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http://dx.doi.org/10.5187/jast.2020.62.6.884

Effects of feather processing methods on quantity of extracted corticosterone in broiler chickens  

Ataallahi, Mohammad (College of Animal Life Sciences, Kangwon National University)
Nejad, Jalil Ghassemi (Team of an Educational Program for Specialists in Global Animal Science, Brain Korea 21 Plus Project, Sanghuh College of Life Sciences, Konkuk University)
Song, Jun-Ik (Division of Animal Husbandry, Yonam College)
Kim, Jin-Soo (College of Animal Life Sciences, Kangwon National University)
Park, Kyu-Hyun (College of Animal Life Sciences, Kangwon National University)
Publication Information
Journal of Animal Science and Technology / v.62, no.6, 2020 , pp. 884-892 More about this Journal
Abstract
Corticosterone is known as a biological stress index in many species including birds. Feather corticosterone concentration (FCC) has increasingly been used as a measure for chronic stress status in broiler chickens. As sample preparation is the first step of analytical process, different techniques of feather matrix disruption need to be validated for obtaining better result in analysing corticosterone extraction. The current study was a validation of pulverizing the feather by bead beater (BB) and surgical scissors (SS) processing prior to corticosterone extraction in feather of broiler chickens. The type of feather processing prior to the hormone extraction may alter the final output. Thereby, finding a standard method according to laboratory facilities is pivotal. This study carried out to determine the effects of feather pulverization methods on the extraction amount of corticosterone in broiler chickens. Feathers were sampled from four weeks old Ross 308 broiler chickens (n = 12 birds). All broiler chickens were kept under the same environmental condition and had access to feed and water. Feather samples were assigned to one of the following processing methods 1) using a BB for pulverizing and 2) using a SS for chopping into tiny pieces. Each sample was duplicated into two wells during enzyme immunoassay (EIA) analysis to improve the accuracy of the obtained data. The results showed lower standard errors and constant output of FCC by using the BB method compared with the SS method. Overall comparison of FCC showed a significantly higher (p < 0.001) amount of the FCC in the BB compared with the SS. Overall, using the BB method is recommended over the SS method for feather processing due to the ability to homogenize a large number of samples simultaneously, ease of use and greater extraction of feather corticosterone.
Keywords
Bead beater; Broiler chicken; EIA; Feather corticosterone methodology; Surgical scissors;
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1 Lattin CR, Reed JM, DesRochers DW, Romero LM. Elevated corticosterone in feathers correlates with corticosterone-induced decreased feather quality: a validation study. J Avian Biol. 2011;42:247-52. https://doi.org/10.1111/j.1600-048X.2010.05310.x   DOI
2 Adamkova M, Bilkova Z, Tomasek O, Simek Z, Albrecht T. Feather steroid hormone concentrations in relation to age, sex, and molting time in a long-distance migratory passerine. Ecol Evol. 2019; 9:9018-26. https://doi.org/10.1002/ece3.5447   DOI
3 Romero LM, Fairhurst GD. Measuring corticosterone in feathers: strengths, limitations, and suggestions for the future. Comp Biochem Physiol A Mol Integr Physiol. 2016; 202:112-22. https://doi.org/10.1016/j.cbpa.2016.05.002   DOI
4 Meyer J, Novak M, Hamel A, Rosenberg K. Extraction and analysis of cortisol from human and monkey hair. J Visualized Exp. 2014;83:e50882. https://doi.org/10.3791/50882   DOI
5 Dubacq S. Performing efficient sample preparation with hard tumor tissue: Precellys® bead-beating homogenizer solution. Nat Methods. 2016;13:i-ii. https://doi.org/10.1038/nmeth.f.394   DOI
6 Gibbons LE, Brangs HC, Burden DW. Bead beating: a primer. Ran Primers. 2014;12:1-20.
7 Dhouailly D. A new scenario for the evolutionary origin of hair, feather, and avian scales. J Anat. 2009;214:587-606. https://doi.org/10.1111/j.1469-7580.2008.01041.x   DOI
8 Davenport MD, Tiefenbacher S, Lutz CK, Novak MA, Meyer JS. Analysis of endogenous cortisol concentrations in the hair of rhesus macaques. Gen Comp Endocrinol. 2006;147:255-61. https://doi.org/10.1016/j.ygcen.2006.01.005   DOI
9 Burnett TA, Madureira AML, Silper BF, Nadalin A, Tahmasbi A, Veira DM, et al. Short communication: factors affecting hair cortisol concentrations in lactating dairy cows. J Dairy Sci. 2014;97:7685-90. https://doi.org/10.3168/jds.2014-8444   DOI
10 Mostl E, Rettenbacher S, Palme R. Measurement of corticosterone in birds' droppings: an analytical approach. Ann N Y Acad Sci. 2005;1046:17-34. https://doi.org/10.1196/annals.1343.004   DOI
11 Newman AEM, Pradhan DS, Soma KK. Dehydroepiandrosterone and corticosterone are regulated by season and acute stress in a wild songbird: jugular versus brachial plasma. Endocrinology. 2008;149:2537-45. https://doi.org/10.1210/en.2007-1363   DOI
12 Slominski R, Rovnaghi CR, Anand KJ. Methodological considerations for hair cortisol measurements in children. Ther Drug Monit. 2015;37:812-20. https://doi.org/10.1097/FTD.0000000000000209   DOI
13 Koren L, Mokady O, Karaskov T, Klein J, Korent G, Geffen E. A novel method using hair for determining hormonal levels in wildlife. Anim Behav. 2002;63:403-6. https://doi.org/10.1006/anbe.2001.1907   DOI
14 Accorsi PA, Carloni E, Valsecchi P, Viggiani R, Gamberoni M, Tarnanini C, et al. Cortisol determination in hair and faeces from domestic cats and dogs. Gen Comp Endocrinol. 2008;155:398-402. https://doi.org/10.1016/j.ygcen.2007.07.002   DOI
15 Ghassemi Nejad J, Lohakare JD, Son JK, Kwon EG, West JW, Sung KI. Wool cortisol is a better indicator of stress than blood cortisol in ewes exposed to heat stress and water restriction. Animal. 2014;8:128-132. https://doi.org/10.1017/S1751731113001870   DOI
16 Wingfield JC, Vleck CM, Moore MC. Seasonal changes of the adrenocortical response to stress in birds of the Sonoran desert. J Exp Zool. 1992;264:419-28. https://doi.org/10.1002/jez.1402640407   DOI
17 Bortolotti GR, Marchant T, Blas J, Cabezas S. Tracking stress: localisation, deposition and stability of corticosterone in feathers. J Exp Biol. 2009;212:1477-82. https://doi.org/10.1242/jeb.022152   DOI
18 Carbajal A, Tallo-Parra O, Sabes-Alsina M, Mular I, Lopez-Bejar M. Feather corticosterone evaluated by ELISA in broilers: a potential tool to evaluate broiler welfare. Poult Sci. 2014;93:2884-6. https://doi.org/10.3382/ps.2014-04092   DOI
19 Khan MZ, Altmann J, Isani SS, Yu J. A matter of time: evaluating the storage of fecal samples for steroid analysis. Gen Comp Endocrinol. 2002;128:57-64. https://doi.org/10.1016/S0016-6480(02)00063-1   DOI
20 Romero LM, Romero RC. Corticosterone responses in wild birds: the importance of rapid initial sampling. Condor. 2002;104:129-35. https://doi.org/10.1093/condor/104.1.129   DOI
21 Sheriff MJ, Dantzer B, Delehanty B, Palme R, Boonstra R. Measuring stress in wildlife: techniques for quantifying glucocorticoids. Oecologia. 2011;166:869-87. https://doi.org/10.1007/s00442-011-1943-y   DOI
22 Ghassemi Nejad J, Ataallahi M, Park KH. Methodological validation of measuring Hanwoo hair cortisol concentration using bead beater and surgical scissors. J Anim Sci Technol. 2019;61:41-6. https://doi.org/10.5187/jast.2019.61.1.41   DOI
23 Harris CM. Corticosterone in feathers as a biomarker: biological relevance, considerations and cautions [Master's theses]. Windsor, ON: University of Windsor; 2015.
24 Harris CM, Madliger CL, Love OP. An evaluation of feather corticosterone as a biomarker of fitness and an ecologically relevant stressor during breeding in the wild. Oecologia. 2017;183:987-96. https://doi.org/10.1007/s00442-017-3836-1   DOI
25 Freeman NE, Newman AEM. Quantifying corticosterone in feathers: validations for an emerging technique. Conserv Physiol. 2018;6:coy051. https://doi.org/10.1093/conphys/coy051   DOI
26 Blas J. Stress in birds. In Scanes CG, editor. Sturkie's avian physiology. New York, NY: Academic Press; 2015. p. 769-810.
27 Weimer SL, Wideman RF, Scanes CG, Mauromoustakos A, Christensen KD, Vizzier-Thaxton Y. An evaluation of methods for measuring stress in broiler chickens. Poult Sci. 2018;97:3381-9. https://doi.org/10.3382/ps/pey204   DOI
28 Greenhouse Gas Inventory and Research Center. Fourth National Communication of the Republic of Korea under the United Nation Framework Convention on Climate Change [Internet]. The Government of Republic of Korea. 2019 [cited 2020 Jul 3]. http://www.gir.go.kr/eng/board/read.do?pagerOffset=0&maxPageItems=10%20&maxIndexPages=10&searchKey=&-searchValue=&menuId=14&boardId=37&boardMasterId=12&boardCategoryId=
29 Korea Meteorological Administration. 2018 annual climate report [Internet]. 2018 [cited 2020 Jul 3]. https://www.kma.go.kr/download_01/Annual_Report_2018.pdf
30 Allen MR, Dube OP, Solecki W, Aragon-Durand F, Cramer W, Humphreys S, et al. Framing and context. In: Masson-Delmotte V, Zhai P, Portner HO, Roberts D, Skea J, Shukla PR, Pirani A, et al., editors. Global Warming of 1.5℃: an IPCC Special Report on the impacts of global warming of 1.5℃ above pre-industrial levels and related global greenhouse gas emission pathways, in the context of strengthening the global response to the threat of climate change, sustainable development, and efforts to eradicate poverty. Geneva: Intergovernmental Panel on Climate Change; 2018. p. 47-92.
31 Bortolotti GR, Marchant TA, Blas J, German T. Corticosterone in feathers is a long-term, integrated measure of avian stress physiology. Funct Ecol. 2008;22:494-500. https://doi.org/10.1111/j.1365-2435.2008.01387.x   DOI