• Journal of Plant Biotechnology
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• v.40 no.2
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• pp.65-71
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• 2013

Lilium cernum Komarvo. is an important endangered plant belonging to the family Liliaceae. A method was developed for the rapid micropropagation of L. cernum through plant regeneration from bulb scales explant-derived calli. The bulb scales segments were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1.0, $3.0mg{\cdot}L^{-1}$ kinetin and 0, 0.1, 0.5, $1.0mg{\cdot}L^{-1}$ NAA or 2,4-D for callus induction. In media with $0.5{\sim}3.0mg{\cdot}L^{-1}$ kinetin and $0.1{\sim}1.0mg{\cdot}L^{-1}$ NAA and 2,4-D, 95~100% of explants produced callus. They were then transferred to MS medium supplemented with various concentrations of NAA (0, 0.01, 0.05 and $1.0mg{\cdot}L^{-1}$) in combination with BA (0, 1.0 and $2.0mg{\cdot}L^{-1}$) for bulbet formation. Bulbet induction (78%), weight (468 mg) and size (15.5 mm) were obtained the highest on MS medium containing $2.0mg{\cdot}L^{-1}$ BA and $1.0mg{\cdot}L^{-1}$ NAA. In vitro frequency of plant regeneration was not significantly treated in strength of MS and sucrose concentration. Chlorophyll contents in 1/2MS with $50g{\cdot}L^{-1}$ sucrose treatments were higher than those in control and another treatment. This in vitro propagation protocol will be useful for conservation and mass propagation of this endangered plant.

• Journal of Life Science
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• v.21 no.2
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• pp.221-226
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• 2011

The gene encoding 4-$\alpha$-glucanotransferase (mgtA) from Thermotoga neapolitana was cloned and expressed in Escherichia coli in order to investigate whether this enzyme was capable of producing cycloamylose for industrial applications. MgtA was purified to homogeneity by HiTrap Q HP and Sephacryl S-200 HR column chromatographies. The size of the enzyme as determined by SDS-PAGE was about 52 kDa, which was in good agreement with its deduced molecular mass of 51.9 kDa. The optimal temperature and pH for the activity of the 4-$\alpha$-glucanotransferase was found to be $85^{\circ}C$ and 6.5, respectively. The enzyme hydrolyzed the 1,4-$\alpha$-glucosidic bonds in oligomeric 1,4-$\alpha$-glucans and transferred oligosaccharides (maltotriose being the shortest one) to acceptor maltodextrins. However, the enzymes had no activity against pullulan, glycogen, and other di- or trioligosaccharides with rare types of $\alpha$-bond. MgtA is distinguished from 4-$\alpha$-glucanotransferase from Thermotoga maritima in that it can convert maltotriose into maltooligosaccharides. The treatment of glucoamylase after the reaction of MgtA with maltotriose, maltotetraose, maltopentaose, or maltohexaose as sole substrate revealed that MgtA yielded linear maltooligosaccharides instead of cycloamylose.

• Journal of Genetic Medicine
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• v.4 no.1
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• pp.84-87
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• 2007

Spinocerebellar Ataxia Type 3 (SCA 3) is a rare autosomal dominative disorder in which one of the neurodegenerative disorders is caused by a CAG repeat expansion on chromosome 14q32.1. The age at onset of disease is related to the size of the expanded CAG repeat. We present the prenatal diagnosis of SCA3 in a woman whose husband was known to carry an unstable CAG repeat expansion in the MJD gene. The diagnosis was made using PCR with a fluorescent probe for an expanded MJD allele. The normal ranges of (CAG)n of SCA3 are 14~38 repeats. The husband, who had a family history of SCA 3, has an expanded allele of 69 CAG repeats with a normal allele of 27 repeats. His wife had two normal alleles with 26 and 32 CAG repeats. The fetus had two normal alleles with 26 and 27 CAG repeats; consequently, the baby w as healthy. We report a case of prenatal diagnosis of SCA3 using a fluorescent PCR which is rapid and accurate.

• Journal of the Korean Society of Food Culture
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• v.15 no.2
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• pp.147-153
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• 2000

A survey on the preferences and notion about kimchi and kimchi products was conducted from july to october, 1999 to investigate basic information for increasing the consumption of kimchi for sale. 1,241 subjects of women and men aged 10 to 60 in Kyung-nam area participated in this survey. Most of the subjects made kimchi by themselves at home but this rate of making kimchi at home became lower than past, so increment of consumption of kimchi for sale will be expected in the future. When the people buy kimchi, they considered taste first, and then hygiene, nutrition in order. When they purchase kimchi, more men (69.1%) than women(56%) considered taste, but more women(36.1%) than men(20.6%) considered hygiene first. Subjects aged 30 or older groups considered more the hygiene of kimchi than the subjects of $10{\sim}20$ age groups did.(p<0.05) And people of household income over 3 million wons considered more hygiene than people of below 1 million wons income group did. People liked manufactured kimchi the packed with whole or partly transparent material in order to observe the contents.(p<0.05) They liked better kimchi packed with bottle(46.1%) and vinyl(39.6%) than plastic(14.3%) and this tendency was more in the subjects of women(49.8%) and over 30 age groups.(p<0.05) They prefered $200{\sim}500g$ packing unit of kimchi whether the family size were big or small. Among the processed kimchi products, men liked better a rice covered up with kimchi, a pot stew with kimchi or a soup with kimchi, On the other hand, women liked better a dumplings with kimchi, a grilled food with kimchi, a pizza with kimchi.(p<0.05) Subjects aged 10 to 20 years old liked all kinds of kimchi products, but people aged 40 or older disliked them.(p<0.001)

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.10
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• pp.527-536
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• 2016

The purpose of this study was to explore the components of the geriatric robot pet, which might be helpful in caring for elderly people in the future. Focus group interview-with two focus groups-was performed between August 2014 and December 2014. Study participants were 13 geriatric experts from nursing, medicine, social work, and physical/occupational therapy. All interview data were transcribed, followed by debriefing and a qualitative content analysis. Three main themes emerged: acknowledgment of the usefulness of geriatric robot pet, establishment of elderly-centered appearance, and the functions of the robot pet to meet the needs of elders and family members. The robot pet was considered to be useful to elders who live alone since they can benefit from companionship and care provided by the robot pet. Geriatric robot pet needs to be manageable in size and weight, like a small dog. Moreover, a function that allows for interaction with and monitoring the elderly would be beneficial. The baseline elements that could guide further development of contents for the Korean geriatric robot pet were proposed from the results of FGI. Studies that validate these elements are necessary.

• Korean Journal of Social Welfare Studies
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• v.40 no.3
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• pp.327-348
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• 2009

The purpose of this study is to understand the diverse lifestyles of Korean older adults by analyzing the consumption pattern of older households and its determinants. The 9th wave of the Korea Labor and Income Panel Study(KLIPS) data was used for analysis. The twenty consumption items provided by the dataset was reduced to thirteen according to the consumption purpose inherent in the item. K-means cluster analysis and multinomial logistic regression was employed to categorize the consumption pattern of older households and to analyze the determinants. The results are as follows. The consumption pattern of Korean older adults was clustered into six distinctive groups named Breadwinner, Leisure-time pursuer, Friendly outgoes, Daily-life survivor, Illness sufferer and Shelter seeker. Breadwinner, Leisure-time pursuer and Friendly outgoes were lifestyles that earn and spend more compared to the other three. Nevertheless, they differed according to the family size, indicating that the parenting burden might have direct influence on the lifestyle of Korean older adults. Older adults without parenting burden and with high level of education and economic capacity were likely to show Friendly outgoes lifestyle. On the other hand, Daily-life survivor, Illness sufferer and Shelter seeker showed lower level of spending, indicating that for those lacking in economic capacity, urgent needs such as medical need or housing need dominates the lifestyle. The results call for adequate custom policies that best fit the needs of older adults.

• Korean Journal of Plant Resources
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• v.34 no.4
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• pp.270-277
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• 2021

Veronica rotunda var. subintegra (Nakai) T. Yamaz. is perennial herb belonging to the family Scrophulariaceae, native to Korea. Plant growth regulators, cutting mediums, cutting position, and leaf size were examined to investigate the requirements for cutting propagation of V. rotunda var. subintegra. This study was conducted over a period of 6 weeks from March to May 2017 in the Division of Plant Resources, Korea National Arboretum. For all treatments, survival and rooting percentage was 100%. In PGRs experiment, the number of roots and shoot length treated with 100 mg/L IBA treatment was significantly higher than that of NAA treatment. The shooting percentage was decreased with an increase of the PGRs concentration. In growing substrates experiment, the number of roots on commercial soil (61.2) was the highest, followed commercial + kanuma soil (45.7), and the lowest was kanuma soil (41.8). The shoot length treated with commercial soil (8.9 cm) was significantly greater than that of kanuma soil (4.1 cm). In cutting positions experiment, the number of roots treated with apical (56.6) was significantly higher than that of basal (38.8). In leaf-cut experiment, the number of roots and of shoot length treated with full leaf was significantly higher than that of half leaf. As a result, we recommend treating cuttings with 100 mg/L IBA treatment, commercial soil, apical position, and full leaf for successful cutting propagation of V. rotunda var. subintegra.

• Journal of Life Science
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• v.28 no.11
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• pp.1332-1338
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• 2018

Activated microglia, induced by various pathogens, protect neurons and maintain homeostasis of the central nervous system (CNS). However, severe activation causes neurodegenerative disorders such as Alzheimer's disease and Parkinson's disease because of the secretion of various neurotoxic molecules, such as nitric oxide (NO), prostaglandin (PG), and pro-inflammatory cytokines. Because chronic microglial activation endangers neuronal survival, negative regulators of microglial activation have been identified as potential therapeutic candidates for treatment of many neurological diseases. One potential source of these regulators is Locusta migratoria, a grasshopper of the Acrididae, usually 4-6 cm in size, belonging to the family of large insects in Acrididae. This grasshopper is an edible insect resource that can be consumed by humans as protein source or used for animal feed. The aim of the present study was to examine the inhibitory effects of a L. migratoria ethanol extract (LME) on the production of inflammatory mediators in LPS-stimulated BV-2 microglia cells. The extract significantly inhibited the NO, iNOS, COX-2, and pro-inflammatory cytokine ($TNF-{\alpha}$, IL-6 and $IL-1{\beta}$) levels in BV-2 microglia cell. Because the inhibition of microglial activation may be an effective solution for treating brain disorders like Alzheimer's and Parkinson's diseases, these results suggest that LME may be a potential therapeutic agent for the treatment of brain disorders induced by neuroinflammation.

• Journal of Convergence for Information Technology
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• v.9 no.3
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• pp.112-119
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• 2019

This study examined the role of citizen advocates in advocating for people with developmental disabilities. Citizen advocacy refers to activities that advocate and represent citizens on behalf of those who are socially vulnerable. The purpose of this study is to analyze the role concept of citizen advocates by conducting focus group interviews and dividing the 13 citizen advocates with developmental disabilities into 4 groups. It is divided into four main subjects and nine sub-themes. The main theme are 'Self-transformation of the parties', 'Restoring the rights of the parties', 'Being with the parties', and 'Expanding the world of the parties'. Sub-themes are 'Recognizing and solving the problem of self', 'Granting role to speak', 'Recovering the initiative of service use', 'Restoring the rights of existing (family and neighbor), "Preventing money harm" "Speaking instead", "Encouraging and petting people", "Promoting change in the community", and "Expanding the size of the world a little bit". Based on this, we discussed the importance of citizen advocacy as a preventive advocacy system, the relationship between citizen advocacy and professional advocacy system, the difference between volunteer and personal assistants and citizen advocacy, and considerations for citizen advocacy.

• The Journal of the Korea Contents Association
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• v.18 no.12
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• pp.140-149
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• 2018

Along with the recent increase in national income, social phenomena such as aging due to a decrease in population and an increase in single households are observed. There are also an increasing number of households raising pets in proportion to aging households and the increase in the number of single households, most of which use animal companions to overcome loneliness and boost domestic vitality. As more and more people consider pets as family members, the size of the domestic pet market is also growing. The growing number of pets in older households and single households is not properly managed by care such as food meals and exercise management for pets. It is necessary to research and develop robots that can monitor animal companions remotely, feed a certain amount of food at regular intervals, and manage their health through exercise. Among pet companions, dog selection is the highest. Therefore, this study identified robot research on driving methods, examples of existing pet care systems, and researched pet care robots using obstacle avoidance algorithms. In order to use the snack pay behavior and obstacle avoidance algorithm of the pet animals by applying IoT and we .oPI technology, it is able to use ultrasonic sensors on the front and has four infrared sensors on the back. However, this study does not reflect the characteristics of other pet animals as a study on pet care robots, and it requires continuous observation and testing.