• The Plant Pathology Journal
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• v.25 no.2
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• pp.113-120
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• 2009

Microscopic study of chili pepper (Capsicum annuum L.) infected with Phytophthora capsici, causing Phytophthora blight of chili pepper, was conducted to compare histological and cytological characteristics in the root and stem of susceptible (C. annuum cv. Bugang) and resistant (C. annuum cv. CM334) pepper cultivars. The susceptible pepper roots and stems were extensively penetrated and invaded by the pathogen initially into epidermal cells and later cortical and vascular cells. Host cell walls adjacent to and invaded by the infecting hyphae were partially dissolved and structurally loosened with fine fibrillar materials probably by cell wall-degrading enzymes of the pathogen. In the resistant pepper, the pathogen remained on root epidermal surface at one day after inoculation, embedded and captured in root exudation materials composed of proteins and polysaccharides. Also the pathogen appeared to be blocked in its progression at the early infection stages by thickened middle lamellae. At 3 days after inoculation, the oomycete hyphae were still confined to epidermal cells of the root and at most outer peripheral cortical cells of the stem, resulting from their invasion blocked by wound periderms formed underneath the infection sites and/or cell wall appositions bounding the hyphal protrusions. All of these aspects suggest that limitation of disease development in the resistant pepper may be due to the inhibition of the pathogen penetration, infection, invasion, and colonization by the defense structures such as root exudation materials, thickened middle lamellae, wound peridems and cell wall appositions.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.9 no.1
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• pp.114-128
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• 1996

"Chimumchang" seems to be a kind eczema. The cause and symptom of eczema is similar to that of chimumchang. so, I investigated the literatures of oriental medicin and recent medicine. The results are summarized as follows. 1. Chimumchang seems to be a kind of eczema. Allergic contact dermatitis, atopic dermatitis, seborrheic dermatits, neurodermatitis, nummular dermatitis, xerotic eczema and so on are similar to chimumchang on cause & symptom. 2. The cause of chimumchang are summer vital energy excess(夏脈太過), sehwa excess(歲火太過), herat meridian wind-heat(心經風熱), heart fire(心火), wind taken spleen dampnees(脾濕受風), and so on. The cause of eczema are contact of external agent, immunological problem, heridity, infection, abnormalities of sebaceous glands, neurologic dysfuction, and so on. 3. The symptoms of chimumchang have been described to the three pattern on literature of oriental medicine until now. 1) scratching slightly, It has exudation, and combination. 2) Vital energy on skin is irritable by psychological problem. At the acute stage the patient is complain of itching & pain, and gradually the exudation expands in whole body. 3) early peorid it's size is very small, and at first the patient is complain of itching, later complain of pain. The exudation change to ulcer and expands in the whole body. 4. In the investigation of prescription(Total: 34, Oral medicine: 4, Ointment: 30), the most frequently used oral medicine is sungmatang(4 times), and the most frequently used ointment are kohosan, korvunsan and kegwanhyuldobang.

• Korean Journal of Weed Science
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• v.15 no.2
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• pp.131-140
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• 1995

This experiment was conducted to test the effect on germination and seedling growth of major industrial crops and weed control potential using alfalfa plant. When dried alfalfa residues were mixed into vermiculite, germination, length of shoot and root of crops, such as Perilla frutescens, Sorghum nevosum, Platycodon grandiflorum and weeds, Digitaria saguinalis, Setaria viridis, Siegesbechia pubescens, Ammaranthus lividus, and Solanum nigrum, were significantly inhibited as the dried residue concentration increased. More than 10% concentration of the dired residue caused 80% germination and growth inhibition. The fresh alfalfa exudation also inhibited the germination and seedling growth of crop, barley, rye, alfalfa, and sesame, and weeds, Echinochloa crus-galli, Siegesbechia viridis, and Portulaca oleracea. The degree of inhibition showed the different response according to the fresh exudation concentration, types of crops and weeds. Generally, as the exudation concentration increased, the germination and seedling growth of crops and weeds inhibited. The exudation of dried residue also exhibited the strong inhibition effect on germination and seedling growth of crops, alfalfa, Platycodon grandiflorum, barley, sesame, rye and weeds, D. sanguinalis, S. pubescens, S. viridis, P. oleracea, E. crus-galli, At the 10% concentrations, S. pubescens, and P. oleracea were not germinated and showed only 15% germination in the S. viridis. From this study, would conclude that alfalfa plant contained water soluble phytotoxic substances which were inhibitory to weeds and crops. This results suggest that alfalfa had some possibility to control some weed species using toxic compounds like natural herbicide.

• Korean Journal Plant Pathology
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• v.14 no.1
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• pp.46-51
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• 1998

A method for inoculum production of Botryosphaerisa dothidea was developed using cucumber disks and cereal media. Disks of cucumber fruits, and cereal media of barley, wheat, and rice seeds were inoculated with mycelial plugs of B. dothidea and incubated at 27$^{\circ}C$. Pycnidia were produced on the surface of cucumber disks and seeds after 5 days of inoculation. When the inoculated barley seeds were immersed in sterilized distilled water for 5 minutes, abundant conidia of B. dothidea were exuded from mature pycnidia. Conidia were held together by mucilage as they were released from an ostiole. Compared with the conventional method for inoculum preparation using agar media, such as potato-dextrose agar and oatmeal agar, this method could minimize the tedious work required for inoculum preparation within a shorter period of time.

• FLOWER RESEARCH JOURNAL
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• v.17 no.4
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• pp.237-241
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• 2009

This study was conducted to determine injury symptoms of orchids by sulfur dioxide gases, three orchid plants (Phalaenopsis, Cymbidium, Oncidium) were exposed to sulfur dioxide gas in an enclosed growth chambers. Sulfur dioxide gases treatments consist of five different concentrations (0, 5, 10 25, and 50 ppm) and plant exposure of 18 hours with $25{\pm}5^{\circ}C$ air temperature and $50{\pm}5%$ relative humidity. SPAD values for chlorophyll content and percent leaf injury as well as leaf ion exudation were measured before and after the gas treatments. Phalaenopsis leaves showed 23.3% leaf injury at 10 ppm sulfur dioxide gas, whereas Cymbidium and Oncidium showed 4.0 and 4.4% leaf injury under 25 ppm or less, respectively. Major leaf injury symptoms appeared as initial water-soaking under side of the leaf, followed by rapidly progressed complete leaf discolorization or chlorosis. As the gas concentration increased, the SPAD value decreased while ion exudation increased. Cymbidium and Oncidium were resistant to sulfur dioxide gas than Phalaenopsis.

• Korean Journal of Soil Science and Fertilizer
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• v.30 no.3
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• pp.257-264
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• 1997

To find out the influence of different rice varieties on the emission of methane in the soil and the exudation of carbohydrates from root, the experiment was conducted on a fine silty, mesic family of Aeric Fluventinc Haplaquepts (Jeonbug series). The varities of rise involved one early maturing variety of Japonica type(namweon) and three mid-to-late maturing varieties of Japonica type and one mid-to-late variety of Indica${\times}$Japonica crossed. The methane flux over the rice canopy was measured according to the closed chamber method modified by Shin and the methane concentration in the soil was measured using porous cup installed in the soil. The carbohydrate exudates from root were measured under nutrient solution culture. It was found that the methane flux tended to be lower in early maturing varieties than in the mid-to-late maturing varieties, lower in indica${\times}$Japonica crossed variety than in Japonica type varieties. There was positive correlation between the number of tillers and the weight of roots of rice plant and methane flux. The correlation, however, tended to be greater between the weight of roots and methane flux. There was no significant difference in the concentration of methane in the soil under different varieties of rice, excepting one variety, Kehwa under which methane concentration was highest. In case of carbohydrate exudation, early maturing variety tended to be higher than other varieties, although the opposite was the case in methane flux.

• Toxicological Research
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• v.13 no.1_2
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• pp.1-21
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• 1997

염증반응이란 우리 신체에 외부 물질이 침입했을 경우 대처하는 정상적인 현상이다. 외부에서 물리, 화학, 생물학적 물질에 노출되었을 경우 혈관이 팽창하고, 비특이적 면역반응이 시작된다. Selectin, integrin, immunoglobulin superfamily와 같은 adhesion molecule에 의해 macrophage 및 neutrophil과 같은 leukocyte의 반응을 촉진하기 위해 exudation이 일어나고 침입 부위에 모여 hyperemia가 발생된다.(Govan 등, 1995)

• Korean Journal of Veterinary Pathology
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• v.5 no.2
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• pp.49-56
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• 2001

Ocular lesions induced in 40 specific-pathogen-free Marek's disease (MD) resistant chicks by inoculation at 1 day of age with very virulent strain of Marek's disease virus (WV) were pathologically examined. Grossly,24/40 (60%) chicks had white gel-like materials in the vitreous body, whereas thickening and discoloration of iris (gray eye) were not observed. Microscopically, characteristic ocular MD lesions were observed in choroid (27/40), ciliary (30/40) and iris (23/40) in which small focal inflammatory to diffuse neoplastic Iymphoid cells were infiltrated. Five out of 40 MDV-inoculated birds revealed necrotizing Iymphomas in choroid. These lesions consisted of necrotic and degenerating Iymphoblasts accompanied by intranuclear inclusion body. There was retinal atrophy and necrosis with inclusion body detected in necrotic ganglion, inner or outer nuclear and infiltrated Iymphoblast cells. Conjunctiva showed lymphoid cell infiltration in 29/40 chicks inoculated with MDV, Vitreous body exhibited mild to severe exudation of eosinophilic proteinaceous material in 24/40 chicks. These lesions were associated with Iymphoid cell infutration, edema and fibrosis of choroid. Pecten (7/40) and optic nerve (13/40) were infiltrated usually mildly with Iymphoid cells. From these results, very virulent strain, Md/5 of MDV caused high incidence of ocular lesions in MD resistant chicks. In addition, Md/5 induced exudation of proteinaceous material into the vitreous body and fibrosis of choroid. Necrotizing ocular Iymphoma lesions in choroid is the first report in the MD literature.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.27 no.4
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• pp.243-250
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• 2014

Objective : The purpose of this report is to know the effect of oriental medical treatments on acute allergic contact dermatitis with multiple nummular eczema. Methods : We treated a 34-years-old woman patient with acute allergic contact dermatitis on face and multiple nummular eczema on both arm, right ankle and left inguinal region by Korean medicine. After Korean medical treatment for 42 days in hospital, we measured the decrease of exudation and pruritis as well as recovery of skin damages. We recorded pictures of changes on symptoms. Results & Conclusion : The symptoms of ACD and nummular eczema were significantly improved except scars. Exudation and pruritis were disappeared and skin damages were almost recovered until discharge from hospital. After 20 days we could confirm the symptoms were clearly disappeared. Thus Korean medical treatments are effective on ACD and nummular eczema.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.4
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• pp.280-291
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• 2007

Much research has been conducted in the field of phytoremediation since the discovery of the range of plants known as hyperaccumulators. Research has focused simultaneously on elucidating the mechanism of metal(loid) accumulation and development of practical techniques to enhance accumulation efficiency. To date, it is generally understood that there are five specific mechanisms employed by hyperaccumulating plant species that are either not or under utilized by non-hyperaccumulators. These include 1) enhanced metal(loid)s uptake through the root cell, 2) enhanced translocation in plant tissue, 3) detoxification and sequestration, 4) enhanced metal availability in soil:root interface, and 5) active root foraging toward metal(loid) enriched soils. Among these mechanisms, understanding of the plant-root effect on metal(loid) dynamics and subsequent plant uptake is vital to overcome the inherit limitation of phytoremediation caused by low metal(loid) solubility in soils. Plant roots can influence the soil chemistry in the rhizosphere through changes in pH and exudation of organic compounds such as low-molecular-weight organic acids (LMWOAs) which consequently change metal(loid) solubility. The decrease in soil pH by plant release of $H^+$ results in increased metal solubility. Elevated levels of organic compounds in response to high metal soil concentrations by plant exudation may also increases metal concentration in soil solution through formation of organometallic complexes.