Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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The Effects of Bee Venom & Melittin on Cell Death in Neuroblastoma Cell Line

Bee Venom 및 Melittin 약침액(藥鍼液)이 신경아세포종(神經芽細胞腫)의 세포사(細胞死)에 미치는 영향(影響)

  • Kang, Dong-cheol (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University) ;
  • Jung, Tae-young (Je-Han Oriental Medical Academy) ;
  • Seo, Jung-chul (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University) ;
  • Leem, Seong-cheol (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University) ;
  • Han, Sang-won (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-San University)
  • 강동철 (경산대학교 부속한방병원 침구과) ;
  • 정태영 (제한동의학술원) ;
  • 서정철 (경산대학교 부속한방병원 침구과) ;
  • 임성철 (경산대학교 부속한방병원 침구과) ;
  • 한상원 (경산대학교 부속한방병원 침구과)
  • Received : 2003.02.18
  • Accepted : 2003.03.22
  • Published : 2003.04.20
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Abstract

Objective : This study was designed to analyze the effects of bee venom and melittin on cell death in neuroblastoma cell line. Methods : MTT assay, morphologic method, DNA fragmentation, flow cytometry, immunocytochemistry analysis, RT-PCR and Western blot were performed. Results : The obtained results are summarized as follows: 1. The MTT assay demonstrated that neuroblastoma cell viability was significantly inhibitted dose-dependently by treatment with bee venom and melittin in comparison with control. 2. Cell culture demonstrated that control group proliferated highestly at he 5th day in comparison with the 4th day in bee venom and melittin group. And in bee venom and melitti group cell proliferation decreased 2.5 times than control group. 3. The morphologic study demonstrated that neuroblastoma cell showed apoptosis after treatment with bee venom and melittin for 6 hours using microscope. 4. The Flow cytometry demonstrated that apoptosis of neuroblastoma cell treated with bee venom and melittin was related with stop of cell cycle in stage of $G_0/G_1$. 5 .DNA fragmenation demonstrated that neuroblastoma cell treated with bee venom and melittin showed DNA ladder below 1 Kbp. 6. Immunocytochemistry assay demonstrated that Fos and MAPK which are related with cancer were down-regulated by treatment with bee venom and melittin. 7. RT-PCR analysis demonstrated that Fos and MAPK mRNA were transcripted. Fos was down-regulated form treatment with $5{\mu}g/ml$ bee venom and MAPK was down-regulated form $1{\mu}g/ml$ bee venom. 8. Western blot demonstrated that Fos was down-regulated from $1{\mu}g/ml$ bee venom whereas MAPK was expressed by $1{\mu}g/ml$ bee venom but down-regulated by $10{\mu}g/ml$ bee venom. Conclusions : We found that some cancer related genes ware down-regulated by treatment with bee venom and melittin. Further study is needed for investigating the anti-cancer effect of bee venom and melittin.

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