• Korean Journal of Social Welfare Studies
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• no.37
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• pp.199-240
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• 2008

This study set out to investigate the adaptation process and experiential structure of those children who went through sexual abuse by looking into their inner worlds in order to understand what kind of meaning sexual abuse had on them. For that, the investigator conducted in-depth interviews with 13 children aged 8~16 who lived at the shelter after experiencing sexual abuse. The methodology of Grounded Theory by Strauss and Corbin(1990) was used to analyze raw data. The analysis results indicate that the core theme of the adaptation process among the children living at the shelter after sexually abused by kin and kith was "hoping to appear the same as others." According to the results, the core phenomenon was "blaming the victims." The causal conditions include "broken families," "antihuman sexual abuse," "making sexual abuse a public issue," and "the trap of the family." The contextual conditions include "the chain named family," "family as the last fortress" and "structural enforcement of silence." The intervening strategies was "dual emotions toward the shelter." The action/interaction strategies include "aftermath of violence" and "trying to escape." The consequences were "preparation for the future" and "uncertain future." The identified stages include the confusion, keeping the secret, leaking the secret to others, intervention by others, social support and challenge and adjustment stage. The three identified types were "withdrawal and avoidance," "settling down in reality" and "overcoming and challenging." Based on the analysis results, discussions were made about the social welfare plans and intervention strategies in the conclusion.

• Journal of the Architectural Institute of Korea Planning & Design
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• v.36 no.2
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• pp.13-24
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• 2020

This study derives the characteristics of the private external space planning in multi-family housing through the analysis of the good case built after 2000 in Europe. First, the cases were categorized into block or block perimeter, linear and point type to examine the relationship between the type of building in urban context and the location of private external space. By block or block perimeter and linear type, private external spaces are planned in the inner courtyards or open space between buildings used as common space for residents, inducing communications between neighbors. And the direction of private external space depends on the arrangement of the building mass in urban context. In the classification as point type, there are many cases, where private external spaces are arranged in all directions, connected almost all interior spaces. Second, based on the above results, the planned characteristics of the private external space are derived by dividing it into three categories: intermediated space between inside and outside, intermediate space between private and public /individual and collective space and the identity of the intermediate space. (1) In most cases, direction, size of enclosed area and location of private extern space is designed to fit the surrounding context, so residents can perceive as much of the assets of the surrounding environment as possible, and it can be used as an extended area of living space. In another cases, it is divided into various sub-areas to experience the spatial transition from inside to outside or vice versa. 2) The private external space, which is placed in a courtyard or in a collective open space, is partially enclosed and blocked, allowing interaction with the neighbors without pressure. Along the street, they are designed to allow residents to experience the vitality of the city and to be formative element of the facade, which could confidently reveal the lifestyle and taste of residents. 3) By some of point types, which facade is three-demensional layer as a habitable external space, the private external space is very flexible for use. This intermediate space is composed of diverse spaces for various needs, or it has generous size with positional conditions connected with all interior spaces to be used multi-functional.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1996.04a
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• pp.182-182
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• 1996

Annexin I is a member of the annexin family of calcium dependent phospholipid binding proteins and has anti-inflammatory activity by inhibiting phospholipase A$_2$ (PLA$_2$). Recent X-ray crystallographic study of annexin I identified six Ca$\^$2+/ binding bites, which was different types (type II, III) from the well-known EF-hand motif (type I). In this work, the structure of annexin I was studied at atomic level by using $^1$H, $\^$15/N and $\^$l3/C NMR(nuclear magnetic resonance) spectroscopy, and the effect of Ca$\^$2+/ binding on the structure of annexin I was studied, and compared with that of Mg$\^$2+/ binding, When Ca$\^$2+/ was added to annexin I, NMR peak change was occured in high- and low-field regions of $^1$H-NMR spectra. NMR peak change by Ca$\^$2+/ binding was different from that by Mg$\^$2+/ binding. Because annexin I is a larger protein with 35 kDa molecular weight, site-specific (amide-$\^$15/N, carbonyl-$\^$l3/C) labeling technique was also used. We were able to detect methionine, tyrosine and phenylalanine peaks respectively in $\^$13/C-NMR spectra, and each residue was able to be assigned by the method of doubly labeling annexin I with [$\^$13/C] carbonyl-amino acid and [$\^$15/N] amide-amino acid. In $\^$l3/C-NMR spectra of [$\^$13/C] carbonyl-Met labeled annexin I, we observed that methionine residues spatially located near Ca$\^$2+/ binding Sites Were Significantly effected by Ca$\^$2+/ binding. From UV spectroscopic data on the effect of Ca$\^$2+/ binding, we knew that Ca$\^$2+/ binding sites of annexin I have cooperativity in Ca$\^$2+/ binding. The interaction of annexin I with PLA$_2$ also could be detected by using heteronuclear NMR spctroscopy. Consequently, we expect that the anti-inflammatory action mechanism of annexin I may be a specific protein-protein interaction. The residues involved in the interaction with PLA$_2$ can be identified as active site by assigning NMR peaks effected by PLA$_2$ binding.

• Advances in Traditional Medicine
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• v.6 no.4
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• pp.274-285
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• 2006

Allium sativum (Family Amaryllidaceae or Liliaceae) is used worldwide for various clinical uses like hypertension, cholesterol lowering effect, antiplatelets and fibrinolytic activity etc. Due to these common house hold uses of Allium sativum, as a herbal supplements, and failure of patients to inform their physician of the over-the-counter supplements they consume leads to drugnutrient interactions with components in herbal supplements. Today these types of interactions between a herbal supplement and clinically prescribed drugs are an increasing concern. In vitro studies indicated that garlic constituents modulated various CYP (cytochrome P450) enzymes. CYP 3A4 is abundantly present in human liver and small intestine and contributes to the metabolism of more than 50% of commonly used drugs including nifedipine, cyclosporine, erythromycin, midazolam, alprazolam, and triazolam. Extracts from fresh and aged garlic inhibited CYP 3A4 in human liver microsomes. The in vivo effects of garlic constituents are found to be species depended and the dosing regimen of garlic constituents appeared to influence the modulation of various CYP isoforms. Studies have indicated that the inhibition of various CYPs by organosulfur compounds from garlic was related to their structure also. Studies using in vitro, in vivo, animal and human models have indicated that various garlic constituents can be the substrates, inhibitors and or inducers of various CYP enzymes. The modulation of CYP enzyme activity and expression are dependent on the type and chemical structure of garlic constituents, dose regime, animal species and tissue, and source of garlic thus this review throws light on the possible herb drug interaction with the use of garlic.

• Journal of Microbiology and Biotechnology
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• v.26 no.12
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• pp.2019-2029
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• 2016

Zinc finger proteins are among the most extensively applied metalloproteins in the field of biotechnology owing to their unique structural and functional aspects as transcriptional and translational regulators. The classical zinc fingers are the largest family of zinc proteins and they provide critical roles in physiological systems from prokaryotes to eukaryotes. Two cysteine and two histidine residues ($Cys_2His_2$) coordinate to the zinc ion for the structural functions to generate a ${\beta}{\beta}{\alpha}$ fold, and this secondary structure supports specific interactions with their binding partners, including DNA, RNA, lipids, proteins, and small molecules. In this account, the structural similarity and differences of well-known $Cys_2His_2$-type zinc fingers such as zinc interaction factor 268 (ZIF268), transcription factor IIIA (TFIIIA), GAGA, and Ros will be explained. These proteins perform their specific roles in species from archaea to eukaryotes and they show significant structural similarity; however, their aligned amino acids present low sequence homology. These zinc finger proteins have different numbers of domains for their structural roles to maintain biological progress through transcriptional regulations from exogenous stresses. The superimposed structures of these finger domains provide interesting details when these fingers are applied to specific gene binding and editing. The structural information in this study will aid in the selection of unique types of zinc finger applications in vivo and in vitro approaches, because biophysical backgrounds including complex structures and binding affinities aid in the protein design area.

• Journal of Convergence for Information Technology
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• v.8 no.3
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• pp.33-38
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• 2018

Objective: To review the literature on development in the peer relationships of the adolescents within the context of their parents' divorce. Method: The cognitive and sociocultural literature was searched to analyze the social interactions of the adolescents of divorced parents. Results: The evidence supports the variations in adolescents' peer relations based on their family structure. Comparisons with the adolescents of the nuclear families and the step-families suggest certain types of interactions the adolescents of divorced parents experience. Conclusions: Divorce of the parents is associated with the difficulties their children at adolescent experience, such as the higher level of social isolation among their peers or their incompetence in social skills. For there is not much known about the integration into the world of peers of the adolescents from the divorced families, while their general psychological and cognitive development after the separation is often studied, future study will need to focus on their peer interactions at their school environment and the social treatment for the possible hardships in their peer relations.

• Molecules and Cells
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• v.44 no.9
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• pp.637-646
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• 2021

Free fatty acids are converted to acyl-CoA by long-chain acyl-CoA synthetases (ACSLs) before entering into metabolic pathways for lipid biosynthesis or degradation. ACSL family members have highly conserved amino acid sequences except for their N-terminal regions. Several reports have shown that ACSL1, among the ACSLs, is located in mitochondria and mainly leads fatty acids to the β-oxidation pathway in various cell types. In this study, we investigated how ACSL1 was localized in mitochondria and whether ACSL1 overexpression affected fatty acid oxidation (FAO) rates in C2C12 myotubes. We generated an ACSL1 mutant in which the N-terminal 100 amino acids were deleted and compared its localization and function with those of the ACSL1 wild type. We found that ACSL1 adjoined the outer membrane of mitochondria through interaction of its N-terminal region with carnitine palmitoyltransferase-1b (CPT1b) in C2C12 myotubes. In addition, overexpressed ACSL1, but not the ACSL1 mutant, increased FAO, and ameliorated palmitate-induced insulin resistance in C2C12 myotubes. These results suggested that targeting of ACSL1 to mitochondria is essential in increasing FAO in myotubes, which can reduce insulin resistance in obesity and related metabolic disorders.

• Korean Journal of Social Welfare
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• v.67 no.2
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• pp.33-60
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• 2015

The purpose of this study was to analyze the life experiences of fifteen marriage immigrant Asian women who went through running away from their home countries, marrying with Korean men, divorcing from their husbands, and coping with many difficulties after their divorce in Korean society. In order to conduct this study grounded theory methods have been employed. The central phenomenon digged out from this study was 'resistance to baffled reality' (i. e. dislocation). The causal conditions which brought about the central phenomenon were 'escaping for survival' and 'experiencing the gap between reality and expectation. 'The intervening conditions included 'getting to know the reality of their husbands,' 'losing hope,' and 'not being able to pull themselves together.' The contextual conditions consisted of 'being treated as maids,' 'becoming victims of family violence,' 'making up their minds to survive,' 'securing future life,' 'being marginalized,' and 'being aware of themselves as strangers.' The action/interaction strategies on the central phenomenon were 'building support systems,' 'building up will for new life,' and 'reconstructing social identity.'The final outcome was 'arranging places of new settlement.' The divorce was classified as four types: 'coping and growth,' 'emancipation and settling down,' 'being overwhelmed by livelihood,' and 'continuous wandering.' Based on these results, this study provided a few political and practice suggestions to prevent family violence and divorces among multi-cultural families, and also to bumper the impacts of divorce.

• Korean Journal of Social Welfare
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• v.62 no.3
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• pp.271-297
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• 2010

The purpose of this study was to develop the entity theory on the recovering process from gambling addiction. This study focused on "What is the process of getting out of the pit of gambling addiction?" based on grounded theory methodology suggested by Strauss and Corbin(1990). The subjects of this study were 7 male gambling addicts living in Jeonbuk area. As the result of this study, a total of 97 concepts were drawn, which were classified into 28 subcategories and 16 superordinate categories. The results of analyzing the categories by paradigm showed that in the process of overcoming gambling addiction, the casual condition was 'Being cut off money; 'Losing the will', and the contextual condition corresponding to this phenomenon was 'Shame', 'Repent', and 'Wanting to die'. The core phenomenon was 'Giving up the dream of hitting the jackpot'. The intervening condition to boost this core phenomenon was 'Being helped by friends', 'Being supported by the police', 'Being led by the family', and 'Showing the willpower'. The action/interaction strategies were identified as 'Cutting off the flow of funds', 'Overcoming the temptation of gambling', and 'Diverting attention to other things', ending up 'Being conscious of family', 'Growing up', and 'Being free'. The core concept in the process of overcoming the gambling addiction was revealed as 'Being free by giving up the dream of hitting the jackpot'. The process of getting over the gambling addiction was 'The dream of hitting the jackpot being shattered', 'Giving up the dream of hitting the jackpot', 'Getting out of it', and 'Being free'. The study participants were classified into four types; 'Challenging type', 'Enjoying type', 'Showing off type', and 'Obsessive type'. This study was expected to have significance in establishing the first theory in Korea on the recovering process of gambling addict.

• Journal of Life Science
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• v.30 no.5
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• pp.483-490
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• 2020

The ubiquitin system uses ligases and deubiquitinases (DUBs) to regulate ubiquitin position on protein substrates and is involved in many biological processes which determine stability, activity, and interaction of the target substrate. DUBs are classified in six groups according to catalytic domain, namely ubiquitin-specific proteases (USPs); ubiquitin C-terminal hydrolases (UCHs); ovarian tumor proteases (OTUs); Machado Joseph Disease proteases (MJDs); motif interacting with Ub (MIU)-containing novel DUB family (MINDY); and Jab1/MPN/MOV34 metalloenzymes (JAMMs). Otubain 1 (OTUB1) is a DUB in the OTU family which possesses both canonical and non-canonical activity and can regulate multiple cellular signaling pathways. In this review, we describe the function of OTUB1 through regulation of its canonical and non-canonical activities in multiple specifically cancer-associated pathways. The canonical activity of OTUB1 inhibits protein ubiquitination by cleaving Lys48 linkages while its non-canonical activity prevents ubiquitin transfer onto target proteins through binding to E2-conjugating enzymes, resulting in the induction of protein deubiquitination. OTUB1 can therefore canonically and non-canonically promote tumor cell proliferation, invasion, and drug resistance through regulating FOXM1, ERα, KRAS, p53, and mTORC1. Moreover, clinical research has demonstrated that OTUB1 overexpresses with high metastasis in many tumor types including breast, ovarian, esophageal squamous, and glioma. Therefore, OTUB1 has been suggested as a diagnosis marker and potential therapeutic target for oncotherapy.