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Development of strain-specific polymerase chain reaction primers to detect Fusobacterium hwasookii strains

  • Lim, Yun Kyong;Kook, Joong-Ki
    • International Journal of Oral Biology
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    • v.46 no.4
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    • pp.155-159
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    • 2021
  • This study aimed to develop strain-specific polymerase chain reaction (PCR) primers to detect Fusobacterium hwasookii KCOM 1249T, F. hwasookii KCOM 1253, F. hwasookii KCOM 1256, F. hwasookii KCOM 1258, and F. hwasookii KCOM 1268 on the basis of nucleotide sequences of a gene specific to each strain. The unique genes for each F. hwasookii strain were determined on the basis of their genome sequences using Roary. The strain-specific PCR primers based on each strain-specific gene were designed using PrimerSelect. The specificity of each PCR primer was determined using the genomic DNA of the 5 F. hwasookii strains and 25 strains of oral bacterial species. The detection limit and sensitivity of each strain-specific PCR primer pair were determined using the genomic DNA of each target strain. The results showed that the strain-specific PCR primers correspond to F. hwasookii KCOM 1249T, F. hwasookii KCOM 1253, F. hwasookii KCOM 1258, F. hwasookii KCOM 1256/F. nucleatum subsp. polymorphum KCOM 1260, or F. hwasookii KCOM 1268/Fusobacterium sp. oral taxon 203 were developed. The detection limits of these strain-specific PCR primers ranged from 0.2 to 2 ng of genomic DNA for each target strain. The results suggest that these strain-specific PCR primers are valuable in quality control for detecting specific F. hwasookii strains.

DECOMPOSITION FORMULAS AND INTEGRAL REPRESENTATIONS FOR THE KAMPÉ DE FÉRIET FUNCTION F0:3;32:0;0 [x, y]

  • Choi, Junesang;Turaev, Mamasali
    • Journal of the Chungcheong Mathematical Society
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    • v.23 no.4
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    • pp.679-689
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    • 2010
  • By developing and using certain operators like those initiated by Burchnall-Chaundy, the authors aim at investigating several decomposition formulas associated with the $Kamp{\acute{e}}$ de $F{\acute{e}}riet$ function $F_{2:0;0}^{0:3;3}$ [x, y]. For this purpose, many operator identities involving inverse pairs of symbolic operators are constructed. By employing their decomposition formulas, they also present a new group of integral representations of Eulerian type for the $Kamp{\acute{e}}$ de $F{\acute{e}}riet$ function $F_{2:0;0}^{0:3;3}$ [x, y], some of which include several hypergeometric functions such as $_2F_1$, $_3F_2$, an Appell function $F_3$, and the $Kamp{\acute{e}}$ de $F{\acute{e}}riet$ functions $F_{2:0;0}^{0:3;3}$ and $F_{1:0;1}^{0:2;3}$.

EXTREMELY MEASURABLE SUBALGEBRAS

  • Ayyaswamy, S.K.
    • Bulletin of the Korean Mathematical Society
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    • v.22 no.1
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    • pp.7-10
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    • 1985
  • For each a.mem.S and f.mem.m(S), denote by $l_{a}$ f(s)=f(as) for all s.mem.S. If A is a norm closed left translation invariant subalgebra of m(S) (i.e. $l_{a}$ f.mem.A whenever f.mem.A and a.mem.S) containing 1, the constant ont function on S and .phi..mem. $A^{*}$, the dual of A, then .phi. is a mean on A if .phi.(f).geq.0 for f.geq.0 and .phi.(1) = 1, .phi. is multiplicative if .phi. (fg)=.phi.(f).phi.(g) for all f, g.mem.A; .phi. is left invariant if .phi.(1sf)=.phi.(f) for all s.mem.S and f.mem.A. It is well known that the set of multiplicative means on m(S) is precisely .betha.S, the Stone-Cech compactification of S[7]. A subalgebra of m(S) is (extremely) left amenable, denoted by (ELA)LA if it is nom closed, left translation invariant containing contants and has a multiplicative left invariant mean (LIM). A semigroup S is (ELA) LA, if m(S) is (ELA)LA. A subset E.contnd.S is left thick (T. Mitchell, [4]) if for any finite subser F.contnd.S, there exists s.mem.S such that $F_{s}$ .contnd.E or equivalently, the family { $s^{-1}$ E : s.mem.S} has finite intersection property.y.

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Sensory quality, antioxidant, and inhibitory activities of XO and AO of Smilax china leaf tea fermented by Aspergillus oryzae (Aspergillus oryzae 발효 청미래덩굴잎 분말차의 관능적 품질 및 항산화능과 xanthine oxidase 및 aldehyde oxidase 저해활성)

  • Lee, Sang-Il;Lee, Ye-Kyung;Kim, Soon-Dong;Yang, Seung Hwan;Suh, Joo-Won
    • Food Science and Preservation
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    • v.21 no.1
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    • pp.129-139
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    • 2014
  • This study was conducted in order to investigate the optimal fermentation periods of the Smilax china L. leaves as a fermented tea via Aspergillus oryzae for 0 (non-fermented), and 10, 20, and 30 days (NF, F10, F20, F30). It was also observed for its quality characteristics. In the color and spectrum (400~700nm) of 1% tea water extract, NF was light yellow, whereas fermented tea (F10~F30) was light red color, and the F10 among F10~F30 has the clearest color and spectrum. Furthermore, acceptabilities of aroma and brightness were insignificantly different between NF and F10~30, while the mouth feel and overall acceptabilities were insignificantly distinct among all of the fermented teas. Therefore, these results suggest that the appropriate fermentation period for tea fermentation is 10 days. On the other hand, the total polyphenol and flavonoid content in the NF was the highest among all of the fermented teas. In the antioxidant parameters, EDA (electron donating ability), FRAP (ferric reducing antioxidant power), and LPOIA (lipid peroxidation inhibitory activity) in the NF were the highest among all fermented teas. Meanwhile, the XOI (xanthine oxidase inhibitory activity) was low, as well as insignificantly different from NF and F10~F30, whereas the AOI (aldehyde oxidase inhibitory activity) was markedly higher (38.09~41.70%) by the hot water tea extract (with or without fermentation), particularly the AOI that has increased via fermentation. In conclusion, the overall antioxidant activity tended to be reduced by fermentation; however, the EDA, FRAP and LPOIA in the fermented tea for 10 days was higher than the activities during 20~30 days of fermentation. There was a similar result in the color and acceptability of fermented tea for 10 days, which was remarkably better than those of 20-30 days. Therefore, fermented tea from the leaves of Smilax china L. could be expected to be used as a functional tea without the loss of inhibitory activity of both the XO and AO via fermentation.

Electrical and optical properties of Al and F doped ZnO transparent conducting film by sol-gel method (Sol-gel법에 의한 Al과 F가 첨가된 ZnO 투명전도막의 전기 및 광학적 특성)

  • Lee, Seung-Yup;Lee, Min-Jae;Park, Byung-Ok
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.16 no.2
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    • pp.59-65
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    • 2006
  • Al-doped and F-doped ZnO (ZnO : Al & ZnO : F) thin films were coated onto glass substrate by sol-gel method. These films showed c-axis orientation in common, but different I(002)/[I(002) + I(101)] and FWHM (full width at half-maximum). In particular, the grain size of the ZnO : Al films decreased with the increase in the Al-doping concentration, while for the ZnO : F films the grain siae increased up to F 3 at% and then decreased. For the electrical properties, Hall effect measurement was used. The resistivity of the ZnO : Al films and the ZnO : F films were, respectively, $2.9{\times}10^{-2}{\Omega}cm$ at Al 1 at% and $3.3{\times}10^{-1}{\Omega}cm$ at F 3 at%. Moreover compared with ZnO:Al films, ZnO:F films have lower carrier concentration (ZnO : Al $4.8{\times}10^{18}cm^{-3}$, ZnO : F $3.9{\times}10^{16}cm^{-3}$) and higher mobility (ZnO : Al $45cm^2/Vs$, ZnO : F $495cm^2/Vs$). For average optical transmittances, ZnO : Al thin films have $86{\sim}90%$ and ZnO : F films have $77{\sim}85%$ comparatively low.

Rheological Characteristics and Molecular Weight of Ammonium-Sulfate Fractions of Tara Gum (염석법에 의한 타라검 분획들의 분자량 및 리올로지 특성)

  • Kim, Kyeong-Yee
    • Korean Journal of Food Science and Technology
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    • v.47 no.3
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    • pp.293-298
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    • 2015
  • This study aimed at characterizing the rheological properties and molecular weight of tara gum fractionated with ammonium sulfate. Tara gum was separated into six fractions (F1-F6) at different concentrations of ammonium sulfate, ranging from 12.21 to 28.67% (w/w). The yield of the tara gum fractions ranged between 4.98 and 17.47%, and their intrinsic viscosity ranged from 9.38 to 12.44 dL/g. The highest values of Huggins coefficient (k') and viscosity-molecular mass were observed in fraction F3. The shear viscosity of the tara gum fractions was measured by a cone-plate viscometer, clearly showing shear thinning behavior. Size-exclusion chromatography results showed that the molecular weight ranged between 635.42 and 776.71 kg/mol, and the F3 fraction exhibited higher values of molecular weight.

A Ka-band Harmonic Miter Design Using Multiplier Theory (체배기 이론을 이용한 Ka-대역 고조파 믹서 설계)

  • Go Min-Ho;Kang Suk-Youb;Park Hyo-Dal
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.30 no.11A
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    • pp.1104-1109
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    • 2005
  • In this paper, a Ka-band harmonic mixer is designed and fabricated on the base of the multiplier theory that there is a bias point to maximize the third harmonic order($3f_{LO}$) with respect to a fundamental LO frequency($f_{LO}$), which can make the high-order mixing element($f_{RF}{\pm}3f_{LO}$) to be greater than other mixing elements, Pumping a RF frequency($f_{RF}$) and LO frequency($f_{LO}$). The harmonic mixer by the proposed design method is fabricated by using a commercial GaAs MESFET device with a plastic package and overcome these disadvantages that a conventional mixer in Ka-band suffer from a high cost, inefficient productivity and circuit complexity. The harmonic mixer have a -10 dB conversion loss at the IF Sequency($3f_{LO}-f_{RF}$=1.0GHz) by selecting a gate bias voltage for the maximum third-order LO harmonic element($3f_{LO}$=34.5 GHz) as pumping LO frequency($f_{LO}$=11.5 GHz) With respect to RF Sequency ($f_{RF}$=33.5GHz)

Contested Technologies, Resetting the Boundary, and the "signifiant-politics": Semiotical Governance of New Technology in the Case of fMRA (경합하는 기술, 경계의 재설정, 그리고 기표-정치(signifiant-politics): 기능성자기공명혈관조영술(fMRA)의 사례로 살펴본 신기술의 명명 작업)

  • Lee, June-Seok
    • Journal of Science and Technology Studies
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    • v.14 no.2
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    • pp.199-222
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    • 2014
  • Functional Magnetic Resonance Angiography (fMRA) was a technoscientific innovation that allows scientists to directly view the changes made in the blood vessels of a brain. fMRA was first developed at Neuroscience Research Institute (NRI) in Korea. fMRA mainly utilizes 7 Tesla MRI technology, and NRI is equipped with the instrument. First article on fMRA was published in 2008, and two more papers in 2010 and 2012 consecutively had been published on the newly developed technique. However, fMRA is a competitive technology with existing fMRI. Both techniques capture microvascular changes in a brain, and by doing it, both techniques visualize the cognitive and affective changes. fMRI technology was introduced by Seiji Ogawa in the early 1990's and has been widely used since then. In contrast, fMRA was a newer technology and rather unknown. Developers of fMRA in NRI used series of signifiant-politics in order to make it better known to scientific community as well as public. By resetting the boundaries of existing concept of fMRI, they tried to lower the threshold of a new concept/technique. This case study shows how technoscientists use semiotic strategies governing new technology.

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Morphological, Cytological and Molecular Evidence for Intersubgeneric F1 Hybrid between Glycine max x G. tomentella (콩 Glycine max와 G. tomentella의 종간교잡으로부터 얻은 Fl식물체 검증을 위한 형태적 · 세포학적 · 분자유전학적 연구)

  • Choi, In-Soo;Kim, Yong-Chul
    • Journal of Life Science
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    • v.18 no.4
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    • pp.454-460
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    • 2008
  • This study was carried out to demonstrate morphological, cytological and molecular evidence for intersubgeneric $F_1$ hybrid between Glycine tomentella and G. max cv. ‘Baemkong’. Morphological features of $F_1$ plant for pistil and stamen, flower color and growth habit showed intermediate type between G. tomentella and G. max cv. ‘Baemkong’. Chromosome number of $F_1$ plant was 2n=39, which explained the evidence of $F_1$ hybrid between G. tomentella (2n=38) and G. max cv. ‘Baemkong’ (2n=40). Polyacrylamide isoelectric focusing pattern for esterase and peroxidase also showed that the $F_1$ plant was true $F_1$ hybrid between G. tomentella and G. max cv. ‘Baemkong’. From RAPD analysis, we identified that 62 primers showing bands in $F_1$ hybrid had both bands from G. tomentella and G. max cv. ‘Baemkong’, which suggested that this was true $F_1$ hybrid. Based on our results from morphological, cytological and molecular analyses, we suggest that the $F_1$ plant was true intersubgeneric hybrid between G. tomentella and G. max cv. ‘Baemkong’. Our results still remain us further study to recover fertility of $F_1$ hybrids. The occurrence of maternal and/or paternal inheritance in $F_1$ hybrid from intersubgeneric cross between G. tomentella and G. max cv. ‘Baemkong’ need to be explained.

Genetic Studies in Some Agronomic Characters in Rapeseed (Brassica napus L.) 1. Genetic analysis for maturity, plant height and grain weight per plant. (유채의 실용형질에 대한 유전연구 -제 1 보 유채의 초장, 성숙기 및 종실중의 유전분석-)

  • Lee, J.I.;Kwon, B.S.;Chae, Y.A.
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.29 no.1
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    • pp.84-88
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    • 1984
  • To obtain basic information on the breeding of early maturing, short plant height rapeseed varieties, the following 7 varieties, Isuzu, Miyuki, Norin 25, Rang, Yongdang, Cresus and Tower were used in diallel crosses in 1979. Maturing date, plant height and grain weight per plant for the parents, $F_1$'s and $F_2$'s of the 7 x 7 partial diallel crosses were measured in 1981 for analysis of their genetic behavior. The results obtained are summarized as follows: 1. The days to maturing of $F_1$'s showed complete dominance for early maturing, and both additive and dominance genetic variances were significant. Number of effective factors in $F_1$'s was 3, but in $F_2$'s was 1. The degree of dominance in $F_1$'s was partial, while in $F_2$'s was complete. Both broad and narrow sense herita-bilities in $F_1$'s was high, while in $F_2$'s was low. 2. Yield per plant in $F_2$'s was controlled by additive component of genetic variance only, but $F_1$'s was different. The degree of dominance in $F_1$'s was complete, while in $F_2$'s was partial. The direction of dominance showed almost complete dominance over high yield and three effective factors was estimated. Yield per plant was controlled by recessive genes. 3. The plant height was controlled by both dominance and additive variance. Dominance was directed toward tall plant height. Number of effective factors was 2, and broad and narrow sense heritability were high in the plant height.

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