• 제목/요약/키워드: f/2 media

검색결과 442건 처리시간 0.032초

소하은어의 병소로부터 Flavobcterium succinicans의 분리 및 동정 (Isolation and identification of Flavobcterium succinicans from anadromous ayu Plecoglossus altivelis)

  • 이창훈;김필연;임봉수;오덕철;강봉조
    • 한국어병학회지
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    • 제22권3호
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    • pp.401-406
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    • 2009
  • 2008년 5월 제주지역 하천에 소하하는 은어 중 일부개체가 주둥이 부식 또는 체표궤양증상을 보이면서 폐사하였다. 어류 질병원인균 중 부식증 및 체표궤양과 연관성이 높은 Flavobac-terium 속 세균의 분리를 위해 Shu-Shott medium 및 R2A medium에 주둥이 부식 및 체표궤양 부위를 도말하여 배양 하였고, Flavobacterium 속 세균의 전형적인 집락인 pale yellow colony를 분리하였다. 16S rDNA 염기서열 분석결과 Flavobacterium succinicans DSM 4002(Accession NO. AM230492)와 98.27%의 상동성을 보여 F. succinicans JMFL55로 동정하였다.

나노 잉크젯 DTP(Digital Textile Printing)의 컬러 재현성 및 내구성에 관한 연구 - 2012-2013 F/W COLOR TREND를 중심으로 - (The Study of the color reproducibility and the color fastness of Nano Inkjet DTP(Digital Textile Printing) - Focusing on 2012-2013 F/W COLOR TREND -)

  • 김소진;최경미
    • 패션비즈니스
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    • 제16권2호
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    • pp.138-150
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    • 2012
  • Nano Inkjet DTP technology, a new technology introduced recently, can be applied to various types of fabric, and pre-treatment process can be omitted, which makes the whole printing process compact. Some important factors for DTP are color difference between the color selected by the designer on PC and the color on the final product and durability of the final fabrics. In this study, the twenty-three trend colors of 2012-2013 F/W suggested by PeclersParis have been picked to be printed on cotton, silk and polyester fabrics, then K/S and ${\Delta}E$ value and color fastness were measured. The results show that dyeability of fabric is varied for each color group, and that also tone of color affect to dyeability when measured for colors in the same group. In general, for all fabric, light fastness, washing fastness and color fastness to sublimation are outstanding. However, because of poor rubbing fastness, additional treatment to fix colorant on fabric is required.

몰입형 입체영상 부호화를 위한 VVC 인루프 필터 성능 분석 (Performance Analysis of VVC In-Loop Filters for Immersive Video Coding)

  • 최용호;방건;이진호;이진영
    • 한국방송∙미디어공학회:학술대회논문집
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    • 한국방송∙미디어공학회 2022년도 추계학술대회
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    • pp.151-153
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    • 2022
  • 최근 Moving Picture Experts Group(MPEG)에서는 2차원 비디오 압축 표준인 Versatile Video Coding(VVC)에 이어서 다양한 영상 포맷들에 대한 압축 방식을 표준화하고 있다. 특히, 가상현실, 증강현실, 혼합현실 등의 지원을 위한 Six Degrees of Freedom(6DoF) 입체영상 콘텐츠들이 최근 다양한 분야들에서 활용되고 있는데, 6DoF 입체영상은 일반적으로 복수 시점의 고해상도 칼라영상과 깊이영상으로 구성된다. 이러한 고해상도의 6DoF 몰입형 입체영상을 제한된 네트워크 환경에서 완벽한 서비스를 목표로 MPEG에서는 몰입형 입체영상 압축 기술인 MPEG Immersive Video(MIV) 표준화를 활발하게 진행 중에 있다. MIV에서는 기본 뷰(Basic View)로 이루어진 영상과 추가 뷰(Addtional View)에서 중복성 높은 픽셀들이 제거된 아틀라스 패치로 이루어진 영상을 각각 VVC로 압축한다. 하지만 아틀라스 패치로 이루어진 영상의 경우에는 일반적인 2차원 칼라영상과 다른 특성을 가지기 때문에, VVC 인루프 필터 기술이 비효율적일 수 있다. 따라서, 본 논문에서는 MIV 표준에서의 VVC 인루프 필터들의 성능을 분석한다.

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한국 중년여성들의 유방자가검진(BSE)에 관한 지식, 태도 및 실천정도 (Knowledge, Attitude, and Practices of Breast Self-Examination of Middle-aged Women in Korea)

  • 박정숙;오윤정;최영희;박은아;정추자;장희정
    • 지역사회간호학회지
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    • 제13권4호
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    • pp.719-731
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    • 2002
  • Purpose: The purpose of this study was to identify knowledge, attitude, and practice of middle-aged women breast self-examination, and to develop a breast self-examination promotion program for them. Method: The subjects of the study were 365 middle-aged women in Seoul, Busan, Daegu, Daejeon, Gyeongbok, Gyeongnam, Jeonbuk, Gangwon, and Kyonggi. The instruments used in this study included knowledge (l6items), attitude (22items) by Choi (1996) and practice (1item). The data were collected from September 15 to October 31, 2001 by using self-administered questionnaire. The data were analyzed by using descriptive statistics, Pearson correlation coefficient, $x^2$ test, t-test, ANOVA and Scheffe test with SPSS program. Results: 1) 78.4% of the subjects were educated for breast self-examination. The most common source of knowledge for the breast self-examination was mess media. 2) The average score of knowledge on breast self-examination was 8.52 points. The average score of attitude toward breast self-examination was 3.08 points. 58.3% of the subjects practiced breast self-examination. But only 9.4% of the subjects practiced breast self-examination with a monthly basis. 3) Knowledge on breast self-examination was correlated with demographic variables including residence (F=2.923, p=0.004), education (F=5.145 p=0.000), number of children (F=5.125 p=0.002), family income (F=6.128 p=0.002), and menopause (t=-2.330, p= 0.020). Attitude toward breast self-examination was correlated with demographic variables including residence (F=3.611, p=0.000), age (F=2.731 p= 0.029), education (F=4.480 p=0.004), and family income (F=5.963 p=0.003). Practice of breast self-examination was correlated with demographic variables including residence ($x^2$=51.342, p=0.000), education ($x^2$=19.896, p=0.000), and menopause ($x^2$=9.841, p=0.000). 4) In terms of correlations among the scores of breast self-examination related knowledge, attitude, and practice, there was a correlation between knowledge and attitude. But there was no correlations between knowledge and practice, and between attitude and practice of breast self-examination. Conclusion: This study suggests that programs about the practice of breast self examination need to be developed in the future.

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몇 가지 여재를 이용한 부영양수 내의 조류 및 인 제거효과 (The Removal of Algae and Phosphorus in Eutrophic Waters Using Various Filter Media)

  • 박채홍;박명환;최동호;이준헌;이명훈;황순진
    • 생태와환경
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    • 제45권1호
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    • pp.102-109
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    • 2012
  • 본 연구는 자연수와 인공조류 배양수(BG-11 medium) 등 두 가지 부영양 실험수를 이용하여 네 가지의 여재(스펀지, 화산석, 활성탄, 수산화마그네슘)의 단독 및 혼합여재 적용에 따른 조류 및 인 제거효과를 조사하였다. 혼합여재는 컬럼 여과장치에 각각의 여재를 충진후, 스펀지, 화산석, 활성탄, 수산화마그네슘의 순서로 연결하여 단독으로 여과할 때와 서로 비교하였다. 또한 여재로 사용한 수산화마그네슘의 첨가량 및 입자크기를 조절하여 인 제거효과를 비교하였다. 수산화마그네슘은 두 가지 입경(2 mm 이상 및 이하) 모두에서 높은 인 제거효과를 보였으며, 반응시간 및 첨가량 증가에 따라 인 제거효과가 증가하였다. 단독여재의 적용 결과, 4가지 여재 중 활성탄은 조류 및 인 제거에 매우 효과적인 것으로 나타났다. 또한 활성탄을 이용한 단독여재와 비교하여 혼합여재를 통해 가장 높은 인 제거효율을 확인하였다. 이러한 결과를 토대로 혼합여재의 경우에는 제거효율이 높았던 활성탄과 수산화마그네슘 등의 조합 적용이 상승작용을 나타낸 것으로 사료된다.

Establishment of a linear regression equation for quantification of beta-hemolytic Escherichia coli in different media and survival of hemolytic Escherichia coli after blending with three different media

  • Kim, Jae Cheol;Pluske, John R.;Yoo, Jaehong;Heo, Jung Min
    • 농업과학연구
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    • 제41권2호
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    • pp.135-139
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    • 2014
  • Pathogenic E. coli associated post-weaning diarrhea (PWD) and edema disease are common diseases in commercially-housed weanling pigs. An enterotoxigenic E. coli (ETEC) oral challenge model has been used to mimic the physiological responses observed in commercial conditions. However, an oral challenge procedure has two major limitations: (1) the ETEC cell density is unknown at the point of oral inoculation, and (2) blending ETEC with traditional TSB (trypticase soy broth) is not palatable and hence decreases acceptability by piglets. Therefore, the purposes of this study were to (1) establish a regression equation that can be used for estimation of ETEC concentration in dilution media using the spectrophotometric measurement of cell density; and (2) examine survival of ETEC after blending either with TSB, sweetener or dextrose. A strain of ETEC (serogroup beta-hemolytic E. coli O149; K91; F4; toxins LT, STa, STb) was grown in TSB for 3.5 hours, centrifuged, the supernatant was discarded, and the ETEC pellet was then blended either with TSB (100 mL), sweetener (60 mL TSB + 40 mL fruit flavored concentrate), or dextrose (50 mL TSB + 50 mL dextrose; 0.5g/mL dextrose). Cell density was measured using the colorimetric method and also plated on a 5% sheep blood agar for counting of ETEC colony forming units at 0, 5, 35, 65 and 125 min after blending. The optical density at 600 nm explained 83% of ETEC colony forming units, indicating that the established linear equation (y= 6E+08x - 4E+07, P<0.004) can be used for robust quantification of ETEC cell density in TSB, sweetener and dextrose media. When ETEC was blended with sweetener and dextrose, survival of ETEC was decreased by 45% and 72% within 5 min post-blending. Therefore, further research is required to find out the suitable medium that has potential to improve palatability without compromising survival of ETEC.

Rapid Establishment of CHO Cell Lines Producing the Anti-Hepatocyte Growth Factor Antibody SFN68

  • Song, Seong-Won;Lee, Song-Jae;Kim, Chang-Young;Han, Byungryeul;Oh, Jong-Won
    • Journal of Microbiology and Biotechnology
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    • 제23권8호
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    • pp.1176-1184
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    • 2013
  • Anti-hepatocyte growth factor (anti-HGF) monoclonal antibodies (mAbs) are potential therapeutics against various cancers. Screening for high-producer clones is a time-consuming and complex process and is a major hurdle in the development of therapeutic mAbs. Here, we describe an efficient approach that allows the selection of high-producer Chinese hamster ovary (CHO) cell lines producing the novel anti-HGF mAb SFN68, which was generated previously by immunizing HGF bound to its receptor c-Met. We selected an SFN68-producing parental cell line via transfection of the dihydrofolate reductase-deficient CHO cell line DG44, which was preadapted to serum-free suspension culture, with an SFN68-expression vector. Subsequent gene amplification via multiple passages of the parental cell line in a methotrexate-containing medium over 4 weeks, followed by clonal isolation, enabled us to isolate two cell lines, 2F7 and 2H4, with 3-fold higher specific productivity. We also screened 72 different media formulated with diverse feed and basal media to develop a suboptimized medium. In the established suboptimized medium, the highest anti-HGF mAb yields of the 2F7 and 2H4 clones were 842 and 861 mg/l, respectively, which were about 10.5-fold higher than that of the parental cell line in a non-optimized basal medium. The selected CHO cell lines secreting high titers of SFN68 would be useful for the production of sufficient amounts of antibodies for efficacy evaluation in preclinical and early clinical studies.

A MULTISCALE MORTAR MIXED FINITE ELEMENT METHOD FOR SLIGHTLY COMPRESSIBLE FLOWS IN POROUS MEDIA

  • Kim, Mi-Young;Park, Eun-Jae;Thomas, Sunil G.;Wheeler, Mary F.
    • 대한수학회지
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    • 제44권5호
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    • pp.1103-1119
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    • 2007
  • We consider multiscale mortar mixed finite element discretizations for slightly compressible Darcy flows in porous media. This paper is an extension of the formulation introduced by Arbogast et al. for the incompressible problem [2]. In this method, flux continuity is imposed via a mortar finite element space on a coarse grid scale, while the equations in the coarse elements (or subdomains) are discretized on a fine grid scale. Optimal fine scale convergence is obtained by an appropriate choice of mortar grid and polynomial degree of approximation. Parallel numerical simulations on some multiscale benchmark problems are given to show the efficiency and effectiveness of the method.

간접(間接) 포기식 유동상(流動床)의 운전조건(運轉條件) 변화에 따른 처리(處理) 특성(特性)에 관한 연구 -오니전환율 및 산소이용속도를 중심으로- (A Study on the Wastewater Treatment Characteristics according to Operating Condition of the Indirectly Aerated Fluidized Bed)

  • 이정수
    • 상하수도학회지
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    • 제11권3호
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    • pp.79-86
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    • 1997
  • The characteristics of wastewater treatment according to operating condition of indirectly aerated fluidized bed filled with sand media were studied experimentally, and the obtained results were as follows; 1. During be operated under the BOD loading rate, $4.5kg-BOD/m^3{\cdot}day$, effluent SS according to the increase of recirulation velocities was gradually decreased. 2. According to the increase of media concentration, sludge convertion rate was gradually increased. 3. Under the same BOD loading rate, according to the increase of recirulation velocities, F/M ratio was gradually increased, however, sludge convertion rate was greatly decreased. On the other hand, inffluent BOD concentration has not an important effect. 4. Under the constant recirulation velocities, according to the increase of BOD loading rate, sludge convertion rate was gradually increased. 5. Under the same BOD loading rate, according to the increase of recirulation velocities, oxygen consumpion rate of microorgnism was gradually increased.

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V3C 비트스트림 기반 ISOBMFF 캡슐화 실험 (ISOBMFF encapsulation experiment based on the V3C bitstream)

  • 남귀중;김준식;김규헌
    • 한국방송∙미디어공학회:학술대회논문집
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    • 한국방송∙미디어공학회 2021년도 하계학술대회
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    • pp.154-156
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    • 2021
  • 최근 3차원 영상이 다양한 분야에서 활용되고 있으며, 이에 따라 3차원 영상에 대한 압축과 전송 방안에 대한 연구가 활발히 진행되고 있다. 국제 표준화 기구인 ISO/IEC 산하 Moving Picture Expert Group(MPEG)에서는 기존의 2차원 비디오 코덱을 이용하여 고밀도 포인트 클라우드 압축하는 방안인 V-PCC와 3DoF+ 영상을 압축하기 위한 방안인 MPEG Immersive Video(MIV)를 표준화 중에 있다. V-PCC와 MIV는 압축 방법의 유사성으로 인해 동일한 Volumetric Visual Video-based Coding(V3C) 형식으로 저장된다. 압축된 V3C 데이터를 효과적으로 저장하여 이용하기 위해서는 ISO based Media File Format(ISOBMFF) 캡슐화 과정이 필수적이다. 본 논문에서는 MPEG의 Carriage of V3C data 표준에 따라 V3C 데이터를 ISOBMFF로 캡슐화 실험을 진행하였으며, 실험에 대한 검증을 위하여 생성된 ISOBMFF 데이터를 V3C 데이터로 복원한 뒤, 디코딩 하여 확인하였다.

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