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Clonal Propagation through Leaf Sheath Culture of Phalaenopsis

  • Jo, Man-Hyun;Ham, In-Ki;Lee, Mi-Ae;Han, Gyu-Heung;Woo, In-Shik
    • Plant Resources
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    • v.5 no.3
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    • pp.176-180
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    • 2002
  • This study was conducted to develop the clonal propagation technique through in vitro culture using by leaf sheath explants of Phalaenopsis grown in vitro. The highest frequency of protocorm-like body (PLB) formation was obtained when explants of leaf sheath were cultured on VW medium containing 30g/L sucrose, 500 mg/L activated charcoal, 150 mVL coconut water, 1 mg/L NAA, 1 mg/L 2ip and 2.5 g/L gelrite. The PLB formation rate of VW medium was highest followed by modified Hyponex medium, and lowest in MS medium. Plantlets induced from PLBs transferred to plastic pots including spagnum moss were well developed.

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Plant Regeneration from Leaf Segments Culture of Several Jeju Native Lilies (잎절편 배양에 의한 제주 자생나리의 재분화)

  • Kim Jeong-Seon;Song Seung-Woon;Kim Yong Chol;Kim Kwang-Ho;Park Young-Chul
    • Korean Journal of Plant Resources
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    • v.18 no.3
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    • pp.450-455
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    • 2005
  • This study was conducted to examine the effects of plant growth regulators on the plant regeneration from leaf segments of Lilium callosum, L. concolor var. partheneion, and L. formosanum. Leaf segments were sectioned about 5 mm long and cultured on the basal medium (MS medium with $3\%$ sucrose and $0.8\%$ agar) under dark condition, The most effective plant regulators on harvesting more shoots from leaf culture of L. callosum were $0.2\;mg{\cdot}L^{-1}\;BA$ and $0.5\;mg{\cdot}L^{-1}\;NAA$. Culturing in the basal medium with $0.2\;mg{\cdot}L^{-1}\;BA$ and $2.0\;mg{\cdot}L^{-1}\;NAA$ was effective for leaf culture of L. concolor var. partheneion. The treatment of $1.0\;mg{\cdot}L^{-1}\;BA$ and $1.0\;mg{\cdot}L^{-1}\;NAA$ was the most effective condition for shoot harvest at the leaf culture of L. formosanum.

Mycelial Yield of Pleurotus ostreatus Using Thinned Apple, Pear, and Peach on Submerged Culture

  • Jung, Gi-Tai;Ju, In-Ok;Yu, Young-Zin;Jeong Ryu;Park, Joung-Sik;Park, Yeong-Geun
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.8 no.5
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    • pp.286-290
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    • 2003
  • The effect of thinned fruits, apple, pear and peach, on the mycelial growth of mushrooms was investigated. The growth of mycelia with the addition of thinned fruit was clearly better than that in the control for all the tested mushrooms. The growth rate of Pleurotus ostreatus was faster than any other mushroom. The optimal concentrations of thinned apple, pear, and peach in a solid culture were 1.0%, 1.0%, and 3%, respectively, while in a liquid culture the optimal concentrations were 5,0%, 3.0%, and 5.0%, respectively. When Pleurotus ostreatus was incubated in a 20-L pilot scale fermenter with 10 L of a liquid medium containing 3% thinned fruit at 25$^{\circ}C$ and 6 vvm for 10 days, the mass-production of mycelia was 74.2 g/10 L (apple), 96.2 g/10 L (pear), and 86.3 g/10 L (peach). The mycelial yield of Pleurotus ostreatus in a medium containing thinned fruit was 2 ∼ 3 times higher than that in the control.

Damages of Young Persimmon Tree as Affected by Application of Immature Liquid Pig Manure

  • Choi, Seong-Tae;Park, Yeo-Ok;Ahn, Gwang-Hwan;Kim, Eun-Gyeong;Son, Ji-Young;Joung, Wan-Kyu;Hong, Kwang-Pyo
    • Korean Journal of Environmental Agriculture
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    • v.38 no.2
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    • pp.89-95
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    • 2019
  • BACKGROUND: Liquid pig manure (LPM) has been used as an alternative for conventional fertilizers on some gramineous crops. However, its chemical properties varied widely depending on the degree of the digestion. A pot experiment was conducted to determine the responses of persimmon trees to immature (not well-digested) LPM application. METHODS AND RESULTS: Ten application levels of immature LPM, consisted of a total of 3 to 30 L in 3-L increment, were applied during summer to 5-year-old 'Fuyu' trees grown in 50-L pots. Increasing the LPM application rate caused defoliation, wilting, and chlorosis in leaves. When applied with the rate of 3 L during summer, the tree produced small fruits with low soluble solids and bore few flower buds the following season, indicating insufficient nutritional status. In trees applied with the LPM rates of 6~12 L, both fruit characteristics and above-ground growth of the trees appeared normal but some roots were injured. However, application of higher LPM rates than 27 L resulted in small size, poor coloration, or flesh softening of the fruits the current season. Furthermore, the high LPM rates caused severe cold injury in shoots during winter and weak shoot growth the following season. It was noted that the application of higher LPM rate than 9 L damaged the root, even though above-ground parts of the tree appeared to grow normally. CONCLUSION: The results indicated that an excessive immature LPM application could cause various injuries on leaves, fruits, and the roots in both the current and the following season.

A Study on the performance improvement of L-Expressnet added slot-extension function (슬롯확장기능을 갖는 L-Expressnet의 성능 향상에 관한 연구)

  • 유동관
    • Journal of the Korea Society of Computer and Information
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    • v.5 no.4
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    • pp.76-81
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    • 2000
  • In this Paper, an improved L-Expressnet Protocol is proposed by supplementing slot-extension function. This protocol is Proposed to complement the shortcomings of the conventional protocol which is used for the medium access control (MAC) in LAN. We analyzed the protocol in channel utilization viewpoint and compared the result with that of the conventional L-Expressnet medium access control (MAC) protocol. From this result. we showed that the channel utilization of the improved L-Expressnet protocol added slot-extension function is superior to that of the conventional Protocol.

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