Browse > Article

Use of Genetic Techniques to Analyze Wintering Population of Geese in Korea with Noninvasive Feces Samples  

Kim, Min-Kyung (Department of Environmental Science and Engineering, College of Engineering, Ewha Womans University)
Won, Yong-Jin (Division of EcoScience, Ewha Womans University)
Lee, Sang-Don (Department of Environmental Science and Engineering, College of Engineering, Ewha Womans University)
Publication Information
Korean Journal of Environmental Biology / v.26, no.3, 2008 , pp. 264-269 More about this Journal
Abstract
This study was intended to test the feasibility of genetic analysis of wintering population of geese using their feces samples. This noninvasive approach is quite significant and effective because we do not need to capture or harm geese to obtain the samples. We collected the feces from two different populations of wintering geese in Korea in 2007. Finally thirty two feces were analyzed through a molecular genetic method. As a result, 14 haplotypes were identified and classified into two groups, white-fronted geese (Anser albifrons) and bean geese (Anser fabalis). We established the method to make molecular genetic experiment more efficient using the feces. This study has a significance as the first genetic result on wintering population of geese in Korea using noninvasive sampling method.
Keywords
geese; feces; natural monument; molecular method; noninvasive sampling;
Citations & Related Records
연도 인용수 순위
  • Reference
1 김인규, 김창숙, 함규황. 1999. 최근 10년 ('89-'98)간 주남 저수지 조류의 종수 및 개체수 변동에 관한 연구. 한국조류학회지. 6:127-132
2 박진영, 원병오. 1993. 주남저수지에 도래하는 큰기러기와 쇠기러기의 월동생태. 경희대학교 한국조류연구소. 4:1-24
3 Cottrell PE. AW Trites and EH Miller. 1996. Assessing the use of hard parts in faeces to identify harbour seal prey: results of captive-feeding trials. Can. J. Zool. 74:875-880   DOI   ScienceOn
4 Deagle BE. DJ Tollit. SN Jarman. MA Hindell. AW Trites and NJ Gales. 2005. Molecular scatology as a tool to study diet: analysis of prey DNA in seatS from captive Steller sea lions. Mol. Ecol. 14:1831-1842   DOI   ScienceOn
5 Taberlet P. JJ Cammara. S Griffin. E Uhres. O Hanotte. LP Waits. C Dubois-Paganon. T Burke and J Bouvet. 1997. Non-invasive genetic tracking of the endangered Pyrenean brown bear population. Mol. Ecol. 6:869-876   DOI   ScienceOn
6 Paxinos E. C Mcintosh. K Ralls and R Fleischer. 1997. A noninvasive method for distinguishing among canid species: amplification and enzyme restriction of DNA from dung. Mol. Ecol. 6:483-486   DOI   ScienceOn
7 문화재정보센터(http://www.cha.go.kr/newinfo/Culresult_Db_View.jsp?VdkVgwKey=16,03250000,ZZ&queryText=V_KDCD=16)
8 Trites AW and R Joy. 2005. Dietary analysis from fecal samples: how many scats are enough? J. Mammal. 86:704-712   DOI   ScienceOn
9 Hamilton MJ. T Yan and MJ Sadowsky. 2006. Development of goose- and duck-specific markers to determine source of Excherichia coli in waterways. Appl. Environ. Microb. 72:4012-4019   DOI   ScienceOn
10 Horvath MB. B Martinez-Cruz. JJ Negro. L Kalmar and JA Godoy. 2005. An overlooked DNA source for non-invasive genetic analysis in birds. J. Avian biol. 36:84-88   DOI   ScienceOn
11 Lampa S. B Gruber. K Henle and M Hoehn. 2008. An optimisation aproach to increase DNA amplification success of otter faeces. Conserv. Genet. 9:201-210   DOI
12 Kurose N. R Masuda and M Tatara. 2005. Fecal DNA analysis for identifying species and sex of sympatric camivores: A noninvasive method for conservation un the Tsushima islands. Japan. J. Hered. 96(6):688-697   DOI   ScienceOn
13 Chambers PA. PS Duggan. JM Forbes and J Heritage. 2001. Arapid. reliable method for the extraction from avian faeces of total bacterial DNA to be used as a template for the detection of antibiotic resistance genes. J. Antimicrob. Chemoth,47:239-246   DOI   ScienceOn
14 Segelbacher G. 2002. Noninvasive genetic analysis in birds: testing reliability of feather samples. Mol. Ecol. 2:367-369   DOI   ScienceOn
15 Reed JZ. DJ Tollit. PM Thompson and W Amos. 1997. Mole- cular SCatology :the use of molecular genetic analysis to assign species. sex and individual identity 10 seal faeces. Mol. Ecol. 6:225-234   DOI   ScienceOn
16 Sorenson MD. JC Ast, ED Dimcheff. T Yuri and DP Mindell. 1999. Primers for a PCR-based approach to mitochondrial genome sequencing in birds and other vertebrtes. Mol. Phylogenet. Evol. 12:105-144   DOI   ScienceOn
17 Idaghdour Y. D Broderick and A Korrida. 2003. Faeces as a source of DNA for molecular studies in a threatened population of great bustards. Conserv. Genet. 4:789-792   DOI