• Korean Journal of Clinical Laboratory Science
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• v.38 no.2
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• pp.135-140
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• 2006

Magnetocardiography (MCG) is the measurement and analysis of the magnetic component of the electro-magnetic field of the human heart, usually conducted externally, using extremely sensitive devices such as a Superconducting Quantum Interference Device (SQUID). MCG is a totally noninvasive method, it uses neither radiation nor ultrasonics. The magnetic activity of the heart is registered from outside the thorax. MCG has a very high sensitivity and a high spatial resolution for very a small, local myocardial current. In comparison to the electrical signals measured by an ECG, the magnetic signal does not disturb the boundaries of tissues with different electrical properties. MCG measures the myocardial function rather than describing the morphology. MCG is a relatively new technique that promises good spatial resolution and extremely high temporal resolution, thus complementing other heart activity measurement techniques such as Electrocardiography (ECG). The clinical uses of MCG are in detecting various cardiac disorders including myocardial infarction, ventricular hypertrophy, ventricular conduction defects, Wolff-Parkinson-White (WPW) syndrome, sudden cardiac death and fetal magnetocardiography. Magnetocardiography may be used alone or together with electrcardiography for the measurement of spontaneous or overloaded activity and for research or clinical purposes.

• Advances in Traditional Medicine
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• v.6 no.3
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• pp.245-251
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• 2006

We examined the radioprotection effects of acupoint (acupuncture point) stimulation during organogenesis stages of ICR mice. Pregnant mice received 1.5 Gy whole body X-irradiation on day 8 of gestation, which is the early stage of organogenesis. The embryonic death rate and teratogenesis rate by radiation were examined. Electroacupuncture to the leg acupoints and/ or transcutaneous stimulation to the back acupoints on the pregnant mice showed no protective effect against irradiation on embryonic or fetal death rate. On the contrary, the strong stimulation resulted in increase in the mortality after irradiation rather than protection. However acupoint stimulation to the pregnant mice never showed harmful effects by itself on embryos. It tended to reduce the skeletal malformations induced by X-ray irradiation. We suspect that acupoint stimulation removed the cells injured by irradiation during embryonic development, resulting in an increase in embryonic death rate and reduction in skeletal anomalies.

• Clinical and Experimental Reproductive Medicine
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• v.39 no.1
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• pp.1-9
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• 2012

The safety of human exposure to an ever-increasing number and diversity of electromagnetic field (EMF) sources both at work and at home has become a public health issue. To date, many in vivo and in vitro studies have revealed that EMF exposure can alter cellular homeostasis, endocrine function, reproductive function, and fetal development in animal systems. Reproductive parameters reported to be altered by EMF exposure include male germ cell death, the estrous cycle, reproductive endocrine hormones, reproductive organ weights, sperm motility, early embryonic development, and pregnancy success. At the cellular level, an increase in free radicals and $[Ca^{2+}]i$ may mediate the effect of EMFs and lead to cell growth inhibition, protein misfolding, and DNA breaks. The effect of EMF exposure on reproductive function differs according to frequency and wave, strength (energy), and duration of exposure. In the present review, the effects of EMFs on reproductive function are summarized according to the types of EMF, wave type, strength, and duration of exposure at cellular and organism levels.

• Journal of Yeungnam Medical Science
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• v.21 no.2
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• pp.251-255
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• 2004

Spontaneous uterine rupture of the unscarred uterus during the second trimester of pregnancy is rare, but it is a surgical emergency. Because it results in rapid deterioration of patient and high mortality despite of prompt operation and massive transfusion, early diagnosis and proper management are critical for optimizing patient care. We present a case of spontaneous uterine rupture with fetal death in 14 weeks gestation with a brief review of literatures.

• Proceedings of the Korean Society of Embryo Transfer Conference
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• 2002.11a
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• pp.43-55
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• 2002

Cloning by nuclear transfer in mammals using somatic cells has enormous potential applications. However, somatic cloning has been inefficient in all species in which NT is successful. High abortion and fetal death rates have been observed. These developmental defects have been attributed to incomplete nuclear reprogramming by the somatic cloning process. In this review, we will discuss studies conducted in our labs to understand the nuclear reprogramming process.

• Journal of radiological science and technology
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• v.37 no.1
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• pp.21-28
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• 2014

Cultured pancreatic beta cells and nerve cells, it is given normal condition of 10% FBS (fetal bovine serum), 11.1 mM glucose and hyperglycemia codition of 1% FBS, 30 mM glucose. For low LET X-ray irradiated with 0.5 Gy/hr dose-rate(total dose: 0.5 to 5 Gy). Survival rates were measured by MTT assay. When non irradiated, differentiated in the pancreatic beta cells experiment is hyperglycemia conditions survival rate compared to normal conditions survival rate seemed a small reduction. However increasing the total dose of X-ray, the survival rate of normal conditions decreased slightly compared to the survival rate of hyperglycemia conditions, the synergistic effect was drastically reduced. When non irradiated, undifferentiated in the nerve cells experiment is hyperglycemia conditions survival rate compared to normal conditions survival rate seemed a large reduction. As the cumulative dose of X-ray normal conditions and hyperglycemia were all relatively rapid cell death. But the rate of decreased survivals by almost parallel to the reduction proceed and it didn't show synergistic effect.

• Development and Reproduction
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• v.5 no.2
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• pp.93-100
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• 2001

Precise mechanism by which the fetus can escape from mother's immune rejection is not well understood yet over the last 50 years. The clarification of immune mechanism at the feto-maternal interface is very important, because this can be a common pathogenesis of various pathologic conditions including spontaneous abortion, habitual abortion fetal growth restriction preeclampsia, implantation failure after assisted reproductive techniques, and fetal death. In this review, current hypothetical contents were described with the priority of importance: 1) The center of this mechanism is cross-talk between the expression of HLA-C, E, G on the extravillous cytotrophoblasts and their receptors on decidual NK cell, 2) immunomodulation, 3) innate immunity is the main immunologic mechanism, 4) various mechanisms besides HLA system(eq. complement) may be associated. The overall balance of immunomodulation among these mechanisms should result in the outcome of each pregnancy. Further researches regarding the regulation of HLA system, roles of cytokines, complements should be followed in the future.

• Clinical and Experimental Pediatrics
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• v.50 no.3
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• pp.306-310
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• 2007

Restrictive dermopathy is a rare autosomal recessive disorder in which rigidity or tautness of the skin from the second trimester causes a fetal akinesia deformation sequence (FADS) and early death. Characteristic features include taut skin with prominent subcutaneous vessels, widely open fontanelles and cranial sutures, distinctive facies, flexion contractures, pulmonary hypoplasia, sparse eyelashes and and eyebrows, thin dysplastic clavicles. The histologic abnormalities of the skin are located in a thin dermis, consisting of compactly arranged collagen fibers, scanty elastic fiber. The dermoepidermal junction is flat, and epidermal appendages are absent, miniaturized or immature. The presence of adipose tissue may be increased. We report on the first Korean case of restrictive dermopathy with typical clinical features and histological findings.

• Development and Reproduction
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• v.2 no.1
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• pp.1-8
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• 1998

In mammalian ovary, major portion(>99%) of ovarian follicles undergo atresia. Recent studies have shown that this phenomenon is mediated via GC apoptosis. Ceramide, a product of sphingomyelin hydrolysis, has been proposed as a novel lipid second messenger with specific roles in mediating antiproliferative responses including apoptosis and cell cycle arrest. In the present study, we have examined the effect of ceramide on apoptotic cell death of GC in vitro. GCs were harvested by squeezing the antral follicles from the immature mice (3-4 weeks) and cultured in MEM medium with 10% fetal bovine serum. The cells were treated with various concentrations of ceramide (0 to 50 \mu M)and cultured up to 24 h.Cell death was determined by MTT cell viability assay and apoptosis was examined by acridine orange staining, in situ 3'-end labeling(TUNEL), and flow cytometry. Ceramid treatment induced apoptotic cell death of GC in a time- and a dose-dependent manner. Results of flow cytometric analysis showed that creamide-induced cell death was mostly confined to the $G_{0}$/$G_{1}$ cells. these results provide an evidence for ceramide as a lipid second messenger of apoptosis in mouse GC.

• Women's Health Nursing
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• v.1 no.2
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• pp.174-199
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• 1995

This study examined the antenatal care known as Taekyo. The sample consisted of 795 women and 564 men who had be seen in the antenatal care unit, delivery room, or postpartal care unit of general hospitals in Korea between March 20 and April 22, 1995. Data were collected using the "Taekyo questionnaire for childbearing women" and "Taekyo questionnaire for husbands of childbearing women" developed by researchers. Data were self-reported. Data were analyzed using the SPSS PC+ program, and descriptive statistics, T-test, ANOVA, Pearson correlation coefficient were applied. The results of this study were as follows : 1. Encouragement to practice Taekyo was at a relatively high level. Items on Taekyo encouraging behaviors had a mean score of 3.51(33 items 5 point scale). According to the five categories of Taekyo encouraging behaviors, subjects indicated they practice "food intake(mean score 4.02)". "praying(mean score 3.78)", "cumulative virtuous deeds (mean score 3.58)". "mind and body management(mean score 3.47)", "maternal fetal interaction(mean score 3.15)". 2. The childbearing women's practices related to forbidden behaviors by Taekyo were relatively high. Item on forbidden behaviors by Taekyo had a mean score 3.71(43 items 5 point scale). According to the five categories of forbidden behaviors by Taekyo, subjects indicated they practiced "voluntary abstention of drugs(mean score 4.78)," avoiding behaviors(mean score 4.78)," avoiding behaviors(mean score 3.77), "good behaviors(mean score 3.71)", "taboo on fetal death tendency (mean score 3.53)", "taboo on certain intake(mean score 3.47)". 3. The practice score of husbands for childbearing women which related to Taekyo behaviors were relatively high. Item on Taekyo behaviors had a mean score 3.59(33 items 5 point scale). According to the six categories of Taekyo behavior, subjects indicated they practiced "mind and body management(mean score 3.94)", "praying(mean score 3.80)", "support of women’s practice related to Taekyo(mean score 3.66)", "good behaviors(mean score 3.58)", "continency(mean score 3.33)", "paternal fetal interaction (mean score 3.19)". 4. On the childbearing women's perception of Taekyo, most of the subjects(88%) had confidence in the positive effects of Taekyo on Child development. The result showed that the childbearing couples practiced Taekyo behavior relatively often and sincerely and most of the childbearing women had a positive perception of Taekyo's effect on prenatal child development.