• Korean Journal of Plant Resources
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• v.16 no.3
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• pp.238-244
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• 2003

During the life cycle, plants have to suffer from various environmental stresses. A common element in response to many environmental stresses is cellular dehydration. Dehydrins are a family of proteins commonly induced by environmental stresses associated with low temperature or dehydration and during seed maturation drying. For the study in the defense mechanism against various stresses, a cDNA clone encoding a dehydrin gene was isolated from a cDNA library prepared from tab root mRNAs of Codonopsis lanceolata. The cDNA, designated ClDhn1, is 893 nucleotides long and has an open reading frame of 480 bp with a deduced amino acid sequence of 159 residues. The ClDhn1 amino acid sequence is highly hydrophilic and possesses two conserved repeats of characterized lysine­rich K­segment (KIKEKLPG), and a 7­serine residue stretch prior to the first lysine­rich repeat that is common to many dehydrins. The DEYGNP conserved motif is, however, modified in the sequence of ClDhn1 gene. The deduced amino acid sequence of ClDhn1 was compared with other plant dehydrinls and showed high homology with Solanum commersonii

• Korean Journal of Plant Resources
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• v.27 no.1
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• pp.95-101
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• 2014

This study was conducted to analyze the pattern of growth and phenological characteristics of Mankyua chejuense B.-Y. Sun, M.H. Kim & C.H. Kim, which belong to the Ophioglossaceae family. M. chejuense asexually reproduced using rhizomes, and a clone of the species grew up to 52 cm based on root growth and new leaves was produced from rhizomes. The development of leaves were divided into four stages; leaf emergence-separation and growth of leaf-sporophyll maturation-senescence. Most leaves emerged in July and August with high temperatures and precipitation and most leaves reached full expansion during September-October and died during April-May next year. The life span of leave was 10 months, from July to April, and the start of leaf senescence varied depending on the habitat environment and this might result from micro environmental differences among the habitats.

• Proceedings of the Korean Institute Of Construction Engineering and Management
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• 2007.11a
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• pp.353-357
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• 2007

The Apartment resident's make-up of a family and residing preference are diversified by the change of lifestyle through the elevation of economy level and residing level. Accordingly, the remodeling and the early rebuilding for the satisfaction of a life cycle and a life style are increasing, and "Variability" that is the concept of Open Housing was emphasized as a central of residing plan in the apartment. Although apartments which select the space variability to the residing space are increasing gradually, the optimum composition structural system with space variability that satisfies the structure stability, the economical efficiency and the resident usability is not developed. The method of a study was progressed by a comparison and an analysis on each system with estimation elements through analyzing the development process and the special quality of existing composition structural system with space variability in the apartment. This paper offered the improvement direction on the current composition structural system with space variability for the development of modularizing system that is correct in domestic real condition.

• Reproductive and Developmental Biology
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• v.42 no.2
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• pp.7-12
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• 2018

In this review, we have tried to summarize the evidence and molecular characterization indicating that $20{\alpha}$-hydroxysteroid dehydrogenase ($20{\alpha}$-HSD) is a group of the aldo-keto reductase (AKR) family, and it plays roles in the modulation and regulation of steroid hormones. This enzyme plays a critical role in the regulation of luteal function in female mammals. We have studied the molecular expression and regulation of $20{\alpha}$-HSD in cows, pigs, deer, and monkeys. The specific antibody against bovine $20{\alpha}$-HSD was generated in a rabbit immunized with the purified recombinant protein. The mRNA expression levels increased gradually throughout the estrous cycle, the highest being in the corpus luteum (CL) 1 stage. The mRNA was also specifically detected in the placental and ovarian tissues during pregnancy. The $20{\alpha}$-HSD protein was intensively localized in the large luteal cells and placental cytotrophoblast villus, glandular epithelial cells of the endometrium, syncytiotrophoblast of the placenta, the isthmus cells of the oviduct, and the basal part of the primary chorionic villi and chorionic stem villus of the placenta and large luteal cells of the CL in many mammalian species. Further studies are needed to determine the functional significance of the $20{\alpha}$-HSD molecule during ovulation, pregnancy, and parturition. This article will review how fundamental information of these enzymes can be exploited for a better understanding of the reproductive organs during ovulation and pregnancy.

• Journal of Wetlands Research
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• v.22 no.2
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• pp.73-80
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• 2020

Polygonatum stenophyllum Maxim. is a native perennial herb to Korea belonging to family Liliaceae. Although it was removed from the endangered species list, since it inhabits the riverside, it has been threatened with damage to its populations due to frequent disturbances. In order to reveal the basic ecological characteristics of P. stenophyllum, This study was identified the life cycle of the aboveground part and was measured the number of flowers and fruits, which are reproductive organs of P. stenophyllum in riverside in Paju - si during the two years. As a result, aboveground part of P. stenophyllum appeared and grew rapidly until the end of May to about 1m. In early May, two buds per one peduncle were formed up to six pairs per floor. The flowers bloomed in turn from the base of the stem and to the top floor and the blooming occurred when the number of leaves was at least three. Fruits were formed in mid-June and matured by the end of September. The numbers of flowers and fruits were revealed that flowers bloomed in turn from the bottom of the stem to the top floor, the most intensively bloomed in the center part of the stem, and did not bloom from the floor closest to the ground and above the highest 5th node. This basic ecological characteristics of the P. stenophllyum grown in riverside identified in this study will be useful as basic data for their conservation.

• Journal of Life Science
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• v.29 no.4
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• pp.447-454
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• 2019

Fat accumulation in adipocytes occurs through the process of adipogenesis in which preadipocytes differentiate into adipocytes. Obesity is a metabolic disorder caused by excessive accumulation of fat in the body, which increases the incidence of cardiovascular diseases, hypertension, type 2 diabetes, hyperlipidemia, and various cancers. Recently, inhibition of adipocyte differentiation was shown to be a potential antiobesity strategy. In this study, the inhibitory effect of dichloromethane fractions from Illicium verum Hooker fil. water extract on the differentiation of 3T3-L1 preadipocytes to adipocytes was investigated. Dichloromethane fractions from I. verum Hooker fil. significantly inhibited adipocyte differentiation when applied during the adipocyte differentiation process, as assessed by measuring fat accumulation using Oil-red O staining. In addition, dichloromethane fractions from I. verum Hooker fil. reduced important adipogenic transcription factors, such as CCAAT/enhancer binding protein ${\alpha}$ ($C/EBP{\alpha}$), $C/EBP{\beta}$, and peroxisome proliferator activated receptor ${\gamma}$ ($PPAR{\gamma}$). The expression of FAS and LPL, which are terminal differentiation markers of mature adipocytes, was also reduced in the 3T3-L1 adipocytes treated with dichloromethane fractions from I. verum Hooker fil. In addition, the treatment significantly inhibited mitotic clonal expansion, which is essential for adipocyte differentiation, by arresting the G1 phase of the cell cycle. Taken together, these results suggest that dichloromethane fractions from I. verum Hooker fil. may be a natural material with antiobesity effects.

• Korea journal of population studies
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• v.25 no.2
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• pp.163-192
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• 2002

Residential overcrowding, also called density, measured as more than one person per room, is a important variable as a principal indicator of inadequate housing. We investigated how immigrants in the US are assimilated to the host society through a lens of housing density. We estimated the probability of living in overcrowded housing of Korean, Chinese and Japanese immigrants armored with the 1980 and 1990 Public Use Microdata Sample(PUMS, A, 5%) in the US. Along with economic effects, we also consider life-cycle effects on the indicator. We applied "double cohort" method that enables duration of immigration effects to be separated from aging effects, which captures family size fluctuations due to life-cycle effects. The study found that cohort trends sharply changed during 10years. The 1970's immigrants are more likely to live in overcrowded housing than the pre-1970 immigrants. The pre-1970 immigrants are more likely to live in overcrowded housing than native-born persons. This may be explained by different assimilation processes driven by the disparities of individual human capitals or cultural differences among the ethnic groups. Especially, Korean experienced a sharp decline in overcrowding between 1980 and 1990. We also found that the major determinants that affect the level of housing density are years since migration, income, and gender. The present study concluded with some future studies related to the Korean immigrants abroad.ts abroad.

• The Korean Journal of Malacology
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• v.28 no.1
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• pp.13-19
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• 2012

To determine the status of Korean molluscs infection and relation with climate change of Korean peninsula, references were reviewed. Wild animals serve as intermediate, reservoirs and paratenic hosts to zoonotic parasites of human beings. Trematode are common parasites of molluscs and almost all trematodes infect mollusks as the first host in the life cycle, and most have a complex life cycle involving other hosts. The significance of auxiliary hosts to the ecology of the parasite has not been proper discussion. There is increasing concern as to the impact of climate change on the epidermiology of many parasitic diseases. A total of 21 species for snail transmitted parasites from Korean molluscs has been reviewed. Among them, 15 species was aquatic mollusks and 6 species for marine mollusks. Maximum infections belonged to Semisulcospira livertina had 11 kind of parasite pathogenic organisms, including Paragonimus westermani, Metagonimus yokogawai, Centrocestus armatus, Notocotylus magniovatus, Centrocestus formosanus, incerte cercaria, nipponensis cercaria, Yoshidae cercaria, cristata cercaria, innominatum cercaria and Metagonimus sp. And 11 in Parafossarulus manchouricus including Clonorchis sinensis, Asymphylodora japonica, Cyathocotyle orientalis, Exorchis oviformis, Notocotylus attenuatus, Echinochasmus japonicus, Loxogenes liberum, Cercariae of Loxogenes liberum Type I, Cercariae of Loxogenes liberum Type II, Furcocercus cercariae (Family Sanguincolidae) and Cercaria of Mucobucaris, 10 in Semisulcospira sp. including Paragonimus westermani, Metagonimus yokogawai, Centrocestus armatus, Echinochasmus redioduplicatus, Notocotylus magniovatus, Cercaria incerte, Cercaria nipponensis, Cercaria yoshidae, paludinarum cercaria and Metagonimus sp., 7 in Koreanomelania globus including Pseudexorchis major, Cercaria of parapleurolophocercous type, Metagonimus sp. (A & B), Cercaria nipponensis, Cercaria inserta and Cercaria yoshidae. Also, Tapes philippinarum have 3 pathogenic organisms including Cercariae tapidis, Cercariae furcocercus and Parvatrema sp. In particular, under climatic extremes such as floods and drought, aquatic molluscs may play a more prominent role in parasite transmission in the future.

• Journal of Life Science
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• v.15 no.1 s.68
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• pp.124-131
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• 2005

The regulation of gene expression plays an important rolet in cell cycle controls. In this study, a novel $mas3^+$ (mitosis associated protein) gene, a homolog of human SMARCADl, was isolated and characterized from a fission yeast Schizosaccharomyces pombe. The overall homology between the helicase proteins of the two species is $87\%$. This DEAD/H box-containing molecule has seven highly conserved sequence regions that allow us to place it in the SNF2 family of the helicase superfamily. Knock-out cell of $mas3^+$ gene was constructed using kanMX6 as a selection marker. Survival of mas3 null mutant exposed to UV or MMS was similar to those of wild type cells. $mas3^+$ expression was lowest at $G_2$ and gradually increased. Cytokinesis of mas3 null mutant was abnormal at $26^{\circ}C\;and\;35^{\circ}C$ and a large number of multi-septate cells were produced. These results indicate that the $mas3^+$ is involved in cytokinesis and cell shape control.

• Child Health Nursing Research
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• v.5 no.2
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• pp.185-197
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• 1999

In the family life cycle, the most important task the families with school children should perform is ‘child education’ and ‘socialization’ However, economic stress on poor families with school children presents multiple problems through the shortage of resources necessary for child education and socialization, inappropriateness of family appraisal, and the insufficiency of the control channels for the efficient management of these confined resources and appropriate appraisal. The objectives of this article are : First, to report research on the actual conditions of the poor families in one area of Cheju and on the relevant health welfare policy, and to examine the appropriateness of the direction and the substance of this policy in terms of the intervention in economic stress on the poor families under the categories of resource management and control of appraisal. Second, to analyze qualitative data extracted from the memoirs of single-parent families living in several areas of Korea under the conceptual framework constructed by literature review in order to get a better understanding of the stress which poor families with school children have experienced. And third, to confirm the factors that can be risk factor but, at the same time, strength to these poor families from presented data and to use them as the basic data from which an intervention model can be developed, based on resource management and control of appraisal. The findings of this article are : First, while the number of absolute poor families in one area of Cheju is increasing and, as a result, the danger of the possibility that multiple problems will occur is also growing, the supporting level of the current policy is no more than a direct resource offer and there is no evidence that resource management is being professionally carried out. When it comes to control of appraisal, due to absence of the professional human resources in this matter, policy performances such as technical education training can have a negative impact. Second, a conceptual framework introduced in this article, ; Economic Pressure → Helplessness → Poor self-esteem → Marital Conflict → Parent-Child financial conflict → Inappropriate socialization → Poor child social competence : is partly verified. And third, judging from the results of the qualitative data, it is confirmed that the healing families, having overcome poverty, show several positive characteristics including : hope, strength, and social support. These findings are identified with the factors of resiliency considered above. According to these results, this article suggests the following. The anti -poverty policy in the future should focus not only on a direct resource offer but also on resource management and the control of appraisal that can magnify its effects. In particular, close attention should be paid to school children since they are in the most crucial period for socialization. Moreover, an emotional labor is such an essential resource for intervention that skilled nurses should play pivotal roles.