• Journal of Oriental Neuropsychiatry
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• v.11 no.1
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• pp.37-46
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• 2000

We investigated the effects of lemon pure essential oils on the heat shock-induced apoptosis in human astrocyte cell line CCF-STTG1. In previous studies, hear shock has been reported to induce the apoptosis or programmed cell death through the activation of caspase-3. Treatment of CCF-STTG1 cells with heat shock markedly induced apoptotic cell death as determined by flow cytometry. Interestingly, pretreatment of CCF-STTG1 cells with lemon pure essential oils inhibited the heat shock-induced apoptosis. Lemon also inhibited the heat shock-induced apoptosis in primary cultured rat astrocytes. To determine whether lemon inhibits the heat shock-induced activation of these apoptotic proteases, activation of CPP32 was assessed by Western blotting. Consistent with flow cytometry, DNA fragmentation and giemsa staining, heat shock-induced activation of CPP32 was blocked by lemon pure essential oil. PARP, cysteine protease substrates were fragmented as a consequence of apoptosis by heat shock. Lemon oil inhibited the PARP fragmentation. This essential oil also inhibited the heat shock-induced activation of caspase-3. These results suggest that lemon pure essential oils may modulate the apoptosis through the activation of the ICE-like caspases.

• YAKHAK HOEJI
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• v.34 no.3
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• pp.161-165
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• 1990

An automated, simple, and reliable method was developed for determining in vitro drug release rate from transdermal delivery dosage forms. The patch is held in position in the heating block by sandwiching it between the middle plate and the bottom plate of diffusion cell. The dissolution profile of the commercially available transdermal scopolamine patch was determined over a 72-h period, and the results were compared with those obtained with other methods; paddle-over-disk method, reciprocating method, and diffusion cell method. It was demonstrated that the flow-through method is equivalent in terms of release rate profile and accumulated released drug amount over the lifetime of the dosage form tested. Also this method is simple, reliable and reproducible. Therefore, this technique can be used in a quality control for assuring product uniformity.

• Korean Journal of Veterinary Research
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• v.39 no.6
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• pp.1112-1118
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• 1999

Galectin-3 plays an important role in cell development, differentiation and cancer metastasis, including cell-cell/extracellular matrix (ECM) interactions and is supposed to have an effect of apoptosis on T-cells in thymic clonal selection. In this study, to know the effect of galectin-3 on thymocyte development, we used recombinant human galectin-3 (rHgal-3) from Escherichia coli, JM105, which was inserted with human gal-3 gene-transformed plasmid vector (prGal-3) to express human galectin-3. Expressed rHgal-3 was confirmed by western blot using the culture supernant of hybridoma (M3/38) producing monoclonal antibody to human galectin-3. Sepharose gel affinity chromatography was used to purify the expressed rHgal-3. Thymocytes and hepatocytes from 6-week-old male BALB/c mice were incubated with rHgal-3 and showed marked increase of apoptotic population on analysis using flow cytometry with 7-AAD in a dosedependent manner. However, rHgal-3 failed to induce apoptosis on hepatocytes. Interestingly, this apoptotic effect was not inhibited by lactose, a specific lectin domain inhibitor. From these results, we concluded that extrinsic -3 induces apoptosis on mouse thymocytes, and galectin-3 may have an apoptotic effect on T-cells in thymic clonal selection.

• Journal of Electrochemical Science and Technology
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• v.2 no.1
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• pp.20-25
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• 2011

This work focuses on the behavior of the overpotential increase due to a utilization rise in a molten carbonate fuel cell. The behavior is generally explained by Nernst loss, which is a kind of voltage loss due to the thermodynamic potential gradients in a polarization state due to the concentration distribution of reactant species through the gas flow direction. The evaluation of Nernst loss is carried out with a traditional experimental method of constant gas utilization (CU). On the other hand, overpotential due to the gas-phase mass-transport resistance at the anode and cathode shows dependence on the utilization, which can be measured using the inert gas step addition (ISA) method. Since the Nernst loss is assumed to be due to the thermodynamic reasons, the voltage loss can be calculated by the Nernst equation, referred to as a simple calculation (SC) in this work. The three values of voltage loss due to CU, ISA, and SC are compared, showing that these values rise with increases in the utilization within acceptable deviations. When we consider that the anode and cathode reactions are significantly affected by the gas-phase mass transfer, the behavior strongly implies that the voltage loss is attributable not to thermodynamic reasons, namely Nernst loss, but to the kinetic reason of mass-transfer resistance in the gas phase.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2008.11a
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• pp.411-415
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• 2008

This paper presents on design factor considered in an altitude test cell facility to determine the best sizing to optimize exhaust diffuser pressure recovery and the exact cooling load required to be supplied under transient operation. Engine simulation was performed to analyse the exhaust gas temperature, exit mass flow rate, specific fuel consumption and exhaust velocity helpful in determining secondary mass air flow and the mixed air temperature entering the ejector. based on this, the amount of cooling load was deduced. It was found that improved pressure recovery reduces operational cost(air supply facility, cooling water).

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2008.11a
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• pp.31-35
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• 2008

This test cell is developed to provide the fundamentals of operational mechanism and structural configuration, and further to verify thermodynamic calculation with this test data to the institutes or laboratories research and study gas turbine engine for academic purpose. The test cell is installed to monitor and collect real-time data as to temperature, pressure, thrust, fuel flow, and air flow etc. using by NI DAQ(Data acquisition)device and LabVIEW program based on 30lbf-micro turbojet engine.

• BioChip Journal
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• v.12 no.4
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• pp.257-267
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• 2018

Inertial microfluidics has attracted significant attention in recent years due to its superior benefits of high throughput, precise control, simplicity, and low cost. Many inertial microfluidic applications have been demonstrated for physiological sample processing, clinical diagnostics, and environmental monitoring and cleanup. In this review, we discuss the fundamental mechanisms and principles of inertial migration and Dean flow, which are the basis of inertial microfluidics, and provide basic scaling laws for designing the inertial microfluidic devices. This will allow end-users with diverse backgrounds to more easily take advantage of the inertial microfluidic technologies in a wide range of applications. A variety of recent applications are also classified according to the structure of the microchannel: straight channels and curved channels. Finally, several future perspectives of employing fluid inertia in microfluidic-based cell sorting are discussed. Inertial microfluidics is still expected to be promising in the near future with more novel designs using various shapes of cross section, sheath flows with different viscosities, or technologies that target micron and submicron bioparticles.

• Journal of Veterinary Clinics
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• v.40 no.4
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• pp.298-302
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• 2023

A 7-year-old neutered male, domestic shorthair cat presented anorexia and lethargy. The complete blood cell count revealed severe non-regenerative anemia, lymphocytic leukocytosis, neutropenia, and thrombocytopenia. On the peripheral blood smear examination, medium to large lymphoblastic cells with moderate amounts of basophilic cytoplasm were observed in up to 70% of peripheral leukocytes. Feline leukemia and immunodeficiency viruses were not detected using a commercial diagnostic kit. While splenomegaly and blunt margins of the caudoventral liver were observed in abdominal radiography, changes in the intra-abdominal lymph nodes were not remarkable. Ultimately, flow cytometric immunophenotyping from the peripheral blood revealed a negative for B-cell markers (CD21-/CD79a-) and T-cell markers (CD3-/CD4-/CD5-/CD8-). Based on the hematological examination and the immunophenotyping assay, the cat was diagnosed with non-B, non-T acute lymphoblastic leukemia. Here, we report a rare case of non-B, non-T acute lymphoblastic leukemia to raise awareness and provide information on clinical symptoms and laboratory test and immunophenotyping analysis results.

• Transactions of the Korean Society of Automotive Engineers
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• v.17 no.2
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• pp.150-158
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• 2009

A centrifugal turbo blower is one of the part to generate electric power of fuel cell electric vehicle(FCEV). In order to generate the electric power of FCEV, the centrifugal turbo blower operates at very high speed above 30,000rpm in order to increase the pressure of the air, which supplied to a stack of FCEV, using rotation of its impeller blades. Vibration which originated from the blower is generated by unbalance of mechanical components, rotation of bearings and rotating asymmetry that rotate at high speed. The vibration is transmitted to receiving structure through vibration isolators and it can causes serious problems in the noise, vibration and harshness(NVH) performance. Thus, the study about reducing this kind of vibration is an important task. Quantifying the effectiveness of vibration isolation can be effectively accomplished by using vibrational power flow because relative contributions of each isolator to the total vibration transmission can be easily represented. In this paper, vibrational power flow is applied to the centrifugal turbo blower mounted on FCEV in order to analyze the most dominant vibration transmitting path. As a result, the main contributor among four isolators is a mount #3 of the blower. Also, a 30 percent lowering of the mount #3 stiffness shows 34 percent decrement of vibrational power flow by the simulation.

• Nuclear Engineering and Technology
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• v.16 no.2
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• pp.47-57
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• 1984

Mathematically-rigorous time-volume averaged conservation equations were simplified to established the differential equations of THERMIT-6S, which is a two-fluid 3-D code. The difference equations of THERMIT-6S were obtained by discretizing the proceeding set of differential equations. The spatial discretization is characterized by a first-order spatial scheme, donor cell method, and staggered mesh layout. For time discretization, a first order semi-implicit scheme treats implictly sonic terms and terms relating to local transport phenomena and explicitly convective terms. The results were linearized by the Newton-Raphson method. In order to construct the reduced pressure equation, the linearized equations were manipulated so that all variables are coupled between mesh cells through only the pressure variable. By simulating numerically the OPERA-15 experiment, it was found that THERMIT-6S is a very powerful code in predicting reactor behavior after sodium boiling including flow coastdown, reversal flow and flow oscillation.