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http://dx.doi.org/10.5352/JLS.2005.15.1.124

Studies on Intracellular Functions of the mas3 Gene in the Fission Yeast, Schizosaccharomyces pombe  

Hwang Mi Ra (Division of Biological Sciences, Wonkwang university)
Cha Jae Young (Division of Biological Sciences, Wonkwang university)
Shin Sang Min (Division of Biological Sciences, Wonkwang university)
Park Jong Kun (Division of Biological Sciences, Wonkwang university)
Publication Information
Journal of Life Science / v.15, no.1, 2005 , pp. 124-131 More about this Journal
Abstract
The regulation of gene expression plays an important rolet in cell cycle controls. In this study, a novel $mas3^+$ (mitosis associated protein) gene, a homolog of human SMARCADl, was isolated and characterized from a fission yeast Schizosaccharomyces pombe. The overall homology between the helicase proteins of the two species is $87\%$. This DEAD/H box-containing molecule has seven highly conserved sequence regions that allow us to place it in the SNF2 family of the helicase superfamily. Knock-out cell of $mas3^+$ gene was constructed using kanMX6 as a selection marker. Survival of mas3 null mutant exposed to UV or MMS was similar to those of wild type cells. $mas3^+$ expression was lowest at $G_2$ and gradually increased. Cytokinesis of mas3 null mutant was abnormal at $26^{\circ}C\;and\;35^{\circ}C$ and a large number of multi-septate cells were produced. These results indicate that the $mas3^+$ is involved in cytokinesis and cell shape control.
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