• 제목/요약/키워드: feces

검색결과 1,464건 처리시간 0.022초

20대와 60대에서 사상체질 완실무병 지표 소화 대변 땀 소변의 특징 비교 (Comparison of Digestion, Feces, Sweat and Urination according to Sasang Constitution in the 20s and 60s)

  • 김구;오승윤;주종천;장은수;이시우
    • 사상체질의학회지
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    • 제22권2호
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    • pp.70-81
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    • 2010
  • 1. Objectives: The purpose of this study was verify the difference in digestion, feces, sweat and urination characteristics according to Sasang Constitution among subjects in the age range of their 20s and 60s. 2. Methods: Three hundred and thirty eight participants were recruited in the age range of their 20s and 264 participants in the age range of their 60s. All the participants' Sasang Constitutions were diagnosed by Sasang Constitutional medicine expert. Then their digestion, feces, sweat and urination characteristics were assessed through the questionnaire. 3. Results: 1)Regarding digestion, Soeumin in all age ranges had bad appetites and Soeumin in their 20s had low digestibility. 2)Regarding feces, the daily defecation frequency showed no difference according to constitution. Only Soeumin in their 20s showed diarrheal state of stool. 3)Regarding sweat, Taeeumin usually perspired more in their 20s and there was no difference in the feeling after sweating according to age and constitution. 4)Regarding urination, frequency and strength showed no difference according to age and constitution. 4. Conclusions: Some difference was observed in the digestion, feces and sweat characteristics according to Sasang Constitution but these characteristics were susceptible to change in different age ranges. There was no difference in urination according to constitution.

비침습적 분변 샘플을 이용한 우리나라 월동 기러기류의 유전분석 (Use of Genetic Techniques to Analyze Wintering Population of Geese in Korea with Noninvasive Feces Samples)

  • 김민경;원용진;이상돈
    • 환경생물
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    • 제26권3호
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    • pp.264-269
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    • 2008
  • 본 연구는 비침습적 분변 샘플을 이용하여 기러기류의 유전분석이 가능한지 알아보고자 수행되었다. 또한 본 연구에서는 분변을 이용한 연구의 실험적 효율성을 높이는 방법이 요구되었다. 따라서 2007년 한국에서 월동하는 겨울 기러기류의 분변을 서로 다른 두 집단에서 채집하였고, 최종적으로 총 32개 분변 샘플이 분자유전학적 기법으로 분석되었다. 그 결과 총 14개의 haplotype이 나타났으며, 이는 쇠기러기 (Anser albifrons)와 큰기러기(Anser fabalis)그룹으로 분류되었다. 분변을 이용한 연구는 야생에 서식하고 있는 종에게 직접적인 위해를 가하지 않는 방법으로 큰 의미가 있다. 본 연구를 통하여 분변 샘플을 이용한 분자유전학적 실험의 효율성을 높일 수 있는 연구방법이 찾아졌으며, 또한 이 결과는 비침습적 분변 샘플을 이용한 우리나라 월동 기러기류의 첫 번째 연구로서 그 의의가 있다.

DNA Extraction from Protozoan Oocysts/Cysts in Feces for Diagnostic PCR

  • Hawash, Yousry
    • Parasites, Hosts and Diseases
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    • 제52권3호
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    • pp.263-271
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    • 2014
  • PCR detection of intestinal protozoa is often restrained by a poor DNA recovery or by inhibitors present in feces. The need for an extraction protocol that can overcome these obstacles is therefore clear. $QIAamp^{(R)}$ DNA Stool Mini Kit (Qiagen) was evaluated for its ability to recover DNA from oocysts/cysts directly from feces. Twenty-five Giardia-positive, 15 Cryptosporidium-positive, 15 Entamoeba histolytica-positive, and 45 protozoa-free samples were processed as control by microscopy and immunoassay tests. DNA extracts were amplified using 3 sets of published primers. Following the manufacturer's protocol, the kit showed sensitivity and specificity of 100% towards Giardia and Entamoeba. However, for Cryptosporidium, the sensitivity and specificity were 60% (9/15) and 100%, respectively. A series of optimization experiments involving various steps of the kit's protocol were conducted using Cryptosporidium-positive samples. The best DNA recoveries were gained by raising the lysis temperature to the boiling point for 10 min and the incubation time of the InhibitEX tablet to 5 min. Also, using a pre-cooled ethanol for nucleic acid precipitation and small elution volume ($50-100{\mu}l$) were valuable. The sensitivity of the amended protocol to Cryptosporidium was raised to 100%. Cryptosporidium DNA was successfully amplified by either the first or the second primer set. When applied on parasite-free feces spiked with variable oocysts/cysts counts, ${\approx}2$ oocysts/cysts were theoretically enough for detection by PCR. To conclude, the Qiagen kit with the amended protocol was proved to be suitable for protozoan DNA extraction directly from feces and support PCR diagnosis.

돼지 분변 및 도체에서 분리한 대장균, 장구균의 항생제 내성율 조사 (Surveillance of antimicobial resistance ratio of E. coli and Enterococcus spp. isolated from fecal and carcasses of pigs in slaughterhouse)

  • 정귀옥;허정호;이종민;윤이란;최유정;김종수
    • 한국동물위생학회지
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    • 제33권3호
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    • pp.241-248
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    • 2010
  • The present study was conducted to investigate isolation and antimicrobial resistance ratio of E. coli, E. faecium and E. faecalis from feces(l50 samples) and carcasses (150 samples) on slaughtered pigs from 6 slaughterhouse of 13 cities in the Gyeongnam during the period from January 2009 to December 2009. Isolation ratio of E. coli from feces and carcasses were 98 (65.3%) and 110(73.3%), respectively, and simultaneously, E. faecalis and E. faecium from feces and carcasses were isolated 21 (14%), 52(34.7%) and 18(12%), 14 (9.3%), respectively. All E. coli isolated from feces and carcasses except cefepime (0%) and ceftiofur (0%) were exhibited 2.4~83.6% of resistance to teteracycline (83.6%), ampicillin (68.2%), streptomycin (60%), chloram-phenicol (53.8%) and cephalothin (2.4%). All E. faecalis isolated from feces and carcasses except penicillin(0%) and vancomycin (0%) were exhibited 2.7~80.8% of resistance to teteracycline (80.8%), quinupristin/dalfopristin (78%), erythromycin (56.1%), streptomycin (43.8%) and bacitracin (2.7%). All E. faecium isolated from feces and carcasses except gentamicin (0%), vancomycin (0%), florfenicol (0%), linezloid (0%) and bacitracin (0%) were exhibited 3.1~53.1% of resistance to rifampin (53.1%), erythromycin and tetracycline (25%), penicillin (15.6%), ciprofloxacin (9.3%), and streptomycin, chloramphenicol, and quinupristin/dalfopristin (3.1%). According to the heard size, resistance ratio of E. coli strains isolated from feces and carcasses in slaughtered pigs-breeding farms over 1,500 heard to tetracycline, ampicillin, streptomycin and chloramphenicol showed higher resistance ratio (1.0~16.8%) than those of farms-breeding under 1,500 heard. From the our results, we suggest that a few of antimicrobials were used in the Gyeongnam than the other cities.

알긴산과 셀룰로오스 및 펙틴 수준이 흰쥐의 대장기능에 미치는 영향 (Effects of Alginic Acid, Cellulose and Pectin Level on Bowel Function in Rats)

  • 이형자
    • Journal of Nutrition and Health
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    • 제30권5호
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    • pp.465-477
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    • 1997
  • The purpose of this article is to know the effects on bowel function of the kind of fiber and the amount of fiber in SD-rats. To do this experiment, we select of $\alpha$-cellulose as n insoluble cellulose source and alginic acid and pectin as soluble cellulose source. The rats diets contained callolose camcentrations of 1.0%, 3.6%, 6.0% and 10.0%. After that, we raised the SD-rats for 4weeks and measured the amount of food intake, body weight, the food effciency ratio, the length of liver and stomach the weight of the intestines, the transit time through the intestines, pH in feces, and the amount of bile acid and Ca, Mg, pp. 1) The amount of food intake was 15.75-31.00g/day. It was highest in the 10.0% cellulose group and the lowest in the 3.6% and 6.0% alginic acid group (p<0.05). The body weights of rats were 277.50-349.809. It was highest in the 1.0% pectin group and lowest in the 3.6% alginic acid group, 6.0% cellulose group, and 10.0% pectin group. It had differences according to the content fiber and the kind of dietary(p<0.01). The food efficiency ratio was (p<0.01). The higher the content of dietary fiber, the lower the calory and the food efficiency ratio. 2) Transit time was 446.0-775.0 minutes and it showed signidicant ifferences according to the content and kind of dietary fiber(p<0.01). It was long in the 1.0% cellulose group and 1.0% pectin group but short in the 10.0% alginic acid group. As the content of dietary fiber increased, the transit time through the intestines was shortened. The length of small intestine was 101.03-120.40cm and there were no difference cegardloss of the content and kind of fiber. The length of the large intestine was 20.92-25.42cm and there were significant differences according to the content and kind of the fiber. High-fiber diets resulted in increases in the length of the large intestine. 3) The weight of the liver was 8.68-10.96g and there were no differences according to the content and kind of fiber. The weight of stomach was 1.28-1.74g and there were no differences resulting from the kind of dietary fiber, but it was highest in the 10.0% alginic acid group. The weight of the small intestine was 5.52-8.04g with no difference resulting from to the kind of fiber. It was highest in the 10.0% the alginic acid group and lowest in the 1.0% alginic acid group(p<0.05). The weight of large intestine was 2.50-3.30g with no differences related to the kind of dietary fiber. It was heaviest in the 6.0% and 10.0% alginic acid groups and in the 10.0% pectin group with differences related to the content of fiber(p<0.05). 4) The pH of the feces was 5.82-6.86 according to the kind of dietary fiber, alginic acid group was high at 6.66, the cellulose group was 6.26. but the pectin group was low at 6.30. There were difference according to the content of fiber, but no consistency. The content of bile acid was 6.25-34.77umol per 1g of dry feces. According to the kind of dietary fiber, the alginic acid group was low at 12.91umol, cellulose group was 18.64umol and, the pectin group was the highest at 27.78umol(p<0.001). Based on the content of dietary fiber, alginic acid group was low at 1.0%, but high at 3.6% pectin group(p<0.001). 5) The amount of feces was 1.00-5.10g/day. The weight of rat feces was 2.23g/day in the alginic acid goup, 2.75g/day in the cellulose group, and 1.82g/day in the pectin group. According to the content of fiber, cellulose group was high at 10.0% but alginic acid group was 1.0%, and there were significant difference according to the dietary fiber. The more the content of fiber, the more increase the content of feces in alginic acid, cellulose and pectin group. The content of Ca in the feces was 80.10-207.82mg/1g of dry feces. In the dietary fiber, alginic acid group was 193.08mg, cellulose group was 87.5mg, pectin group was 138.16mg. In the content of fiber, alginic acid group was high at 1.0% and 3.6% but low at 10.0% of Pectin group. The content of Mg was 19.15-44.72mg/1g of dry feces. According to the kind of dietary fiber, alginic acid group was 35.33mg, cellulose group was 23.60mg, and pectin was 36.93mg. According to the content of fiber, pectin group was high at 1.0% and low at 10.0% of cellulose group. The content of P was 1.65-4.65mg/1g of dry feces. According to the kind of dietary fiber, alginic acid group 2.23mg/g dry feces, cellulose group was 2.29mg/g, pectin group wa 4.08mg/g dry feces. In the content of fiber, pectin group was high at 6.0% and low at 6.0% alginic acid group, but there were significant difference among the analysis value. The conetnt of Ca and MG was higher in soluble alginic acid group and pectin group than in insoluble cellulose group. The high the content of the dietary fiber, the lower the food efficiency ratio and the short the transit time through intestine with the increase of the length of large intestin as well as the higher level of the stomach, the small intestine and the large intestine. According to the content of the dietary fiber, the amount of the feces, Ca, Mg and P was increased but the length the small intestin, the weight of liver, pH of the feces and the amount of bile acid showed no differences and consistency.

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돼지증식성회장염 신속검진 기술개발(1) - 돼지 분변에서의 로소니아균 검출을 위한 항원, 항체 준비 - (Development of rapid diagnosis technology for porcine proliferative enteropathy (1) - Preparation of the samples and antibody for rapid detecting the lawsonia in pig feces -)

  • 김혁주;홍종태;유병기;김기영;이진주;김석
    • Journal of Biosystems Engineering
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    • 제37권6호
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    • pp.420-428
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    • 2012
  • Purpose: Porcine proliferative enteropathy(PPE), caused by the obligate intracellular bacterium Lawsonia intracellularis, is a widely distributed disease throughout the world causing substantial economic loss. The bacterial pathogen invades the intestinal epithelial cells which causes hyperplasia of the infected cells and leads to the process of disease pathogenesis. For diagnosing PPE in a pig farm in earlier stage, a rapid diagnosing test equipment is needed for farmers. To test the equipment appropriately, we prepare the samples and antibodies for rapid detecting the Lawsonia intracellularis in pig feces. Methods : To prepare the PPE infected samples, we sampled PPE suspected pig feces in a pig farm. To manufacture a anti-Lawsonia intracellularis antibody for capturing the Lawsonia intracellularis, the rabbit-anti LsaA synthetic peptide polyclonal antibody was inoculated to rabbits. To select the couple of antibodies which is most well sandwiched with the bacteria, ELISA test was done with PPE infected ileum samples. Finally, to verify the PPE infected feces which would be used to test the rapid kit, PCR test was done on the sampled PPE suspected feces Results : The rabbit-anti LsaA synthetic peptide polyclonal antibody is developed, and is verified to capture the bacterial well through the fluorescence antibody test. Also, we found that the monoclonal antibody and the polyclonal antibody could be used as couples for sandwiching the bacteria. Finally, through the PCR test for samples of pig feces, we could prepare the 150 PPE positive samples and 50 PPE negative samples. Conclusions : The manufactured polyclonal antibody and the imported monoclonal antibody could be used to capture the bacteria using the sandwich techniques. Also, the prepared PPE infected negative and positive samples could be used to test the performance of the rapid kit to capture the bacterium Lawsonia intracellularis.

Exploring the Microbial Community and Functional Characteristics of the Livestock Feces Using the Whole Metagenome Shotgun Sequencing

  • Hyeri Kim;Eun Sol Kim;Jin Ho Cho;Minho Song;Jae Hyoung Cho;Sheena Kim;Gi Beom Keum;Jinok Kwak;Hyunok Doo;Sriniwas Pandey;Seung-Hwan Park;Ju Huck Lee;Hyunjung Jung;Tai Young Hur;Jae-Kyung Kim;Kwang Kyo Oh;Hyeun Bum Kim;Ju-Hoon Lee
    • Journal of Microbiology and Biotechnology
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    • 제33권1호
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    • pp.51-60
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    • 2023
  • The foodborne illness is the important public health concerns, and the livestock feces are known to be one of the major reservoirs of foodborne pathogens. Also, it was reported that 45.5% of foodborne illness outbreaks have been associated with the animal products contaminated with the livestock feces. In addition, it has been known that the persistence of a pathogens depends on many potential virulent factors including the various virulent genes. Therefore, the first step to understanding the public health risk of livestock feces is to identify and describe microbial communities and potential virulent genes that contribute to bacterial pathogenicity. We used the whole metagenome shotgun sequencing to evaluate the prevalence of foodborne pathogens and to characterize the virulence associated genes in pig and chicken feces. Our data showed that the relative abundance of potential foodborne pathogens, such as Bacillus cereus was higher in chickens than pigs at the species level while the relative abundance of foodborne pathogens including Campylobacter coli was only detected in pigs. Also, the microbial functional characteristics of livestock feces revealed that the gene families related to "Biofilm formation and quorum sensing" were highly enriched in pigs than chicken. Moreover, the variety of gene families associated with "Resistance to antibiotics and toxic compounds" were detected in both animals. These results will help us to prepare the scientific action plans to improve awareness and understanding of the public health risks of livestock feces.

경북 북부지역 재래산양 분변에서의 기생충 감염률 조사 (Infection rate of parasites from feces of Korean indigenous goats in northern areas of Gyeongbuk province)

  • 조광현;이지홍;김대중;김승준;권오덕;곽동미
    • 한국동물위생학회지
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    • 제31권3호
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    • pp.357-362
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    • 2008
  • While studies have been carried out on endoparasite infection from feces of Korean indigenous goats in multiple areas around the nation, there is no report in northern areas of Gyeongbuk province. Thus, this study was conducted to examine the rate of parasite infection from the fecal samples of goats raised on grass. From March to July in 2008, 108 fecal samples were taken from goats. Eggs of parasites were identified using flotation or sedimentation methods followed by microscopic examination. Overall infection rate of parasites from feces of goats were 99% (107 out of 108). The infection rates of nematode, cestode, trematode and protozoa were 37%, 23%, 0% and 99%, respectively. Among parasite eggs detected, nematodes included threadworms (20%), strongyles (16%) and whipworms (1%). The only cestode and protozoa detected were Moniezia expansa and Eimeria spp, respectively. In the rates of mixed infection, single was 61%, double 32%, and triple 6%.

오리 분변에서의 Campylobacter jejuni 오염도와 항생제 내성유형 조사 (Prevalence and antimicrobial resistance patterns of Campylobacter jejuni from duck feces)

  • 김능희;채희선;강영일;신방우;최농훈;김효비
    • 한국동물위생학회지
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    • 제36권1호
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    • pp.57-60
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    • 2013
  • This study was carried out to investigate the prevalence and antimicrobial resistance patterns of Campylobacter jejuni isolated from duck feces. In total, 112 (32.9%) isolates of C. jejuni were identified from 430 duck feces samples from September to December, 2010. All isolates were susceptible to telithromycin, whereas majority of the C. jejuni isolates were resistant to azithromycin (18.8%), ciprofloxacin (86.6%), erythromycin (0.9%), gentamicin (15.2%), tetracycline (80.4%), florfenicol (3.6%), nalidixic acid (87.5%), clindamycin (7.1%). As a result, appropriate protocols for antimicrobial agents and strategies to reduce antimicrobial resistance will be needed in the future.

Mineral Compositions in the Feces of Some Silkworms

  • Kim, Iksoo;Kang, Pil-Don;Jang, Sung-Kee;Ryu, Kang-Sun;Kim, Doh-Hoon
    • International Journal of Industrial Entomology and Biomaterials
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    • 제7권1호
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    • pp.75-78
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    • 2003
  • This study was carried out to investigate the mineral content of the feces of the $5^th$ instar larvae in a few silkworm species such as a parent domestic silkworm (Jam134), a hybrid (Kumok-jam), the Japanese oak silkworm, and the Chinese oak silkworm. The major minerals of all silkworms throughout all $5^th$ instar larval period are K, P, Mg and Ca, and the result is consistent with the previous study of the mineral content in the mulberry leaves. Although the calcium content decreased sharply at $7^th$ day of the $5^th$ instar, the crude protein content significantly increased at the same age in both domestic silkworms, suggesting a direct relationship between feeding behavior of the silkworms at the larval period and mineral/protein contents. However, this trend was not observed in both oak silkworms. In the comparison of the mineral content among silkworm species, two domestic silkworms were significantly higher in the calcium content compared with two oak silkworms, and the hybrid Kumok-jam was further higher significantly than the feces of parental Jam 143. Excluding the calcium content, overall no significant content variation in other minerals was observed among four silkworm species studied in this study.