• 제목/요약/키워드: feed analysis laboratory

검색결과 117건 처리시간 0.032초

Heterologous Expression of a Thermostable α-Galactosidase from Parageobacillus thermoglucosidasius Isolated from the Lignocellulolytic Microbial Consortium TMC7

  • Wang, Yi;Wang, Chen;Chen, Yonglun;Cui, MingYu;Wang, Qiong;Guo, Peng
    • Journal of Microbiology and Biotechnology
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    • 제32권6호
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    • pp.749-760
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    • 2022
  • α-Galactosidase is a debranching enzyme widely used in the food, feed, paper, and pharmaceuticals industries and plays an important role in hemicellulose degradation. Here, T26, an aerobic bacterial strain with thermostable α-galactosidase activity, was isolated from laboratory-preserved lignocellulolytic microbial consortium TMC7, and identified as Parageobacillus thermoglucosidasius. The α-galactosidase, called T26GAL and derived from the T26 culture supernatant, exhibited a maximum enzyme activity of 0.4976 IU/ml when cultured at 60℃ and 180 rpm for 2 days. Bioinformatics analysis revealed that the α-galactosidase T26GAL belongs to the GH36 family. Subsequently, the pET-26 vector was used for the heterologous expression of the T26 α-galactosidase gene in Escherichia coli BL21 (DE3). The optimum pH for α-galactosidase T26GAL was determined to be 8.0, while the optimum temperature was 60℃. In addition, T26GAL demonstrated a remarkable thermostability with more than 93% enzyme activity, even at a high temperature of 90℃. Furthermore, Ca2+ and Mg2+ promoted the activity of T26GAL while Zn2+ and Cu2+ inhibited it. The substrate specificity studies revealed that T26GAL efficiently degraded raffinose, stachyose, and guar gum, but not locust bean gum. This study thus facilitated the discovery of an effective heat-resistant α-galactosidase with potent industrial application. Meanwhile, as part of our research on lignocellulose degradation by a microbial consortium, the present work provides an important basis for encouraging further investigation into this enzyme complex.

Metabolic profiling of serum and urine in lactating dairy cows affected by subclinical ketosis using proton nuclear magnetic

  • Eom, Jun Sik;Lee, Shin Ja;Kim, Hyun Sang;Choi, Youyoung;Jo, Seong Uk;Lee, Sang Suk;Kim, Eun Tae;Lee, Sung Sill
    • Journal of Animal Science and Technology
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    • 제64권2호
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    • pp.247-261
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    • 2022
  • Ketosis is associated with high milk yield during lactating or insufficient feed intake in lactating dairy cows. However, few studies have been conducted on the metabolomics of ketosis in Korean lactating dairy cows. The present study aimed to investigate the serum and urine metabolites profiling of lactating dairy cows through proton nuclear magnetic resonance (1H-NMR) spectroscopy and comparing those between healthy (CON) and subclinical ketosis (SCK) groups. Six lactating dairy cows were categorized into CON and SCK groups. All experimental Holstein cows were fed total mixed ration. Serum and urine samples were collected from the jugular vein of the neck and by hand sweeping the perineum, respectively. The metabolites in the serum and urine were determined using 1H-NMR spectroscopy. Identification and quantification of metabolites was performed by Chenomx NMR Suite 8.4 software. Metabolites statistical analysis was performed by Metaboanalyst version 5.0 program. In the serum, the acetoacetate level was significantly (p < 0.05) higher in the SCK group than in the CON group, and whereas acetate, galactose and pyruvate levels tended to be higher. CON group had significantly (p < 0.05) higher levels of 5-aminolevulinate and betaine. Indole-3-acetate, theophylline, p-cresol, 3-hydroxymandelate, gentisate, N-acetylglucosamine, N-nitrosodimethylamine, xanthine and pyridoxine levels were significantly (p < 0.05) higher in the urine of the SCK group than that in the CON group, which had higher levels of homogentisate, ribose, gluconate, ethylene glycol, maltose, 3-methyl-2-oxovalerate and glycocholate. Some significantly (p < 0.05) different metabolites in the serum and urine were associated with ketosis diseases, inflammation, energy balance and body weight. This study will be contributed useful a future ketosis metabolomics studies in Korea.

Supplementation of guanidinoacetic acid and rumen-protected methionine increased growth performance and meat quality of Tan lambs

  • Zhang, Jian Hao;Li, Hai Hai;Zhang, Gui Jie;Zhang, Ying Hui;Liu, Bo;Huang, Shuai;Guyader, Jessie;Zhong, Rong Zhen
    • Animal Bioscience
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    • 제35권10호
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    • pp.1556-1565
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    • 2022
  • Objective: Tan lambs (n = 36, 3 mo old, 19.1±0.53 kg) were used to assess effects of dietary guanidinoacetic acid (GAA) and rumen-protected methionine (RPM) on growth performance, carcass traits, meat quality, and serum parameters. Methods: Lambs were randomly assigned to three treatment groups, with 6 pens per group and 2 lambs per pen. Dietary treatments were: basal diet alone (I); basal diet supplemented with 0.08% GAA+0.06% RPM (II); and basal diet supplemented with 0.08% GAA+0.08% RPM (III). Diets were provided three times a day for 90 d. Intake per pen was recorded daily and individual lamb body weight (BW) was measured monthly. Carcass traits were measured after slaughter and meat quality at the end of the experiment, blood samples were taken on a subgroup of lambs for analysis of indicators mostly related to protein metabolism. Results: Final BW and average daily gain for the first and second month, and for the entire experiment were greater in Treatment II compared to Treatment I (p<0.05), whereas feed to gain ratio was lower (p<0.05). Treatment II had the optimal dressing percentage and net meat weight proportion, as well as crude protein and intramuscular fat concentrations in muscles. Treatment II improved meat quality, as indicated by the greater water holding capacity, pH after 45 min and 48 h, and lower shear force and cooking loss. Dietary supplementation of GAA and RPM also increased the meat color a* and b* values at 24 h. Finally, Treatment II increased total protein, and serum concentrations of albumin and creatinine, but decreased serum urea nitrogen concentrations, indicating improved protein efficiency. Conclusion: In this study, 0.08% GAA+0.06% RPM supplementation improved growth performance and meat quality of Tan lambs.

Use of Leucaena leucocephala and Gliricidia sepium as Nitrogen Sources in Supplementary Concentrates for Dairy Goats Offered Rhodes Grass Hay

  • Ondiek, J.O.;Tuitoek, J.K.;Abdulrazak, S.A.;Bareeba, F.B.;Fujihara, T.
    • Asian-Australasian Journal of Animal Sciences
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    • 제13권9호
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    • pp.1249-1254
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    • 2000
  • A study was conducted to evaluate the replacement value of Leucaena leucocephala and Gliricidia sepium as nitrogen sources in commercial type supplements for dairy goats. Six crossbred $(Toggenburg{\times}Saanen)$ goats at late stage of lactation were allocated to three dietary treatments in a double $3{\times}3$ Latin square design. The animals were offered rhodes grass (Chloris gayana) hay ad libitum and supplemented with either Leucaena-based concentrate (LBC), Gliricidia-based concentrate (GBC) or commercial based concentrate (CC). Voluntary food intake, milk yield and composition and changes in live weight were measured. The total dry matter (DM) intake was higher (p<0.05) in goats fed GBC than CC (1385 vs 1331 g/d). The DM intake for LBC (1343 g/d) was similar to CC (1331 g/d). The DM intake of hay was also higher (p<0.05) in goats fed GBC (834 g/d) than those receiving LBC or CC (789, 782 g/d, respectively). Animals supplemented with GBC recorded positive (11 g/d) weight gain while the other groups lost weight (13, 19 g/d) for LBC and CC respectively, although these differences were not statistically significant (p>0.05). The composition of milk were: butterfat 58, 49 and 55 g/kg; crude protein 37.0, 35.4 and 36.1 g/kg; lactose 33, 29 and 30 g/kg; Ash 8.5, 8.5 and 7.9 g/kg and total solids 136.5, 121.9 and 129.0 g/kg, for goats fed LBC, GBC and CC respectively. There were no differences in the composition of milk due to these dietary treatments. At the end of performance trial, a digestibility trial was conducted using 6 female goats allocated to the three treatments (LBC, GBC and CC) in an incomplete randomized block design and each goat received a different supplement in each of two successive periods. There were no differences in nutrient digestibility except for DM, which was higher (p<0.05) in CC compared to the other treatments (615, 622, 720 g/kg for LBC, GBC and CC, respectively). Economic analysis showed that CC diet was more expensive (0.20 US$/kg) and had a lower margin over supplementation (0.11 US$) compared to LBC and GBC (0.13 vs 0.12 US$/kg and 01.5 vs 0.12 US$, respectively). It is concluded that the Leucaena and Gliricidia could contribute as nitrogen sources in compounded diet supplements without any detrimental effects on production in dairy goats.

DETERMINATION OF MOISTURE AND NITROGEN ON UNDRIED FORAGES BY NEAR INFRARED REFLECTANCE SPECTROSCOPY(NIRS)

  • Cozzolino, D.;Labandera, M.;Inia La Estanzuela
    • 한국근적외분광분석학회:학술대회논문집
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    • 한국근적외분광분석학회 2001년도 NIR-2001
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    • pp.1620-1620
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    • 2001
  • Forages, both grazed and conserved, provide the basis of ruminant production systems throughout the world. More than 90 per cent of the feed energy consumed by herbivorous animals world - wide were provided by forages. With such world - wide dependence on forages, the economic and nutritional necessity of been able to characterize them in a meaningful way is vital. The characterization of forages for productive animals is becoming important for several reasons. Relative to conventional laboratory procedures, Near Infrared Reflectance Spectroscopy (NIRS) offers advantages of simplicity, speed, reduced chemical waste, and more cost-effective prediction of product functionality. NIR spectroscopy represents a radical departure from conventional analytical methods, in that entire sample of forage is characterized in terms of its absorption properties in the near infrared region, rather than separate subsamples being treated with various chemicals to isolate specific components. This forces the analyst to abandon his/her traditional narrow focus on the sample (one analyte at a time) and to take a broader view of the relationship between components within the sample and between the sample and the population from which it comes. forage is usually analysed by NIRS in dry and ground presentation. Initial success of NIRS analysis of coarse forages suggest a need to better understand the potential for analysis of minimally processed samples. Preparation costs and possible compositional alterations could be reduced by samples presented to the instrument in undried and unground conditions. NIRS has gained widespread acceptance for the analysis of forage quality constituents on dry material, however little attention has been given to the use of NIRS for chemical determinations on undried and unground forages. Relatively few works reported the use of NIRS to determine quality parameters on undried materials, most of them on both grass and corn silage. Only two works have been found on the determination of quality parameters on fresh forages. The objectives of this paper were (1) to evaluate the use of NIRS for determination of nitrogen and moisture on undried and unground forage samples and (2) to explore two mathematical treatments and two NIR regions to predict chemical parameters on fresh forage. Four hundred forage samples (n: 400) were analysed in a NIRS 6500 instrument (NIR Systems, PA, USA) in reflectance mode. Two mathematical treatments were applied: 1,4,4,1 and 2,5,5,2. Predictive equations were developed using modified partial least squares (MPLS) with internal cross - validation. Coefficient of determination in calibration (${R^2}_{CAL}$) and standard error in cross-validation (SECV) for moisture were 0.92 (12.4) and 0.92 (12.4) for 1,4,4,1 and 2,5,5,2 respectively, on g $kg^{-1}$ dry weight. For crude protein NIRS calibration statistics yield a (${R^2}_{CAL}$) and (SECV) of 0.85 (19.8) and 0.85 (19.6) for 1,4,4,1 and 2,5,5,2 respectively, on a dry weight. It was concluded that NIRS is a suitable method to predict moisture and nitrogen on fresh forage without samples preparation.

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근적외선분광분석법에 의한 등숙시기별 보리종실의 성분측정 (Determination of Barley Grain Components at Different Maturing Stages by Near Infrared Reflectance Spectroscopic Analysis)

  • 김병주;박의호;서형수
    • 한국작물학회지
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    • 제41권1호
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    • pp.13-19
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    • 1996
  • 많은 보리계통의 주요 종실성분을 등숙초기단계에 신속하고 정확하게 분석하기 위한 근적외분광분석법의 이용가능성을 검토하기 위하여 겉보리 34계통을 재료로 출수후 25, 30, 35일에 각각 수확하였다. 이들 시료들을 대상으로 양질의 보리품종을 선발하는데 있어 품질면에 크게 영향을 미치는 전분, $\beta$-glucan, 단백질 및 회분성분을 일반분석법에 의해 분석한 뒤, 이 성적과 근적외분광광도계(NIRS)를 이용한 검량식(calibration equation)작성으로, 간접측정방법의 가능성을 검토하였다. 각 등숙시기별 성분별로 우수 검량식을 택하고 이를 이용하여 실분석치와의 상관정도를 검토함으로써 실행정확성을 판단하였는데 그 결과를 요약하면 다음과 같다. 1. 출수후 25일의 보리종실성분 중 전분은 2,163nm의 파장으로 구성된 검량식에서, $\beta$-glucan은 2,006nm 파장의 검량식에서 측정정확도가 높아 NIRS의 이용가능성이 있었으나 단백질 및 회분은 불가능한 것으로 나타났다. 2. 출수후 30일의 보리종실성분 중 전분은 2,053/2,082/2,235nm, $\beta$-glucan은 2,064/2,110nm, 단백질은 2,139/2,167/2,006/1,931nm로 구성된 검량식에서 각각 측정정확도가 높았으나 회분은 실분석치와의 유의성이 인정되지 않았다. 3. 출수후 35일 수확 종실의 경우 NIRS에 의한 전분과 단백질의 측정정확도가 높은 것으로 나타났으나 $\beta$-glucan및 회분은 유의성이 인정되지 않았다. 4. 출수후 35일 종실의 $\beta$-glucan은 흡광도 peak가 2,090nm에서 이루어져 분석이 되지 않았으며, 또한 회분은 세 시기 모두 계통간 성분변이 폭이 좁아 NIRS에 의한 분석이 어려울 것으로 판단되었다.

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정삼투-무삼투압차(△𝜋≤0) 법 역삼투 해수 담수화 및 고농도 NaCl 용액의 삼투압 근사식 (Forward Osmotic Pressure-Free (△𝜋≤0) Reverse Osmosis and Osmotic Pressure Approximation of Concentrated NaCl Solutions)

  • 장호남;최경록;정권수;박권우;김유천;서찰스;김낙종;김도현;김범수;김한민;장윤석;김남욱;김인호;김건우;이햇빛;치앙페이
    • 멤브레인
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    • 제32권4호
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    • pp.235-252
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    • 2022
  • 무삼투압차 역삼투압(Δ𝜋= 0)은 KAIST H. N. Chang 명예교수가 2013년 발명, 2014년 미국 특허 출원, 2018년 특허 취득(US 9,950,297) 해수담수화기술. Chang 등의 RO 기술은 삼투압 조정조와 저압 역삼투압의 2 챔버로 구성. Chang 등은 소금물을 비롯한 모든 수용액은 물과 용질(소금)로 완전 분리 가능 주장. 삼투압차 조정조, 저압 역삼투압조 2 챔버로 구성됨. 고농도 용액의 삼투압은 1908년 미국화학회지 출간된 MIT G. N. Lewis식 이용. 두 번째 특허(US 10,953,3367)에서 RO가 10~12 bar 저 삼투압차 수행 가능 증명. 세 번째 특허(Korea 10-2322755, 해외 출원 중) Singularity ZERO 활용하면 기존 RO에 비해 물은 50% 추가, 막 면적은 1/3, 이론에너지는 1/5, 동일 용량의 S-ZERO 기술은 기존 RO 건설비의 50~60%로 예측됨.

공압식 갱내충전을 위한 모형실험 연구 (A Study on the Model Test for Pneumatic Mine-Filling)

  • 양인제;신동춘;윤병식;목진호;김학성;이상은
    • 터널과지하공간
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    • 제24권6호
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    • pp.449-463
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    • 2014
  • 국내의 경우 슬러리나 페이스트 형태로 수압식 충전에 대한 연구와 다양한 폐광산 현장에 적용한 사례는 많으나, 공압식 충전에 대한 연구는 진행되지 않고 있다. 기존의 공압식 충전법은 강관의 구부러진 곳에서 충전재 유동에 의한 마모에 의해 강관의 손상이 발생한다. 이와 같은 문제점을 개선하기 위하여 새로 고안한 개량형 노즐을 이용한 공압식 충전법을 개발하였다. 본 연구에서는 접근이 가능하거나 불가능한 채굴공동을 모사하여 실험실에서 모형실험을 수행하고 그 충전효율을 평가하였다. 갱내 충전법 실험에서는 토출각의 변화, 모래의 투입량 및 입자 크기에 따라 충전효율을 분석하였으며, 지상 충전법 실험에서는 중력식, 재래식 및 개량형 충전방법의 실험으로부터 충전효율을 분석하여 개량형 공압식 충전법의 우수성을 입증하였다.

보리 사일리지용 미생물의 발효능력 평가 (Evaluation of Fermentation Ability of Microbes for Whole Crop Barley Silage Inoculant)

  • 김종근;함준상;정의수;박형수;이종경;정민웅;최기춘;조남철;서성
    • 한국초지조사료학회지
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    • 제29권3호
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    • pp.235-244
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    • 2009
  • 본 시험은 사일리지 조제시 품질 개선을 위한 젖산균 첨가제를 개발하기 위하여 2000년부터 2002년까지 국립축산과학원 초지사료과 조사료 분석실험실에서 수행하였다. 보리는 한국에서 중요한 식량작물로서 대부분이 식량으로 이용되나 곡실부분이 많아 가축의 사료로도 활용되며 특히 총체보리로 하여 반추가축의 조사료로 활용되고 있다. 본 시험은 우량 보리 사일리지에서 젖산균을 수집하여 0.02% $NaN_2$가 함유된 MRS agar에 도말하여 생장을 보며 1차 선발한 후 MRS broth에서 다시 배양하여 생장 능력과 산생성 능력을 평가하여 총 4종의 균주를 선발하였다. 선발된 균주는 그람 양성, 로드형, 카탈라제를 생성하지 않으며 생화학적 특성과 기질 이용성을 평가한 결과 Lactobacillus plantarum으로 판명되었다. 선발된 균주는 시중 판매되는 첨가제 등과 함께 호숙기의 보리 사일리지에 첨가하여 2개월후 사일리지의 품질을 조사한 결과 B2-2 미생물 처리구애서 pH가 낮았고 젖산함량도 높게 나타났다. 사일리지 품질 점수와 등급에 있어서도 B2-2 처리구가 높게 나타났다. 따라서 이상의 결과를 종합하여 볼 때 B2-2 균주는 보리 사일리지용 우량 미생물로 추천되었다.

CHANGING THE ANIMAL WORLD WITH NIR : SMALL STEPS OR GIANT LEAPS\ulcorner

  • Flinn, Peter C.
    • 한국근적외분광분석학회:학술대회논문집
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    • 한국근적외분광분석학회 2001년도 NIR-2001
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    • pp.1062-1062
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    • 2001
  • The concept of “precision agriculture” or “site-specific farming” is usually confined to the fields of soil science, crop science and agronomy. However, because plants grow in soil, animals eat plants, and humans eat animal products, it could be argued (perhaps with some poetic licence) that the fields of feed quality, animal nutrition and animal production should also be considered in this context. NIR spectroscopy has proved over the last 20 years that it can provide a firm foundation for quality measurement across all of these fields, and with the continuing developments in instrumentation, computer capacity and software, is now a major cog in the wheel of precision agriculture. There have been a few giant leaps and a lot of small steps in the impact of NIR on the animal world. These have not been confined to the amazing advances in hardware and software, although would not have occurred without them. Rapid testing of forages, grains and mixed feeds by NIR for nutritional value to livestock is now commonplace in commercial laboratories world-wide. This would never have been possible without the pioneering work done by the USDA NIR Forage Research Network in the 1980's, following the landmark paper of Norris et al. in 1976. The advent of calibration transfer between instruments, algorithms which utilize huge databases for calibration and prediction, and the ability to directly scan whole grains and fresh forages can also be considered as major steps, if not leaps. More adventurous NIR applications have emerged in animal nutrition, with emphasis on estimating the functional properties of feeds, such as in vivo digestibility, voluntary intake, protein degradability and in vitro assays to simulate starch digestion. The potential to monitor the diets of grazing animals by using faecal NIR spectra is also now being realized. NIR measurements on animal carcasses and even live animals have also been attempted, with varying degrees of success, The use of discriminant analysis in these fields is proving a useful tool. The latest giant leap is likely to be the advent of relatively low-cost, portable and ultra-fast diode array NIR instruments, which can be used “on-site” and also be fitted to forage or grain harvesters. The fodder and livestock industries are no longer satisfied with what we once thought was revolutionary: a 2-3 day laboratory turnaround for fred quality testing. This means that the instrument needs to be taken to the samples rather than vice versa. Considerable research is underway in this area, but the challenge of calibration transfer and maintenance of instrument networks of this type remains. The animal world is currently facing its biggest challenges ever; animal welfare, alleged effects of animal products on human health, environmental and economic issues are difficult enough, but the current calamities of BSE and foot and mouth disease are “the last straw” NIR will not of course solve all these problems, but is already proving useful in some of these areas and will continue to do so.

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