• Microbiology and Biotechnology Letters
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• v.14 no.5
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• pp.365-369
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• 1986

The activity of glucoamylase and pullulanase, properties of glucoamylase and ethanol productivities of fusants were studied. Glucoamylase and pullulanase activity of fusants were higher than parents. The optimal pH and temperature of glucoamylase of fusants were very similar to the those produced by S. diastaticus. In alcohol fermentation. fermenting ability and fermentation rate of fusants were higher and faster than either of its parental strain. The maximum of alcohol yield in 15% of liquefied potato starch was 7.8% (v/v)

• Journal of Microbiology and Biotechnology
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• v.3 no.3
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• pp.168-173
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• 1993

The expression kinetics of human lipocortin I (LCI), a potential anti-inflammatory agent, was studied in the shake-flask and fermenter cultures of Saccharomyces cerevisiae carrying a galactose-inducible expression system. The cell growth, expression level of LCI, and the plasmid stability were investigted under various galactose induction conditions. The expression of LCI was repressed by the presence of a very small amount of dextrose in the culture medium, but it was induced by galactose after dextrose became completely depleted. The optimal ratio of dextrose to galactose for lipocortin I production was found to be 1.0 (10 g/l dextrose and 10 g/l galactose). With optimal D/G ratio of 1.0 and the addition of galactose prior to dextrose depletion, LCI of about 100~130 mg/l was produced. LCI at a concentration of 174 mg/l was porduced in the fed-batch culture, which was nearly a twice as much of that produced in the batch culture. The plasmid stability was very high in all culture cases, and thus was considered to be not an important parameter in the expression of LCI.

• Journal of Microbiology and Biotechnology
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• v.10 no.2
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• pp.195-200
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• 2000

Bacillus cereus BS229 was identified as a bacteriocin producer with a bactericidal activity against Bacillus thuringiensis subsp. Thomsoni BR-40. Bacillus cereus BS229 and cerein BS229, named tentatively as the bacteriocin produced by Bacillus cereus BS229, showed a narrow spectrum of actibity against Gram-positive and Gram-negative bacteria, along with yeast and molds. Production of cerein BS229 in a 5-1 fermenter followed typical kinetics of primary metabolite synthesis. The antibacterial activity of cerein BS229 on sensitive indicator cells disappeared completely by ${\alpha}-chmotrypsin$ or proteinase K, which indicates its proteinaceous nature. Cerein BS229 seemed to be very stable throughout the pH range of 2.0 of 9.0 and it was relatively heat labile, despite the fact that bacteriocin activity was still detected after being boied for 30min. Cerein BS229 actibity has been changed with some of the organic solvents such as toluene, ethanol, and chloroform. Direct detection of cerein BS229 actibity on SDS-PAGE suggested that it had an apparent molecular mass of about 8.2 kDa.

• Mycobiology
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• v.42 no.2
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• pp.193-197
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• 2014

Laccase activity of Pleurotus ostreatus is significantly increased by the addition of apple pomace. Among various conditions, the best concentration of apple pomace and cultivation time for the production of laccase by P. ostreatus was 2.5% and 9 days, respectively. Reverse transcription polymerase chain reaction analyses of laccase isoenzyme genes, including pox1, pox3, pox4, poxc, poxa3, and poxa1b, revealed a clear effect of apple pomace on transcription induction. Our findings reveal that the use of apple pomace can be a model for the valuable addition of similar wastes and for the development of a solid-state fermenter and commercial production of oyster mushroom P. ostreatus.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.287-290
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• 2000

To examine effects of agitation and aeration as well as adding of glucose and yeast extract on cell growth and polysaccharide production by Paecilomyces japonica, batch culture was carried out at 5L jar fermenter at $27^{\circ}C$ with the initial pH 7 for 7 days cultivation(innoculum size 2%, working volume 3L). Media compositions(g/L) were 30 glucose, 20 yeast extract, 0.5 $KH_2PO_4$, $0.1\;CuCl_2\;{\cdot}\;2H_2O$. Optimum culture conditions of agitation and aeration in batch culture were 400 rpm and 1.0 vvm, resulting in 23.1 g/L biomass and 2.5 g/L polysaccharide. Additional feeding of glucose and yeast extract with a pulse mode conferred an advantage on cell growth and polysaccharide production with showing the results of 29.2 g/L and 3.3 g/L, respectively.

• 한국생물공학회:학술대회논문집
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• 2000.11a
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• pp.237-240
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• 2000

Organic acids which were produced from biomass wastes streams by cell-recycle fermentation using Propionibacterium acidipropionici ATCC 4965 were extracted by Membrane Contactor using TOA/MIBK system. Maximum productivity was 3.32g organic acid/L/hr at the dilution rate of 0.2/hr in the results of continuous fermentation. The diluted organic acids in the fermenter were selectively separated by Membrane Contactor extraction using 30%(w/w) trioctylamine(TOA) dissolved in methylisobutylketone(MIBK). The flow rate of aqueous phase is 200ml/min and that of extraction phase is 100ml/min. The degree of Acetic acid and Propionic acid extraction from fermentation broth was reached 56.25%, 72.41% respectively.

• Journal of Marine Bioscience and Biotechnology
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• v.10 no.1
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• pp.18-25
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• 2018

Chlorella is quantitatively and qualitatively high in protein with balanced essential amino acid profiles, vitamins and minerals. ${\gamma}-Aminobutyric$ acid (GABA) is broadly distributed in nature and fulfills multi-physiological functions including effect such as a health-promoting functional compound. To improve the GABA production, Chlorella protothecoides were grown through the modified mixtrophic culture medium containing 2L of sterilized bristol medium with 0.01% urea and 4.0% glucose in a 5L fermenter. The results showed that nineteen kinds of amino acid including GABA at C. protothecoides sample were analyzed using high performance liquid chromatography (HPLC). Glutamic acid in total concentration (%) of amino acid is the most abundant amino acid (33.10%), followed by alanine (20.48%) and GABA (17.48%). Three amino acids including GABA were responsible for more than 70% total concentration in C. protothecoides sample including eight essential and nine non-essential amino acids: aspartic acid, asparagine, serine, glutamine, histidine, glycine, threonine, arginine, tyrosine, valine, methionine, tryptophan, phenylalanine, isoleucine, leucine, lysine. As a result of this experiment, it is expected that Chlorella will be developed to a critical product having high value as, GABA, functional food materials.

• Journal of Ginseng Research
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• v.40 no.2
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• pp.121-126
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• 2016

Background: Ginsenoside F1, a pharmaceutical component of ginseng, is known to have antiaging, antioxidant, anticancer, and keratinocyte protective effects. However, the usage of ginsenoside F1 is restricted owing to the small amount found in Korean ginseng. Methods: To enhance the production of ginsenoside F1 as a 10 g unit with high specificity, yield, and purity, an enzymatic bioconversion method was developed to adopt the commercial enzyme Cellulase KN from Aspergillus niger with food grade, which has ginsenoside-transforming ability. The proposed optimum reaction conditions of Cellulase KN were pH 5.0 and $50^{\circ}C$. Results: Cellulase KN could effectively transform the ginsenosides Re and Rg1 into F1. A scaled-up biotransformation reaction was performed in a 10 L jar fermenter at pH 5.0 and $50^{\circ}C$ for 48 h with protopanaxatriol-type ginsenoside mixture (at a concentration of 10 mg/mL) from ginseng roots. Finally, 13.0 g of F1 was produced from 50 g of protopanaxatriol-type ginsenoside mixture with $91.5{\pm}1.1%$ chromatographic purity. Conclusion: The results suggest that this enzymatic method could be exploited usefully for the preparation of ginsenoside F1 to be used in cosmetic, functional food, and pharmaceutical industries.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 1997.05a
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• pp.23-27
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• 1997

Bio-Green(B.G.) functional water was manufactured by Kyungwon Enterprise Co. through a series of processes ; water longrightarrow ultra-purification longrightarrow adding catalysts longrightarrow energy imprinting fermenting with energized water + zeolite and others + photosynthetic bacteria in fermenter longrightarrow filtering. Control(0), 5 or 10 liters of B.G. functional water were supplied to the orchard soil under canopy of 10 year old 'Tsugaru'/M26 apple trees on March 20, May 20 and June 20, 1995, respectively. Some orchard soil characteristics, not only pH, but also Ca and Mg of exchangeable cations were increased by supply with B.G. functional water. However, P$_2$O$_{5}$, K, and B contents were not influenced by the treatment. At harvest time soluble solid content of flesh and anthocyanin of fruit skin were increased by the treatment. B.G. functional water treatment showed higher root activities, and photosynthesis of leaves than that of control. Also B.G. functional water treatment showed higher Ca content in fruit skin and flesh tissues, whereas not affected N, K, and Mg contents. During storage at 4$^{\circ}C$ cold room, the more volume of B.G. functional water supply showed lower bitter pit symptom. Respiration and ethylene evolution in fruit were decreased, while fruit firmness increased by the treatment during storage.e.

• KSBB Journal
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• v.19 no.3
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• pp.226-230
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• 2004

Fermentation characteristics of D-xylose into xylitol by Candida mogii ATCC 18364, a potential xylitol producer from rice straw hemicellulose hydrolyzates, were investigated. The influences of the most important operational variables on xylitol production were examined. The best results in xylitol production were obtained in shake-flask fermentations when 3.0 g/L initial cell concentration of 12 hr-old cells grown in D-glucose containing medium were used as inoculum. The oxygen availability is a critical factor in xylose fermentation, therefore, xylose conversion into xylitol was investigated in a 2-L fermenter at different stirring rates. Maximum xylitol production was obtained with an aeration rate of 1 vvm at a stirring rate of 200 rpm.