• 제목/요약/키워드: extension of the L

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Interspecific Hybridization between Populus caspica L. × P. deltoids L 62/154 Using in vitro Embryo Development and Germination

  • Ali, Jafari mofidabadi;Mansooreh, Kamandloo;Hamid, Selamti
    • Journal of Forest and Environmental Science
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    • 제33권3호
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    • pp.197-201
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    • 2017
  • Populus. caspica L. is an Iranian indigenous poplar species which naturally distributed in the northern part of country. Unfortunately, overuse has removed many of the stems of better form, so that natural stands now usually appear small and crook. Therefore genetic variation for selection of new superior clone of this species is needed. Conventional hybridization system is currently used to induce genetic variation in poplar species but incompatibility barriers have been observed between them. In vitro ovule embryo culture was used to overcome incompatibility obstacle for interspecific hybridization between Populus caspica L. with Populus deltoids L.62/75. Female flowers of Populus caspica L. have artificially been pollinated with pollen grain of P. deltoides 62/75 in one direction using twig and pot crossing system. Ovaries at different ages (7, 14 and 21 days after pollination) were disinfected through 70% ethanol for 1 minute, 5% of sodium-hypochlorite solution for fifteen min followed by three time rising with sterile distil-water. Isolated ovaries were then transferred to MS hormone free medium containing 30 and 60 g/L sucrose for embryo development and germination. Collected data have been analyzed by two factorial experimental designs. The results indicated that there were significant differences between age of embryos for development and germination at ${\alpha}=0.01%$. Highest embryo germination (45%) was observed from 21 days old ovaries. No significant differences were observed between MS culture media containing 30 and 60 g/L for percentages of ovary-embryo germination and number of germinated embryo per ovary at ${\alpha}=0.05%$. Fourteen percentage of embryo germination obtained in MS medium supplemented with 60 g/L sucrose, while only 35% of isolated ovaries were able to germinate in MS containing 30 g/L sucrose. Induced plantlets in 4 cm height were transferred into pots containing soilless (1:1:1 peat, per lit and vermiculite) medium for acclimatization. After successful acclimatization, plants were delivered to nursery.

시판 효모의 종류를 달리하여 제조한 '청향' 와인의 품질 특성 (Comparison of Quality Characteristics of 'Cheonghyang' Wine fermented with Different Commercial Yeasts)

  • 이효영;이하연;권혜정;박지선;안문섭;정석태;이재형
    • 동아시아식생활학회지
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    • 제26권6호
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    • pp.543-549
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    • 2016
  • The objective of this study was to investigate quality properties of 'Cheonghyang' wine using different commercial yeast strains. Soluble solid content, pH level and total acidity of 'Cheonghyang' grape were $20^{\circ}Bx$, pH 3.5 and 0.66%, respectively. Total acidity ranged from 0.91~1.06% in the middle stage of fermentation and decreased to 0.77~0.82% when alcoholic fermentation finished. Alcohol content in wines ranged from 12.5% to 12.9% showing no significant difference in yeast strains. Wine fermented with Red Fruit had high volatile acid content (189.0 mg/L) whereas wine fermented with Fermivin indicated low volatile acid content (77.7 mg/L). Wines made with Montrachet, Fermivin and Aroma White had low brightness (L-value) compared to others. Results from sensory evaluation demonstrated that commercial wine yeasts, Montrachet and Fermivin, can be applied to improve sensory properties of 'Cheonghyang' wine such as aroma, acidity and transperency. On the other hand, preferences of wine fermented with EC-1118 strain containing lots of tannins and total polyphenols were significantly reduced.

당화방법에 따른 유산균 발효천마의 품질특성 (Quality Characteristics of Fermentation Gastrodia elata Blume by Saccharifying Methods)

  • 송영은;최소라;송은주;서상영;이인석;한현아;이기권;송영주;김영회;김명곤;박신영
    • 한국식품영양학회지
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    • 제29권5호
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    • pp.698-705
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    • 2016
  • 천마의 불쾌취 개선을 위한 당화방법별 유산균 발효 결과는 3일 발효시 엿기름과 쌀누룩 이용 당화에서 총 산도가 각각 2.23, 2.33%이었으며, 생균수는 9.14 log cfu/mL와 9.27 log cfu/mL로 높았다. 발효기간이 길어짐에 따라 총 산도는 계속 높아져 3.35%까지 증가하였으나, 생균수는 3일 발효에서 가장 많았다. 아미노산 함량은 엿기름과 쌀누룩 이용 당화에서 높았고, 유리당 함량은 효소 이용 당화에서 높았으며, 그 중 glucose 함량이 가장 높았다. 천마 주성분인 gastrodin, p-HBA 등 주 성분 함량도 효소 이용 당화에서 가장 높았다. 이취성분인 p-cresol 함량이 엿기름 이용 당화에서 낮아 관능평가 결과에서도 엿기름을 이용하여 당화한 유산균 발효천마가 가장 좋은 기호도를 나타내었다.

Responses of Young 'Fuyu' Persimmon Trees to Summer Fertilization Rate and Leaf-fruit Ratio

  • Choi, Seong-Tae;Kim, Seong-Cheol;Ahn, Gwang-Hwan;Park, Doo-Sang;Kim, Eun-Seok;Choi, Jae-Hyeok
    • 한국토양비료학회지
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    • 제49권5호
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    • pp.577-583
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    • 2016
  • Small-sized persimmons produced by high crop load are better accepted in the export markets. However, maintaining high crop load frequently results in weakness of tree vigor, deterioration of fruit quality, and increase of the risks for alternate bearing. This experiment was conducted to determine the combined effects of fertilization rate and leaf-fruit (L/F) ratio on container-grown 3-year-old 'Fuyu' persimmon trees. Application of 3.6-g N, 2.1-g $P_2O_5$, 2.7-g $K_2O$, 2.7-g CaO, and 0.6-g MgO was for the control fertilization rate (CF) and that of a 3-fold CF was for the high fertilization rate (HF). Commercial fertilizers were surface-applied to a container on July 6, July 17, and August 10 in three equal aliquots. Single tree for each fertilization rate was assigned for 12 L/F ratios (5, 6.3, 7.7, 9, 10.4, 13, 15.5, 18, 21, 24, 27, and 33) mostly by fruit thinning or rarely by defoliation on July 1. HF did not affect the yield, weight and soluble solids of the fruits but decreased skin color. As L/F ratio increased, yield decreased but average weight, skin color, and soluble solids of fruits increased. With HF, N and K concentrations in leaves, fruits, and shoots increased to some extent but soluble sugars in dormant shoots decreased. Many shoots were cold-injured with low L/F ratio especially at the HF. HF did not increase number of flower buds the next spring either on a shoot or on a tree basis but increased shoot length, compared with the CF. Increasing L/F ratio markedly increased number of flower buds and shoot growth the following year at both fertilization rates. Therefore, an appropriate combination of fertilization rate and L/F ratio should be necessary to maintain stable fruit production and tree vigor at high crop load.

강황지하부 부산물에서 분리한 Quercetin-3-O-${\beta}$-D-glucopyranoside-7-O-${\alpha}$-L-rhamnopyranoside가 선충의 수명연장에 미치는 영향 (Lifespan Extension Property of Quercetin-3-O-${\beta}$-D-glucopyranoside-7-O-${\alpha}$-L-rhamnopyranoside from Curcuma longa L. In Caenorhabditis elegans)

  • 안달래;이은별;김반지;이소연;안민실;은재순;신태용;김대근
    • 생약학회지
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    • 제45권4호
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    • pp.275-281
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    • 2014
  • After harvesting the medicinal parts of Curcuma longa, the remaining underground parts were discarded. From the remaining underground parts of Curcuma longa quercetin-3-O-${\beta}$-D-glucopyranoside-7-O-${\alpha}$-L-rhamnopyranoside (Q37) was isolated. The antioxidant activities in vitro and lifespan-extension effect of Q37 were elucidated using the Caenorhabditis elegans. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging effect of Q37 showed similar potent activities in comparison with vitamin C. Q37 also showed potent superoxide quenching activities as measured by the riboflavin- and xanthine-originated superoxide quenching activity tests. Q37 prolonged lifespan of worms under normal culture condition. In terms of protective effect of Q37 on the stress conditions such as thermal and oxidative stresses, Q37-treated worms exhibited enhanced survival rate, as compared to control worms. To know the possible mechanism of Q37-mediated increased lifespan and stress resistance of worms, we examined the activities of Q37on superoxide dismutase (SOD), and invested intracellular reactive oxygen species (ROS) levels. The results revealed that Q37 was able to elevate SOD activity of worms and reduce intracellular ROS accumulation in a dose-dependent manner.

블루베리 '듀크' 품종의 양액재배 시 수체 생장과 수량 반응 (Growth and Yield Response of Highbush Blueberry 'Duke' to Hydroponic Cultivation)

  • 천미건;이서현;박경미;최성태;황연현;장영호;김진국
    • 생물환경조절학회지
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    • 제30권3호
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    • pp.244-249
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    • 2021
  • 본 시험은 국내에 가장 많이 보급되어있는 북부하이부쉬 블루베리 '듀크' 품종에 대한 시설하우스 양액재배 가능성을 평가하기 위해 실시하였다. 블루베리 용기재배에 보편적으로 활용되는 피트모스(130L)와 펄라이트(40L) 배지를 180L 플라스틱 용기에 혼합하여 1년생 묘목을 심은 후 8년간 양액을 지속적으로 공급하는 양액처리구와 지하수만 공급하는 무처리구를 비교하였다. 양액은 NO3-N 4.6, NH4-N 3.4, PO4-P 3.3, K 3, Ca 4.6, Mg 2.2mmol-1를 EC 1.5로 조절하여 공급하였다. 양액처리구는 처리 후 8년차까지 수체 생장이 양호하였으며, 무처리구보다 주당 신초수가 18% 많고 주당 총신초장은 24%가 길었다. 양액처리구는 무처리구에 비해 뿌리 발달이 양호하였으며 주당 총건물중은 1.4배로 컸다. 식물체의 잎, 신초, 묵은 가지의 무기원소나 유기화합물이 양액처리구에서 대체로 높게 나타나 양액을 통한 양분흡수가 원활하였음을 확인되었다. 수량은 양액처리구에서 4년생 때부터 성과기에 달하여 시험이 종료될 때까지 높게 유지되었다. 이와 같은 결과로 양액재배기술은 블루베리 재배에 유용하게 활용 가능할 것으로 평가되었다.