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http://dx.doi.org/10.15433/ksmb.201810.1.018

The Content Analysis of Amino Acids Including GABA of Chlorella protothecoides under Mixtrophic Culture  

Jeong, Yu Jeong (Biois. Co., Ltd.)
Kim, Seong Hak (Biois. Co., Ltd.)
Min, Hee Gyung (Biois. Co., Ltd.)
Kim, Sung Chun (Biois. Co., Ltd.)
Publication Information
Journal of Marine Bioscience and Biotechnology / v.10, no.1, 2018 , pp. 18-25 More about this Journal
Abstract
Chlorella is quantitatively and qualitatively high in protein with balanced essential amino acid profiles, vitamins and minerals. ${\gamma}-Aminobutyric$ acid (GABA) is broadly distributed in nature and fulfills multi-physiological functions including effect such as a health-promoting functional compound. To improve the GABA production, Chlorella protothecoides were grown through the modified mixtrophic culture medium containing 2L of sterilized bristol medium with 0.01% urea and 4.0% glucose in a 5L fermenter. The results showed that nineteen kinds of amino acid including GABA at C. protothecoides sample were analyzed using high performance liquid chromatography (HPLC). Glutamic acid in total concentration (%) of amino acid is the most abundant amino acid (33.10%), followed by alanine (20.48%) and GABA (17.48%). Three amino acids including GABA were responsible for more than 70% total concentration in C. protothecoides sample including eight essential and nine non-essential amino acids: aspartic acid, asparagine, serine, glutamine, histidine, glycine, threonine, arginine, tyrosine, valine, methionine, tryptophan, phenylalanine, isoleucine, leucine, lysine. As a result of this experiment, it is expected that Chlorella will be developed to a critical product having high value as, GABA, functional food materials.
Keywords
Chlorella protothecoides; Mixotrophic culture; GABA; Amino acid;
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