• KSBB Journal
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• v.13 no.6
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• pp.662-668
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• 1998

Viruses belonging to the Hantavirus genus cause two acute severe illness in humans, i.e., Haemorrhagic Fever with Renal Syndrome (HFRS) and Hantavirus Pulmonary Syndrome(HPS). Among them, Hantaan virus is one of the most important viruses causing HFRS. Recombinant expression vectors, pKK-NP and pET-NP, with Hantaan viral nucleocapsid gene were constructed, and used to transform Eschericia coli BL21(DE3). Stability of the vectors in the host strain, and effects of some environmental conditions on the expression of nucleocapsid gene were studied. Expression vector, pKK-NP, was very unstable, and the expression level of nucleocapsid gene was very low compared to that of pET-NP. BL21(pET-NP) produced about 100 mg of N protein per liter of culture broth. Induction time did not show any significant difference on the expression level of nucleocapsid gen and cell growth. BL21(pET-NP) culture at 35$^{\circ}C$ showed a little higher expression level than at 30$^{\circ}C$ during growth phase, but reached to the same level at stationary phase. Total expression level was proportional to supplemented glucose concentration of media up to 0.5% along with cell growth, but expression level per unit cell mass was inversely proportional to glucose concentration and maximal when glucose was not supplemented at all.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2004.11a
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• pp.138-149
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• 2004

In this study, we analyzed the gene expression data of Saccharomyces cerevisiae obtained from Holstege et al. 1998 to understand the relationship between expression level and nucleotide sequence of a gene. First, the correlation between gene expression and percent composition of each type of nucleotide was computed. It was observed that nucleotide 'G' and 'C' show positive correlation (r ${\geq}$ 0.15), 'A' shows negative correlation (r ${\approx}$ -0.21) and 'T' shows no correlation (r ${\approx}$ 0.00) with gene expression. It was also found that 'G+C' rich genes express more in comparison to 'A+T' rich genes. We observed the inverse correlation between composition of a nucleotide at genome level and level of gene expression. Then we computed the correlation between dinucleotides (e.g. AA, AT, GC) composition and gene expression and observed a wide variation in correlation (from r = -0.45 for AT to r = 0.35 for GT). The dinucleotides which contain 'T' have wide range of correlation with gene expression. For example, GT and CT have high positive correlation and AT have high negative correlation. We also computed the correlation between trinucleotides (or codon) composition and gene expression and again observed wide range of correlation (from r = -0.45 for ATA r = 0.45 for GGT). However, the major codons of a large number of amino acids show positive correlation with expression level, but there are a few amino acids whose major codons show negative correlation with expression level. These observations clearly indic ate the relationship between nucleotides composition and expression level. We also demonstrate that codon composition can be used to predict the expression of gene in a given condition. Software has been developed for calculating correlation between expression of gene and codon usage.

• Proceedings of the Korean Institute of Building Construction Conference
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• 2016.05a
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• pp.253-254
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• 2016

In the trend of building enlargement, high-rise and irregular shaped building, domestic application of BIM in construction phase is increasing in recent years. BIM that is based on a three-dimensional, requires a specific building expression. Model expression level required for each phase such as design, construction, and maintenance of the construction project can be different. BIM model to apply in practice is difficult because it is not clear to set up the criteria for the expression level of construction BIM model. And it has a limit that is lower productivity in construction project. Therefore, this study considers the way of the improvement and analyzes the application for representation level of construction BIM model through the survey. By analyzing of the survey results, this study will be to present a basis for an appropriate expression level setting of construction BIM model.

• Journal of Genetic Medicine
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• v.3 no.1
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• pp.33-37
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• 1999

Recombinant adenovirus vector systems with strong promoters have been used to achieve high level production of recombinant protein. However, this overexpression system cause some problems such as disturbance of cell physiology and increment of cellular toxicity. Here, we showed a tetracycline-regulated adenovirus expression vector system. Our results showed that the expression level of transgene(p-53) was high and easily regulated by tetracycline. In addition, the maximal gene expression level of the tetracycline-controlled gene expression system was higher than that of the wild type CMV promoter system. Therefore, tetracycline-regulated adenoviral vector system could be applicable for regulatory high-level expression of toxic gene. Also, this system will be useful for functional studies and gene therapy.

• Korean Journal of Child Studies
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• v.28 no.3
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• pp.19-44
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• 2007

This study used the hypothetical path model to investigate individual traits(attachment, reciprocal relationship, and self-esteem), situational evaluation(justification, and intention) and jealousy level in adolescents. Subjects were 853 7th, 9th, 11th grade students in the city of Daegu. Results showed that scores of girls were higher than boys on self-reported and peer-reported jealousy level, attachment, reciprocal relationship, adaptive expression and non-adaptive expression. Scores of boys were higher than girls for self-esteem, justification. Self-reported jealousy level was affected by situation evaluation(justification and intention) and attachment. Peer-reported jealousy level was affected by reciprocal relationship. Jealousy expression modes were affected by self-reported jealousy level and attachment.

• Development and Reproduction
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• v.24 no.3
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• pp.167-176
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• 2020

The fat pad defined as the epididymal fat is located at the head part of the epididymis. However, another fat mass is present near the caudal epididymis, named tail epididymal fat. The present research was focused to determine the expression of adipocyte-associated molecules in the mouse tail epididymal fat at different postnatal ages, including 2, 5, 8, and 12 months of age. The quantitative real-time PCR analysis showed continuous increases of expression levels of delta like non-canonical Notch ligand 1, leptin, and resistin as postnatally aged. The transcript level of peroxisome proliferator-activated receptor gamma was the highest at 5 months of age, remaining at a steady level until 12 months of age. Expression levels of fatty acid binding protein 4, leptin, and adiponectin were significantly increased until 8 months of age, persisting the level at 12 months of age. The transcript level of fatty acid synthase was significantly increased at 8 months of age, without a further change of the level at 12 months of age. These findings exhibited the expression of adipocyte-associated genes which were also detected at the ordinary epididymal fat pad. However, expression patterns of these genes in the tail epididymal fat are different with those in the distal and proximal epididymal fat, suggesting distinct characteristics and/or functions of the tail epididymal fat.

• Microbiology and Biotechnology Letters
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• v.19 no.1
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• pp.37-44
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• 1991

- I aimed to isolate trans-acting factors involved in the basal expression level of eukaryotic genes. One of the yeast histidine biosynthetic gene, HIS5 was taken as a model for this study. HIS5 gene has a substantial basal level in amino acid rich medium and is derepressed if starved for any single amino acid. The derepression is mediated by cis-acting DNA sequences 5'-TGACTC-3' found in 5' non-transcribed region of the gene and trans-acting factors including GCN4 as positive factor and its negative factor GCDI 7, and GCNZ as a negative factor of GCD17. I first investigated the role of these trans-acting factors in HIS5 basal expression level by using HIS5-pH05 fusion in which expression of pH05 gene encoding inorganic phosphate-repressible acid phosphatase (APase) is regulated by HIS5 promoter. Strain with gcn2 or gcn4 mutation showed 3 to 4 fold lower APase activity than wild type. The level of APase activity was similar in gcn2 and gcn4 mutants. Trans-acting factors involved in basal level were identified by isolating 14 mutants showing increased expression of HISSPH05 fusion from gcn4 background. All the mutants carry a single nuclear recessive mutation and fall into four complementation groups, designated as bell (basal expression level), be12, be23 and be14.

• Journal of fish pathology
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• v.16 no.3
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• pp.147-152
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• 2003

The CMV promoter driven luciferase reporter gene coding plasmid (pcDNA-luc) was constructed and used as a model for DNA immunization study. Expression of the recombinant luciferase protein was confirmed in vitro in RTG-2 cell line before using in vivo study in olive flounder. In dose response study, the maximum expression of the luciferase gene was found in the group injected with 10-15μg of plasmid DNA. The kinetic study showed that the luciferase gene expression was reached at the maximum level at one day after injection and slightly decreased after then but significantly high level of expression was sustained until the conducted experiment of 7 days. In the study of tissue distribution of gene expression, it was found that luciferase gene was expressed at the significant level in immune organs such as gill and spleen, located far from the injected site, suggesting the systemic distribution of the intramuscularly injected DNA in olive flounder.

• The Journal of Korean Obstetrics and Gynecology
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• v.23 no.3
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• pp.38-55
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• 2010

Purpose: This study was designed to find out the anti-cancer effects of Samultang-Gami which was composed of Rehmanniae Radix(RR), Angelicae Gigantis Radix(AGR), Cnidii Rhizoma(CR), Paeoniae Radix(PR), Cortex Moutan Radicis(CMR), Hedyotis Diffusa(HD) and Caesalpinia Sappan on HeLa, HepG2 and AGS cells. Methods: Various cancer cell lines including HeLa, HepG2 and AGS cells, were used. In vitro anti-cancer effects were measured by MTT assay using cancer cell lines treated with various concentrations of 70% ethanol extract of Samultang-Gami. Expression of cell cycle arrest mediators including Bax, Bcl-2, p53 and DARP-1 proteins were measured by Western blot analysis. Results: 1. Samultang-Gami decreased the viability of HeLa and HepG cells in a dosedependent manner. 2. AGR, CMR, PR and HD decreased the viability of HeLa, HepG2 and AGS cells. 3. We could observe that the decreased Bax and Bcl-2 expression level and the increased PARP-1 expression level by Samultang-Gami extracts treated in HeLa cells. 4. We could observe that the decreased Bcl-2 expression level and the increased Bax, p53 and PARP-1 expression level by RR extracts treated in HeLa cells. and also could observe that the reduction of the protein level of Bcl-2, p53 and PARP-1 and the increase of the protein level of Bax by PR in HeLa cells. 5. We could observe that the increased p53 expression level, the decreased PARP-1's that and the unchanged Bax and Bcl-2's that by Samultang-Gami extracts treated in HepG2 cells. 6. We could observe that the reduced Bcl-2 expression level by each of RR extracts and PR extracts in HepG2 cells. 7. The treatment of Samultang-Gami in AGS cells didn't have any effect on the expression level of Bax, Bcl-2, p53 and PARP-1. 8. We could observe that the increased p53 and PARP-1 expression level by each of CR, RR and PR extracts in AGS cells. Conclusion: Taken together, we suggest that Samultang-Gami exhibits cytotoxic effects on HeLa, HepG2 and AGS cells, causing apoptosis. The results showed that Samultang-Gami may do so by regulating the expression of specific target molecules that promote efficient apoptotic cell death in a dose-dependent manner.

• Journal of dental hygiene science
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• v.7 no.2
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• pp.89-96
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• 2007

The purpose of this study was to examine the relationship between the self-expression level of dental hygiene students related to communicative competence and their stress during clinical practice and what affected their stress. The subjects in this study were 125 dental hygiene students in W college, on whom a survey was conducted from September 18 through 30, 2006. After the collected data were analyzed with SPSS WIN 10.0 program, the following findings were acquired: 1. When a factor analysis was made to evaluate the self-expression of the students, there appeared three different categories of self-expression: voice/content, facial expression/attitude and sentiment. The three made a 58.1% prediction of their self-expression. As for overall reliability, they turned out highly reliable(Cronbach'a = .881). 2. The dental hygiene students got a mean of 3.58 out of possible five points in self-expression, which indicated that they expressed themselves relatively well. Concerning connections between their general characteristics and self-expression level, those who were inactive during clinical practice got a mean of 3.28, whereas the others who were active got a mean of 3.85. It implied that those who took a more active attitude to clinical practice expressed themselves better(p < .01). The person with whom they found it hard to get along made a statistically significant difference to their self-expression(p < .05). The students who didn't fare well with dental hygienists got the best score(3.70). The second best group(3.53) didn't get along with dentists, followed by assistant nurses(3.46) and patients/caregivers(3.31). As for the impact of the field of dream job, the students who hoped to work or study overseas(4.21) excelled in self-expression those who wanted to be hired in a general hospital, to go onto a school of higher grade and to work in a public dental clinic(p < .05). Among the general characteristics, satisfaction level with major, health status and motivation of choosing dental hygiene made no statistically significant differences to their self-expression. 3. Regarding relations between self-expression level and stress about clinical practice, those who didn't express themselves properly in terms of sentiment scored higher in stress level(3.65). Their stress was statistically significantly different according to self-expression level (p < .05). 4. As for the influence of self-expression and general characteristics on stress with clinical practice, sentiment was selected from among the self-expression categories as a decisive factor to affect stress. Their stress varied statistically significantly with that(p < .05). In contrast, their demographic variables made no statistically significant difference to that, which made a 79.2% prediction of it.