• Title/Summary/Keyword: expression of reliability

Search Result 185, Processing Time 0.029 seconds

Comprehensive analysis of miRNAs, lncRNAs and mRNAs profiles in backfat tissue between Daweizi and Yorkshire pigs

  • Chen Chen;Yitong Chang;Yuan Deng;Qingming Cui;Yingying Liu;Huali Li;Huibo Ren;Ji Zhu;Qi Liu;Yinglin Peng
    • Animal Bioscience
    • /
    • v.36 no.3
    • /
    • pp.404-416
    • /
    • 2023
  • Objective: Daweizi (DWZ) is a famous indigenous pig breed in China and characterized by tender meat and high fat percentage. However, the expression profiles and functions of transcripts in DWZ pigs is still in infancy. The object of this study was to depict the transcript profiles in DWZ pigs and screen the potential pathway influence adipogenesis and fat deposition, Methods: Histological analysis of backfat tissue was firstly performed between DWZ and lean-type Yorkshire pigs, and then RNA sequencing technology was utilized to explore miRNAs, lncRNAs and mRNAs profiles in backfat tissue. 18 differentially expressed (DE) transcripts were randomly selected for quantitative real-time polymerase chain reaction (QPCR) to validate the reliability of the sequencing results. Finally, gene ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) enrichment analysis were conducted to investigate the potential pathways influence adipocyte differentiation, adipogenesis and lipid metabolism, and a schematic model was further proposed. Results: A total of 1,625 differentially expressed transcripts were identified in DWZ pigs, including 27 upregulated and 45 downregulated miRNAs, 64 upregulated and 119 down-regulated lncRNA, 814 upregulated and 556 downregulated mRNAs. QPCR analysis exhibited strong consistency with the sequencing data. GO and KEGG analysis elucidated that the differentially expressed transcripts were mainly associated with cell growth and death, signal transduction, peroxisome proliferator-activated receptors (PPAR), AMP-activated protein kinase (AMPK), PI3K-Akt, adipocytokine and foxo signaling pathways, all of which are strongly involved in cell development, lipid metabolism and adipogenesis. Further analysis indicated that the BGIR9823_87926/miR-194a-5p/AQP7 network may be effective in the process of adipocyte differentiation or adipogenesis. Conclusion: Our study provides comprehensive insights into the regulatory network of backfat deposition and lipid metabolism in pigs from the point of view of miRNAs, lncRNAs and mRNAs.

Development of a Primary Tissue Culture Method having Greater Reliability than Isolated Cell Cultures - Steroid-Responsiveness of Uterine Myometrial and Myomatous(Leiomyomatous) Cells (자궁근종세포의 최적 초기배양 조건 확립 - 정상 자궁근세포와 자궁근종세포의 스테로이드에 대한 반응)

  • Lee, Eun-Ju;Bajracharya, Prati;Hyun, Jin-Hee;Kim, Hang-Jin;Song, Gun-Ho;Cho, Kyung-Hyun;Lee, Dong-Mok;Lee, Taek-Hoo;Chun, Sang-Sik;Choi, In-Ho
    • Development and Reproduction
    • /
    • v.11 no.3
    • /
    • pp.205-217
    • /
    • 2007
  • As an initial step toward better understanding of the molecular mechanism of estrogen-dependent growth in myoma, an optimal primary cell culture condition has been developed and examined by this study. Myoma and myometrium were cultured by two different methods. Culture stability and $E_2$-responsiveness in stable culture were studied. The culture of digested tissue pieces(Method 2) was found to be a stable culture method for the myoma and myometrium showing a favorable response to estrogen. mRNA expression of PR, IGF-1 and IGF-1 receptor genes was enhanced by $E_2$. The gene responses to $E_2$ were higher in myoma compared with myometrium. Moreover, these responses were more expressive in tissues than in the surrounding cells in primary culture of normal myometrium and myoma, implying a vital role of cell communication through the extracellular matrix in maintaining the estrogen-responsiveness. The development of an improved cell culture system for myoma provides an in vitro tool to further investigate the basis of the tumor formation.

  • PDF

Employees' Humor Style, Emotional Labor, Job Satisfaction, and Turnover Intention among High-Contact Service Employees (고접촉 서비스 종업원의 유머 스타일과 감정노동 및 직무만족, 이직의도와의 관계에 관한 연구)

  • Kim, Yu-Kyung
    • Journal of Distribution Science
    • /
    • v.12 no.10
    • /
    • pp.109-121
    • /
    • 2014
  • Purpose - Most studies related to emotional labor have been conducted in the low-contact service industry rather than the high-contact service industry. Since the subjects of this study were golf service assistants, working in an industry in which the interaction and contact between customers and employees are considerable and the degree of customization is high, this study is significant. Thus, we would like to focus on the relationship between the humor style of golf service assistants and emotional labor. Humor style was divided into two styles-adaptive humor and maladaptive humor. Emotional labor was divided into two dimensions-surface acting and deep acting. In addition, we identify the relationship between emotional labor, job satisfaction, and turnover intention. Research design, data, & methodology - This study focuses on the humor styles in the golf service industry, among golf service assistants, on their emotion labor, and verifies the relationship between emotion labor and work satisfaction or job turnover intention. Based on the outcome, a total of four hypotheses were set up. To verify the hypotheses, surveys were conducted on the golf service assistants working in two golf fields in the region of Busan as well as its neighboring area. A total of 250 copies of the questionnaire were distributed. Subsequently, a total of 227 valid questionnaire copies, after excluding improper responses, were used in the analysis. After verifying the reliability and feasibility of variances, Amos 18.0 was used to implement the structure method so as to verify the study hypotheses. As a result of this analysis, the suitability of the entire model was considerably appropriate to the standard value, and the level is adequate to accommodate the study model completely. Results - First, it was found that the adaptive humor of golf service assistants had a positive effect on deep acting and maladaptive humor had a positive effect on surface acting. Second, It was found that employees' satisfaction with their job was reduced through surface acting, and those employees who experienced positive emotional labor in the form of deep acting were more satisfied with their job. Third, surface acting among employees increased turnover intention, but deep acting reduced it. Finally, the relationship between job satisfaction and the turnover intention of golf service assistants in the golf service industry was examined. Conclusions - First, the adaptive humor of the golf assistants in the golf service industry had a positive effect on their expression behavior. Second, deep acting increased their job satisfaction while their surface acting had a noticeably negative effect on their job satisfaction. Third, the surface acting of the emotion labor dimensions that the golf service assistants experience increases their turnover intention while their deep acting decreases their turnover intention. Finally, when analyzing the relationship between job satisfaction and turnover intention of the golf service assistants, it was found that the turnover intention decreased when their job satisfaction is increased.

Analysis of Middle School Students' Difficulties in Science Inquiry Activity in View of Knowledge and Information Processing Competence (지식정보처리역량 관점에서 중학생들의 과학탐구활동 어려움 분석)

  • Son, Mihyun;Jeong, Daehong;Son, Jeongwoo
    • Journal of The Korean Association For Science Education
    • /
    • v.38 no.3
    • /
    • pp.441-449
    • /
    • 2018
  • The knowledge and information processing competence is one of the essential competencies in an information-oriented society which is highly related to science education. The purpose of this study is to investigate middle school students' difficulties in science inquiry activity in view of the knowledge and information processing competence. Collection, processing, and application of information are extracted as common elements of the knowledge and information processing competence through literature search. Data were obtained from eight students of a middle school in Seoul for five months and were analyzed based on constant comparative method to extract students' difficulties. As a result, the element of information collection is observed through science inquiry and the element of information processing is observed in hypothesis setting step, inquiry result analysis step, and inquiry result sharing step. The element of information application is observed in the creation of posters for sharing and inquiry reports. Difficulties in information collection were found in lack of confidence in information gathering, systematic search strategy, and information reliability. Difficulties in information processing appeared in the inexperience of analysis program use, information transformation, and interpretation of linkage information. Difficulties in information application were caused by the lack of effective organization and effective expression of information. In this study, it is meaningful to provide a clue as to how the knowledge and information processing competence can be established in science education.

A Study on the Use of Generative AI and Learner Experience for Jewelry Design Education (주얼리 디자인 교육을 위한 생성형 AI의 활용 및 학습자 경험 연구)

  • Hye-Rim Kang
    • The Journal of the Convergence on Culture Technology
    • /
    • v.10 no.5
    • /
    • pp.743-749
    • /
    • 2024
  • Recently, the use of generative AI has become more active in university education, but jewelry design education and research using generative AI are still insufficient. Accordingly, we would like to discuss the possibilities and limitations of visualization of jewelry design ideation and expression using generative AI in the jewelry design development education and ideation stage, as well as the experience and application of generative AI by college students. To analyze the impact of generative AI on the learning experience, it was analyzed from the perspectives of 'usability', 'usefulness', 'reliability', and 'satisfaction'. As a result, generative AI confirmed positive results in terms of usability and usefulness to trainees, and confirmed the potential for effects using personalized education and collective intelligence. The combination of jewelry design education and generative AI is part of convergence education, and the significance of this study is to lay the foundation for effective use in jewelry design education by analyzing learners' experiences and perceptions of using generative AI. This kind of education reflects the trends of the times for nurturing future society's talents and will contribute to the promotion of learners' broad creative thinking.

3D Mesh Reconstruction Technique from Single Image using Deep Learning and Sphere Shape Transformation Method (딥러닝과 구체의 형태 변형 방법을 이용한 단일 이미지에서의 3D Mesh 재구축 기법)

  • Kim, Jeong-Yoon;Lee, Seung-Ho
    • Journal of IKEEE
    • /
    • v.26 no.2
    • /
    • pp.160-168
    • /
    • 2022
  • In this paper, we propose a 3D mesh reconstruction method from a single image using deep learning and a sphere shape transformation method. The proposed method has the following originality that is different from the existing method. First, the position of the vertex of the sphere is modified to be very similar to the 3D point cloud of an object through a deep learning network, unlike the existing method of building edges or faces by connecting nearby points. Because 3D point cloud is used, less memory is required and faster operation is possible because only addition operation is performed between offset value at the vertices of the sphere. Second, the 3D mesh is reconstructed by covering the surface information of the sphere on the modified vertices. Even when the distance between the points of the 3D point cloud created by correcting the position of the vertices of the sphere is not constant, it already has the face information of the sphere called face information of the sphere, which indicates whether the points are connected or not, thereby preventing simplification or loss of expression. can do. In order to evaluate the objective reliability of the proposed method, the experiment was conducted in the same way as in the comparative papers using the ShapeNet dataset, which is an open standard dataset. As a result, the IoU value of the method proposed in this paper was 0.581, and the chamfer distance value was It was calculated as 0.212. The higher the IoU value and the lower the chamfer distance value, the better the results. Therefore, the efficiency of the 3D mesh reconstruction was demonstrated compared to the methods published in other papers.

Thermodynamics-Based Weight Encoding Methods for Improving Reliability of Biomolecular Perceptrons (생체분자 퍼셉트론의 신뢰성 향상을 위한 열역학 기반 가중치 코딩 방법)

  • Lim, Hee-Woong;Yoo, Suk-I.;Zhang, Byoung-Tak
    • Journal of KIISE:Software and Applications
    • /
    • v.34 no.12
    • /
    • pp.1056-1064
    • /
    • 2007
  • Biomolecular computing is a new computing paradigm that uses biomolecules such as DNA for information representation and processing. The huge number of molecules in a small volume and the innate massive parallelism inspired a novel computation method, and various computation models and molecular algorithms were developed for problem solving. In the meantime, the use of biomolecules for information processing supports the possibility of DNA computing as an application for biological problems. It has the potential as an analysis tool for biochemical information such as gene expression patterns. In this context, a DNA computing-based model of a biomolecular perceptron has been proposed and the result of its experimental implementation was presented previously. The weight encoding and weighted sum operation, which are the main components of a biomolecular perceptron, are based on the competitive hybridization reactions between the input molecules and weight-encoding probe molecules. However, thermodynamic symmetry in the competitive hybridizations is assumed, so there can be some error in the weight representation depending on the probe species in use. Here we suggest a generalized model of hybridization reactions considering the asymmetric thermodynamics in competitive hybridizations and present a weight encoding method for the reliable implementation of a biomolecular perceptron based on this model. We compare the accuracy of our weight encoding method with that of the previous one via computer simulations and present the condition of probe composition to satisfy the error limit.

Development of Sorption Database (KAERI-SDB) for the Safety Assessment of Radioactive Waste Disposal (방사성폐기물 처분안전성 평가 자료 제공을 위한 핵종 수착 데이터베이스(KAERI-SDB) 개발)

  • Lee, Jae-Kwang;Baik, Min-Hoon;Jeong, Jongtae
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
    • /
    • v.11 no.1
    • /
    • pp.41-54
    • /
    • 2013
  • Radionuclide sorption data is necessary for the safety assessment of radioactive waste disposal. However the use of sorption database is often limited due to the accessability. A web-based sorption database program named KAERI-SDB has been developed to provide information on the sorption of radionuclides onto geological media as a function of geochemical conditions. The development of KAERI-SDB was achieved by improving the performance of pre-existing sorption database program (SDB-21C) developed in 1998 and considering user's requirements. KAERI-SDB is designed that users can access it by using a web browser. Main functions of KAERI-SDB include (1) log-in/member join, (2) search and store of sorption data, and (3) chart expression of search results. It is expected that KAERI-SDB could be widely utilized in the safety assessment of radioactive waste disposal by enhancing the accessibility to users who wants to use sorption data. Moreover, KAERI-SDB opened to public would also improve the reliability and public acceptance on the radioactive waste disposal programs.

A Study on the Analysis of Mediated Effects of Father's Play Participation in the Relationship Between Father's Play Belief and Child's Playfulness (아버지의 놀이신념과 유아 놀이성 관계에서 아버지의 놀이 참여도의 매개효과 분석)

  • Choi, Tae-Sun
    • Journal of Korea Entertainment Industry Association
    • /
    • v.14 no.7
    • /
    • pp.333-342
    • /
    • 2020
  • Children grow and develop through play. Play is being used as a friend and a learning material and tool in everyday life of children. It is a research study aimed to empirically analyze the effects of father's play belief and play participation on child's playfulness. A survey was carried out for the selected 284 children aged 5 years and older attending kindergarten located in D-gu, Seoul in order to achieve the goal of this study. Frequency analysis, descriptive statistics, reliability verification, correlation analysis, and regression analysis were conducted on the data collected through the survey by using the SPSS program, and the research results were derived as below. First, it showed that father's play belief and play participation have a positive effect on child's playfulness of physical spontaneity, social spontaneity, cognitive spontaneity, expression of joy, and a sense of humor. Second, father's play participation had a significant effect as a partial parameter in the relationship between father's play belief and child's playfulness. Thus, it was drawn the conclusion that father's play belief and play participation are important variables that can predict child's playfulness. Therefore, It will be necessary to promote children's playfulness development by continuously providing parent education programs on creating an atmosphere of various interaction at home through plays in the relationship between father and child and a desirable role of father in the play activities with child at the level of preschool educational institutions.

A Study on Color Image of TV News Anchor Woman's Jackets (TV 뉴스 여성앵커 재킷의 색상 이미지 연구)

  • Lee, Eun-Kyung
    • Korean Journal of Human Ecology
    • /
    • v.19 no.1
    • /
    • pp.149-156
    • /
    • 2010
  • TV news anchor woman's appearance, voice, expression, and clothing, etc., have an influence on the reliability of the article to be reported. Among these, clothing is the most crucial factor in forming an anchor woman's image, especially the clothing color factor. This study is aimed at providing the basic foundation for anchor woman when they select the clothing color by analyzing the clothing color image on the screen. For this purpose, the KBS and MBC 9 o'clock news desk and SBS 8 o'clock news of the local major news programs were selected. With the collection of 300 pieces of news clips related to anchor woman's clothing from January to December 2008, they were classified into F/W seasons and analyzed by the clothing color. The surveying method of clothing color was to capture the anchor woman's clothing among the news clips, then pick the representing color by applying Adobe Photoshop, and researching the formed $L^*a^*b^*$ value of color chips. The surveyed color was transformed into value of distant cell, H V/C, and the results were analyzed. As a result, it showed that the White system for anchor woman's clothing during the S/S seasons is most frequently picked, followed by the Red system. In F/W seasons, Gray system is the most favored, then White and Red, respectively. It was revealed that the most frequently selected colors for upper-wear by anchor women in the three broadcasting stations was an achromatic color, such as White or Gray, and then the chromatic color, Red. It shows that there is no big difference in season. The Inner-wear color matched the jackets which were also achromatic in color, white and black being the most favored in the S/S seasons, and in the case of chromatic colors, Red was the most favored. In addition to this, identical coloration with jacket, coloration with similar color, or single color as clothing color were no less frequently adopted. During the F/W seasons, identical coloration accounts for 26%, the most popular colored being White and Red. It was found that the coloration with achromatic colors are highly favored in the three major broadcasting stations alike.