The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Isolation and Characterization of Bacillus Species Having Antifungal Activity Against Pathogens of Ginseng Damping Off

인삼모잘록병원균에 항균활성을 갖는 Bacillus 균의 분리 및 특성조사

  • Park, Kyeong Hun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Park, Hong Woo (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Seong Woo (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Seung Ho (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Myung, Kyung Sun (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA) ;
  • Lee, Sang Yeob (Agricultural microbiology division, National Institute of Agricultural Science, RDA) ;
  • Song, Jaekyeong (Agricultural microbiology division, National Institute of Agricultural Science, RDA) ;
  • Kim, Young Tak (Seed Certification Team, Quality Assurance Division, Nongwoo Bio CO., LTD.) ;
  • Park, Kyoung Soo (Seed Certification Team, Quality Assurance Division, Nongwoo Bio CO., LTD.) ;
  • Kim, Young Ock (Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA)
  • 박경훈 (국립원예특작과학원 인삼특작부) ;
  • 박홍우 (국립원예특작과학원 인삼특작부) ;
  • 이성우 (국립원예특작과학원 인삼특작부) ;
  • 이승호 (국립원예특작과학원 인삼특작부) ;
  • 명경선 (국립원예특작과학원 인삼특작부) ;
  • 이상엽 (국립농업과학원 농업미생물과) ;
  • 송재경 (국립농업과학원 농업미생물과) ;
  • 김영탁 (농우바이오 종자검정팀) ;
  • 박경수 (농우바이오 종자검정팀) ;
  • 김영옥 (국립원예특작과학원 인삼특작부)
  • Received : 2016.10.24
  • Accepted : 2016.11.23
  • Published : 2016.12.31
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Abstract

This study was performed to select potentially available biological control agent from soil bacteria for prevention of ginseng damping off. More than five hundred strains were isolated from ginseng rhizosphere soil. By testing antifungal activity, we have selected three soil bacteria strains and their ability to produce antibiotics and lytic enzymes such as cellulase, protease and pectate lyase was examined. Also, the presence of genes for biosynthesis of lipopeptide such as fengycin, bacillomycin D, surfactin, iturin A, and zwittermicin A was investigated in selected strains. All three strains produced cellulase, protease, and xylanase. Moreover, these strains had gene for biosynthesis of bacillomycin D, surfactin, and iturin A. ES1 and ES3 strains were identified Bacillus methylotrophucus and ES2 was confirmed Bacillus amyloliquefaciens using phylogenetic analysis on the basis of 16S rRNA gene sequences. In field test, control value of ES1, ES2 and ES3 treatment was 32.4%, 46.8% and 36.7%, respectively. This results indicate that antagonistic microbes with high ability of antifungal and lytic enzyme activity can be used as a useful biological control agent to control ginseng damping off.

인삼 모잘록병을 일으키는 병원균의 생물적 방제제 개발을 위하여 인삼 근권 토양으로 부터 500종의 미생물을 분리하였다. 인삼모잘록병원균을 대상으로 항균활성을 검정한 결과, 항균활성이 우수한 균주 3종을 선발하였다. 선발한 균주를 대상으로 항생물질과 세포벽분해효소 생성능력을 조사하였으며, fengycin, bacillomycin D, surfactin, iturin A와 zwittermicin A와 같은 리포펩타이드 생합성 유전자 유무를 조사하였다. ES1과 ES3 균주에서 iturin A와 surfactin 생합성 유전자를 확인하였으며, 세포벽 분해효소 생성능을 확인한 결과 선발한 모든 균주에서 cellulase, pectate lyase, protease를 생성하였다. 16S rRNA 염기서열 분석에 기반하여 계통도를 분석한 결과 ES1 균주와 ES3 균주는 Bacillus methylotrophucus로 확인되었으며, ES2는 B. amyloliquefaciens로 동정되었다. 포장실험 결과 ES1, ES2, ES3 처리구에서 각각 32.4%, 46.8%, 36.7%의 방제효과를 보였다. 이러한 결과로부터 항균활성과 세포벽 분해 효소 생성능이 우수한 길항균주를 선발하였으며, 향후 포장실험과 제형화 개발 등을 통해 인삼모잘록병 방제를 위한 생물적 방제제로 활용할 수 있을 것으로 기대된다.

Keywords

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