• Journal of Animal Science and Technology
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• v.63 no.1
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• pp.125-136
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• 2021

Bovine coronavirus (BCoV) is associated with severe diarrhea, dehydration, and depression, which result in significant economic damages in the dairy and beef cattle industries worldwide. However, differences in the gut microbiota structure and their correlations with differing physiological parameters between BCoV-infected calves with diarrhea and recovered calves are not well understood. In this study, fecal specimens were collected from 10 post-weaned calves, before and after 2 months of fluid therapy, and the samples were used for microbiota analysis. Following recovery, the alpha-diversity profiles (observed operational taxonomic units [OTUs], and Chao1, Shannon, and Simpson indices) changed significantly when compared with those of calves with diarrhea. Beta-diversity analysis exhibited significant differences in gut microbiota compositions between calves with diarrhea and those in the recovered state. The abundances of eight phyla and thirteen genera in feces changed markedly after restoration of BCoV diarrhea. In addition, our correlation study clearly revealed that increased abundances of the genera Caproiciproducens, Pseudoflavonifractor, and Oscillibacter negatively correlated with serum glucose, and phosphorus levels, but positively correlated with serum chloride in calves with diarrhea, whereas increased abundances of the genera Peptostreptococcaceae;Clostridium (Clostridium cluster XI), Intestinibacter, Cellulosilyticum, Ruminococcus, Romboutsia, Paeniclostridium, Clostridiaceae;Clostridium and Turicibacter in recovered calves showed the opposite pattern. These results suggest that structural changes of the gut microbiota after recovery from BCoV infection correlate with changes in physiological parameters. In conclusion, our data provide evidence of gut microbiota-composition changes and their correlations with the physical profiles of post-weaned calves, before and after fluid therapy for BCoV-related diarrhea.

• Food Science and Biotechnology
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• v.16 no.4
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• pp.605-609
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• 2007

The effects of lactic acid fermented yam yogurt (Yam/YG) on colon mucosal tissue were investigated in a loperamide-induced constipation rat models. Sprague-Dawley rats were fed for 6 weeks with 3 types of diets (normal, supplemented with lactic acid bacteria, and supplemented with Yam/YG), and were then administered loperamide intraperitoneally twice daily for 5 days. Administration of loperamide decreased fecal excretion and the moisture content of feces with increasing of numbers of pellets in the colon. On the histopathologic findings from hematoxylin and eosin (H& E) and alcian blue stainings, supplementation with Yam/YG resulted in the recovery of depleted goblet cells and mucin, and increased the numbers of Ki-67 positive cells, indicating restoration of colonic mucosa through cell proliferation and crypt regeneration against damages observed in crypt epithelial cells of loperamide-induced rats. These results indicate that Yam/YG improves evacuation and mucus production in the gastrointestinal tracts of constipated-induced rats.

• Journal of Veterinary Clinics
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• v.17 no.2
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• pp.459-463
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• 2000

A 3 year-old male American Pit Bull Terrier in Kwang-Ju, Chonnam was admitted to the Teaching Animal Hospital, Chonbuk National University. He showed convulsive spasms of skeletal muscles, raised tail-head, and rigidity of rigidity. Based on these clinical sign\ulcorner tetanus was suspected Hematological test results showed and WBC value (21 ,800/$\mu$l/) with neutrophils (17.877/$\mu$l) and monocytes (1.744/$\mu$l). The blood chemical values ALT (86 IU/L) and AST (119 IU/L) were elevated. Fecal sample from the dog was cultured in anaerobic chamber. A Gram positive, drumstick shaped bacteria was isolated. The bacteria was identified as Clostridium tetani by biological and biochemical tests. The dog was hospitalized in a quiet place where the light was cut off and treated with penicillin G procaine and phenobarbital sodium. The dog made a complete recovery 25 days after treatment. This Is the first report on C. tetani infection of domestic dog in Korea.

• Parasites, Hosts and Diseases
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• v.37 no.1
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• pp.13-19
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• 1999

The effects of in vitro culture methods on morphological development and infectivity of Strongyloides venezuelensis filariform larvae ($L_3$) to rats were investigated. A significantly higher body length was observed in $L_3$ from filter paper culture ($597.3{\;}{\pm}{\;}32.2{\;}{\mu\textrm{m}}$) than those in fecal (($509.9{\;}{\pm}{\;}35.0{\;}{\mu\textrm{m}}$) and nutrient broth culture (503.3{\;}{\pm}{\;}31.0{\;}{\mu\textrm{m}}) (P<0.05). Larval infectivity was assessed by exposing rats to 1,000 $L_3$ from each culture and worms were recovered from the lungs and small intestines. Recovery rate of these worms did not show any significant difference. A significantly greater body length of adults was recorded in those corresponding to the $L_3$ harvested from filter paper (2,777.5{\;}{\pm}{\;}204.4{\;}{\mu\textrm{m}}$) and nutrient broth culture (($2.732.5{\;}{\pm}{\;}169.8{\;}{\mu\textrm{m}}$) than those corresponding to the $L_3$ obtained from fecal culture (($2.600.5{\;}{\pm}{\;}172.4{\;}{\mu\textrm{m}}$) (P<0.05). Although worm fecundity and EPG counts differed among culture methods but worm burdens and course of infection did not. These findings suggest that the methods of cultures have a significant effect on the morphological development of the larvae to the $L_3$ stage, but do not influence the infectivity to rats.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.2 no.3
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• pp.170-179
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• 2021

Hulunbuir steppe, one of the four largest steppes in China, has experienced rapidly progressing desertification partly due to overgrazing by livestock. The objective of this study was to investigate the effectiveness of various vegetation recovery methods, including the selection of unpalatable plants less affected by grazing livestock. To determine livestock grazing preferences at Hulunbuir restoration sites, we used DNA barcoding methods to analyze fecal materials of horses and cattle grazing on four restored plants: two trees (Pinus sylvestris L. var. mongolica and Populus canadensis) and two shrubs (Caragana microphylla and Corethrodendron fruticosum). Neither of the two tree species were detected in livestock feces, whereas both shrub species were detected at lo w frequencies. There were no significant differences in compositions of species consumed by horses and cattle except that Asteraceae species were more often consumed by cattle. Our results showed that the four plants used for restoration may be classified as unpalatable or less palatable species in the Hulunbuir restoration area. Our results may help inform restoration strategies implemented in restoration areas, especially regarding negative effects of livestock grazing during the initial stage of restoration in Hulunbuir.

• Journal of Environmental Health Sciences
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• v.46 no.6
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• pp.646-654
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• 2020

Objectives: Users of parks or children's play facilities have pointed to pets' bowel movements as the most serious problem when using them. In prior studies, a very low detection rate of parasites (eggs) in sand flooring materials has been found. Even though feces have been identified, no parasites (eggs) have been detected. Method: A standard solution of nitrate nitrogen was used to verify the reliability of a new nitrogen analysis method. The linearity, precision, and accuracy of the nitrate nitrogen analysis method were verified. Using this method, the pollution distribution of the sand flooring material and the degree of pollution at each point were investigated. Results: As a result of the verification of the nitrogen analysis method, the linearity was found to be good at r2=0.999 when distilled water is mixed in a standard substance solution. The standard substance additive solution r2=0.968 was found to be good. Precision represented 0.01 to 0.06% RSD for peak height. The recovery rate was 92.4 to 104.0 percent, indicating high accuracy. According to the same method of analysis, the flooring material sand at a general amusement facility with the largest number of concealed spaces was nitrate nitrogen 6.1 times higher than at the entrance of the playground. Also, in a comparison between clean sand and sandy flooring, the average nitrogen concentration of the sand flooring material was 24.4-167 times higher than pure sand. Conclusions: As such, no parasites (eggs) were detected at all points under investigation, but the sand flooring was exposed to animal fecal contamination. Therefore, the management of nitrogenous components should allow accurate identification of animal fecal contamination so that the timing of sand replacement can be managed hygienically and safely.

• Korean Journal of Food Science and Technology
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• v.52 no.5
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• pp.482-486
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• 2020

Cases of inflammatory bowel diseases including ulcerative colitis are increasing in Korea and development of non-steroidal anti-inflammatory drugs (NSAIDs) are widely investigated. Natural products with anti-inflammatory properties are rising as safe candidates for NSAIDs. The extract of turmeric or puffed turmeric mixed with herbal extract (goji berry, liquorice, lycium root, and dong quai) was treated to acute colitis mice by oral gavage. The symptoms of colitis, i.e., body weight loss, fecal score, and shortened colon length, were significantly attenuated by puffed turmeric extract with the herbal extract. Non-puffed turmeric extract with herbal extract, however, exhibited a very marginal recovery. Tissue culture supernatant of colons further revealed that both puffed turmeric and non-puffed turmeric extracts with herbal extract suppressed pro-inflammatory cytokine production at a comparable level. These results indicate that puffing is a simple and promising process of turmeric for enhancement of anti-inflammatory properties.

• The Journal of Internal Korean Medicine
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• v.22 no.1
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• pp.113-118
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• 2001

In July, 2000, A 71-year-old man complained of these symptoms: frequent defecation, urgent need to defecate, fecal leakage, anal pain, flatus, and diarrhea after surgery for colorectal cancer. The symptoms are caused specifically by injury to the bowel and sphincter function. The patient suffered physically and psychologically; but, there was no special treatment in western medicine. Therefore, we treated him with electrical acupuncture for the management of sphincter tone and the alleviation of anal pain. We also treated him with herbal medicine for the recovery of strength and gastro-colic function. As a consequence of these treatments, the bowel disorders improved immediately and successfully, even though there were some limitations. So, We report this case with a brief review of related literatures.

• Journal of Life Science
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• v.10 no.4
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• pp.368-373
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• 2000

Enteroviruses in the environment pose a public health risk because they can be transmitted via the fecal-oral route through contaminated water, and low numbers are able to initiate an infection in humans. Because the levels of viruses typically found in environmental water and drinking water are low, they must be concentrated from hundreds to thousands of liters of water. Therefore, the main goal of this study was the development of a rapid, simple and efficient procedure to concentrate, isolate and detect enteroviruses from environmental water samples. Viruses were first concentrated by adsorption to 1 MDS cartridge filter and then eluted with approximately 0.5 liter of 1.5% beef extract/0.05M glycin(pH 9.4). In this study, several procedures to concentrate and purify intact viruses from beef extract obtained from the adsorbent filters were tested. Among them, organic floccuration was the best reliable method for reconcentration. sample volume could be reduced to 200∼400 folds and the efficiency of virus recovery through the procedure was over 72%. Finally, the samples were filtered through a membrane disk filter and then analyzed by either the plaque assay or combined cell culture-polymerase chain reaction.

• Asian-Australasian Journal of Animal Sciences
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• v.26 no.1
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• pp.65-71
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• 2013

Two digestion trials, one with sheep and another with goats, were conducted to evaluate the long-term bias (LTB) of the indigestible dry matter (iDM), indigestible neutral detergent fiber (iNDF) and indigestible acid detergent fiber (iADF) internal markers. The study used eight Santa In$\hat{e}$s castrated male sheep (average body weight of 16.6 kg) distributed in two $4{\times}4$ Latin squares and eight Saanen castrated male goats (average body weight of 22.6 kg) distributed in two $4{\times}4$ Latin squares. The experiments were conducted simultaneously, and the animals were housed in 1.2 $m^2$ individual pens with wood-battened floors equipped with individual feeders and drinkers. The animals received isonitrogenous diets that were offered ad libitum and contained 14% crude protein and 70% sugar cane (with 0, 0.75, 1.5 or 2.25% CaO, in natural matter percentage), corrected with 1% urea and 30% concentrate. The experiment consisted of four experimental periods of 14 d each, with the feed, leftovers and feces sampled on the last four days of each period. The marker concentrations in the feed, leftovers and fecal samples were estimated by an in situ ruminal incubation procedure with a duration 240 h. The relationship between the intake and excretion of the markers was obtained by adjusting a simple linear regression model, independently from the treatment (diets) fixed effects and Latin squares. For both the sheep and goats, a complete recovery of the iDM and iNDF markers was observed (p>0.05), indicating the absence of LTB for these markers. However, the iADF was not completely recovered, exhibiting an LTB of -9.12% (p<0.05) in the sheep evaluation and -3.02% (p<0.05) in the goat evaluation.