• Asian-Australasian Journal of Animal Sciences
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• 제28권11호
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• pp.1624-1628
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• 2015

Rigorously standardized laboratory protocols are essential for meaningful comparison of data from multiple sites. Considering that interactions of minerals with organic matrices may vary depending on the material nature, there could be peculiar demands for each material with respect to digestion procedure. Acid digestion procedures were evaluated using different nitric to perchloric acid ratios and one- or two-step digestion to estimate the concentration of calcium, phosphorus, magnesium, and zinc in samples of carcass, bone, excreta, concentrate, forage, and feces. Six procedures were evaluated: ratio of nitric to perchloric acid at 2:1, 3:1, and 4:1 v/v in a one- or two-step digestion. There were no direct or interaction effects (p>0.01) of nitric to perchloric acid ratio or number of digestion steps on magnesium and zinc contents. Calcium and phosphorus contents presented a significant (p<0.01) interaction between sample type and nitric to perchloric acid ratio. Digestion solution of 2:1 v/v provided greater (p<0.01) recovery of calcium and phosphorus from bone samples than 3:1 and 4:1 v/v ratio. Different acid ratios did not affect (p>0.01) calcium or phosphorus contents in carcass, excreta, concentrate, forage, and feces. Number of digestion steps did not affect mineral content (p>0.01). Estimated concentration of calcium, phosphorus, magnesium, and zinc in carcass, excreta, concentrated, forage, and feces samples can be performed using digestion solution of nitric to perchloric acid 4:1 v/v in a one-step digestion. However, samples of bones demand a stronger digestion solution to analyze the mineral contents, which is represented by an increased proportion of perchloric acid, being recommended a digestion solution of nitric to perchloric acid 2:1 v/v in a one-step digestion.

• International Journal of Fluid Machinery and Systems
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• 제8권4호
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• pp.264-273
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• 2015

This presentation describes an experimental approach for the detection of cavitation in hydraulic machines by use of ultrasonic signal analysis. Instead of using the high frequency pulses (typically 1MHz) only for transit time measurement different other signal characteristics are extracted from the individual signals and its correlation function with reference signals in order to gain knowledge of the water conditions. As the pulse repetition rate is high (typically 100Hz), statistical parameters can be extracted of the signals. The idea is to find patterns in the parameters by a classifier that can distinguish between the different water states. This classification scheme has been applied to different cavitation sections: a sphere in a water flow in circular tube at the HSLU in Lucerne, a NACA profile in a cavitation tunnel and two Francis model test turbines all at LMH in Lausanne. From the signal raw data several statistical parameters in the time and frequency domain as well as from the correlation function with reference signals have been determined. As classifiers two methods were used: neural feed forward networks and decision trees. For both classification methods realizations with lowest complexity as possible are of special interest. It is shown that two to three signal characteristics, two from the signal itself and one from the correlation function are in many cases sufficient for the detection capability. The final goal is to combine these results with operating point, vibration, acoustic emission and dynamic pressure information such that a distinction between dangerous and not dangerous cavitation is possible.

• Mycobiology
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• 제49권3호
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• pp.223-234
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• 2021

Itajahya rosea was found growing in association with Leucaena leucocephala plants at Savitribai Phule Pune University campus in India. The species identity was confirmed by phylogenetic analysis based on ITS and LSU regions of rDNA, wherein, our fugus was placed along with I. rosea in phylogenetic tree. It represents first record of I. rosea from India. Frequent visitation by Drosophila species on I. rosea fruiting body particularly on gleba was observed. The Drosophila got attracted to the detached gleba under the laboratory conditions and even sometimes, they prefer to sit over the gleba as compare to their food banana. It suggested that I. rosea gleba or pseudostipe produces some compounds for attraction and feeding behavior of Drosophila species. Therefore, we characterized the volatile attractants produced by gleba and pseudostipe of I. rosea by GC-MS analysis. Nineteen compounds were identified from gleba while nine compounds were recovered from the pseudostipe. Out of them, blends of three abundant odor producing volatile compounds were reported namely, Hexadecane, Pentadecane and Nonadecane, which are responsible for attraction of Drosophila toward the gleba. Three fatty acids namely 9,12-octadecadienoic acid (Z,Z), hexadecanoic acid and benzoic acid ethyl ester produced are served as an appetitive signal through olfactory response of Drosophila, so the flies were feed on the gleba. Two pheromones' compounds, heneicosane and (+)-(5S,9S)-5,9-dimethylpentadecane, were also reported in pseudostipe and gleba, respectively, which play a role in Drosophila for breeding. Our study highlights an intriguing chemical ecology of fungus-Drosophila interaction.

• 대한토목학회논문집
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• 제41권4호
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• pp.399-404
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• 2021

2020년 1월 1일부터 국제해사기구(International Maritime Organization, IMO)는 선박 대기오염 규제를 지속적으로 강화하고 있으며, 배출 규제해역이 아닌 일반해역을 운항하는 전 세계 모든 적용대상 선박에 대하여 황함유량 0.5 %를 초과하지 않는 연료유를 사용하거나 이에 준하는 대기오염 배출기준을 만족하도록 결정하였다. 최근 습식 스크러버를 통한 본 규정의 대기오염 배출기준을 만족시키려는 연구가 진행되고 있으나, 이 기술은 폐세정수를 동시에 유발하는 문제점이 있다. 본 연구에서는 국제해사기구의 폐세정수 배출 기준을 준수하기 위한 이온교환수지 공정의 성능을 평가하였다. 모사폐세정수를 사용하여 실험실 규모의 회분식 및 연속식 실험을 진행하였다. 실험 결과 모사폐세정수의 높은 총용존고형물에도 불구하고 이온교환수지 특성에 따라 선택적으로 질산염의 제거가 효율적으로 이루어짐을 확인하였다. 추가적으로 다양한 운영조건을 최적화함에 따라서 제거 효율을 개선할 수 있었으며, 이를 통하여 국제해사기구의 폐세정수 배출수 수질 기준을 만족할 수 있을 것으로 판단된다.

• 한국식품영양학회지
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• 제26권4호
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• pp.737-744
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• 2013

오늘날 현대인들의 식생활방식 중 과다한 열량의 영양소 섭취로 인하여 초래되는 비만의 유발 원인 중의 하나로 알려진 고지혈증(hyperlipidemia)에 대한 예방효과가 있는 것으로 알려진 전통방식으로 제조된 고추장의 생리활성 기능성 효과를 분석하고자 하였다. 이 연구에서는 실험동물로서 Sprague-Dawley(SD)-Rat에 대해서 고추장 추출물의 건강증진 효과에 대해서 실험동물을 사용하여 직접 확인하고 시도하였다. 실험군은 실험동물용 일반사료만을 먹인 음성대조군 그룹(N-group)과 일반사료와 고추장 추출물을 동시에 먹인 시험그룹(K-group) 그리고 양성대조군으로 고콜레스테롤 사료만을 먹인 그룹(H-group)으로 구분하여 5주간 사육하면서 건강증진효과의 발현에 대해서 분석하였다. 3그룹의 실험동물을 5주간의 사육 후 해부했을 때 H-group의 모든 동물(100%)에서 공통적으로 심한 지방간(fatty liver) 소견이 관찰되었으나, N-group이나 K-group의 어떠한 동물에서도 지방간 소견은 관찰되지 않았다. 한편, 실험동물의 혈장성분에 대한 혈액생화학적 성적의 비교에서는 고지혈증의 예방에 유익한 성분으로 알려진 HDL의 경우, 고추장 추출물 투여 그룹($25{\pm}8.43mg/d{\ell}$)은 H-group($22.6{\pm}4.16$) 보다 다소간 상승된 수치를 보였으나, 특히 고지혈증을 악화시키는 원인으로 알려진 LDL 경우에는 H-group ($55.8{\pm}44.8mg/d{\ell}$)이 고추장 추출물 투여 그룹($8.6{\pm}1.52mg/d{\ell}$)과 음성대조군인 N-group($9.8{\pm}2.39$)의 성적보다도 유의적으로 높은 수치를 나타냈다. 결과적으로 이 연구에서 SD-Rat에 투여했던 고추장 추출물은 실험동물에서 건강기능 개선효과의 하나인 저지방혈증 효과를 발현한 것으로 사료되었다.

• Animal Bioscience
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• 제35권11호
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• pp.1698-1710
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• 2022

Objective: Raw potato starch (RPS) is resistant to digestion, escapes absorption, and is metabolized by intestinal microflora in the large intestine and acts as their energy source. In this study, we compared the effect of different concentrations of RPS on the intestinal bacterial community of weaned piglets. Methods: Male weaned piglets (25-days-old, 7.03±0.49 kg) were either fed a corn/soybean-based control diet (CON, n = 6) or two treatment diets supplemented with 5% RPS (RPS5, n = 4) or 10% RPS (RPS10, n = 4) for 20 days and their fecal samples were collected. The day 0 and 20 samples were analyzed using a 16S rRNA gene sequencing technology, followed by total genomic DNA extraction, library construction, and high-throughput sequencing. After statistical analysis, five phyla and 45 genera accounting for over 0.5% of the reads in any of the three groups were further analyzed. Furthermore, short-chain fatty acids (SCFAs) in the day 20 fecal samples were analyzed using gas chromatography. Results: Significant changes were not observed in the bacterial composition at the phylum level even after 20 d post feeding (dpf); however, the abundance of Intestinimonas and Barnesiella decreased in both RPS treatment groups compared to the CON group. Consumption of 5% RPS increased the abundance of Roseburia (p<0.05) and decreased the abundance of Clostridium (p<0.01) and Mediterraneibacter (p< 0.05). In contrast, consumption of 10% RPS increased the abundance of Olsenella (p<0.05) and decreased the abundance of Campylobacter (p<0.05), Kineothrix (p<0.05), Paraprevotella (p<0.05), and Vallitalea (p<0.05). Additionally, acetate (p<0.01), butyrate (p<0.05), valerate (p = 0.01), and total SCFAs (p = 0.01) were upregulated in the RPS5 treatment group Conclusion: Feeding 5% RPS altered bacterial community composition and promoted gut health in weaned piglets. Thus, resistant starch as a feed additive may prevent diarrhea in piglets during weaning.

• 한국초지조사료학회지
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• 제34권4호
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• pp.277-282
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• 2014

Nutritive value analysis of feed is very important for the growth of livestock, and ensures the efficiency of feeds as well as economic status. However, general laboratory analyses require considerable time and high cost. Near-infrared reflectance spectroscopy (NIRS) is a spectroscopic technique used to analyze the nutritive values of seeds. It is very effective and less costly than the conventional method. The sample used in this study was a corn kernel and the partial least square regression method was used for evaluating nutrient composition, digestibility, and energy value based on the calibration equation. The evaluation methods employed were the coefficient of determination ($R^2$) and the root mean squared error of prediction (RMSEP). The results showed the moisture content ($R^2_{val}=0.97$, RMSEP=0.109), crude protein content ($R^2_{val}=0.94$, RMSEP=0.212), neutral detergent fiber content ($R^2_{val}=0.96$, RMSEP=0.763), acid detergent fiber content ($R^2_{val}=0.96$, RMSEP=0.142), gross energy ($R^2_{val}=0.82$, RMSEP=23.249), in vitro dry matter digestibility ($R^2_{val}=0.68$, RMSEP=1.69), and metabolizable energy (approximately $R^2_{val}$ >0.80). This study confirmed that the nutritive components of corn kernels can be predicted using near-infrared reflectance spectroscopy.

• 한국해안해양공학회지
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• 제5권4호
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• pp.329-337
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• 1993

파낭수조를 이용하는 수리실험에서 실험결과들 사이에 동질성 및 호환성을 유지하는 것은 매우 중요한 문제이며 이는 목적파낭을 얼마나 장시간 수조내에 유지시킬 수 있는가에 달려있다. 본고에서는 계측파랑을 목적파랑에 일치시키기 위한 순환성 제어회로를 구성하고 회로해석을 통하여 컴퓨터로 용이하게 조파기 하드웨어를 제어할 수 있는 알고리즘을 제시하였다. 알고리즘의 주된 기능으로서 시간영역에서 이산적으로 적용되는 반사파 흡수필터를 포함하였다. 필터의 구현방식은 이산적 입력과 출력이 직접대응하는 방식이므로 데이타의 통계적 성격이 사전에 알려져 있지 않은 불확정적 입력데이타에 대해서도 적용이 가능하다. 예제해석을 통하여 알고리즘을 수치시험해 분 결과, 임의의 규칙 또는 불규칙 반사파 시그널에 대해서 본 제어회로의 반사파 흡수기능이 매우 탁월함을 입증하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제31권8호
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• pp.1252-1258
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• 2018

Objective: This study was carried out to determine the effects of cumin essential oil on the silage fermentation, aerobic stability and in vitro digestibility of alfalfa silages. Methods: Alfalfa was harvested at early bloom (5th cutting) stage in October and wilted for about 3 hours. The research was carried out at three groups which were the control group where no additive control was done (CON), cumin essential oil (CMN3) with 300 mg/kg and CMN5 with 500 mg/kg cumin essential oil addition. Alfalfa was ensiled in plastic bags. The packages were stored at $8^{\circ}C{\pm}2^{\circ}C$ under laboratory conditions. All groups were sampled for physical, chemical and microbiological analysis 120th day after ensiling. At the end of the ensiling period, all silages were subjected to an aerobic stability test for 7 days. In addition, enzimatic solubility of organic matter (ESOM), metabolizable energy (ME), and relative feed value (RFV) of these silages were determined. Results: pH level decreased in the cumin groups compared to CON (p<0.05), thus inhibiting proteolytic enzymes from breaking down proteins into ammonia. In addition, it increased ESOM amount, and concordantly provided an increase of ME contents. Similarly, dry matter intake and RFV ratio increased. After opening the silage, it kept its aerobic stability for three days. Conclusion: Cumin essential oil improved fermentation, and affected chemical and microbiological characteristics of silages. Especially the addition of 300 mg/kg cumin provided cell wall fractionation through stimulating the activities of enzymes responsible. It also increased the number and activity of lactic acid bacteria (LAB) through providing a development of LAB.

• Journal of Animal Science and Technology
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• 제62권6호
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• pp.884-892
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• 2020

Corticosterone is known as a biological stress index in many species including birds. Feather corticosterone concentration (FCC) has increasingly been used as a measure for chronic stress status in broiler chickens. As sample preparation is the first step of analytical process, different techniques of feather matrix disruption need to be validated for obtaining better result in analysing corticosterone extraction. The current study was a validation of pulverizing the feather by bead beater (BB) and surgical scissors (SS) processing prior to corticosterone extraction in feather of broiler chickens. The type of feather processing prior to the hormone extraction may alter the final output. Thereby, finding a standard method according to laboratory facilities is pivotal. This study carried out to determine the effects of feather pulverization methods on the extraction amount of corticosterone in broiler chickens. Feathers were sampled from four weeks old Ross 308 broiler chickens (n = 12 birds). All broiler chickens were kept under the same environmental condition and had access to feed and water. Feather samples were assigned to one of the following processing methods 1) using a BB for pulverizing and 2) using a SS for chopping into tiny pieces. Each sample was duplicated into two wells during enzyme immunoassay (EIA) analysis to improve the accuracy of the obtained data. The results showed lower standard errors and constant output of FCC by using the BB method compared with the SS method. Overall comparison of FCC showed a significantly higher (p < 0.001) amount of the FCC in the BB compared with the SS. Overall, using the BB method is recommended over the SS method for feather processing due to the ability to homogenize a large number of samples simultaneously, ease of use and greater extraction of feather corticosterone.