengineering. Engineered THP-1 cells were conjugated with DBCO-bearing fluorophore (ADIBO-Cy5.5) for 1 h at different concentrations and analyzed by confocal fluorescence microscopy and flow cytometry. For PSAM ligand conjugation to THP-1 cells, Ac₄ManNAz treated THP-1 cells were incubated with DBCO-PSMA ligand (ADIBO-GUL) at a final concentration with 100 µM for 1 h. To evaluate the effect on cell recognition, PSMA ligand conjugated THP-1 cells(as effectors) were co-cultured with PSMA positive 22RV1 (as target cells) at 3 : 1 a effector-to-target cell (E/T) ratio. The interaction between THP-1 and 22RV1 was monitored by confocal fluorescence microscopy. For preparing the radiolabeled THP-1, the cells were treated at the activity of ~ 740 kBq of [⁸⁹Zr]Zr(oxinate)₄/5 × 10⁶ cells. Radiolabeled cells were analyzed for determination of cell-associated radioactivity by gamma counting and viability using MTS assay. In the cytotoxicity assay, THP-1 cells did not have any cytotoxicity even when the Ac₄ManNAz concentration was 100 µM. In confocal microscopy and flow cytometry, THP-1 cells were efficiently labeled ADIBO-Cy5.5 in a dose-dependent manner, and the dose of 100 µM was the optimal concentration for the following experiments. The clusters of PSMA ligand-conjugated THP-1 cells and 22RV1 cells were identified, indicating cell-cell recognition over the cell surface between two types of cells. Cell radiolabeling efficiency was 54.5 ± 17.8%. THP-1 labeled with 0.09 ± 0.03 Bq/cell showed no significant cytotoxicity compared to unlabeled THP-1 up to 7 days. We successfully demonstrated that Ac₄ManNAz treated cells were efficiently conjugated with ADIBO-GUL for preparing the PSMA-targeting cells, and [⁸⁹Zr]Zr(oxinate)₄ could be used to label cells without toxicity. It suggested that PSMA-ligand conjugated cell therapy could be improved cell targeting and be monitored by PET imaging.

• 동의생리병리학회지
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• 제17권1호
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• pp.209-212
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• 2003

A wide variety of cancer chemotherapeutic agents have been shown to induce programmed cell death (PCD, apoptosis) in various tumor cell fines in vitro. This study was performed to know how ginsenoside Rc affect on SK-MEL-28 cell line, and how they induce the apoptosis. SK-MEL-28 cell lines were treated with various concentrations of ginsenoside Rc and cultured for various times. At cell cycle analysis, cells arrested at G2/M phase by ginsenoside Rc and apotosis percentage increased along with increasing concentration and time. TUNEL assay was performed to know whether SK-MEL-28 cell fine die as apoptosis or necrosis by ginsenoside Rc. As a result, fluorescence increased along with increasing time and concentration. Fas expressed on SK-MEL-28 cell lines membrane by ginsenoside Rc was identified using flow cytometer. Ginsenoside Rc induced apoptosis against SK-MEL-28 cell fines, and the apoptosis mechanism was identified as Fas-mediated apotosis.

• 에너지공학
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• 제11권3호
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• pp.254-261
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• 2002

The fabrication method for the main components of the polymer electrolyte membrane fuel cell stack such as electrodes, membrane-electrode assemblies, and bipolar plates was established for the effective electrode area of 240 ㎠. A counter-flow type 100-cell stack was fabricated by using the above components and then a maximum power of 7.44 kW for H$_2$/O$_2$ and 5.56 kW for H$_2$/air could be obtained at 70$\^{C}$ and 1 atm. It was seen that the distribution of the OCV for unit cells in the stack was uniform but the voltage deviation increased as the load increased due to the IR drop and the electrode polarization. The stack was applied to the power source of the fuel cell/battery hybrid electric golf car. It produced about 1 kW at a room temperature operation during the test run, which occupied about 43% of the total power required by the 2.3 kW motor.

• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.6429-6433
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• 2012

To explore a possible new treatment for human ovarian cancer, we studied the effects of sodium valproate on the growth of the HO8910 human cell line. HO8910 cells were cultured in vitro and treated with different concentrations of sodium valproate. Cell proliferation, cell cycling, and apoptosis were measured by flow cytometry, cell morphology under a microscope, and expression levels of WWOX and P27 by Western blotting and RT-PCR. Tumor xenografts were established to determine in vivo effects of sodium valproate. Our results showed that cell proliferation was decreased with increasing concentration of sodium valproate, with features of cytoplasmic retraction and floating cells. Moreover, cell cycle analysis revealed a higher apoptosis rate and $G_0/G_1$ phase in the sodium valproate experimental group than in the control group. In addition, protein expression levels of WWOX and P27 were elevated. Importantly, sodium valproate decreased in vivo xenograft tumor burden and up-regulated WWOX and P27 expression in nude mice. In conclusion, sodium valproate might play a role in inhibition and control of ovarian cancer cell line HO8910 by inhibiting cell proliferation, interfering with the cell cycle and promoting apoptosis, so that it may be effective in the clinical treatment of ovarian cancer.

• 한국자동차공학회논문집
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• 제23권1호
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• pp.49-55
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• 2015

In this study, among in various scenarios of the duration degradation of the fuel cell, countermeasures for the cathode carbon carrier oxidation and the deactivation of catalyst by hydrogen / air interface formation have been studied. so the system was applied to the air cutoff valve. In terms of the component, the cold start performance, electrical stability, the airtight performance were mainly designed and their performance was confirmed. And in terms of the system, the air electrode flow is blocked off, so the oxygen concentration drops when system is powered off, As a result, By reducing unit cell voltage which affect the durability of the fuel cell reached up to 0.8V, the improved durability of the fuel cell was confirmed.