Journal of Oriental Neuropsychiatry (동의신경정신과학회지)

The Korean Society of Oriental Neuropsychiatry (대한한방신경정신과학회)
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Inhibitory Effect of Lemon Oil on Apoptosis in Astrocytes

신경교(神經膠) 성상세포(星狀細胞)에서 레몬오일에 의한 세포자멸사(細胞自滅死)의 억제효과(抑制效果)

  • Kim, Jun-Han (Dept. of Neuropsychiatry, College of Oriental Medicine, Won Kwang University) ;
  • Kim, Tae-Heon (Dept. of Neuropsychiatry, College of Oriental Medicine, Won Kwang University)
  • 김준한 (원광대학교 한의과대학 신경정신과학교실) ;
  • 김태헌 (원광대학교 한의과대학 신경정신과학교실)
  • Published : 2000.09.30
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Abstract

We investigated the effects of lemon pure essential oils on the heat shock-induced apoptosis in human astrocyte cell line CCF-STTG1. In previous studies, hear shock has been reported to induce the apoptosis or programmed cell death through the activation of caspase-3. Treatment of CCF-STTG1 cells with heat shock markedly induced apoptotic cell death as determined by flow cytometry. Interestingly, pretreatment of CCF-STTG1 cells with lemon pure essential oils inhibited the heat shock-induced apoptosis. Lemon also inhibited the heat shock-induced apoptosis in primary cultured rat astrocytes. To determine whether lemon inhibits the heat shock-induced activation of these apoptotic proteases, activation of CPP32 was assessed by Western blotting. Consistent with flow cytometry, DNA fragmentation and giemsa staining, heat shock-induced activation of CPP32 was blocked by lemon pure essential oil. PARP, cysteine protease substrates were fragmented as a consequence of apoptosis by heat shock. Lemon oil inhibited the PARP fragmentation. This essential oil also inhibited the heat shock-induced activation of caspase-3. These results suggest that lemon pure essential oils may modulate the apoptosis through the activation of the ICE-like caspases.

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References

  1. 皮膚科學 大韓皮膚科學會刊行委員會
  2. 成人病, 老人病學 徐舞圭
  3. 東醫精神醫學 黃義完(外)
  4. 黃帝內經素問解釋 洪元植(譯)
  5. 黃帝內經靈樞解釋 洪元植(譯)
  6. 이철완교수의 老人病 硏究 이철완
  7. Annu. Rev.Cell. Biol. v.7 Ellis, R.E.;Yuan, J.;Horvitz, H.R.
  8. Science v.267 Steller, H.
  9. Essential Oil Safety Robert Tisserand;Tony Balacs
  10. 아로마테라피 이세희
  11. 中藥大辭典 江蘇新醫學院
  12. 韓國生化學會 v.5 no.3 老化의 메카니즘과 硏究方向, 生化學뉴스 崔鎭浩
  13. 대한정신약물학회지 v.3 no.1 痴呆의 原因과 治療 박종한
  14. Pathophysiology;adaptation and alterations infunction Bullock, B.L.
  15. 대한신경과학회지 v.3 no.1 神經科 領域에서의 痴呆 박영춘
  16. 慶熙醫學 v.13 no.1 痴呆 장대일
  17. Merritt's Textbook of Neurology Rowland, L.P.
  18. 精校黃帝內經靈樞 洪元植(編)
  19. 張氏景岳全書 張介賓
  20. 辨證奇門全書 錢鏡湖
  21. 國譯石室秘錄 陳士鐸
  22. 辨證錄 陳士鐸
  23. 編註醫學入門(卷二) 李천
  24. 古今醫鑑 공信
  25. 增補萬病回春 공廷賢
  26. 醫宗必讀 李中梓
  27. 備急千急要方(卷四十) 遜思邈
  28. 東醫神經精神科學會誌 v.8 no.2 Alzheimer型 痴呆患者 2例에 對한 臨床的 考察 金保岡(外)
  29. 韓醫學硏究所論文集 痴呆의 治療에 관한 東西醫學的 考察 李東垣(外)
  30. 大韓韓方內科學會誌 v.13 no.2 老人性痴呆에 대한 文獻的 考察 金賢兒
  31. 大韓韓醫學會誌 v.23 no.2 老人性 痴呆에 관한 體質醫學的 硏究 裵旿星
  32. 慶熙醫學 v.12 no.2 痴呆에 關한 韓醫學的 臨床 硏究 黃義完(外 4人)
  33. 慶熙醫學 v.13 no.1 痴呆治療에 대한 韓醫學的 接近方法 黃義完
  34. 大韓韓方內科學會 v.13 no.2 老人性 痴呆에 대한 文獻的 考察 이원철(外 2人)
  35. 동의신경과학회지 v.7 no.1 痴呆에 對한 文獻的 考察 鄭仁哲;李相龍
  36. 補腎活血化痰法治療老年性痴呆 32例 v.29 no.5 徐恒旺
  37. MERIDIAN v.6 no.1 Essential Oil흡입에 대한 EAV측정치의 변화연구-Aromatherapy의 임상적용을 위한 예비연구 오홍근
  38. J. Cell. Physiol. v.151 Mosser, D.D.;Martin, L.H.
  39. Int. J. Cancer v.66 Yonezawa, M.;Otsuka, T.;Matsui, N.;Kato, T.
  40. Biochem. Biophys. Res. Commun. v.241 John, D.R.;Kaushik, D.;James, P.K.
  41. FEBS Lett. v.324 Dubois, M.F.;Bensaude, O.
  42. J. Biol. Chem. v.272 Lin, R.Z.;Hu, Z.W.;Chin, J.H.;Hoffman, B.B.
  43. Science v.267 Thompson, C.B.
  44. Cell v.75 Yuan, J.;Shaham, S.;Ledoux, S.;Ellis, H.M.;Horvitz, H.R.
  45. Cell v.75 Miura, M.;Zhu, H.;Rotello, R.;Hartweig, E.A.;Yuan, J.
  46. J. Biol. Chem. v.269 Fernandes, A.T.;Litwack, G.;Alnemri, E.S.
  47. Genes Dev. v.8 Kumar, S.;Kinoshita, M.;Noda, M.;Copeland, N.G.;Jenkins, N.A.
  48. EMBO J. v.14 Faucheu, C.;Diu, A.;Chan, A.;Blanchet, A.M.;Miossec, C.;Herve, F.;Collarddutilleul, V.;Gu, Y.;Aldape, R.A.;Lippke, J.A.;Rocher, C.;Su, M.;Livingston, D.J.;Hercend, T.;Lalanne, J.L.
  49. Cancer Res. v.55 Ferandes-Alnemri, T.;Litwack, G.;Alnemri, E.S.
  50. J. Biol. Chem. v.270 Munday, N.;Vaillancourt, J.P.;Ali, A.;Casano, F.J.;Miller, D.K.;Molineaux, S.M.;Yamin, T.T.;Yu, V.L.;Nicholson, D.W.
  51. Nature v.376 Nicholson, D.W.;Ali, A.;Thornberry, N.A.;Vaillancourt, J.P.;Din, C.K.;Gallant, M.;Gareau, Y.;Griffin, P.R.;Labelle, M.;Lazebnik, Y.A.;Munday, N.A.;Raju, S.M.;Smulson, M.E.;Yamin, T.T.;Yu, V.L.;Miller, D.K.
  52. J. Cell Biol. v.123 Lazebnik, Y.A.;Cole, S.;Cooke, C.A.;Nelson, W.G.;Earnshaw, W.C.
  53. Nature (London) v.371 Lazebnik, Y.A.;Kaufmann, S.H.;Denoyers, S.;Poirier, G.G.;Earnshaw, W.C.