• Title/Summary/Keyword: external stimulation

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Nitric oxide에 의한 수퇘지 음경후인근의 비아드레날린 비콜린 동작성 이완 I. 돼지 음경후인근의 비아드레날린 비콜린성 이완을 매개하는 신경전달물질 : nitric oxide와 vasoactive intestinal polypeptide (Nitric oxide(NO) mediating non-adrenergic non-cholinergic(NANC) relaxation in the boar retractor penis muscle I. Mediators of nonadrenergic, noncholinergic relaxation of porcine retractor penis muscle : nitric oxide and vasoactive intestinal polypeptide)

  • 문규환;김점용;김태완;강동묵;양일석
    • 대한수의학회지
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    • 제35권3호
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    • pp.447-458
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    • 1995
  • This study was carried out to characterize nonadrenergic, noncholinergic(NANC) relaxation of porcine retractor penis(PRP) muscle induced by electrical field stimulation(EFS) and to investigate the actions of niric oxide(NO) and vasoactive intestinal polypeptide(VIP) as candidates for NANC neurotransmitters. Biphasic relaxations of PRP muscle were induced by EFS to NANC nerve. Rapid-phase relaxation was observed at low frequency(0.5-16Hz) and slow-phase relaxation followed during high frequency(8-60Hz). Both relaxations were frequency-dependent and TTX($1{\times}10^{-6}M$)-sensitive. L-NAME($2{\times}10^{-5}M$) inhibited the rapid-phase relaxation, but not the slow-phase relaxation. The inhibition of the rapid-phase relaxation with L-NAME was reversed by L-arginine ($1{\times}10^{-3}M$) but not by D-arginine($1{\times}10^{-3}M$). Methylene blue($4{\times}10^{-5}M$) reduced the rapid-phase relaxation. Exogenous No(ExoNO, $1{\times}10^{-5}-1{\times}10^{-4}M$) induced dose-dependent relaxations of PRP muscle. Oxyhemoglobin($5{\times}1^{-5}M$) blocked the relaxation induced by ExoNO and inhibited EFS-induced relaxation. Hydroquinone($1{\times}10^{-4}M$) also abolished the relaxation induced by ExoNO, but did not affect EFS-induced relaxation. L-NAME resistant slow-phase relaxation to EFS was inhibited by ${\alpha}$-chymotrypsin(2.5 U/ml). Both methylene blue($4{\times}10^{-5}M$) and Nethylmaleimide($1{\times}10^{-4}M$) reduced the slow-phase relaxation by EFS. [4-Cl-D-$Phe^6$, $Leu^{17}$]-VIP($3{\times}10^{-6}M$) inhibited the slow-phase relaxation by EFS. External applications of VIP ($1{\times}10^{-7}M$) caused relaxations that were simillar to the L-NAME resistant slow-phase relaxations induced by EFS, and relaxant effects of exogenous VIP were blocked by ${\alpha}$-chymotrypsin(2.5 U/ml).

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Inhibiyory Effects of Ginseng Saponins Metabolized in Degestive Tract on Adrenal Secretion of Catecholamines In vitro

  • Tachikawa Eiichi;Hasegawa Hideo;Kenzo Kudo;Kashimoto Takeshi;Miyate Yoshikazu;Kakizaki Atsushi;Takahashi Katsuo;Takahashi Eiji
    • 고려인삼학회:학술대회논문집
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    • 고려인삼학회 2002년도 학술대회지
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    • pp.392-400
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    • 2002
  • We have previously found that the saponins but not other components in the ginseng reduce the secretion of catecholamines (CAs) from bovine adrenal chromaffin cells, a model of sympathetic nerves, evoked by acetylcholine (ACh) due to the blockade of $Na^+$ influx through nicotinic ACh receptor-operated cation channels, and it has been concluded that the inhibitory effect may be associated with the anti-stress action of ginseng. However, the saponins, which showed the great reduction of the CA secretion, were mainly the protopanaxiatriols. The protopanaxadiol and oleanolic acid saponins had a little or little such effect. Recent studies demonstrated that the oligosaccharides connected to the hydroxyl groups of the aglycones of the saponins are in turn hydrolyzed by gastric acid and enzymes in the intestinal bacteria when the ginseng is orally administrated. In this study, the effects of their major 6 kinds of metabolites on the secretion of CAs were investigated. All metabolites (M1, 2, 3 and 5 derived from the protopanaxadiols, and M4 and 11 from the protopanaxiatriols) reduced the ACh-evoked secretion from the cells. In the metabolites, the M4 inhibition was the most potent ($IC_{50}({\mu}M):M4(9)$ < M2 (18) < M3 (19) < M1l (22) < M5 (36) < MI (38)). Although M4 also reduced the CA secretion induced by high $K^+$, a stimulation activating voltage-sensitive $Ca^{2+}$ channels, the inhibitory effect was much less than that on the ACh-evoked secretion. M4 inhibited the ACh-induced $Na^+$ influx into the cells in a concentration-dependent manner similar to that of the inhibition of the ACh-evoked secretion. When the cells were washed by the incubation buffer after the preincubation of the cells with M4 and then incubated without M4 in the presence of ACh, the M4 inhibition was not completely abolished. On the other hand, its inhibition was maintained even by increasing the external ACh concentration. These results indicate that the saponins are metabolized to the more active substances in the digestive tract and the metabolites attenuate the secretion of CAs from bovine adrenal chromaffin cells stimulated by ACh due to the noncompetitive blockade of the ACh-induced $Na^+$ influx into the cells. These findings may further explain the anti-stress action of ginseng.

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퇴계와 율곡의 인심도심설 - 주자 심론의 한국적 전개 - (Toegye and Yulgok's Theory of Human Mind·Moral Mind - The Korean Development of Chutzu's Theory of Mind)

  • 전현희
    • 한국철학논집
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    • 제41호
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    • pp.93-127
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    • 2014
  • 퇴계와 율곡은 모두 현실 감정으로부터 선의 지평을 확보하고자 하였다. 퇴계는 선의 지평이 이치에 의해 마련된다고 보았으며, 율곡은 그것이 기운에 의해 마련된다고 생각했다. 그런데 이치에 의해 마련한 선의 지평으로서의 사단은 인간의 주체성을 위협할 수 있었으며, 기운에 의해 확보된 선의 지평으로서의 중절한 감정은 그 중절의 여부가 자체로 증명되지 않았다. 그들은 이러한 문제점을 인심도심설을 통해 풀어간다. 퇴계에게 도심은 인간을 도덕적 주체로 서게 하는 것으로서의 의미를 지니며, 율곡에게 도심은 중절의 기준을 만족시키는 것으로서의 의미를 지닌다. 반면 주자 인심도심설에 의하면 인심도심은 지각하는 마음으로서, 미발과 이발을 주재하여 중화中和를 이루는 역할을 하는 것이다. 즉 이발에 한정되는 마음이 아니다. 그렇다면 퇴계와 율곡의 인심도심설은 그들 고유의 철학적 문제의식을 통해 새롭게 전개된 결과라고 보아야 할 것이다. 퇴계와 율곡의 인심도심설은 의지의 역할을 강조함으로써 마음의 선을 실질적 선행으로 이끄는 계기를 마련하였으며, 아울러 인간을 도덕적 주체로 서게 하기 위한 공부론의 토대를 경험 가능한 심리적 층차에 마련했다는 점에서 구체적이고 현실적인 마음이론을 완성했다는 의의를 지닌 것으로 평가할 수 있다.

COMPAREX 의 항염 및 진정 화장품 소재로서의 연구 (A Study on Anti-inflammatory and Skin Soothing Cosmetics Material of COMPAREX)

  • 김하연;이재석;이소헌;김예향;정은영;이강혁;신송석
    • 대한화장품학회지
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    • 제49권3호
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    • pp.269-276
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    • 2023
  • 최근 다양한 외부 요인에 의해 민감해진 피부를 진정시키기 위해 더욱 안전하고 효과적인 진정 소재 개발에 대한 관심이 증가하고 있다. 따라서 본 연구에서는 항염 효능으로 잘 알려진 마치현 추출물을 기반으로 애엽, 홍경천 추출물을 혼합함으로써 COMPAREX를 제조하고, 피부 진정 효능에 미치는 영향을 마치현 단일 추출물과 비교하였다. 실험 결과, COMPAREX는 마치현 추출물 보다 LPS에 의해 유도된 NO 생성을 더욱 억제시키고, 염증성 인자인 iNOS, COX-2, TNF-α, IL-6의 발현을 현저히 억제시킴으로써 염증완화 효능이 더 효과적인 것을 확인하였다. 또한, COMPAREX는 마치현 추출물 보다 SDS 자극에 대해 IL-1α의 유전자 및 단백질 발현을 더욱 억제시킴으로써 진정 효능이 더 효과적인 것을 확인하였다. 추가적으로, 모발세포에서 COMPAREX는 PM2.5에 의해 증가된 COX-2, IL-8의 발현을 억제시켜 두피 염증 완화 효능이 있으며, carbonylated protein을 저해시킴으로써 외부 자극으로부터 손상된 두피를 보호할 수 있는 효능을 확인하였다. 따라서, COMPAREX는 마치현 단일 추출물 보다 개선된 천연 진정 소재로의 활용 가능성을 보였고, 향후 더마 코스메틱 소재로 활용될 수 있을 것으로 기대된다.

A Study on the Interior Design of a Dog-Friendly Hotel Using Deepfake DID for Alleviation of Pet loss Syndrome

  • Hwang, Sungi;Ryu, Gihwan
    • International Journal of Advanced Culture Technology
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    • 제10권1호
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    • pp.248-252
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    • 2022
  • The environment refers to what is surrounded by something during human life. This environment is related to the way humans live, and presents various problems on how to perceive the surrounding environment and how the behaviors that constitute the environment support the elements necessary for human life. Humans have an interest in the supportability of the environment as the interrelationship increases as humans perceive and understand the environment and accept the factors supported by the environment. In space, human movement starts from one space to the next and exchanges stimuli and reactions with the environment until reaching a target point. These human movements start with subjective judgment and during gait movement, the spatial environment surrounding humans becomes a collection of information necessary for humans and gives stimulation. will do. In this process, in particular, humans move along the movement path through movement in space and go through displacement perception and psychological changes, and recognize a series of spatial continuity. An image of thinking is formed[1]. In this process, spatial experience is perceived through the process of filtering by the senses in the real space, and the result of cognition is added through the process of subjective change accompanied by memory and knowledge, resulting in human movement. As such, the spatial search behavior begins with a series of perceptual and cognitive behaviors that arise in the process of human beings trying to read meaning from objects in the environment. Here, cognition includes the psychological process of sorting out and judging what the information is in the process of reading the meaning of the external environment, conditions, and material composition, and perception is the process of accepting information as the first step. It can be said to be the cognitive ability to read the meaning of the environment given to humans. Therefore, if we can grasp the perception of space while moving and human behavior as a response to perception, it will be possible to predict how to grasp it from a human point of view in a space that does not exist. Modern people have the theme of reminiscing dog-friendly hotels for the healing of petloss syndrome, and this thesis attempts to approach the life of companions.

공동주택에서 치유조경계획을 위한 가이드라인 연구 (A Study on the Design Guidelines of Healing Landscape in Housing Complexes)

  • 천현우;이시영
    • 한국조경학회지
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    • 제44권5호
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    • pp.26-37
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    • 2016
  • 본 연구는 공동주택단지를 대상으로 하여 외부의 조경공간을 통해 치유환경을 제공하여 치유기능을 높이기 위한 조경계획방안을 제시하고자 한다. 본 연구를 통해 지속적으로 늘어나고 있는 집합주거공간의 외부공간 조성에 있어 거주민들에게 생활환경을 높일 수 있는 기회와 함께 조경설계기법의 대안적인 기준을 마련한다는 점에서, 정형화되어 있지 못하고 있는 조경설계 분야의 새로운 가이드라인을 제공하는데 그 의미가 있다. 연구 방법으로는 기존 학자들의 치유정원에 관한 특성을 분석하여 공통적인 환경특성 요소들을 도출하고, 이를 바탕으로 기존 국내 외 문헌을 통하여 세부항목을 설정하며, 선행연구와 사례조사, 설문분석을 통해 치유조경계획 요소를 도출하였다. 치유환경의 기능적 계획요소 6가지 항목을 설정하였다. 안전한 환경, 치료적 환경, 보조적 환경, 지남력 강화환경, 쾌적성, 사회적 환경으로 구분하였고, 공간계획요소는 국내 외 사례고찰로 병원, 요양원, 숲 등을 대상으로 하여 육체적, 정신적 안정에 도움을 주기 위한 공간 및 시설을 도입하고 있는 것을 알 수 있었다. 계획요소로 공간계획, 동선계획, 포장계획, 수경계획, 식재계획, 시설계획, 프로그램으로 구분하여 계획요소를 도출하였다. 도출된 항목을 외부공간의 단지출입 영역, 내 외부 전이영역, 외부활동 영역, 테마별 공간영역으로 구분하고, 계획 요소로 공간계획에는 자연속 산책이 가능하고, 범죄로부터 안전, 다양한 휴게시설이 있도록 계획한다. 식재계획으로는 계절감을 느낄 수 있는 수목 식재, 촉각 미각, 후각을 자극하는 식물 식재, 풍부하고 다양한 식물 식재로 계획한다. 동선계획은 외부로부터 동선을 분리하고, 건강증진을 위한 산책, 지압로, 흙길 등 자연감상이 가능한 동선으로 계획한다. 포장계획은 위험에 쉽게 인식할 수 있고, 인공재료보다는 자연형 재료로 계획한다. 시설물계획은 취약계층을 위한 안내표, 강설시에도 이용할 수 있도록 쉘터 및 이동 가능한 벤치를 계획한다. 수 공간계획은 잔잔한 물 공간을 조망하는 사색 가능한 물시설과 분수 등 깔끔하고 도식적인 물시설로 계획한다. 치유프로그램으로는 휴식 명상을 취할 수 있으며, 요가, 산책 등 가벼운 운동을 위한 프로그램을 계획한다. 치유조경계획 요소를 도출하여 가이드라인을 제시하였으나, 계획요소와 계획내용들이 치유기능을 향상시키는데 효과가 있을지에 대한 정성적, 정량적 평가가 제한되어 이에 대한 연구가 추후 이루어질 필요가 있다.

수축력과 신장력을 병용한 골신장술에서의 다양한 힘의 비와 부여시기에 따른 신연골 반응 (THE REACTION OF BONE REGENERATE TO THE VARIOUS FORCE RATIO AND PERIODS ON DISTRACTION OSTEOGENESIS WITH COMBINED DISTRACTION FORCE AND COMPRESSION FORCE)

  • 김욱규;신상훈;정인교;김철훈;허준;윤일
    • Maxillofacial Plastic and Reconstructive Surgery
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    • 제27권5호
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    • pp.403-414
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    • 2005
  • The purpose of this study was to identify the effectiveness of the modified distraction osteogenesis (DO) method with the concept of overdistraction and compression stimulation which have been previously suggested by the authors in 2002 and to explore the optimal distraction-compression ratio and appropriate latency period for the compression force application during consolidation. The experimental specimens were assessed with radiography, histologic findings, and dual energy x-ray absorptiometry (DEXA) after the conventional DO method and the modified DO technique had been applied on rat mandibles. Total 60 rats were used for the study. In experimental group of 54 adult rats, mandibular osteotomies between the first and second molar areas were performed and customized external distractors were applied. The surgeries on 6 rats of control group also were done with same osteotomy technique and DO device application. Final amount of distraction was set-up as 2 mm on both groups. But, in a experimental group of 54 rats, distraction osteogenesis with a compression force were performed with the different distraction-compression ratio and variant latency periods for compression. The three ratio-subgroups were made as distraction 4 mm group with compression 2 mm, distraction 3 mm group with compression 1 mm, and distraction 2.5 mm group with compression 0.5 mm. In addition, The three subgroups with 3, 7, 11 days latency period prior compression were allocated on each ratio-subgroups. Total 9 subgroups consisted of 6 rats on each subgroup. In control group of 6 rats, conventional distraction technique were routinely performed. The rats of control groups were sacrificed on postoperative 3, 6 weeks after 2 mm distraction. The rats in the experimental groups also were sacrificed on the same euthanasia days of control groups to compare the wound healing. Final available specimens were 55 rats except 5 due to osteomyelitis, device dislodgement. Distraction-compression combined group on 6 weeks generally had showed increased bone mineral density than the same period group of conventional distraction technique on the DEXA study. More matured lamellar bone state and extended trabecular pattern in the combined group than those of control group were also observed in the histologic findings on 6 weeks. In the distraction-compression combined groups, the bone density of 2.5 mm distraction subgroups with 0.5 mm compression showed the highest value on the DEXA study among various force ratio groups. In the distraction-compression combined groups, the bone density of 3 day latency period subgroups showed the highest value on the DEXA study among various latency period groups for the compression application. From this study, we could deduce that 1/5 force ratio for the compression versus distraction, 3 day latency period prior compression application would be the most effective condition if modified distraction osteogenesis technique might be applicable. The modified DO method with a compression force may improve the quality of bone regenerate and shorten total treatment period in comparison with conventional DO technique clinically.