This study was conducted to obtain fundamental information on forage sorghum breeding in forage crop field of Livestock Experiment Station at Suwon from 1986 to 1991. The charcterization of sorghum germplasm was performed through 1986 to 1987, and after parental lines were selected from diverse sorghum germplasm on the basis of flowering date, plant height and several morphological characters for forage sorghum Fl hybrids. The range of variation of 50% flowering date and plant height were greater in order of forage sorghum sudangrass and male sterile line of grain sorghum. The average flowering date was earlier in sudangrass and male sterile line of grain sorghum than forage sorghum lines from the tested sorghum germplasms. And the average plant height was tall in order of forage sorghum, sudangrass and male sterile lines of grain sorghum. There were remarkable morphological variations between sudangrass lines and male sterile lines of grain sorghum such as plant color, leaf midrib color, glume color, seed coat color, head compactness and shape, awns, grain covering and 100 seed weight.
Soybean is the most promising crop for substituting rice on the paddy field. Excessive water stress is a common limiting factor in soybean yield under paddy soil condition. This study was carried out to identify changes in leaf total nitrogen and petiole ureide content under excess water conditions for establishing a screening system related to waterlogging tolerance. Waterlogging treatment was conducted by maintaining the water level on the soil surface for 10 days at the early vegetative growth stage ($V_5$) and the flowering stage ($R_2$). Leaf total nitrogen content, SPAD value and ureide content in petiole decreased in all soybean varieties in response to waterlogging, but the degree of decrease was much lesser in Pungsannamulkong and Muhankong than in Jangyeobkong and Myungjunamulkong, at 21 days after waterlogging treatment. This result means that root and nodule recovery rates were much higher in Pungsannamulkong and Muhankong than in Jangyeobkong and Myungjunamulkong after waterlogging treatment. The ureide and leaf nitrogen content showed high positive correlation with SPAD value, regardless of waterlogged stages. In conclusion, leaf nitrogen content, ureide content in petiole and leaf greenness were identified as promising indicator for screening soybeans which are tolerant of excess water.
Global climatic change and increasing climatic instability threaten crop productivity. Due to climatic change, drought stress is occurring more frequently in crop fields. In this study, we investigated the effect of treatment with hydrogen peroxide (H2O2) before leaf development on the growth and yield of sorghum for minimizing the damage of crops to drought. To assess the effect of H2O2 on the growth of sorghum plant, 10 mM H2O2 was used to treat sorghum leaves at the 3-leaf stage during growth in field conditions. Plant height, stem diameter, leaf length, and leaf width were increased by 7.6%, 9.6%, 8.3% and 11.5%, respectively. SPAD value, chlorophyll fluorescence (Fv/Fm), photosynthetic rate, stomatal conductance, and transpiration rate were increased by 3.0%, 4.9%, 26.0%, 23.4% and 12.7%, respectively. The amount of H2O2 in the leaf tissue of sorghum plant treated with 10 mM H2O2 was 0.7% of the applied amount after 1 hour. The level increased to approximately 1.0% after 6 hours. The highest antioxidant activity measured by the Oxygen Radical Absorbance Capacity assay was 847.3 µmol·g-1 at 6 hour after treatment. However, in the well-watered condition, the concentration of H2O2 in the plant treated by the foliar application of H2O2 was 227.8 µmol·g-1 higher than that of the untreated control. H2O2 treatment improved all the yield components and yield-related factors. Panicle length, plant dry weight, panicle weight, seed weight per plant, seed weight per unit area, and thousand seed weight were increased by 8.8%, 18.0%, 24.4%, 24.7%, 29.9% and 7.1%, respectively. Proteomic analysis showed that H2O2 treatment in sorghum increased the tolerance to drought stress and maintained growth and yield by ameliorating oxidative stress.
Oh, Se Jin;Yun, Hyun Soo;Oh, Seung Min;Kim, Sung Chul;Kim, Rog Young;Seo, Yung Ho;Lee, Kee Suk;Ok, Yong Sik;Yang, Jae E.
Journal of Applied Biological Chemistry
/
제56권4호
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pp.229-234
/
2013
Coal ash can be added to agricultural soils to increase the chemical properties of soil such as pH, cation exchange capacity and nutrient availability of - B, Ca, Mo etc-. Therefore, the main purpose of this study was to evaluate the feasibility of fly ash as a soil amendment in paddy soils. Selected fly ash was mixed with bentonite and calcium hydroxide at the ratio of 80:15:5 (w/w) and manufactured as a pellet type at the size of 10 mm. Field experiments were conducted to evaluate the effects of fly ash fertilizer on the soil quality and crop growth compare to the control (no fertilizer) and, - traditional fertilizer. Results showed that soil pH and organic matter in paddy soils after applying the manufactured fly ash fertilizer were not increased compared to the other two treatments. However, the concentration of available phosphate and silicate in paddy soils were higher than those of the control and traditional fertilization. With regard to crop growth, no significant difference was observed between three different treatments. However, the content of protein in the rice grain cultivated with the fly ash fertilizer was higher than in the rice cultivated by other two treatments. Overall, fly ash fertilizer could increase the concentration of available silicate and phosphate in the paddy soil and improve the rice quality. In conclusion, fly ash can be utilized in agricultural soils as soil amendment, especially in the rice paddy soil.
This survey was conducted to investigate the status of repair and maintenance of 4 wheel tractor for a basic reference to the improvement of quality and proper utilization of tractors. Thirty two counties from eight provinces, except Jeju, were covered in this study in order to investigate annual break-down and repair of tractor in 1980. The analyzed results are summarized as follows; 1. The average number of break-down of large size tractors(47ps) was 5.0 times in a year and it was about twice of that of small size tractors(19-23ps). The break-down frequency per 100 hours of use was 1.11 times in the large size and 0.65 times in the small size tractors. 2. 75.6 percent of total break-down was occured in main body of tractor and 24.4 percent in attachments. In particular, the break-down of plow and rotavator was more than 80 percent of total break-down of the small size tractor attachments. 3. The large size tractors which were occured more than one times of break-down a year was 75 percent and its rate of the small size tractor was 62 percent. But 9 percent of tractor surveyed had more than ten times of break-down in a year 4. The frequency of break-down had a peak in May, and it was directly proportional to the hours of use. 5. The causes of break-down were poor maintenance and operation by 29.8 precent, old parts by 30.2 percent, poor quality of parts by 20.6 percent, poor field condition by 16.3 percent and others by 3.1 percent. 6. Annual number of repair was 5.5 times and among them 55.6 percent was done by shop and 44.4 percent by operator. 7. Total required repair time was 30.6 hours a year in the large size tractor and 19.9 hours in the small size tractor. Average repair time was 3.62 hours a time. 8. Annual repair cost was 278 thousand won in the large size tractor and 70 thousand won in the small size tractor. The repair cost per hour of use was 621 won in the large size and 198 won in the small size tractor. 9. The repair cost rate of tractor(Y) was regulated with tractor age (X) as follow; Y=0.752X In case of the service life of tractor was 10 years, the total repair cost rate was 64 percent.
Soil samples collected from paddy field adjacent to zinc-mining sites were sequentially extracted to assess chemical fractions of Cd, Zn, Cu and Pb. The purpose of this study was two fold; (i) to examine the chemical forms of heavy metal in soils by sequential extraction. and (ii) to determine relationships between the chemical distribution of heavy metal in the soil and the heavy metal content of the brown rice. The results are summarized as follows. The content of exchangeable, organically bound and carbonate Cd and residual Zn was 73.9% and 63.8% of total Cd and Zn in the soil, respectively. The content of exchangeable Cd, Zn and Pb in soil showed highly negative correlations with pH, organically bound Cd, carbonate Cd, sulfide Cd, Zn and Pb in soil showed highly positive correlations with pH. The content of organically bound Cd, Zn, Pb and carbonate Cu in soil showed highly positive correlations with organic matter content, while the content of sulfide Cu and residual Cd in soil showed highly negative correlation with organic matter content. The content of carbonate Cd, Zn, Pb and residual Cu in soil showed highly positive correlations with CEC, but the content of exchangeable Cd, Zn, Cu, Pb and organically bound Cu in soil showed highly negative correlations with CEC. The content of total, organically bound, carbonate, sulfide and residual Cd in soil were highly correlated with that of Cd in brown rice. The content of any Pb fractions in soil were not correlated with that of Pb in brown rice. The content of water soluble and exchangeable Zn in soil were highly correlated with that of Zn in brown rice.
Green house soils have been intensively cultivated with excessive application of compost and chemical fertilizer for vegetable growth. The objective of this study was to establish the reasonable fertilizer application system for rice cultivation in green house soil. Field experiment was carried out with rice cv. Geumo-byeo 1 in Jisan series soil (fine loamy, mixed, mesic family of Fluventic Haplaquepts) that was previously cropped with green pepper (Capsicum annuum L.) for the last 3 years. Treatment consisted of conventional fertilization $(N-P_2O_5-K_2O=11-4.5-5.7kg\;10a^{-1})$, no basal fertilization, 50% reduction of basal fertilization no top dressing, bulk blending fertilizer, and no fertilizer. The value of pH, available phosphate, and exchangeable potassium after experiment was lower than those before experiment while organic matter content was not difference in all treatment. The value of salt elusion was the highest in no basal fertilization plot. The amount of $NH_4-N$ in soil was higher in growth stage of rice as fertilizer amount increased in 1998. The changes of plant height and tiller were higher as fertilizer amount increased. Thousand-grain weight as yield component was higher in no basal fertilization plot all the year because of decreasing panicle. There was no significant difference in rice yield between treatments in 1998. However, conventional fertilization resulted in significantly increased rice yield in 1999. Nitrogen use efficiency was the highest in no basal fertilization plot in 1998 and in conventional fertilization plot in 1998. Our results suggest that no basal fertilization be best to increase salt elusion with slightly increased yield in first year for rice cropping after vegetable harvesting, which method improves fertilization efficiency. However, conventional fertilization was good for second rice cropping after vegetable harvesting in greenhouse.
Kim, Sung Un;Kim, Yong Gyun;Lee, Sang Mong;Park, Hyean Cheal;Kim, Keun Ki;Son, Hong Joo;Yun, Sung Wook;Kim, Sang Yoon;Hong, Chang Oh
Korean Journal of Environmental Agriculture
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제35권2호
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pp.152-157
/
2016
BACKGROUND: Since bottom ash (BA) contains considerable amounts of CaO and MgO, it could be a useful amendment to increase soil pH and to immobilize cadmium (Cd). This study was conducted to evaluate effect of BA application in reducing Cd phytoavailability.METHODS AND RESULTS: Bottom ash was applied at the rate of 0, 20, 40, and 80 Mg/ha to Cd contaminated soil, and then lettuce was cultivated under field condition. soil pH and net negative charge increased slightly with increasing BA application; however, there was no statistical difference among the rates. Water soluble, exchangeable+acidic, reducible, and oxidizable fraction of Cd decreased with increasing bottom ash application rate, whereas residual fraction of Cd increased with increasing bottom ash application rate. Lettuce yield increased with rate of bottom ash up to 40 kg/ha. Visual evidences of cadmium toxicity and growth inhibition were not found during lettuce cultivation.CONCLUSION: Bottom ash was effective to reduce phytoextractability of Cd and to increase lettuce yield. Conclusively, BA could be a good soil amendment to reduce Cd phytoavailability in contaminated arable soil.
Na-Ca exchange transports calcium ion either into (reverse mode Na-Ca exchange) or out of the cell (forward mode Na-Ca exchange) according to the direction of driving force produced by the changes in ratio of intra- and extra-cellular Na concentrations. Thus, Na-Ca exchange is regarded as the regulator of myocardial contraction. However, the existence of reverse mode Na-Ca exchange and its role in myocardial contraction is still questioned. Present study was performed to identify the presence of reverse mode Na-Ca exchange and its possible involvement in the regulation of myocardial contraction in rat heart. Using the left atria of rat, contraction was induced by electrical field stimulation (EFS, 0.5 msec duration and supramaximal voltage). Changing of the stimulation frequencies from resting 4 Hz to 0.4, 1 or 8 Hz caused typical negative staircase effect in twitch tension, but $^{45}Ca$ uptake showed bimodal increase. When the stimulation frequency was abruptly changed from 4 Hz to 0.4 Hz the atrial twitch tension showed three phased-enhancement, that is, the initial rapid increase (the first phase) followed by rapid decrease (the second phase) and stabilization (the third phase). $^{45}Ca$ uptake was equivalent to tension, i.e. initial significant increase in first 30 second and then decrease. Benzamil treatment abolished the first phase of increase in a dose dependent manner from $10^{-5}\;to\;3{\times}10^{-4}M.$ Bay k 8644 $(3{\times}10^{-5}M)$ treatment enhanced the inotropy induced by frequency reduction and abolished the second and third phase decreases. Benzamil treatment also suppressed the contraction stimulated by Bay K 8644. Although the contraction at 4 Hz stimulation was completely abolished by verapamil $3{\times}10^{-5}\;M$ pretreatment, the contraction reappeared as soon as the stimulation frequency was changed into 0.4 or 1 Hz and interstingly,$^{45}Ca$ uptake were significantly higher than no treatment. From these results, it is concluded that reduction of stimulation frequency causes calcium influx by the reverse mode Na-Ca exchange, resulting in initial rapid increase of twitch tension. then it turns into forward mode exchange to efflux the calcium, resulting in decrease of the twitch tension in left atria of rat.
Park, Kyeong Hun;Park, Hong Woo;Lee, Seong Woo;Lee, Seung Ho;Myung, Kyung Sun;Lee, Sang Yeob;Song, Jaekyeong;Kim, Young Tak;Park, Kyoung Soo;Kim, Young Ock
The Korean Journal of Pesticide Science
/
제20권4호
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pp.380-387
/
2016
This study was performed to select potentially available biological control agent from soil bacteria for prevention of ginseng damping off. More than five hundred strains were isolated from ginseng rhizosphere soil. By testing antifungal activity, we have selected three soil bacteria strains and their ability to produce antibiotics and lytic enzymes such as cellulase, protease and pectate lyase was examined. Also, the presence of genes for biosynthesis of lipopeptide such as fengycin, bacillomycin D, surfactin, iturin A, and zwittermicin A was investigated in selected strains. All three strains produced cellulase, protease, and xylanase. Moreover, these strains had gene for biosynthesis of bacillomycin D, surfactin, and iturin A. ES1 and ES3 strains were identified Bacillus methylotrophucus and ES2 was confirmed Bacillus amyloliquefaciens using phylogenetic analysis on the basis of 16S rRNA gene sequences. In field test, control value of ES1, ES2 and ES3 treatment was 32.4%, 46.8% and 36.7%, respectively. This results indicate that antagonistic microbes with high ability of antifungal and lytic enzyme activity can be used as a useful biological control agent to control ginseng damping off.
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